• 한국어병학회지
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• 제33권1호
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• pp.63-69
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• 2020

Fish culture is constantly threatened by various infectious diseases which are largely transmitted by water. Plasma technology is being used to sterilize polluted water in many industries. In this study, two bacterial pathogens Aeromonas salmonicida and Streptococcus iniae, and a virus (viral hemorrhagic septicemia virus, VHSV) were subjected to plasma water that was produced by a corona discharge system. Growth of A. salmonicida was greatly inhibited from 10^5.61 CFU/ml in positive control to 10^3.51 CFU/ml in treated group by only 60 sec contact with plasma water. Similarly, S. iniae was inhibited from 10^5.85 CFU/ml to 10^3.40 CFU/ml. VHSV titer also decreased from 10^4.1 TCID₅₀/ml to 10^1.45 TCID₅₀/ml by the same treatment. Activation of water by the plasma was confirmed by the existence of ozone in the plasma water. These results suggest that plasma water could efficiently disinfect fish pathogens, possibly by the action of reactive oxygen species contained in the plasma water.

• 디자인학연구
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• 제18권2호
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• pp.345-354
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• 2005

20세기부터 교통과 산업이 발달함에 따라 국제적인 교류가 더욱 활발해지면서 각국에서 사용하는 픽토그램의 국제표준에 대한 중요성이 강조되고 있다. 하지만 근원적으로 서로 다른 배경과 문화를 가진 국가간의 합의를 이루어낸다는 것은 결코 쉬운 일이 아니기 때문에 국제표준화기구에서는 1947년 설립 이래 국제표준(ISO)을 제정하는 기준과 절차에 대한 논의가 끊임없이 되풀이되고 있는 실정이다. 현재 사용하고 있는 ISO 9186이라는 그래픽 심볼의 평가방법은 그 절차가 까다롭고 비용이 많이 드는데 비해 기호를 읽고 해석하는 경험세계의 본질적인 변수를 반영하고 있지 못하기 때문에 점점 회원국의 호응을 잃어가고 있다. 본 연구는 그래픽 기호와 지각메카니즘에 대하여 그 이론적 배경을 살펴보고 네트 문화의 시대를 살고 있는 우리에게 과연 문화적 차이는 존재하는지 디지털 미학의 관점에서 현실세계의 사물이 갖고 있는 리얼리티는 어떻게 해석되는지를 고찰한다. 3장에서는 국제표준화 절차와 기준의 문제점을 분석하고 지각메커니즘에 의한 그래픽기호의 전달과정이론에 바탕을 둔 픽토그램의 국제표준화는 어떻게 이루어져야하는지 또한 반드시 고려되어야할 사항은 무엇인지에 대해서 연구한다.

• 한국생활과학회지
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• 제16권3호
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• pp.615-622
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• 2007

Jeonju is well-known as a native place of traditional Hanji from ancient times because of it's proper weather, soil, and sunlights. Traditional Hanji is selected one of the Traditional Cultural Contents Industries of Jeonju nationally, and can produce synergy effects on gaining competition if we develop traditional Hanji industry into high-technology by relating cultural contents. The purposes of this study was to identify consumer attitudes and behaviors toward traditional Hanji products. The results of this study were as followed: 1. Representative products of Jeonju City were a rice with vegetables, a traditional Hanji, and a Korean-style house, and these results showed a consistancy with Han-Brand products promoted by Ministry of Culture and Tourism. 2. Many consumers experienced of a box, a copy paper, a book or notebook made of Hanji, but less experienced of fashion accessary products. Consumers had higher interests in wall or window paper and copy paper, but had lower interests in fashion accessary products such as necktie, shawl, and folding fan. More consumers intended to use wall or window paper and copy paper, but intended fashion accessary products less. Many kinds of Hanji products were produced and displayed on a Hanji Crafts House, but only few consumers had experiences. 3. Younger generations had higher interests and experiences of Hanji products, and unmarried officiers or full-time house wives had experiences Hanji crafts more. 4. Consumers were interested in daily necessaries or interior products made of Hanji.

