• KSBB Journal
• /
• 제28권1호
• /
• pp.24-30
• /
• 2013

Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a $MABOOMS^{TM}$ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru ${(dpp)_3}^{2+}$ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.

• 한국농공학회논문집
• /
• 제51권5호
• /
• pp.95-100
• /
• 2009

The water purification systems have been hardly used for agricultural purpose due to their complicated compositions and high costs for farmers, while only simple filtrations have been applied to irrigation systems in order to prevent the system from clogging problems. This study therefore developed a clean water supplying system, the Filter-Disinfection-Adsorption (FDA) system, especially for greenhouse cultivation of where low quality of water is available. This system has also been produced for providing convenience water to farmers in the areas of no water supply service systems for the purpose of washing their bodies or agricultural machineries after farm work. The FDA system consists of three stages of purification processes with an integral module, including disk and teflon filtrations and Ultraviolet (UV) sterilization processes. Indoor experiments were undertaken with a trial product of the FDA system to test its performance. The operation test of the process was performed as well as the condition check of each item including UV module, filters, control panel, pump, valves, etc. The results shows good performance of each test with no critical problems. The initial and maintenance costs were also analysed with other purification systems. From the comparisons, the FDA system found to be very economical and easy to use.

• Journal of Microbiology and Biotechnology
• /
• 제3권1호
• /
• pp.46-50
• /
• 1993

In 12:12 hour light/dark cycle cultivation of D. salina 1650, maximum specific growth rate of 0.59 (l/day) and 0.35 (g-crude hydrocarbons/l/day) were obtained. The cell growth was inhibited at above 15$\times$$10^{-4} (kcal/cm^2/h)$ of light intensity in an outdoor cultivation. It was also showed that temperature is one of the critical growth parameters in the outdoor cultivation. The hydrocarbon production from D. salina 1650 seems to be partially growth related production process, and these algal hydrocarbons can be used for subsituting petroleum directly or through cracking processes. The value of weight fraction carbon of D. salina 1650 was similar to that of Botryococcus braunii and so was the hydrocarbon productivity.

• KSBB Journal
• /
• 제16권5호
• /
• pp.493-499
• /
• 2001

2차원 형관센서를 이용하여 생물공정을 모니터링하는 기술에 관해 연구하였다. 기존의 광학센서에 비해 2차원 형광센서는 발효공저의 중요한 변수들을 동시에 모니터링하는데 사용될 수 있다. 본 연구에서는 2차원 형광센서를 이용하여 제조합 E. coli, A,. terreus 및 T. vulagis 등의 발효공정을 모니터링하고 미생물의 성장과 생성물의 생성에 대한 형광 스펙트럼의 변화를 정성적으로 비교, 고찰하였다. 2차원 형광센서는 발효공정 이외에도 생물화학반응 및 분리공정에도 쉽게 사용할수 있고 생물공정의 최적화를 도모하는데 이용될 수 있다.

• 산업기술연구
• /
• 제20권A호
• /
• pp.45-50
• /
• 2000

A submerged cultivation of Ganoderma lucidum was carried out in an air-lift fermenter system, and the effects of different pH processes on extracellular branched ${\beta}$-1,3-glucan(EPS) production and mycelial growth(MDW) were investigated. The controlled pH process improved the production of branched ${\beta}$-1,3-glucan and biomass in comparison to the uncontrolled pH process. However, the maximum production of branched ${\beta}$-1,3-glucan were obtained by the bi-staged pH process. From these results, we confirmed that the bi-staged pH process was the most effective for improving the production of branched ${\beta}$-1,3-glucan from submerged culture of G. lucidum.

• KSBB Journal
• /
• 제24권1호
• /
• pp.106-112
• /
• 2009

An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5${\alpha}$, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.

• 한국버섯학회지
• /
• 제16권2호
• /
• pp.125-129
• /
• 2018

ICT 기반의 표고 스마트 재배시설 모델을 개발하기 위하여 외부환경 영향을 감소시킨 재배하우스를 선정하였다. 선정된 재배사 내외부에는 재배환경 변화를 모니터링하기 위한 센서를 설치하였으며 센서에 의해 수집되는 환경변화 데이터를 기반으로 실시간으로 모니터링 및 제어가 가능한 제어반을 제작하였다. 표고재배 환경관리를 효율적으로 진행하기 위하여 재배과정 분석을 통해 4가지 과정으로 구분하고 각 과정에 따라 환경제어 모듈을 설계하였다. 원격 모니터링 및 제어를 위한 PC, 모바일 소프트웨어를 개발하여 재배자가 편리하게 재배환경을 관리하고 보다 안정적으로 버섯을 생산할 수 있는 재배시스템을 개발하였다.

