method of Using Hydrolysis to Increase Paclitaxel Yield from plant Cell Culture

가수분해방법에 의한 식물세포배양여액으로부터 Paclitaxel 수율증가

  • 김진현 ((주)삼양제넥스 생명공학연구소)
  • Published : 2000.08.01

Abstract

This work is method that uses a hydrolysis for increasing yield of paclitaxel in plant cell cultures. The best pH is 3.0 to obtain a maximum yield at fixed reaction temperature and time t pH 3.0 reaction temperature 80$^{\circ}C$ and reaction time 8 hr give the highest yield which is three time of control. This is very simple and efficient method to increase paclitaxel yield in plant cell cultures.

Keywords

References

  1. J. of the National Cancer Institute v.82 Review; Taxol : A Novel Investigational Antimicrotubule Agent Rowinsky, E.K.;L.A. Cazenave;R.C. Donehower
  2. J. Nat. Prod. v.53 The Chemistry of Taxol, A Clinically Useful Anticancer Agent Kingston, D.G.I.;G. Samaranayake;C.A. Ivey
  3. Nature v.367 Total Synthesis of Taxol Nicolaou, K.C.;Z. Yang;J.J. Llu;H. Ueno;P.G. Nantermet;R.K. Guy;C.F. Clalborne;J. Renaud;E.A. Couladouros;K. Paulvannan;E.J. Sorensen
  4. J. of American Chemical Society v.116 First Total Synthesis of Taxol.1.Functionalization of the B Ring Holton, R.A.;C. Somoza;H.B. Kim;F. Liang;R.J. Biediger;P.D. Boatman;M. Shindo;C.C. Smith;S. Kim;H. Nadizadeh;Y. Suzuki;C. Tao;P. Vu;S. Tang;P. Zhang;K.K. Murthi;L.N. Gentile;J.H. Liu
  5. J. of American Chemical Society v.116 First Total Synthesis of Taxol. 2.Completion of the C and D Ring Holton, R.A.;H.B. Kim;C. Somoza;F. Liang;R.J. Biediger;R.J. Biediger;P.D. Boatman;M. Shindo;C.C. Smith;S. Kim;H. Nadizadeh;Y. Suzuki;C. Tao;P. Vu;S. Tang;P. Zhang;K.K. Murthi;L.N. Gentile;J.H. Liu
  6. U.S.Patent, 5,871,979 Method for Mass Production of Taxol by Semi-Continuous Culture with Taxus chinensis Cell Culture Choi, H.K.;T.L. Adams;R.W. Stahlhut;S.I. Kim;J.H. Yun;B.K. Song;J.H. Kim;S.S. Hong;H.S. Lee
  7. U.S.Patent, 5,900,979 Method for Purifying Taxol from Taxus Biomass Hong, S.S.;B.K. Song;J.H. Kim;C.B. Lim;H.S. Lee;K.W. Kim;I.S. Kang;H.B. Park
  8. U.S.Patent, 5,281,727 Method of Using Ion Exchange Media to Increase Taxane Yields Carver, D.R.;T.R. Prout;C.T. Workman;D.L. Henderson;C.L. Hughes