• 산업기술연구
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• 제21권A호
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• pp.337-341
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• 2001

This study was carried out to screen the effective polymeric additives preventing wall growth during mycelial submerged cultivation of Ganoderma. lucidum. Effects of additives on mycelial growth and exo-polysaccharide (EPS) production in flask culture and jar fermenter system under 3 different pH processes were investigated, and changes of mycelial morphology were also examined. From flask culture of G. lucidum with additives of different concentrations, 0.1%(w/v) polyacrylic acid was effective for EPS production. As the polyacrylic acid of 0.1%(w/v) was added in medium, wall growth of G. lucidum mycelium grown in jar fermenter system could be protected. The addition of 0.1%(w/v) polyacrylic acid to medium was also improved the mycelial growth and EPS production in the later of submerged culture G. lucidum and no changes of mycelial morphology were observed.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.111-115
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• 1991

The green alga, Dunaliella salina under fed-batch cultivation produced 51.12 mg of hydrocarbon per liter with maintaining 0.313 (g dry wt/l). About 20% of hydrocarbon production yield based on dry biomass was obtained from both batch and fed-batch processes. Optimum culture conditions of light intensity, pH and salt concentration were obtained as 0.0080 (kJ/$cm^2$/h), 8.0 and 1.4 (g of NaCl/l), respectively by response surface analysis. The production of hydrocarbons in D. salina was closely correlated to cell growth. Fed-batch cultivation produced more hydrocarbons and maintained better cell growth than a batch process.

• 한국산림과학회지
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• 제107권3호
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• pp.316-323
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• 2018

본 연구는 고사리, 곰취, 산마늘 등 주요 산채류에 대한 재배공정을 조사하여 소득분석을 실시함으로써 향후 임산물에 대한 손실보상 기준 및 임업경영진단 등에 필요한 기초자료를 제공하기 위하여 실시하였다. 조사결과 품목별 재배형태는 크게 노지재배와 산지재배로 구분되었다. 품목별 단위면적당($3.3m^2$) 연간 평균소득은 고사리 노지재배는 6.5천원, 고사리 산지재배는 3.2천원, 곰취 노지재배는 20.4천원, 산마늘 노지재배는 20.9천원, 산마늘 산지재배는 7.3천원 등으로 나타났다. 특히, 최근 소비자 수요가 크게 증가하고 있는 산마늘과 곰취의 노지재배 단위면적당($3.3m^2$) 연간 평균소득은 고사리에 비해서 매우 높은 것으로 분석되었다. 이러한 원인은 고사리 재배 시 경영비(토지임차료, 고용노동비)의 투입비중이 타 품목에 비해 매우 높기 때문이다. 연간 평균소득이 높은 곰취와 산마늘의 경우 종묘 및 종근 구입 등 초기 투자비용이 경영비의 평균 40% 이상을 차지하고 있어 향후 농산촌지역의 소득원 및 재배 활성화를 위해서는 이에 대한 정부의 적절한 지원이 필요할 것으로 판단된다.

• 한국자원식물학회지
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• 제26권1호
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• pp.52-59
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• 2013

본 연구는 실내 조경 및 분화용 소재로 활용가치가 높은 물가양털이끼(Brachythecium rivulare)와 쥐꼬리이끼(Myuroclada maximowiczii)의 적정 재배법을 개발하기 위하여 수행하였다. 원예상토 위에 천을 덮어 재배하는 것이 재배용기에 천이나 토양만 사용하는 방법에 비해 이끼 2종의 생육이 왕성하고, 수확이 편리하였다. 재배 토양은 바크나 피트모스 보다는 원예상토를 사용하였을 때 생육 및 수확의 편이성이 우수하였다. 식재할 때는 이끼를 분주하는 것 보다 믹서기로 분쇄하여 재배용기 위에 산포하는 방법이 이끼 2종의 배우체 발생과 수확 편이도 개선에 효과적이었다. 또한 재배용기($27{\times}17{\times}3cm$)에 물가양털이끼는 2.0 g, 쥐꼬리이끼는 4.0 g을 식재하였을 때, 생육이 가장 왕성하였고 수확이 편리하였다. 양액(N:P:K=20:20:20)을 농도별로 처리하였을 때 물가양털이끼는 무처리구에 비해 전반적으로 생육이 억제되었고, 고농도로 갈수록 생체중의 증가량이 감소하였다. 그러나 쥐꼬리이끼는 $0.25g{\cdot}L^{-1}$ 처리구에서 생체중의 증가량이 가장 많았다. 따라서 물가양털이끼와 쥐꼬리이끼는 원예상토 위에 천을 덮어서 만든 재배용기에 각 2.0, 4.0 g의 이끼를 분쇄하여 산포한 다음 충분한 수분을 공급하고, 쥐꼬리이끼는 재배기간 동안 저농도의 양액을 처리해주는 것이 적합한 재배법으로 생각된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.173-176
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• 2000

