• Title/Summary/Keyword: crown in and out

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Isolation and identification of Agrobacterium spp. using carrot disc and transformation of Nicotiana gluca by selected strains (당근 절편을 이용한 Agrobacterium spp. 분리, 동정과 선발균주에 의한 연초의 형질전환)

  • Yang, Deok-Chun;Yang, D.C.;Choi, K.T.
    • Journal of the Korean Society of Tobacco Science
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    • v.13 no.1
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    • pp.43-52
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    • 1991
  • These studies were carred out to obtain the transformant from tobacco cells by Agrobacterium spp. from crown gall and soil at the natural field in Korea, and identified their virulence. Kodo's and Clark's selective media were used for isolation of Agrobacterium spp. In these media, total of 99 strains were characterized based on the morphological characteristics of colonies. Among them 34 strains were able to induce on carrot discs. And hypervirulent strains C23-1 and K29-1 were identified as Agrobacterium tumefaciens biotype 1 and biotype 2, respectively. These strains formed fast growing, larger gall as compared to those induced by other strains on the carrot discs. Transformed tobacco callus was initiated on the phytohormone free MS medium with 250$\mu\textrm{g}$/ml carbenicillin after co-cultivation of tobacco stem explants and Agrobacteria. On the phytohormone free media, shoot was rarely formed from transformed callus. However, these shoot were teratoma shoots which were not grown as normal shoot, and teratoma shoot from transformant by C23-1 was smaller than that of K29-1.

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Effects of Nutrient Solution Application Methods and Rhizospheric Ventilation on Vegetative Growth of Young Moth Orchids without a Potting Medium in a Closed-Type Plant Factory

  • Min, Sang Yoon;Oh, Wook
    • Journal of People, Plants, and Environment
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    • v.23 no.5
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    • pp.545-554
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    • 2020
  • Background and objective: Moth orchids in the vegetative stage are suitable for a multi-layer growing environment in a closed-type plant factory which can be a good alternative that can reduce production costs by reducing cultivation time and energy cost per plant. This study was conducted to find out the optimal rhizospheric environment for different irrigation methods without a potting medium and rhizospheric ventilation for the vegetative growth of young Phalaenopsis hybrid 'Blanc Rouge' (P. KV600 × P. Kang 1) and Phalaenopsis Queen Beer 'Mantefon' in a closed-type plant factory system. Methods: The one-month-old clonal micropropagules with bare roots rapped with a sponges were fixed on the holes of styrofoam plates above growth beds, and were watered using the ebb-and-flow (EBB) and aeroponic (AER) methods with Ichihashi solution (0.5 strength) once a day at 06:00 (P) or 18:00 (S), and both (PS). Rhizospheric ventilation (V) was also applied to change the temperature, relative humidity, and CO2 concentration of the beds. Plants potted into sphagnum moss and watered once a week were used as the control group. Results: After 12 months of treatment, the growth characteristics of the EBB groups were the best among the treatment groups without a medium, but no effect of irrigation timing was observed. V reduced the temperature, relative humidity and CO2 concentration of the beds. Whereas, EBB+V (ebb-and-flow with ventilation) improved plant growth and reduced the occurrence of disorders and withering. Especially, EBB+V showed a similar performance to the control group. Conclusion: The results indicated that the optimal irrigation method without a potting medium for producing middle-aged potted moth orchids was the EBB system with forced rhizospheric ventilation. Therefore, further studies on the optimal ventilation method and moisture control of the crown need to be carried out to develop the irrigation system without a potting medium for vertical farming in closed-type plant factories.

A Study on Application of Combustion Products for Forest Fire Investigation (산불화재 감식을 위한 연소생성물의 응용에 관한 연구)

  • Park, Young-Ju;Lee, Hae-Pyeong
    • Journal of the Korean Society of Safety
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    • v.26 no.4
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    • pp.111-119
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    • 2011
  • This study was designed to provide basic data applicable to fire investigation through consideration of combustion products and propose vulnerability of combustibles through analysis of $CO_2$ emission. In order to achieve these research objectives, characteristics of combustion products such as smoke release rate of each part(raw leaves, branches and barks), $CO_2$ emission and ash production were considered targeting on 6 oak species(Quercus variabilis Blume, Quercus aliena Blume, Quercus serrata, Quercus mongolica Fisch, Quercus dentata Sapling and Quercus acutissima) using cone calorimeter and smoke density tester. As a result, it was found that raw leaves release smoke more relatively than branches and barks, when they burn, and that Quercus variabilis Blume has the highest smoke density. Also, Quercus acutissima released CO and $CO_2$ which are respectively, 6.67 times and 1.43 times more than Quercus variabilis Blume with low $CO_2$ emission. In addition, branches released CO and $CO_2$ more relatively. There was a big difference in ash production among raw leaves(3.1 g), branches(10.5 g) and barks(16.43 g). It was identified that Quercus serrata produces ashes which are nearly 9.95 times more than Quercus variabilis Blume. It demonstrates that Quercus serrata contains relatively higher minerals and that Quercus variabilis Blume can leave lots of traces like stain and carbonization, as it releases smoke a lot and it's difficult to predict visibility, when a forest fire breaks out in its community area. It is also considered that smoke particles containing oil in the air leave strain on the surface of a tree, and that CO and $CO_2$ emission increases, when crown fire to burn branches breaks out.

