• 제목/요약/키워드: culture temperature

검색결과 2,377건 처리시간 0.026초

가수분해방법에 의한 식물세포배양여액으로부터 Paclitaxel 수율증가 (method of Using Hydrolysis to Increase Paclitaxel Yield from plant Cell Culture)

  • 김진현
    • KSBB Journal
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    • 제15권4호
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    • pp.402-404
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    • 2000
  • This work is method that uses a hydrolysis for increasing yield of paclitaxel in plant cell cultures. The best pH is 3.0 to obtain a maximum yield at fixed reaction temperature and time t pH 3.0 reaction temperature 80$^{\circ}C$ and reaction time 8 hr give the highest yield which is three time of control. This is very simple and efficient method to increase paclitaxel yield in plant cell cultures.

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소형 rotifer, Proales similis의 대량배양을 위한 최적 염분 및 수온 조건 (Optimal Salinity and Temperature Conditions for Mass Culture of Small Rotifer, Proales similis)

  • 이배익;김대중;김신권;이남실;;권오남;박흠기;박진철
    • 수산해양교육연구
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    • 제26권6호
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    • pp.1315-1321
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    • 2014
  • We investigated the optimum salinity and temperature conditions for the mass culture of small rotifer, Proales similis. In the salinity experiment ranging from 1, 5, 10, 15, 20, 25 and 33‰, growth tended to decrease with salinity increase. Most agreeable salinity for rotifer growth was 1‰ in which maximum density and specific growth rate (SGR) were obtained. In the temperature experiments ranging from 15, 20, 25, 30 and $35^{\circ}C$, continuous growth of rotifer populations was found up to $35^{\circ}C$. The highest maximum density (2,060 inds./mL) of rotifer was observed at $25^{\circ}C$ in given temperature regime. Also, the SGR of females showed increasing tendencies with the increase of temperature. These results suggest that the optimum salinity and temperature for mass culture of P. similis may be 1‰ and $25^{\circ}C$, respectively.

Effect of Far-Infrared Finishing on Brassiere Pad

  • Shin Jung-Sook
    • The International Journal of Costume Culture
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    • 제8권2호
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    • pp.124-131
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    • 2005
  • This study focused on the change of skin temperature by the emissivity and emission power of far-infrared for conformant far infrared effect to naked eyes. The study method is to manufacture the bra pad by each concentration on far-infrared materials of illite powder $(K,H_3O)AI_2(Si,Al)_4O_{10}(H_2O,OH)_2)$, liquid alumina ($Al_2O_3$), the extracted liquid from 29 kind of medical plants, then, measured change of skin temperature. Result are as follows. Far-infrared were emitted each $90.2\%,\;90.1\%,\;89.7\%$ from the illite powder, liquid alumina, extracted liquid from medical plants. When the testee weared the bra pad, the temperature of coated bra pad was $0.5^{\circ}C$ higher than the non finished bra pad. Washing fastness on far-infrared finishing was better dope addition method than coating method.

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A comparing on the use of Centrifugal Turbine and Tesla Turbine in an application of Organic Rankine Cycle

  • Thawichsri, Kosart;nilnont, Wanich
    • International Journal of Advanced Culture Technology
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    • 제3권2호
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    • pp.58-66
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    • 2015
  • This paper aims to compare the use of Centrifugal Turbine and Tesla Turbine in an application of Organic Rankine Cycle (ORC) Machine using Isopentane as working fluid expanding. The working fluid has boiling point below boiling water and works in low-temperature sources between $80-120^{\circ}C$ which can be produced from waste heat, solar-thermal energy and geothermal energy etc. The experiment on ORC machine reveals that the suitability of high pressure pump for working fluid has result on the efficiency of work. In addition, Thermodynamics theory on P-h diagram also presented the effect of heat sources' temperature and flow rate on any work. Thus, the study and design on ORC machine has to concern mainly on pressure pump, flow rate and optimized temperature. Result experiment and calculate ORC Machine using centrifugal Turbine efficiency better than Tesla turbine 30% but Tesla Turbine is cheaper and easily structure. Further study on the machine can be developed throughout the county due to its low cost and efficiency.

Numerical Simulation of Thermal Fluctuation of Hot and Cold Fluids Mixing in a Tee Junction

  • Gao, Kai;Lu, Tao
    • International Journal of Advanced Culture Technology
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    • 제3권2호
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    • pp.171-178
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    • 2015
  • In this work, mixing processes of hot and cold fluids of three different jet types are predicted by large-eddy simulation (LES) on FLUENT platform. Temperature at different positions of internal wall and mixing conditions of T-junctions at different times are obtained, then the simulated normalized mean and root-mean square (RMS) temperature, temperature contour and velocity vector of every case are compared. The results indicate that, the mixing regions in the tee junction is related to the jet type, and temperature fluctuations on the pipe wall in the type of the deflecting jet is the least.

