• Research in Plant Disease
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• v.19 no.2
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• pp.90-94
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• 2013

Potato cv. 'Haryeong' was bred with high solids, resistance to late blight and good culinary quality. It has been registered as new potato variety in 2005. Tuber necrosis symptoms such as severe superficial necrosis, raised surface lesions and ringed necrotic areas were found in tubers of 'Haryeong' during storage of seed potatoes in 2010. Potato virus Y (PVY) was detected from these symptomatic tubers by the analysis of RT-PCR using a primer set specific to coat protein gene of PVY. The nucleotide sequence of RT-PCR product ($PVY^{Hkr}$) was determined and compared to those of other strains, such as $PVY^{Kor}$, $PVY^N$, $PVY^{NTN}$, $PVY^O$, and $PVY^C$ registered in GeneBank. The result showed that $PVY^{Hkr}$ was exactly the same as $PVY^{Kor}$, Korean isolate reported in 2005, except two nucleotides. To verify the PVY was responsible for the tuber necrosis symptoms shown in the tubers of 'Haryeong', a bioassay was done using two viruses (PVY and Potato leafroll virus) and five potato cultivars ('Haryeong', 'Superior', 'Atlantic', 'Dejima', and 'Chubaek'). As expected, the same necrosis symptom appeared in tubers of 'Haryeong' infected with PVY only during the storage period.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.175-182
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• 1995

Rice is one of the most successful monocot in regenerating fertile and genetically stable transgenic plants. However there is no report of a rice line developed in Korea that can be used for regeneration of fertile and genetically stable transformants. In this paper we first demonstrate that a Korean variety Nakdongbyeo, is suitable to obtain transgenic rice plants. Protoplasts from embryogenic suspension cultures were co-transformed with HPT (hygromycin phosphotransferase) and GUS ($\beta$-glucuronidase) genes in separate plasmids in the presence of PEG (polyethylene glycol). In 5 independent experiment, the average frequency of calli showing hygromycin resistance were 1.73%. Plantlets were regenerated from the Hy $g^{R}$ calli. The average efficiency of plantlet regeneration was apprbximately 27%. Based on the GUS activities of hygromycin resistant calli, ca.35% of the resistant calli carried active GUS genes. The R0 transgenic plantlets were grown to maturity and Rl seeds were obtained. By examining the in siぉ activity of GUS in Rl seeds and seedlings, we confirmed that the GUS transgene driven by a CaMV 35S (cauliflower mosaic virus) promoter showed proper expression patterns. We also confirmed Mendelian segregation of the HPT transgene in the Rl generation.n.

• Korean Journal of Organic Agriculture
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• v.18 no.1
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• pp.75-82
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• 2010

The purpose of this study is to investigate an occurrence rate of rice stripe virus disease under different rice transplanting time and different rice cultivars. After final harvest, the yield of rice infected by rice stripe virus was obtained on Wangchal cultivar compared to uninfected rice. The results obtained as following: 1) Yield of rice infected by rice stripe virus was decreased by 64% with reduction of culm length, pancile length, number of spike, number of spikelet, and grain filling compared to uninfected rice, 2) An occurrence rate of rice stripe virus disease under different rice planting season was high as order of May 30 > June 15 > July 1. In additional, to reduce a rice stripe virus disease on higher disease occurrence region and susceptible cultivars, rice planting season will be accepted on and after June 15, and 3) Eighteen cultivars were resistance cultivars such as Hwayeongbyeo, Onnuri, Ilmibyeo, Nampyongbyeo, Dongjin2ho, Hopumbyeo, Hwangkeumnuri, Malgeumi, Saenuri, Pungmi1ho, Haechanmulgyeo, Hwangkeumnodeul, Chilbobyeo, Dongjinbyeo, Younganbyeo, Junambyeo, Samkwangbyeo, and Nakdongbyeo. Twenty-one cultivars were susceptible cultivars such as Odaebyeo, Unkwangbyeo, Shinungbong1ho, Manabyeo, Hopyongbyeo, Borami, Damibyeo, Hwangchalbyeo, Sulgengbyeo, Nongrim6ho, Sasanishigi, Yunishigari, Chungmubyeo, Dongjin1ho, Huknambyeo, Guromy, Shanghehanghulna, Heukchalbyeo, Heukhwangbyeo, and Aranghwangchalbyeo.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.43-49
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• 2015