A neural network based controller (NN controller) was studied for the control of ammonia concentrations in biological processes. An ammonia FIA has been employed to on-line monitor the concentrations of ammonia in a bioreactor. The optimal neural network structure was investigated by computer simulation and found to be a 3(inputlayer)-2(hidden layer)-1(output layer). The NN controller had advantage over the PID controller, even though the former is more time consuming. The 3-2-1 NN controller has been used to control the ammonia concentrations in a simulated bioprocess and also in a real cultivation process of yeast, and its performance were investigated.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.270-273
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• 2001

The fluorescence sensor is utilized to monitor the complex fluorescence patterns of intra- and extracellular components in cultivation processes. Especially biogenic fluorophores such as proteins and peptides (tryptophan, phenylalanine), coenzymes (FAD, NAD(P)H) and vitamins (riboflavin, pyridoxine) within cells are detected by a fluorescence sensor. In this work a 2-dimensional fluorescence sensor has been used to monitor a production process of itaconic acid by Aspergillus terreus and the on-line monitored spectra data can be con-elated to off-line data measured by a few methods.

• Molecules and Cells
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• 제40권2호
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• pp.90-99
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• 2017

Caenorhabditis elegans is an important model organism with many useful features, including rapid development and aging, easy cultivation, and genetic tractability. Survival assays using C. elegans are powerful methods for studying physiological processes. In this review, we describe diverse types of C. elegans survival assays and discuss the aims, uses, and advantages of specific assays. C. elegans survival assays have played key roles in identifying novel genetic factors that regulate many aspects of animal physiology, such as aging and lifespan, stress response, and immunity against pathogens. Because many genetic factors discovered using C. elegans are evolutionarily conserved, survival assays can provide insights into mechanisms underlying physiological processes in mammals, including humans.

• 공업화학
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• 제33권2호
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• pp.145-150
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• 2022

Microalgae are attracting attention as a resource for the production of biofuels, food nutrients, biochemicals, and bioplastics. Among a wide range of sources of the biomass, microalgae have been highlighted due to relatively easy cultivation, ability to eliminate carbon dioxide, and low culturing cost. Despite the great potential of microalgal biomass as a biological material, the complexity and relatively expensive downstream processes have inhibited the commercial use of microalgae. In this study, we reviewed recent techniques for microalgal drying for the production of microalgal based products. As drying processes comprise the largest portion of microalgae processing cost, an efficient drying technique is key to the utilization of microalgal biomass.

• KSBB Journal
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• 제24권2호
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• pp.140-148
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• 2009

The microbial biosurfactants can be substituted to the chemical detergents in some industrial processes. In this study we developed a biotechnological processes for the biosurfactants with microorganisms. The biosurfactants have a lot of advantages in comparision with the chemical surfactants. They are proenvironmental even during and after industrial use. But there are not so many kinds of biosurfactants. The production cost and the end price is much higher than the chemical surfactants. But nowdays there are many kinds of microorganisms, which can produce the surfactants in large quantity and fast. We tried to develop a production process for the large scale with some microorganisms. At first Candida bombicola KCTC 7145, Sphingomonas chungbukensis KCTC 2955 and Sphingomonas yanoikuyae KCTC 2818 are cultivated and studied. For the large scale production process we used molasses as a complex medium and tried to optimize the process. Molasses contains 17 to 25% of water, 45 to 50% of sugar and 25% of carbohydrate, it can be fully used as a substrate. The microorganisms have been cultivated in the diluted media with molasses 2, 5, 8 and 10%, respectively, The optimal conditions for the cultivation and the production process have been studied. For the study the optical density, glucose concentration and the surface tension were measured. Candida bombicola KCTC 7145 and the 5% molasses media was selected as an optimal condition for the production process of a biosurfactant. During cultivation of Candida bombicola KCTC 7145 in the 5% molasses medium kerosene and corn oil were added for promoting the biosurfactants.

• 한국지역지리학회지
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• 제4권2호
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• pp.31-47
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• 1998

본 연구의 연구목적은 남강 하류 범람원의 토지이용 변화와 작물재배 체계를 분석함으로써 남강 하류 범람원 지역의 지역성을 구명하는 데 있다. 본 연구에서는 남강 하류 범람원 상에 발달한 큰들, 구해들, 장지들, 백산들, 하기들, 장포들 등 비교적 큰 평야들을 중점적인 조사지역으로 설정하였다. 대규모 제방이 축조되기 이전에 남강 하류 범람원의 대부분은 습지(새밭)로 방치되어 있었고, 자연제방과 구릉성 산지의 말단부만이 농경지로 이용되었다. 대규모의 경지정리와 더불어 남강 하류 범람원이 비옥한 농경지로 바뀌게 되는 것은 남강댐과 남강방수로가 각각 1969년과 1970년에 완공된 이후의 일이었다. 남강 하류 범람원 상에 조성된 농경지에서 여름부터 가을까지는 주로 벼농사를 하고 있고, 가을걷이가 끝난 이후인 늦가을부터 초여름까지는 주로 시설채소농업을 하고 있다. 근래 비닐하우스를 이용한 시설채소농업의 비중이 증대되었는데, 1980년대부터는 시설채소농업으로 수박 재배가 가장 중요시되었다. 그리하여 오늘날 남강 하류 범람원에서 벼농사는 오히려 부수적인 농업 활동이 되었다.