• 한국수정란이식학회지
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• 제16권1호
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• pp.41-46
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• 2001

This study was carried out to evaluate the effect of culture methods on development of embryos with each developmental stage after heat shock in bovine oocytes. The results obtained were as fellows. 1. The culture method after heat shock on development of embryos was better drop-culture than co-culture. 2. The medium without amino acids were not effect of heat sock on development of embryos but it was in need of amino acid during formation of blastocyst.

• 한국심리학회지 : 문화 및 사회문제
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• 제21권1호
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• pp.45-66
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• 2015

본 연구에서는 해외유학경험 후 귀국한 성인들의 재문화충격 경험과 주관적 안녕감을 알아보고, 지지체계(가족지지, 모국친구지지, 외국친구지지)와 문화정체성(모국정체성, 외국정체성)이 재문화충격과 주관적 안녕감의 관계를 조절하는지를 살펴보았다. 연구참여자들은 19세 이전에 한국을 떠나 해외에서 3년 이상 거주를 하고 한국으로 되돌아온 157명의 귀국성인이었다. 위계적 회귀분석을 통해 재문화충격과 각각의 조절변인에 대해 이원상호작용을 분석한 결과, 재문화충격과 주관적 안녕감 간에는 부적인 관계가 있었으며, 가족지지의 조절효과만이 유의한 것으로 나타났다. 구체적으로, 재문화충격과 주관적 안녕감 간의 관계 강도는 가족지지가 높을 때 완화되는 것으로 나타났다. 다음으로 재문화충격, 사회적 지지 중 한 요인, 문화정체성 중 한 요인을 대상으로 삼원상호작용을 분석한 결과, 재문화충격, 가족지지, 모국정체성의 삼원상호작용이 유의하였다. 특히 재문화충격과 주관적 안녕감의 관계강도는 가족지지와 문화정체성 중 한가지만 낮을 때 보다 모두 다 낮을 때 더 높은 것으로 나타났다. 이러한 연구결과는 귀국성인들의 재문화충격과 주관적 안녕감의 관계가 조절가능하며 재문화적응과정에서의 가족지지(환경적 요인)와 모국정체성(개인내적 요인)의 중요성을 시사한다.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.101-105
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• 2003

To eradicate several viruses such as PVX, PVY, and PLRV which often cause considerable damages to the growth and yields of potatoes, several stems including shoot tips were excised from the potato plants grown for 50 days and electric shock was treated. Shoot tips excised from electric-shocked stems were transferred into the medium supplemented with antiviral compound, ribavirin to examine the combinatorial effect. When treated only with 20 mg/L ribavirin, PVX concentration in the regenerated plant-lets was slowly decreased as repeating sub-culture and finally, it took 32 weeks to reach completely PVX-free stock. With an electric shock treatment (10 mA electric current), all the replicates became free from PVY. However, PLRV was not completely eradicated from 94P70-4 and 93P29-3 lines even by treating with 10 mA electric shock. In this case, both electric shock and antiviral compound treatments in axillary buds from the stem segment were successful in eradicating viral contamination.

• Animal cells and systems
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• 제13권3호
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• pp.267-274
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• 2009

The dissociative culture technique of Aplysia neuron is one of the key methods that have been used for studies of cellular and molecular mechanisms of neuronal functioning. However, despite the advantages this method offers as an experimental model, its technical efficiency has had room for improvement. In this study, we examined certain putative factors that might affect the culture quality. The effects of neuronal damage induced by physical injuries, heat shock, and surface protein degradation were evaluated along with the correlation between the culture quality and animal behavior. As a result, we found that physical injury can be a critical factor that affects culture quality, whereas the heat shock and surface protein degradation had negligible effect on it. In addition, we discovered that siphon retraction time was not a good measurement for healthy neurons. Based on these findings, we suggest here an improved method in which the degree of physical injury is reduced by means of multiple protease treatment.

• Journal of Periodontal and Implant Science
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• 제34권1호
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• pp.29-33
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• 2004

The present study was done to evaluate the environmental factors responsible for the expression of Porphyromonas gingivalis heat shock protein. The intensity of the heat shock protein gene expression was comparable to those seen by the heat shock ptreatment of the bacteria $(44^{\circ}C)$ when the bacteria was grown as a mixed culture or biofilm state at $37^{\circ}C$.

• 문화기술의 융합
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• 제4권3호
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• pp.93-98
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• 2018

본 연구는 아시아의 대표적인 신흥경제국으로 원유수입국이며 소규모 개방경제인 한국을 대상으로 유가쇼크의 거시경제 효과를 충격반응함수를 추정하여 분석하였다는데 의의가 있다. 유가쇼크는 대부분 공급 측면의 요인에 의해 발생하는 것으로 알려져 왔으나, 본 연구는 유가쇼크를 수요와 공급 측면에서 세 가지로 구분하여 그 거시경제 효과를 분석하였다. 한국의 경우에 있어, 유가쇼크는 쇼크의 요인에 따라 상이한 반응을 보였다. 원유공급쇼크는 산업 활동과 금리를 하락시키며, 원유 자체 수요쇼크는 다른 요인에 의한 유가쇼크보다 상대적으로 금리를 가장 크게 상승시키는 것으로 나타났다. 그리고 경제호황에 의한 원유수요쇼크는 다른 요인에 의한 유가쇼크보다 물가와 환율을 상대적으로 가장 크게 상승시키는 것으로 나타났다. 따라서 정책당국은 유가쇼크의 요인에 따라 상이한 정책 대응을 할 필요가 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제23권10호
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• pp.1454-1459
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• 2013

