• Journal of Embryo Transfer
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• v.16 no.1
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• pp.41-46
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• 2001

This study was carried out to evaluate the effect of culture methods on development of embryos with each developmental stage after heat shock in bovine oocytes. The results obtained were as fellows. 1. The culture method after heat shock on development of embryos was better drop-culture than co-culture. 2. The medium without amino acids were not effect of heat sock on development of embryos but it was in need of amino acid during formation of blastocyst.

• Korean Journal of Culture and Social Issue
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• v.21 no.1
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• pp.45-66
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• 2015

This study explored the moderating effects of social supports (family support, home friends support, foreign friends support) and cultural identity (home identity, foreign identity) on the relationships of reverse culture shock and subjective well-being. Participants were 157 returnees who left home-country prior to the age of 19 and resided in the foreign-country for more than three years. The results of hierarchical regression analyses on two-way interaction effect between reverse culture shock and each hypothesized moderator (e.g., family support, home friends support, foreign friends support, home identity, foreign identity) indicated that reverse cultural shock and subjective well-being was negatively related and their relationship was moderated only by family support. Specifically, the relationship between reverse culture shock and subjective well-being was weaker when the level of family support was higher. Subsequently, three-way interaction among reverse culture shock, one of the social support factors, and one of the cultural identity factors was investigated using hierarchical regression analyses. The results showed that the three-way interaction among reverse culture shock, family support, and home identity was significant. The slope difference tests yielded that the relationship between reverse culture shock and subjective well-being was stronger when both levels of family support and home identity were lower compared to when either level of family support or home identity was higher. These results imply that environmental factors such as family support and intrapsychic factor such as home identity might function as a buffer against the negative consequences of reverse culture shock experience.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.101-105
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• 2003

To eradicate several viruses such as PVX, PVY, and PLRV which often cause considerable damages to the growth and yields of potatoes, several stems including shoot tips were excised from the potato plants grown for 50 days and electric shock was treated. Shoot tips excised from electric-shocked stems were transferred into the medium supplemented with antiviral compound, ribavirin to examine the combinatorial effect. When treated only with 20 mg/L ribavirin, PVX concentration in the regenerated plant-lets was slowly decreased as repeating sub-culture and finally, it took 32 weeks to reach completely PVX-free stock. With an electric shock treatment (10 mA electric current), all the replicates became free from PVY. However, PLRV was not completely eradicated from 94P70-4 and 93P29-3 lines even by treating with 10 mA electric shock. In this case, both electric shock and antiviral compound treatments in axillary buds from the stem segment were successful in eradicating viral contamination.

• Animal cells and systems
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• v.13 no.3
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• pp.267-274
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• 2009

The dissociative culture technique of Aplysia neuron is one of the key methods that have been used for studies of cellular and molecular mechanisms of neuronal functioning. However, despite the advantages this method offers as an experimental model, its technical efficiency has had room for improvement. In this study, we examined certain putative factors that might affect the culture quality. The effects of neuronal damage induced by physical injuries, heat shock, and surface protein degradation were evaluated along with the correlation between the culture quality and animal behavior. As a result, we found that physical injury can be a critical factor that affects culture quality, whereas the heat shock and surface protein degradation had negligible effect on it. In addition, we discovered that siphon retraction time was not a good measurement for healthy neurons. Based on these findings, we suggest here an improved method in which the degree of physical injury is reduced by means of multiple protease treatment.

• Journal of Periodontal and Implant Science
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• v.34 no.1
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• pp.29-33
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• 2004

The present study was done to evaluate the environmental factors responsible for the expression of Porphyromonas gingivalis heat shock protein. The intensity of the heat shock protein gene expression was comparable to those seen by the heat shock ptreatment of the bacteria $(44^{\circ}C)$ when the bacteria was grown as a mixed culture or biofilm state at $37^{\circ}C$.

• The Journal of the Convergence on Culture Technology
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• v.4 no.3
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• pp.93-98
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• 2018

The article studies macroeconomic effects of the oil shock for Korea, which is a representative emerging economy of Asia and a small open economy. This article analyzed the macroeconomic effects of oil shocks in terms of demand and supply. In the case of Korea, oil price shocks different responds depending on factors of shock. Oil supply shock have led to a decline in industrial activity and interest rate, and oil specific demand shock have shown the greatest increase in interest rate relative to other oil price shocks. In addition, oil demand shock driven by economic activity showed that the comsumer price and the exchange rate are the largest compared to the oil shock caused by other factors. Therefore, policy makers will need to identify the source of the oil shock.

• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1454-1459
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• 2013

The changes in prodiginines productions caused by pH shock culture of Streptomyces coelicolor strains were estimated. In Streptomyces coelicolor M511, undecylprodiginine and streptorubin B productions increased 1.8-fold (37.22 mg/g) and 2.5-fold (18.61 mg/g), respectively, by pH shock (from 7.2 to 4.0). In contrast, this resulted in the significantly decreased prodigignines production in the redP deletion mutant SJM1; 3.7-fold for undecylprodiginine, 4.4-fold for streptorubin B, 5.2-fold for methylundecylprodiginine, and 6.4-fold for methyldodecylundecylprodiginine, respectively. RT-PCR analyses showed that, during pH shock, expression of redD, the transcription activator gene, was increased while the expression of fabH, the decarboxylative condensation enzyme gene in fatty acid biosynthesis, was decreased in both strains. The enhanced redD expression was in good accordance with the increased total prodiginines production of M511. However, for SJM1 mutant, the decrease of fabH expression occurred more strikingly, such that it became almost completely turned off during acidic pH shock culture. Therefore, a down-regulation of fabH was considered to be the cause of decreased amount of total prodiginines produced, although redD expression was high in SJM1 mutant.

• International Journal of Advanced Culture Technology
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• v.10 no.2
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• pp.187-193
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• 2022

We is configured the honk changing-status technique that is to meld the square-built blow-shock status of the gleam-differential perception level (BIAL) on the honk perception lineament. The perception level condition by the honk perception lineament system is constituted with the blow-shock system. As to experimentation a ductile-dot of the gleam ductile-dot, we are found of the honk value with ductile-dot by the blow upper shift. The concept of perception level is constituted the reference of gleam-differential level for changing-status signal by the honk shock lineament. Further symbolizing a square-built changing-status of the BIAL, of the average in terms of the blow-shock lineament, and the honk ductile-dot shock that was the honk value of the far changing-status of the Ho-PL-FA-θ_AVG with 15.41±8.63 units, that was the honk value of the convenient changing-status of the Ho-PL-CO-θ_AVG with 8.70±3.06 units, that was the honk value of the flank changing-status of the Ho-PL-HO-θ_AVG with 2.65±1.19 units, that was the honk value of the edge changing-status of the Ho-PL-VI-θ_AVG with 0.51±0.18 units. The blow shock will be to investigate at the square-built ability of the blow-shock lineament with ductile-dot by the honk perception level on the BIAL, that is denote the gleam-differential lineament by the perception level system. We will be possible to curb of a lineament by the differential signal and to employ the honk data of blow shock level by the blow perception system.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.658-662
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• 2008

Effects of pH shock on the secretion system of S. coelicolor A3(2) have been investigated at a transcriptional level by using DNA microarrays. Actinorhodin secretion was observed to be highly enhanced when an acidic-pH shock was applied to surface grown cultures of S. coelicolor A3(2). In this culture, a gene of actVA-orf1 encoding a putative efflux pump or transporter protein for actinorhodin was strongly upregulated. A major number of efflux pumps for other metabolites and a major number of secretion proteins for protein secretion were also observed to be upregulated with pH shock. The secretion of actinorhodin was observed to be remarkably enhanced in liquid culture as well.

• Journal of The Korean Society of Emergency Medicine
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• v.29 no.5
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• pp.474-484
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• 2018

Objective: The time to positivity (TTP) of blood culture reflects bacterial load and has been reported to be associated with outcome in bloodstream infections. This study was performed to evaluate the relationship between the TTP of blood culture and the mortality rates associated with sepsis and septic shock according to the site of infection. Methods: We performed a retrospective cohort study on patients with sepsis and septic shock. The rates of blood culture positivity and mortality as well as the relationship between the TTP and 28-day mortality rate were compared among patients with different sites of infection, such as the lungs, abdomen, urogenital tract, and other sites. Results: A total of 2,668 patients were included, and the overall mortality rate was 21.6%. The rates of blood culture positivity and mortality were different among the different infection sites. There was no relationship between the TTP and mortality rates of total, lung, and urogenital infections. Patients with abdominal infections showed a negative correlation between the TTP and 28-day mortality rate. In patients with abdominal infections, a TTP<20 hours was independently associated with 28-day mortality compared with patients with negative blood culture (hazard ratio, 1.73; 95% confidence interval, 1.16-2.58). However, there was no difference in mortality rates of patients with a $TTP{\geq}20$ hours and a negative blood culture. Conclusion: The shorter TTP in patients with abdominal infections in sepsis and septic shock was associated with a higher 28-day mortality rate.