This experiment was conducted to evaluate the agronomic characteristics and forage productivity of Italian ryegrass cultivars in Yecheon, Kyeongbuk from 2011 to 2013. The experimental design was a randomized complete block design (RCBD) with three replications. Italian ryegrass cultivars were seeded on Sep. 26 in 2011 and Sep. 25 in 2012. The Italian ryegrass cultivars were harvested on the heading date of the variety. The cold-resistance of domestic cultivars was stronger than other foreign varieties. Among early-maturing cultivars, the dry matter (DM) yield of the "Kowinearly" was 10,031 kg/ha, which was significantly higher than that of other cultivars (p<0.05). In medium-maturing cultivars, the DM yield of the "Kowinmaster" and "Tam 90" were 10,484 and 8,191 kg/ha, respectively. Of the two, the former's DM yield was higher than the latter's (p<0.05). Among late-maturing cultivars, the DM yield of "Hwasan 104" was 10,641 kg/ha, which was similar to that of "Hwasan 101" and significantly higher than the other late-maturing cultivars (p<0.05). According to the results obtained from this study, an early-maturing variety with cold-tolerance and high productivity is required for the paddy field cultivation of an Italian ryegrass cultivar after rice harvest in the mid-northern region of Korea. A medium- or late-maturing variety is more advantageous for the field cultivation of the maximum quantity that is possible. In conclusion, to select which variety/ies to grow, the climate of the growing area, weather conditions, and the cropping system must all be considered.

• Journal of Distribution Science
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• v.16 no.8
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• pp.63-68
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• 2018

Purpose - Lettuce (Lactuca sativ L.) is one of the economically important vegetable crops, which worldwide market value is over 100 billion U.S. dollar. In Korea, about 89.7 kilo ton of lettuce was produced in 3400ha in 2016, recoded as No. 1 vegetable crop in domestic green house production. However, recently, domestic lettuce production and cultivation areas are all getting decreased. Thus, novel approaches are needed to be implemented to revive the production. Research design, data and methodology - In this review paper, we first prioritized the end-user traits which are imperative to positively stimulate the domestic lettuce market and discussed relevant genomics strategies. Especially, we assessed a possibility whether school meal program would be a potential niche market. Results - The genomics technologies, which become widely applied in the crop biotechnology since 2008 when next generation sequencing method was developed, may be a good solution in the crop improvement, efficiently gathering valuable information of agriculturally useful traits. Significantly, in lettuce, the high quality whole genome sequence, based on Lactuca sativa cv. Salinas, is publically available and this genomics platform, thus, would be implemented in lettuce breeding program to innovate relevant end-user traits both for the farmers and customers, including the disease resistance to the Fusarium wilt, productivity under hot weather conditions, various nutritional qualities and so forth. These improvements will boost domestic lettuce industries in the near future. Conclusions - Due to the nutritional distinctions comparing to the western style lettuces, domestic leaf lettuces could be one of the important vegetables in the school meal programs. To make it happen, we would better devise diverse recipes to make a salad with it, instead of only using as a wrap vegetable. Meanwhile, novel lettuce varieties need to be developed, which are favorable to the students and also easy to be handled with while processing. Overall, to achieve international competence in the lettuce industries, we need to create elite lettuce varieties that satisfies domestic farmers as well as customers, suitable to various niche markets, such as school meal program. Thus, efficient breeding programs using genomics approaches should be established in advance and careful monitoring on the preference of the related customers for a niche market be continued persistently.