Realistic Soap Bubble Appearance using Background Scene and Kelvin Temperature Matching

  • Yoo, Sangwook;Chin, Seongah
    • International Journal of Advanced Culture Technology
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    • 제9권3호
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    • pp.265-270
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    • 2021
  • VR and AR contents provide a rich user experience [1]. Realistic content with human computer interaction and immersion provides an improved user experience, but there is a limit to producing all elements realistically. In this study, we propose a method to advance the rendering of immersive content using background color information [2]. First, the elements necessary for Kelvin temperature rendering are derived from the color and background as context elements, and the rendering effect has been realized in the soap bubble. For soap bubbles Kelvin temperature rendering, the average color of the background is extracted and the color with the highest similarity is applied by comparing the main color and Kelvin temperature.

The nocturnal characteristics of Seoul city: Focused on light color

  • Sung Dae Hong
    • International Journal of Advanced Culture Technology
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    • 제12권2호
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    • pp.353-359
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    • 2024
  • The color temperature and light color of nighttime lighting in a particular city is an important factor in determining its nighttime identity. To quantitatively analyze the nocturnal characteristics of Seoul, this study focuses on the light color of the lighting sources that used in the places included in the Seoul Night View 100 Photobook. As a result, the color temperature of white light in the surveyed places is in the range of 2,500~3,500K, of which 3,000~3,500K represents the highest proportion. In addition, the color temperature in the 2,500~3,500K range was found to be evenly distributed across the five surveyed regions. Apart from white light, blue color hue accounts for a high percentage in the monochromic light category, and the excitation purity was measured to be 71.6% on average. In addition, 46% of the buildings with monochromic light are in urban centers.

왕겨 소각로 배연가스 이용을 위한 미세죠류 배양 조건 확립 (Microalgal Culture Conditions for Utilization of Flue Gas from Rice Husk Incinerator)

  • 박승제;조성호;이진석;정용섭
    • Journal of Biosystems Engineering
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    • 제24권1호
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    • pp.9-18
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    • 1999
  • This study was performed to investigate the optimum microalgal culture conditions using flask culture and to find the feasibility of using the flue gas of the rice husk incinerator for cultivating the microalgae. The optimum initial pH of media was 4.5 for the microalgae culture, and the intermittently illuminated culture was more effective than the continuous illuminated culture. Thus, the balance between photosynthesis and formative metabolism must be considered thoroughly to cultivate microalgal cells. The optimum CO2 concentrations were in the range of 7 to 10%, and the optimum temperature was about 35$^{\circ}C$ in both the daytime and the nighttime for the culture. When flue gas of the rice husk incinerator was applied to the microalgae culture using stirred photobioreactor, the dry cell weight was 0.026 g dry biomass/hr$.$l. The results obtained in experiments indicated that the flue gas was effective for microalgae culture without any limitations.

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Growth of Issatchenkia orientalis in Aerobic Batch and Fed-batch Cultures

  • Shin, Hyung-Tai;Lim, Yoo-Beom;Koh, Jong-Ho;Kim, Jong-Yun;Baig, Soon-Yong;Lee, Jae-Heung
    • Journal of Microbiology
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    • 제40권1호
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    • pp.82-85
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    • 2002
  • The aerobic batch growth of Issatchenkia orientalis DY252 with glucose and fructose medium was investigated at 32$\^{C}$ and pH 5.0. Aerobic ethanol production was evident with yeast I, orientalis. A diauxic lag of about 1 h between growth on glucose and growth on ethanol during batch culture was observed. However, no diauxic growth occurred with fructose. As the incubation temperature was increased from 32 to 39$\^{C}$, viability at the end of each batch culture declined significantly, from 93 to 43%, Unlike the effect of temperature, viability was not greatly affected by incubation pH, and cell yield values in a range of 0.45-0.48 were obtained. In order to overcome overflow metabolism, a fedbatch culture under glucose limitation was carried out. Compared with aerobic batch culture, about 10% improvement in cell yield was achieved with a fed-batch culture in optimal conditions.

Optimized Culture Conditions for Production of the chimaeric protein, Uropathogenic Escherichia coli Adhesin - Cholera Toxin A2B Subunits, in Escherichia coli TB1

  • Lee, Yong-Hwa;Kim, Byung-Oh;Rhee, Dong-Kwon;Pyo, Suh-Kneung
    • Biomolecules & Therapeutics
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    • 제12권3호
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    • pp.179-184
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    • 2004
  • The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause for urinary tract infections. In our previous study, the Adhesin/CTXA2B was expressed as soluble recombinant chimaeric protein derived from the uropathogenic Escherichia coli adhesin genetically coupled to cholera toxin A2B (CTXA2B) subunit in Escherichia coli. Since it is very important to optimize IPTG concentration and culture temperature to maximize cell growth and productivity, These optimal culture factors were determined to increase the productivity of the expressed Adhesin/CTXA2B chimaeric protein in Escherichia coli TB1 carrying pMALfimH/ctxa2b. Our data demonstrate that optimal concentration of IPTG for increased production of chimaeric protein was 0.5 mM. Additionally, culture time was 10 hours and temperature, 37${\circ}C$.