• Journal of Microbiology and Biotechnology
• /
• 제32권4호
• /
• pp.419-429
• /
• 2022

American ginseng (Panax quinquefolium L.) is a perennial herbaceous plant widely cultivated in China, Korea, the United States, and Japan due to its multifunctional properties. In northwest China, transplanting after 2-3 years has become the main mode of artificial cultivation of American ginseng. However, the effects of the cultivation process on the chemical properties of the soil and bacterial community remain poorly understood. Hence, in the present study, high-throughput sequencing and soil chemical analyses were applied to investigate the differences between bacterial communities and nutrition driver factors in the soil during the cultivation of American ginseng. The responses of soil nutrition in different ecological niches were also determined with the results indicating that the cultivation of American ginseng significantly increased the soluble nutrients in the soil. Moreover, the bacterial diversity fluctuated with cultivation years, and 4-year-old ginseng roots had low bacterial diversity and evenness. In the first two years of cultivation, the bacterial community was more sensitive to soil nutrition compared to the last two years. Proteobacteria, Actinobacteria, Gemmatimonadetes, Acidobacteria, Firmicutes, and Bacteroidetes dominated the bacterial community regardless of the cultivation year and ecological niche. With the increase of cultivation years, the assembly of bacterial communities changed from stochastic to deterministic processes. The high abundance of Sphingobium, Novosphingobium, and Rhizorhabdus enriched in 4-years-old ginseng roots was mainly associated with variations in the available potassium (AK), total phosphorus (TP), total potassium (TK), and organic matter (OM).

• 공업화학
• /
• 제24권3호
• /
• pp.285-290
• /
• 2013

최근 비식용 바이오매스 자원인 미세조류를 이용하여 배가스 내의 이산화탄소를 저감함과 동시에 바이오연료를 생산하는 공정이 개발되고 있다. 미세조류 바이오연료 전환 공정은 미세조류 경작, 수확, 추출, 전환 등의 다양한 공정들이 연속적으로 이용된다. 이에 따라 실제 에너지 생산 효과에 대한 우려가 여전히 존재한다. 본 연구는 석탄 발전소에서 배출되는 배가스를 이용하여 파일럿 광생물 반응기에서 생산되는 미세조류를 대상으로 바이오연료 전환에 따른 실에너지 효율을 계산하였다. 에너지 전환 공정은 전이에스터화, 열분해 공정을 선정하였으며 미세조류의 지질 함량에 따른 영향을 검토하였다. 미세조류 바이오연료 전환 공정들은 경작, 수확, 추출, 전환 등에 소요되는 에너지보다 많은 양의 에너지를 가지는 바이오연료를 생산할 수 있으며 지질 함량이 높은 미세조류는 열분해보다 전이 에스터화 반응이 효과적이었다.

• Journal of Ginseng Research
• /
• 제41권2호
• /
• pp.195-200
• /
• 2017

Background: The natural ratios of carbon (C), nitrogen (N), and sulfur (S) stable isotopes can be varied in some specific living organisms owing to various isotopic fractionation processes in nature. Therefore, the analysis of C, N, and S stable isotope ratios in ginseng can provide a feasible method for determining ginseng authenticity depending on the cultivation land and type of fertilizer. Methods: C, N, and S stable isotope composition in 6-yr-old ginseng roots (Jagyeongjong variety) was measured by isotope ratio mass spectrometry. Results: The type of cultivation land and organic fertilizers affected the C, N, and S stable isotope ratio in ginseng (p < 0.05). The ${\delta}^{15}N_{AIR}$ and ${\delta}^{34}S_{VCDT}$ values in ginseng roots more significantly discriminated the cultivation land and type of organic fertilizers in ginseng cultivation than the ${\delta}^{13}C_{VPDB}$ value. The combination of ${\delta}^{13}C_{VPDB}$, ${\delta}^{15}N_{AIR}$, or ${\delta}^{34}S_{VCDT}$ in ginseng, except the combination ${\delta}^{13}C_{VPDB}-^{34}S_{VCDT}$, showed a better discrimination depending on soil type or fertilizer type. Conclusion: This case study provides preliminary results about the variation of C, N, and S isotope composition in ginseng according to the cultivation soil type and organic fertilizer type. Hence, our findings are potentially applicable to evaluate ginseng authenticity depending on cultivation conditions.