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.591-595
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• 2012

This study was conducted to investigate the possibility of changes in onion (cv. 'Turbo' and 'Changnyongdaego') bulb quality during storage at room and cold temperature. Increased weight loss was found in the onion bulb stored at both cold and room temperature conditions. 4% weight loss was found in bulbs stored at room temperature and 2% at cold storage as compared to fresh cultivated onion bulb; after three months. Initially, there was no significant difference in the firmness of bulbs between cultivars and storage conditions. Hunter a value of bulb stored at room temperature was higher than the value of bulb stored at cold temperature. 27% and 7.9% decay was found after three months in 'Changnyongdaego' and 'Turbo' cultivars stored under room temperature condition respectively. In contrast, only 2% decay was noticed in both cultivars stored at cold storage condition. Hunter L value of onion bulb gradually decreased in both room and cold temperature storage conditions. In conclusion, quality of onion bulb can be maintained until three months. For long term storage and quality maintenance, under room temperature conditions a resistance cultivar should be selected.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.159-163
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• 2013

The purpose of this study is to observe the growth habit and investigate a possibility of cultivating the GM rice (CaMsrB2-8) as a rice cultivar having drought resistance. Germination viability test showed that there was no significant difference between the drought-resistant GM(CaMsrB2-8) and non-GM (Ilmi) rice which was the parent variety at the GM rice. All the seeds of CaMsrB2-8 and Ilmi germinated after 6 days. Viviparous germination was not found in CaMsrB2-8 and Ilmi that was grown in greenhouse at $23{\pm}2^{\circ}C$ with water spraying for 40 days. Ratooning of CaMsrB2-8 and Ilmi was observed in 7-14 days and found uniform in field condition. CaMsrB2-8 seemed to grow faster than Ilmi. But CaMsrB2-8 and Ilmi were similar in 14-21 days. Both CaMsrB2-8 and Ilmi showed low seed shattering and more than 90% grains were ripened. All the seeds scattered in the paddy soil surface were not germinated after passing the winter. This study suggests that the drought-resistant GM rice was not significantly different with the parent variety of Ilmi in many agronomic characteristics such as wildness traits.

• Korean Journal of Breeding Science
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• v.40 no.1
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• pp.39-47
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• 2008

The 1,874 rice lines were selected from 3,000 Ds insertional mutant pool by Basta herbicide treatment and were surveyed for trait variation and molecular characteristics of genes knocked out by Ds insertion. Compared with "Donjin", an original japonica cultivar used for transformation, Ds insertion mutant pool showed large variation in major agronomic traits including tiller, panicle, and heading etc. Southern blot analysis demonstrated that these lines on the average had two Ds copies in Donjin genome, resulting in 38.4% of one copy, 32.5% of two copies, 16.7% of three copies, and 11.3% of over four copies. GUS analysis showed that 3.9% of lines (73/1,860) had tissue-specific expression in leaves, nodal parts, floral organs such as stigma and pollen, and roots. Data set obtained from agricultural trait variation and molecular characteristics for individual Ds insertional lines would provide researchers with more information for understanding the function of unknown rice genes controlling economically important traits.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.488-495
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• 2009

In recent years, an increasing amount of research has been conducted on the yield loss of soybean crop caused by Riptortus clavatus, a serious pest of legumes. The objective of this study were to examine crop damage caused by various population densities of R. clavatus and determine the association of unique growth and seed characteristics with crop damage rate in conventional soybean fields. Major growth responses to R. clavatus were examined. The cultivars that were not as seriously damaged by R. clavatus were Pungsannamulkong, Sobaegknamulkong, Duyoukong, and Sinpaldal 2, but Myeongjunamulkong, Mallikong, and Hwangkeumkong were significantly (P<0.05) damaged. The rate of seed damage caused by R. clavatus was 8.2% in a group of brown colored pubescence, while the rate was 13.0% in a group of the gray colored. Crop damage by R. clavatus clearly more reduced in cultivars with dark hilum colors including dark brown, brown, and grayish brown than those with yellow hilum colors. Concerning pod colors, crop damage was less significant in cultivars with dark brown pod color than with the light brown. Cultivars with short pod length (4.0-4.5 cm) at full seed (R6) stage showed lower seed damage rates by R. clavatus than those with long pod length (>5.6 cm). Therefore, characteristics of hilum, pod, pubescence colors, and pod length were significantly related and linked to the cultivar resistance to R. clavatus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.17-17
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• 2021

