• Title/Summary/Keyword: crude protease

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Effect of Dietary Protease(bromelain) Treated Feather Meal on the Performance and Nutrient Utilization in Broilers (단백질분해효소(bromelain) 처리 우모분이 Broiler의 생산성과 영양소 이용율에 미치는 영향)

  • Kim, J.H.;Ko, Y.D.
    • Journal of Animal Science and Technology
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    • v.47 no.2
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    • pp.221-232
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    • 2005
  • This study was carried out to investigate the effect of replacing fish meal or soybean meal with feather meal or bromelain treated feather meal in broiler diets on the performances and nutrient utilization. Two hundred and twenty-five broilers were randomly allotted to five dietary treatrnents((1) control, basal diet; (2) PFM 50, 50 % of the fish meal replaced with bromelain treated feather meal; (3) PFM 100, 100% of the fish meal replaced with bromelain treated feather meal; (4) PSM 20, 20 % of the soybean meal replaced with bromelain treated feather meal; and (5) PSM 40, 40% of the soybean meal replaced with bromelain treated feather meal) in a 5-week feeding trial. In the overall period, body weight gain of the PFM 50(1,807 g), PSM 20(1,816 g) and PSM 40(1,823 g) were the highest and that of the PFM 100 was 1,744 g. The body weight gain of the control(1,698 g) was the lowest(p < 0.05) among treatments. Feed conversion was significantly(p< 0.05) improved when bromelain treated feather meal replaced 20% of the fish meal in the basal diet. Digestibilities of dry matter, ether extract, organic matter and phosphorus were not different among the treatments. Digestibility of crude protein of PFM 50(65.87 %), PSM 20(67.18 %) and PSM 40(67.56%) were the highest, and that of the control(54.49%) was the lowest(p < 0.05) among treatments. Arrunonia and hydrogen sulfide gases from the feces were significantly(p < 0.05) decreased in chicks fed the PFM 50, PSM 20 and GFM 40 diets, when observed after 3 weeks of feeding trials. Feed costs of the control and PFM 50 were 604 and 629 won, respectively but that of PSM 50 was 820 won. Therefore, replacement of fish meal with bromelain treated feather meal in the diets for chicks could be useful for economic production.

Qualify Characteristics of Accelerated Anchovy Sauce Manufactured with B. subtilis JM3 Pretense (B. subtilis JM3 Pretense로 제조한 멸치액젓의 품질특성)

  • Park, J.H.;You, S.G.;Kim, Y.M.;Kim, D.S.;Kim, S.M.
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.5
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    • pp.600-605
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    • 2006
  • B. subtilis JM3 pretense from naturally fermented anchovy sauce was purified in $40{\sim}60%$ ammonium sulfate fraction. In order to accelerate the fermentation of anchovy sauce,2% and 4% of crude B. subtilis JM3 protease were added to 6 month-fermented anchovy sauces, respectively and then the various quality characteristics such as pH, lactic acid, amino-nitrogen, VBN, browning and hydrolysis degree, VBN, and sensory evaluation were analyzed at different storage times. pH was constant during storage time in all samples, whereas lactic acid contents of anchovy sauces hydrolyzed by 2% and 4% proteases were higher than that of control. The amino-nitrogen and volatile basic nitrogen contents of anchovy sauce with 2% and 4% proteases were twice higher than those of control. Anchovy sauces with 2% and 4% pretense increased the hydrolysis rate by 27% and 32%, respectively. Browning degree of anchovy sauce with 4% was higher than those of 2% and control. Anchovy sauce with 2% and 4% proteases was good in sensory evaluation of color, aroma, and taste attributes.

Antioxidant activity of white ginseng extracts prepared by enzyme treatment on V79-4 cells induced by oxidative stress (효소처리에 의한 백삼 저분자 화합물의 V79-4 세포주에 대한 항산화 활성)

  • Kim, Young-Chan;Yim, Joo-Hyuk;Rho, Jeong-Hae;Cho, Chang-Won;Rhee, Young-Kyung
    • Journal of Ginseng Research
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    • v.31 no.4
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    • pp.203-209
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    • 2007
  • This study examined the extraction yields, total phenolic compounds content and the antioxidant activities on V79-4 cells of white ginseng extracts prepared by enzyme treatment. Yields of crude extract were 29.5-76%, and total phenolic compounds content showed 0.45-2.2% according to enzyme treatments. Pectinase treatment group showed the highest values of extraction yields and total phenolic compounds content. Pectinase and a-amylase treatment groups protected V79-4 cell viability(above 50%) against $H_2O_2$-induced oxidative damage. In the result of antioxidant enzyme activity evaluation in cells, enzyme treatments did not show the significant difference of SOD activity (p>0.05). However, pectinase treatment group exhibited increased CAT and GPx activities (p>0.05). Also, pectinase and protease treatment group inhibited MDA formation (>50%) in the lipid peroxidation protection experiment.