The changes in prodiginines productions caused by pH shock culture of Streptomyces coelicolor strains were estimated. In Streptomyces coelicolor M511, undecylprodiginine and streptorubin B productions increased 1.8-fold (37.22 mg/g) and 2.5-fold (18.61 mg/g), respectively, by pH shock (from 7.2 to 4.0). In contrast, this resulted in the significantly decreased prodigignines production in the redP deletion mutant SJM1; 3.7-fold for undecylprodiginine, 4.4-fold for streptorubin B, 5.2-fold for methylundecylprodiginine, and 6.4-fold for methyldodecylundecylprodiginine, respectively. RT-PCR analyses showed that, during pH shock, expression of redD, the transcription activator gene, was increased while the expression of fabH, the decarboxylative condensation enzyme gene in fatty acid biosynthesis, was decreased in both strains. The enhanced redD expression was in good accordance with the increased total prodiginines production of M511. However, for SJM1 mutant, the decrease of fabH expression occurred more strikingly, such that it became almost completely turned off during acidic pH shock culture. Therefore, a down-regulation of fabH was considered to be the cause of decreased amount of total prodiginines produced, although redD expression was high in SJM1 mutant.

• International Journal of Advanced Culture Technology
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• 제10권2호
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• pp.187-193
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• 2022

We is configured the honk changing-status technique that is to meld the square-built blow-shock status of the gleam-differential perception level (BIAL) on the honk perception lineament. The perception level condition by the honk perception lineament system is constituted with the blow-shock system. As to experimentation a ductile-dot of the gleam ductile-dot, we are found of the honk value with ductile-dot by the blow upper shift. The concept of perception level is constituted the reference of gleam-differential level for changing-status signal by the honk shock lineament. Further symbolizing a square-built changing-status of the BIAL, of the average in terms of the blow-shock lineament, and the honk ductile-dot shock that was the honk value of the far changing-status of the Ho-PL-FA-θ_AVG with 15.41±8.63 units, that was the honk value of the convenient changing-status of the Ho-PL-CO-θ_AVG with 8.70±3.06 units, that was the honk value of the flank changing-status of the Ho-PL-HO-θ_AVG with 2.65±1.19 units, that was the honk value of the edge changing-status of the Ho-PL-VI-θ_AVG with 0.51±0.18 units. The blow shock will be to investigate at the square-built ability of the blow-shock lineament with ductile-dot by the honk perception level on the BIAL, that is denote the gleam-differential lineament by the perception level system. We will be possible to curb of a lineament by the differential signal and to employ the honk data of blow shock level by the blow perception system.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.658-662
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• 2008

Effects of pH shock on the secretion system of S. coelicolor A3(2) have been investigated at a transcriptional level by using DNA microarrays. Actinorhodin secretion was observed to be highly enhanced when an acidic-pH shock was applied to surface grown cultures of S. coelicolor A3(2). In this culture, a gene of actVA-orf1 encoding a putative efflux pump or transporter protein for actinorhodin was strongly upregulated. A major number of efflux pumps for other metabolites and a major number of secretion proteins for protein secretion were also observed to be upregulated with pH shock. The secretion of actinorhodin was observed to be remarkably enhanced in liquid culture as well.

• 대한응급의학회지
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• 제29권5호
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• pp.474-484
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• 2018

Objective: The time to positivity (TTP) of blood culture reflects bacterial load and has been reported to be associated with outcome in bloodstream infections. This study was performed to evaluate the relationship between the TTP of blood culture and the mortality rates associated with sepsis and septic shock according to the site of infection. Methods: We performed a retrospective cohort study on patients with sepsis and septic shock. The rates of blood culture positivity and mortality as well as the relationship between the TTP and 28-day mortality rate were compared among patients with different sites of infection, such as the lungs, abdomen, urogenital tract, and other sites. Results: A total of 2,668 patients were included, and the overall mortality rate was 21.6%. The rates of blood culture positivity and mortality were different among the different infection sites. There was no relationship between the TTP and mortality rates of total, lung, and urogenital infections. Patients with abdominal infections showed a negative correlation between the TTP and 28-day mortality rate. In patients with abdominal infections, a TTP<20 hours was independently associated with 28-day mortality compared with patients with negative blood culture (hazard ratio, 1.73; 95% confidence interval, 1.16-2.58). However, there was no difference in mortality rates of patients with a $TTP{\geq}20$ hours and a negative blood culture. Conclusion: The shorter TTP in patients with abdominal infections in sepsis and septic shock was associated with a higher 28-day mortality rate.