곰취는 주로 쌈용, 장아찌, 나물용 등으로 이용되고 있으며 주로 자생 곰취의 일종인 '곤달비' 라고 하는 종이 많이 재배되고 있다. 일반 곰취에 비해 곤달비는 향이 진하지 않고 씁쓸한 맛이 적어 인기가 많은 종이다. 또한 농가에서는 엽수가 많아 수량이 높기 때문에 재배를 선호하게 된 종이기도 하다. 그러나 곤달비는 수확후 여름을 지나면서 고온건조기에 흰가루병과 식물체가 위축되는 로젯현상으로 잎이 작고 진록색으로 되거나 고사하여 다음해의 수량을 저하시키는 원인이 되고 있다. 이렇게 2~3년 재배를 하면 연작장애와 더불어 결주가 생기기 시작하고 수량이 급격히 감소하는 문제가 발생되는 실정이다. 이러한 문제점을 해결하기 위하여 흰가루병에 저항성이면서 세력이 우수한 한대리곰취와 일반곰취를 교배하여 흰가루병에 강하고 수량성이 뛰어난 계통을 선발하여 그 동안 '쌈마니', '다목이', '곰마니', '수마니', '그린베어' 등 5품종을 육성한 바 있다. 이번에는 용도 다양화를 위하여 잎이 매우 커서 김밥처럼 곰취 밥말 이용을 선발하여 품종을 만들고자 하였다. '대마니' 품종은 2007년 7월에서 9월까지 인공교배를 하여 종자를 얻었으며 2008년 3월에 파종하여 육묘후 포장에 정식하여 생육관리를 하였다. 2013년 생육 및 수량특성 조사에서 일반적으로 많이 재배되는 '곤달비' 종보다 우수한 7033-1번 개체를 선발하였다. 선발된 7033-1번 개체는 2014년부터 2020년까지 노지포장에서 증식되면서 고농G-7호로 계통화 되었으며 2019년부터 2년간의 생육특성 검정을 통하여 최종 선발하였다. 그리고 2020년 농작물직무육성 품종선정위원회에서 신품종으로 선정되어 '대마니(Daemani)'로 명명되었다. '대마니' 품종의 특성은 엽병귀의 색이 연녹색을 띠고 엽병에 털이 있으며 잎 뒷면에 털은 없으나 광택이 없고. 엽맥밀도는 보통이다. 쌈용, 데침쌈용, 묵나물용으로 이용이 가능하다. 특히 엽장과 엽폭이 39cm와 49cm로 '잎이 커서 밥말이용으로 적당하다. 주당 엽수는 54개로 '쌈마니' 품종보다 매우 적다. 주당 수량은 4,961g 으로 '쌈마니' 1,362g 보다 3배 정도 많았다. 추대시는 8월 28일, 개화시는 9월 16일로 '쌈마니' 품종보다 약 6일 정도 늦었다. 흰가루병 저항성 정도는 '쌈마니' 품종의 중도 저항성에 비하여 전혀 병에 감염되지 않는 강한 저항성을 보였다. 이와 같은 특성의 흰가루병 저항성 품종이고 잎이 큰 '대마니' 품종이 농가에 보급되어 출하된다면 밥말이용 등 용도다양화를 통한 새로운 소비창출이 가능할 것으로 기대된다.