• Journal of Microbiology and Biotechnology
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• 제21권12호
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• pp.1270-1279
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• 2011

Bealmijang is a short-term aged paste made from meju, which is a brick of fermented soybeans and other ingredients. Different types of bealmijang are available depending on the geographic region or ingredients used. However, no study has clarified the microbial diversity of these types. We identified 17 and 14 fungal species from black soybean meju (BSM) and buckwheat meju (BWM), respectively, on the basis of morphology, culture characteristics, and internal transcribed spacer and ${\beta}$-tubulin gene sequencing. In both meju, Aspergillus oryzae, Rhizopus oryzae, Penicillium polonicum, P. steckii, Cladosporium tenuissimum, C. cladosporioides, C. uredinicola, and yeast species Pichia burtonii were commonly found. Moreover, A. flavus, A. niger, P. crustosum, P. citrinum, Eurotium niveoglaucum, Absidia corymbifera, Setomelanomma holmii, Cladosporium spp. and unclassified species were identified from BSM. A. clavatus, Mucor circinelloides, M. racemosus, P. brevicompactum, Davidiella tassiana, and Cladosporium spp. were isolated from BWM. Fast growing Zygomycetous fungi is considered important for the early stage of meju fermentation, and A. oryae and A. niger might play a pivotal role in meju fermentation owing to their excellent enzyme productive activities. It is supposed that Penicillium sp. and Pichia burtonii could contribute to the flavor of the final food products. Identification of this fungal diversity will be useful for understanding the microbiota that participate in meju fermentation, and these fungal isolates can be utilized in the fermented foods and biotechnology industries.

• 한국콘텐츠학회논문지
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• 제9권8호
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• pp.166-177
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• 2009

의학 기술의 발달과 저 출산 현상으로 인구의 고령화가 진행되어감에 따라, 실버세대를 위한 다양한 문화와 산업이 대두되고 있다. 실버 세대는 상대적으로 많은 여가시간을 보내기 위해 다양한 사회 활동과 문화 콘텐츠를 경험하길 원하지만, 노령화에 따른 신체적 심리적 정신적 기능 저하로 인해 제약이 따르게 된다. 본 논문에서는 실버 세대들이 사회활동에서 가장 필요로 하는 이동성 확보에 도움이 되는 하체 단련과 체력 증진 기능을 갖춘 체감형 자전거 게임 콘텐츠를 제안한다. 제안된 게임 콘텐츠는 실버세대에게 익숙한 자전거라는 기구를 이용하여 노인들에게 적합한 게임 구성과 디자인을 통해 게임에 대한 흥미를 유발시킬 수 있다. 노인들은 게임의 몰입감을 증대시키기 위해 개발된 체감형 인터페이스와 실시간 3D 그래픽스 디스플레이를 통해 놀이와 운동을 동시에 즐기며 여가시간을 활용할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2082-2089
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• 2015

Avian beta-defensin 9 (AvBD9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. Owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, AvBD9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. Using the preferred codon of Pichia pastoris, the mature AvBD9 peptide was designed and synthesized, based on the sequence from GenBank. The P. pastoris constitutive expression vector pGHKα was used to construct a pGHKα-AvBD9 recombinant plasmid. Restriction enzyme digestion was performed using SacI and BglII to remove the ampicillin resistance gene, and the plasmid was electrotransformed into P. pastoris GS115. High-expression strains with G418 resistance were screened, and the culture supernatant was analyzed by Tricine-SDS-PAGE and western blot assay to identify target bands of about 6 kDa. A concentrate of the supernatant containing AvBD9 was used for determination of antimicrobial activity. The supernatant concentrate was effective against Escherichia coli, Salmonella paratyphi, Salmonella pullorum, Pseudomonas aeruginosa, Enterococcus faecalis, and Enterobacter cloacae. The fermentation product of P. pastoris carrying the recombinant AvBD9 plasmid was adjusted to 1.0 × 10⁸ CFU/ml and added to the drinking water of white feather broilers at different concentrations. The daily average weight gain and immune organ indices in broilers older than 7 days were significantly improved by the AvBD9 treatment.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1221-1228
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• 2013