Modification of Gut Microbiota and Immune Responses via Dietary Protease in Soybean Meal-Based Protein Diets

  • Song, Minho;Kim, Byeonghyeon;Cho, Jin Ho;Kyoung, Hyunjin;Choe, Jeehwan;Cho, Jee-Yeon;Kim, Younghoon;Kim, Hyeun Bum;Lee, Jeong Jae
    • Journal of Microbiology and Biotechnology
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    • v.32 no.7
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    • pp.885-891
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    • 2022
  • Plant-based protein sources such as soybean meal have low digestibility and are generally promoted accumulation of undigested proteins into the intestine by enzymatic treatments. Moreover, potential intestinal pathogens ferment undigested proteins, producing harmful substances, such as ammonia, amines and phenols, leading to an overactive immune response and diarrhea in weaned pigs. As a solution, dietary proteases hydrolyze soybean-based antinutritive factors, which negatively affect immune responses and gut microbiota. In this study, we investigated the effects of dietary proteases (PRO) in a low-crude protein (CP) commercial diet on the immune responses and gut microbiota of weaned pigs. The experimental design consisted of three dietary treatments: a commercial diet as a positive control (PC; phase1 CP = 23.71%; phase 2 CP: 22.36%), a lower CP diet than PC as negative control (NC; 0.61% less CP than PC), and NC diet supplement with 0.02% PRO. We found that PRO tended to decrease the frequency of diarrhea in the first two weeks after weaning compared with PC and NC. In addition, pigs fed PRO showed decreased TNF-α and TGF-β1 levels compared with those fed PC and NC. The PRO group had a higher relative proportion of the genus Lactobacillus and lower levels of the genus Streptococcus than the PC and NC groups. In conclusion, the addition of PRO to a low CP commercial weaned diet attenuated inflammatory responses and modified gut microbiota in weaned pigs.

Effects of dietary protease supplementation on growth rate, nutrient digestibility, and intestinal morphology of weaned pigs

  • Minho, Song;Byeonghyeon, Kim;Jin Ho, Cho;Hyunjin, Kyoung;Sangwoo, Park;Jee-Yeon, Cho;Kyeong Il, Park;Hyeun Bum, Kim;Jeong Jae, Lee
    • Journal of Animal Science and Technology
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    • v.64 no.3
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    • pp.462-470
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    • 2022
  • The addition of dietary proteases (PRO) to weaner diets hydrolyzes soybean-based anti-nutritive factors and improves weaned pig's dietary digestibility and growth performance. Therefore, this study explores the effects of PRO in a lower crude protein (CP) level diet than that in a commercial diet on the growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. A total of 90 weaned pigs were randomly assigned to 3 dietary treatments with 6 pigs per pen and 5 replicated pens per treatment using a randomized complete block design (block = body weight [BW]): 1) a commercial weaner diet as a positive control (PC; phase1 CP = 23.71%; phase2 CP: 22.36%), 2) lower CP diet than PC as a negative control (NC; 0.61% less CP than PC), and 3) an NC diet with 0.02% PRO. Pigs fed PC and PRO had higher (p < 0.05) final BW, average daily gain, and/or gain to feed ratio for the first three weeks and the overall experimental period than NC. The PC and PRO groups had greater (p < 0.05) apparent ileal digestibility of dry matter, CP, and energy than the NC group. Moreover, pigs fed PC and PRO increased (p < 0.05) apparent total tract digestibility of CP compared with those fed NC. In addition, the PRO group had a higher number of goblet cells than the PC and NC groups. However, pig fed PC and PRO increased (p < 0.05) villus height and height to crypt depth ratio in the ileum compared with those fed NC. In conclusion, PRO supplementation in a commercial weaner diet with low CP levels improves growth rate and nutrient digestibility by modulating the intestinal morphology of weaned pigs.

