• Journal of Mushroom
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• v.15 no.2
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• pp.73-77
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• 2017

In Korea, oyster mushroom is one of the commonly cultivated mushrooms. In 2013, the cultivation areas and products of oyster mushrooms were 60,039 M/T and 201 ha, respectively. Many species of oyster mushrooms are cultivated in various countries. These include Pleurotus ostreatus, P. florida, P. sajor-caju, P. eryngii, P. citrinopileatus, P. salmoneostramineus and P. cystidiosus. P. citrinopileatus is a yellow oyster mushroom famous for its health benefits such as anti-cancer and anti-oxidant effects. Therefore, a cultivar P. citrinopileatus 'Jangdari' was developed to improve yield and the ability to grow well at lower temperatures. Two parent strains 'Gumbit (KMCC02150)' and 'KMCC02145' were selected based on their morphological characteristics. 'Jangdari' was developed by the method of Mon-Mon crossing between monokaryons derived from 'Gumbit' and 'KMCC02145', and finally selected through continuous cultivation tests. The optimum temperature for mycelial growth was $30^{\circ}C$. The cultivar could grow well at high temperatures, especially $16{\sim}24^{\circ}C$. Fruiting body production per bottle (850 mL) was about 90.0 g. Stipe length and thickness of 'Jangdari' were similar to those of 'Gumbit'. 'Jangdari' was more resistant to low temperature than 'Gumbit', and thus it could be cultivated with oyster mushrooms (P. ostreatus). In addition, while cultivating 'Jangdari', it is not required to scrape out the upper side of bottle's sawdust medium; hence, its cultivation is expected to save energy and time.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.211-222
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• 1995

This experiments were carried out to investigate the growth responses of spinach to temperature controlling by shading treatments at different times during the summer, and the effects of environmental parameters on the growth and yield were evaluated. The fourth cultivation was showed maximum temperature at air temperature of 33.1$^{\circ}C$ and soil temperature of 28.6$^{\circ}C$, followed by third, second, fifth and first cultivation. Among the tested temperature decreasing by shading, shading with silver - coated PE film strips was the most effective in lowering greenhouse air temperatures by showing 1.1$^{\circ}C$ decrease as compared to the outside air temperature and 3.1$^{\circ}C$ decrease as compared to the air temperatures inside the non-shaded PE house and 2.3$^{\circ}C$ decrease as compared to the air temperaturese shading with black- coated PE film strips, the soil temperature was showing 2$^{\circ}C$ decrease in both shading treatments than non-shaded PE and outdoor. In first cultivation, difficult cultivated as compared to others period owing to bolting of spinach. This first period was cultivated used two domestic cultivars and six imported cultivars, significantly better growth and higher yield of spinach were recorded in glory, manchuparuk and sunlighr cultivars. The effectiveness of shading on spinach, as expressed by the crop yield increase, was the highest in the planting from July 1 to August 24.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.4
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• pp.276-283
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• 2023

Garlic (Allium sativum L.) is one of the most important vegetables used in various foods in Korea and many countries. The growth of garlic is influenced by various abiotic factors such as cultivation temperature, humidity, minimum temperature duration, and photoperiod. This study investigated the effects of increasing temperatures on the plant growth of the northern- ecotype garlic 'Uiseong' in a temperature gradient tunnel. As a result, temperature increase led to decreases in the bulb diameter, weight, and clove pieces of garlic. The rise of cultivation temperature increased the occurrence rate of incomplete bolting in the Northern-ecotype garlic 'Uiseong', resulting in decreases in productivity and a decrease in the yield of marketable garlic, indicating that temperature increases affect the development of garlic bulb formation. The findings of this study are expected to contribute as foundational data for understanding the growth responses of the northern-ecotype 'Uiseong' to increasing cultivation temperatures. The results of this study can be used to develop designing garlic growth models. In addition, the results of this study can improve understanding the interaction between increased temperature and garlic growth.

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.19-25
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• 2001

Induction of somatic embryogenesis from Brazilian eggplant variety F-100 was studied in response to four auxin types. NAA, at the optimal concentration of 54 $\mu\textrm{m}$, was the only one that resulted in the induction of somatic embryos in either leaf and cotyledon explant and, at murk lower intensity and frequency, in hypocotyl and epicotyl explants. The optimal temperatures for embryo induction were 28 and 35$^{\circ}C$ for cotyledon and leaf explants. Incubation at 22$^{\circ}C$ caused a significant reduction both in the frequency and intensity of induction. This system was used to study the effects of position and orientation of the tissue on the culture medium as well as of antibiotics and explant co-cultivation with Agrobacterium on the efficiency of somatic embryo induction. The intensity of embryo induction was greater in the midsections of cotyledons relative to apical and basal regions, when the abaxial surface was in contact with the culture medium. The presence of antibiotics resulted in approximately 40-60% reduction of embryo induction relative to control explants, which originated 335$\pm$26.6 embryos. Co-cultivation with Agrobacterium before treatment with antibiotics caused a more drastic reduction (80-99%). Ampicilin treatment after cocultivalion with Agrobacterium caused the least inhibitory effect, allowing the production of 60 embryos/explant.