Two lipase genes (bpl1 and bpl3) from Antarctic Bacillus pumilus strains were expressed in Bacillus subtilis. Both recombinant lipases BPL1 and BPL2 were secreted to the culture medium and their activities reached 3.5 U/ml and 5.0 U/ml, respectively. Their molecular masses apparent using SDS-PAGE were 23 kDa for BPL1 and 19 kDa for BPL3. Both lipases were purified to homogeneity using ammonium sulfate precipitation and HiTrap SP FF column and Superose 12 column chromatographies. The final specific activities were estimated to be 328 U/mg for BPL1 and 310 U/mg for BPL3. Both lipases displayed an optimum temperature of $35^{\circ}C$, similar to other mesophilic enzymes. However, they maintained as much as 70% and 80% of the maximum activities at $10^{\circ}C$. Accordingly, their calculated activation energy at a temperature range of $10-35^{\circ}C$ was 5.32 kcal/mol for BPL1 and 4.26 kcal/mol for BPL3, typical of cold-adapted enzymes. The optimum pH of BPL1 and BPL3 was 8.5 and 8.0, respectively, and they were quite stable at pH 7.0-11.0, showing their strong alkaline tolerance. Both lipases had a preference toward medium chain length ($C_6-C_{10}$) fatty acid substrates. These results indicate the potential for the two Antarctic B. pumilus lipases as catalysts in bioorganic synthesis, food, and detergent industries.

• 한국콘텐츠학회논문지
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• 제16권11호
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• pp.358-368
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• 2016

김치산업은 우리나라의 문화에 대한 척도로서 국민들의 자긍심을 높여주는 산업일 뿐만 아니라 국경제발전과 국민의 삶의 질 향상에 기여하고 있어 반드시 육성해야 하는 전략산업 중의 하나이다. 그러나 김치산업의 중요성과 가치에 대한 국가적인 공감대의 형성 부족으로 큰 어려움을 겪고 있다. 따라서 본 연구의 목적은 한국은행이 2015년에 발표한 2013년 산업연관표를 이용하여 김치산업이 국민경제에 얼마만큼 기여하는지를 비교분석하는데 있다. 이를 위해 본 논문에서는 한국은행의 2013년 산업연관표흫를 활용하여 김치산업연관표 만들어 분석해야 한다. 분석결과 김치산업의 총샌산유발액은 564,254십억원, 생산유발계수는 1.8418(열), 1.1760(행), 감응도계수는 0.6136 영향력계수는 0.9611 소득유발계수 0.1876 생산세유발계수는 0.0084 그리고 노동유발계수는 0.003 등으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1540-1548
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• 2012

Manganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAE-cellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and $35^{\circ}C$, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0, and at temperatures of up to $35^{\circ}C$ at a pH of 4.5 for 1h. The $K_m$ and $V_{max}$ values for the substrate phenol red were found to be $8{\mu}m$ and 111.14 U/mg of protein, respectively. The MnP also oxidized other substrates such as guaiacol, DMP, and veratryl alcohol. Sodium azide, EDTA, SDS, $Cu^{2+}$, and $Fe^{2+}$, at 1-5 mM, strongly inhibited enzyme activity, whereas $Ca^{2+}$ and $Zn^{2+}$ increased enzyme activity. The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.

• Mycobiology
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• 제44권3호
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• pp.155-161
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• 2016

The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation. This study was performed to classify the most efficient strains of Aspergillus section Flavi isolated from various types of traditional wheat nuruk, based on a polyphasic approach involving molecular and biochemical evaluation. A total of 69 strains were isolated based on colony morphology and identified as Aspergillus oryzae/flavus based on internal transcribed spacer and calmodulin gene sequencing. Interestingly, none were toxigenic based on PCR amplification of intergenic regions of the aflatoxin cluster genes norB-cypA and the absence of aflatoxin in the culture supernatants by thin-layer chromatography analysis. Saccharification capability of the isolates, assessed through ${\alpha}-amylase$ and glucoamylase activities, revealed that two isolates, TNA24 and TNA15, showed the highest levels of activity. Although the degrees of variation in ${\alpha}-amylase$ and glucoamylase activities among the isolates were higher, there were only slight differences in acid protease activity among the isolates with two, TNA28 and TNA36, showing the highest activities. Furthermore, statistical analyses showed that ${\alpha}-amylase$ activity was positively correlated with glucoamylase activity (p < 0.001), and therefore screening for either was sufficient to predict the saccharifying capacity of the Aspergillus strain.