Three-step in vitro digestion model for evaluating and predicting fecal odor emission from growing pigs with different dietary protein intakes

  • Lo, Shih-Hua;Chen, Ching-Yi;Wang, Han-Tsung
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1592-1605
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    • 2022
  • Objective: The objective of this study was to select an effective in vitro digestion-fermentation model to estimate the effect of decreasing dietary crude protein (CP) on odor emission during pig production and to suggest potential prediction markers through in vitro and in vivo experiments. Methods: In the in vitro experiment, three diet formulations with different CP contents (170 g/kg, 150 g/kg, and 130 g/kg) but containing the same standardized ileal digestible essential amino acids (SID-EAA) were assessed. Each diet was evaluated by two different in vitro gastric-intestinal phase digestion methods (flask and dialysis), combined with fresh pig feces-ferment inoculation. Eighteen growing barrows (31.9±1.6 kg) were divided into three groups: control diet (180 g CP/kg, without SID-EAA adjustment), 170 g CP/kg diet, and 150 g CP/kg diet for 4 weeks. Results: The in vitro digestion results indicated that in vitro digestibility was affected by the gastric-intestinal phase digestion method and dietary CP level. According to the gas kinetic and digestibility results, the dialysis method showed greater distinguishability for dietary CP level adjustment. Nitrogen-related odor compounds (NH3-N, indole, p-cresol, and skatole) were highly correlated with urease and protease activity. The feeding study indicated that both EAA-adjusted diets resulted in a lower odor emission especially in p-cresol and skatole. Both protease and urease activity in feces were also closely related to odor emissions from nitrogen metabolism compounds. Conclusion: Dialysis digestion in the gastric-intestinal phase followed by fresh fecal inoculation fermentation is suitable for in vitro diet evaluation. The enzyme activity in the fermentation and the fecal samples might provide a simple and effective estimation tool for nitrogen-related odor emission prediction in both in vitro and in vivo experiments.

Studies on the Quality of Kochujang Prepared with Grain and Flour of Glutinous Rice (찹쌀과 찹쌀가루가 고추장의 품질에 미치는 영향)

  • Kim, Keun-Hyang;Bae, Jung-Sul;Lee, Taik-Soo
    • Applied Biological Chemistry
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    • v.29 no.2
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    • pp.227-236
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    • 1986
  • Three types of Kochujang(soybean-red pepper paste) were prepared with glutinous rice flour (group A), glutinous rice grain (group B) or the mixture of flour and grain (group C) to investigate some chemical composition, enzyme activities and the texture during aging period. 'There were no remarkable differences in the contents of moisture, crude protein, crude fat, minerals and alcohols, titrable acidity and pH among the tested groups. Amino nitrogen content, acidic protease and saccharogenic amylase activities were measured to be higher values for group A whereas reducing sugar content higher values for group B. All tested groups contained Ca, Cu, K, Mn, Mg, P and Fe, particularly higher amount of K but a trace of Cu, Mn and Fe. All tested groups showed higher content of aspartic acid, glutamic acid, cystine, proline and lower content of histidine and valine 60 days after the preparation. The total free amino acid content was 16.49 mg/g for group A, 13.68 mg/g for group B and 15.84 mg/g for group C. The measurement of texture showed high values of hardness and adhesiveness with group A immediately after the preparation and with group B at the end of aging. Cohesiveness and springiness were high with group A while low with group B throughout aging period.

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Studies on the Sik-hae Fermentation Made by Flat-fish (가자미 식해(食醯)에 관한 연구)