• Korean Journal of Microbiology
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• v.47 no.3
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• pp.179-187
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• 2011

This review describes the characteristics of various unculturable soil bacteria, successfully-cultivating examples of those bacteria, and the diverse factors to be considered for successful cultivation. Most importantly, the selection of proper media is very important because unculturable bacteria demand different types of nutrients at various concentrations of substrates, nitrogens and phosphorus. To develop a new medium to successfully culture unculturable bacteria from soil, molecular ecological studies should be combined together. The inoculum size on a plate is also important: less than 50 bacterial cells are recommended to be plated on a single culture plate. The environmental factors such as pH and salt concentration of the medium need to be adjusted as similar as possible to mimic the original soil environments, and the trial of the various temperatures and extended period of cultivation are better. Since one cannot simply tell about which one was unculturable among a great number of colonies grown on a newly developed medium, some suitable detection methods and fast identification methods are required. Many soil bacteria live with cooperation one another in their communities, so that enrichment such as coculture of using other bacterial metabolites and subsequent pure cultures can also guarantee successful cultivation of the previously uncultured bacteria in soil. Here, this review will discuss for the future perspectives to culture the unculturable soil bacteria.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.104-111
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• 2015

In this study, a new strain of Lentinula edodes (Shiitake) Poongnyunko was bred from dikaryotic KFRI 490 and monokaryotic KFRI 536 by Di-mon crossing method. In this study, the productivity was conducted through bed-log and sawdust substrate cultivation. The Poongnyunko showed significant intensive production from summer to autumn. Optimum temperatures of fruit-body formation were $17{\sim}24^{\circ}C$. The total amount of fruit-body production during 4 years by bed-log cultivation was $22kg/m^3$, and total yield of fruit-body until the 3rd flush was 648 g/ 2 kg. In case of sawdust substrate cultivation, the mycelial incubation period was 110~130 days. The suitable temperature and relative humidity were $18{\pm}1^{\circ}C$ and $90{\pm}5%$ respectively.

• Journal of Digital Convergence
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• v.18 no.4
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• pp.243-250
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• 2020

Systematic farming can be planned and managed if long-term agricultural weather information of the plantation is available. Because the greatest risk factor for crop cultivation is the weather. In this study, a method for long-term predicting of agricultural weather using the GloSea5 and machine learning is presented for the cultivation of Chinese cabbage. The GloSea5 is a long-term weather forecast that is available up to 240 days. The deep neural networks and the spatial randomforest were considered as the method of machine learning. The longterm prediction performance of the deep neural networks was slightly better than the spatial randomforest in the sense of root mean squared error and mean absolute error. However, the spatial randomforest has the advantage of predicting temperatures with a global model, which reduces the computation time.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.9
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• pp.630-636
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• 2012

The purpose of this study was to determine optimum wavelength, light intensity, aeration rate, and temperature for the cultivation of Dunaliella salina illuminated by various types of light emitting diode. Growth rates of Dunaliella salina were faster at higher temperature than the growth rate at lower temperature. Among the culturing temperatures, $22^{\circ}C$ was the optimum temperature for the growth of Dunaliella salina. White LED was the most efficient light source and lower light intensity (3,000 Lux) resulted in better biomass production (1.30 g/L). The value of aeration varied between 0 and 2.4 vvm at the illumination of 3,000 Lux of white light emitting diode. Highest specific growth rate of $1.12day^{-1}$ was obtained at no-aeration and lower specific growth rates were obtained for other aeration tests, which indicated that aeration could be harmful for the cultivation of Dunaliella salina.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2001.10a
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• pp.256-260
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• 2001

This study was initially performed to investigate current red-pepper drying methods commonly being adopted on red-pepper cultivation farm area. Based on the informations obtained from the field survey, an experiment of red-pepper drying was carried out to verify the actual drying potential of plastic covered solar house similar to the conventional pipe frame greenhouses covered with one or two layer of plastic film. Some results obtained from field survey and drying experiment for red-pepper are summarized as follows; 1. Various patterns of red-pepper drying process were found; 1) complete natural drying with red-pepper exposed in outdoor air, 2) hot air drying by dry chamber only, 3) combination drying by hot air dryer together with plastic covered passive solar house, 4) drying with plastic covered solar house unit. 2. The average air temperatures of outdoor and solar house during drying experiment period were $26.9-30.8\;and\;28.6-33.8^{\circ}C$, respectively, and the maximum air temperatures of those two were $34.2-36.4\;and\;39.8\;-52.3^{\circ}C$, respectively. Horizontal solar intensity during experiment period was $18.49-23.96\;MJ/m^{2}$, and relative humidity of outdoor and experimental solar house were 56 - 66% and 64 - 70%, respectively. 3. The weight of red-pepper during drying experiment period was decreased almost linearly from initial moisture content of 85% to final moisture content of 14%.

• KSBB Journal
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• v.18 no.5
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• pp.418-423
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• 2003

A laboratory jar fermenter was modified to measure the duration for cooling water supply and the temperatures of the coolant at the inlet and outlet of water jacket. Successful operation of temperature control and on-line measurement was achieved by adjusting optimum parameters of the Proportion-Integral-Derivative temperature controller. The variables measured on-line were used to estimate cooling rates from empirical equations comprised of the time period of cooling water supply and the temperatures of coolant. The measured cooling rate showed a good correlation to the specific growth rate during batch cultivation of E. coli. Cooling rate was measured and applied to programmed cell growth in a fed-batch cultivations. Three fed-batch cultivations were demonstrated by feeding substrate to follow the programmed cooling rates increasing exponentially.