  • 이철호;조태숙;임무현;강주회;양한철
    • Microbiology and Biotechnology Letters
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    • v.11 no.1
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    • pp.53-58
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    • 1983
  • Sik-hae is a traditional Korean fermented fish product which is made from flat-fish, garlic, salt, red pepper and millet. The changes in chemical composition, pH, acidity and the contents of Amino-N, VBN, TMA and organic acids were investigated during the fermentation of Sik-hae. The changes in the microflora, enzyme activity and the sensory quality including textural characteristics were also evaluated. The changes in the contents of crude protein, crude fat and moisture during Sik-hae fermentation were negligible. The pH of the product tended to decrease in the course of fermentation and it showed the minimum value of 4.5 after 7 days of fermentation. On the other hand the acidity continued to increase up to 2300mg % by 4 weeks of fermentation. Lactic acid was the major organic acid. The content of Amino-N in sik-hae gradually increased up to 673.6mg % by 2 weeks of fermentation, and then slightly decreased. The content of VBN increased rapidly during the first 2 weeks of fermentation, while little changes in TMA content was observed. The number of proteolytic bacteria increased slightly for the first 2 weeks and then rapidly decreased. The number of yeast and acid forming bacteria increased rapidly from the 4th day to the 14th day of fermentation and then decreased. Both lipase and protease activities showed the maximum at the 11th day of fermentation. The texture softening of the fish occurred after 1 week of fermentation and the adhesiveness appeared after 2 weeks of fermentation. Summarizing these results, the optimum fermentation time for Sik-hae from flat fish were 2 weeks at 2$0^{\circ}C$ and the quality of the product could be kept for up to 4 weeks in refrigerator.

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Physicochemical and Microbiological Properties of Korean Traditional Meju (한국 재래식 메주의 이화학적 및 미생물학적 특성)

  • Choi, Ki-Soon;Lee, Ho-Joon;Kwon, Dong-Jin
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.217-222
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    • 2009
  • The objective of this study was to obtain basic data on Korean traditional meju collected in 17 regions of Korea, to define and control meju quality. The moisture, crude fat, crude protein, and amino nitrogen contents of meju were 9.83-36.24%(w/w), 17.46-28.74%(w/w), 42.00-45.54%(w/w), and 223.65-1137.68 mg%, respectively. Meju was the enzyme source which made the soy sauce and doenjang. The $\alpha$-amylase, $\beta$-amylase, and protease levels were 130.32-1254.45, 30.07-167.88 and 72.53-340.04 units, respectively. Regional enzyme activities differed widely. Bacterial levels were $4.8{\times}10^7-2.6{\times}10^{10}cfu/g$, and molds and yeasts were at $4.3{\times}10^4-7.9{\times}10^6cfu/g$.

Qualify and Stability of Fish Sauce during Storage (어장유의 품질과 저장안정성)

  • KIM Byeong-Sam;PARK Sang-Min;CHOI Soo-Il;KIM Chang-Yang;HAN Bong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.1
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    • pp.20-26
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    • 1986
  • Very little information is available in the literature on storage of fish sauce. Therefore, microbiological and chemical chracteristics during storage and quality of fish sauce were investigated and discussed to present data about the optimum storage condition. The chopped sardine meat was mixed with equal amount of water and $9\%$(w/w) of $75\%$ vital wheat gluten and then hydrolyzed by addition of commercial proteolytic enzymes such as bromelain, papaya protease, ficin and a enzyme mixture (Pacific Chem. Co.) for 4 hours at $52.5^{\circ}C$. The reaction mixture was heated for 30 min at $100^{\circ}C$ for enzyme inactivation, pasteurization and color development and then centrifuged for 20 min at 4,000 rpm. Table salt and benzoic acid were added for bacteriostatic effect and stored for 80 days at $15{\pm}1^{\circ}C$ and $30{\pm}1^{\circ}C$. The results were summarized as follows: 1. The amount of amino-nitrogen and pH of fish sauce were almost unchanged during storage. 2. Mininum concentration of salt for bacteriostatic activity was $9\%$(w/w) regardless of addition of benzoic acid. 3. the yields of amino-nitrogen were $63.1\%$ for the hydrolysate prepared without enzyme, $79.7\%$ for that with bromelain, $69.9\%$ with ficin, $74.3\%$ with papaya pretense, and $78.1\%$ with enzyme mixture, respectively. 4. The contents of amino-nitrogen were $4510.0mg\%$ on the dry basis for the product prepared by autolysis, $5483.2mg\%$ for that prepared with bromelain, $5305.7mg\%$ with ficin, $4994.1mg\%$ with papaya protease and $5582.3mg\%$ with the enzyme mixture, respectively. 5. The contents of crude protein were $51.35\%$ on the dry basis for the product prepared by autolysis and 55 to $59\%$ for prepared with commercial enzymes. 6. The hydrolysate prepared with the enzyme mixture revealed a little stronger meaty taste than any other products. 7. The level of crude protein in residues was still high ($69.5{\sim}77.2\%$ on the dry basis) and might be originated from the added vital wheat gluten.

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