Ziziphus spina christifor Sustainable Agroforestry Farming in Arid Land of Khartoum State of Sudan

  • Mustafa Abdalla Nasre Aldin;Hussein Alawad Seid Ahmed;Mohamed El Mukhtar Ballal;Adil Mahgoub Farah
    • Journal of Forest and Environmental Science
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    • v.39 no.1
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    • pp.20-26
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    • 2023
  • Cow pea (Vigna unguiculata) was intercropped with Ziziphus spina-christi as summer forage in two consecutive seasons of 2017 and 2018. The aims to find out suitable agroforestry practice for saline soils of Khartoum State. And to investigate effect of tree spacing on forage biomass yield under semi -irrigated systems. Completely randomized block design with 3 replicates was conducted for this trial. Thus Z.spina-christi that fixed at 4×4 m was intercropped with cowpea at 1 m and 1.5 m spacing from trees trunk. Tree growth parameters were measured in terms of tree height, tree collar diameter, tree crown diameter and fruit yield per tree. While crop were parameters were determined in terms of plant height, number of plant, forage biomass yield per ha and land equivalent ratio. Soil profile of 1×1 m and 1.5 m depth was excavated and its features were described beside its chemical and physical properties were analyzed for 0-10 cm, 0-30 cm, and 30-60 cm and 60-100 cm layers. The results revealed that soil pH, CaCO3, SAR, ESP, and EC ds/m were increased by increasing soil depths. Meanwhile tree growth in terms of tree height was significant in the first season 2017 when compared with tree collar diameter and tree crown diameter. Also significant differences were recorded for tree growth when compared with sole trees in the second season in 2018. Tree fruit showed marked variations between the two seasons, but it was higher under intercropping particularly at ZS2. Crop plant height was highly significant under sole cropping than intercropping in first season in 2017. In contrast forage biomass yield was significant under intercropping in ZS1 and ZS2 treatments. Land equivalent ratio was advantageous under this agroforestry system particularly under ZS2. Thus it recorded 5 and 9 for ZS2 in the two consecutive seasons respectively. Therefore, it is feasible to introduce this agroforestry system under such arid lands to provide summer forage yield of highly nutritive value and low cost for animals feed as well as to increase farmers' income and to halt desertification and to sequester carbon.

A Study tor 2-Dimensional Analysis Technique for 3-Dimensional Ground Behaviour Due to Tunneling (터널 굴진시의 3차원 지반거동의 2차원적 해석법 고찰)

  • 김교원;이현범
    • The Journal of Engineering Geology
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    • v.6 no.3
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    • pp.111-118
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    • 1996
  • In general, a three dimensional ground behaviour during tunneling is simulated by using two dimensional analysis programs in consideration of a certain ratio of stress or strain distribution to take into account the effect of construction stage by a tunnel face advance. A series of trree dimensional analyses was conducted to deduce a normalized displacement (surface or crown settlement) curve in longitudinal direction, of which curve is reflecting an effect of a tunnel advance under a various condition. And, by using try and error technique, two dimensional analyses were carried out to determine an optimum stress distribution ratio for a settiement curve coincided with the curve obtained by three dimensional analyses. Finally, monitored results from a subway tunnel were compared with two dimensional analysis results for varification of the deduced stress distribution ratio as well as the two dimensional analysis program employed in this study.

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A Study on Revegetation Measures with Recycling Root-stock of Native Tree(I) (자생 수목 그루터기를 이용한 자연식생복원 녹화공법 연구(I))

  • Oh, Koo-Kyoon;Kwon, Tae-Ho;Bae, Jung-Nam;Park, Seok-Gon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.6 no.5
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    • pp.28-39
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    • 2003
  • This study was carried out to elucidate effective restoration measures for natural forest with recycling native tree un site from November 2001 to October 2002 to obtain a basic information for revegetation measure, eight experimental treatment was done and the length of stump, root-ball size of stump, antisepsis treatment of trunk cut, Planting season and contents of organic matter in soil were effective on regrowth of root-stock. Thirteen tree species including Quercus acutissima among twenty tree species showed outstanding sprout and survival rate(over 90 percent), Planting in November and combinated planting with 5 trees and 9 shrubs of root-stock per 100$m^2$ plot showed a good growth. And 10 percent of organic matter plot showed a good crown coverage.

A Study on the Combustion Characteristics of the Crown of Pine Trees in the Drying Season (건조기 소나무 수관부 부위별 연소특성에 관한 연구)

  • Hyuk Kwon;Jong Ho Lee
    • Journal of the Korean Society of Safety
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    • v.38 no.4
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    • pp.39-46
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    • 2023
  • Pine trees, which account for 23% of the forested area of the Republic of Korea, are highly vulnerable to fire in comparison to broad-leaved trees due to the presence of consistent water tube sections throughout the year and resin that is composed of approximately 20% oil. In addition, the pattern of forest fires is determined by weather, topographic conditions, and fluctuation in moisture content. Therefore, when fire breaks out in pine tree forests during the dry season (January to March), it is difficult to extinguish, and it quickly spreads. In this study, the combustion characteristics of pine needles, pine cones, and pine branches in the water tube sections of living pine trees were compared and analyzed in accordance with the moisture content as per the ISO 5660-1. The monthly moisture content was analyzed from January to March, and it was found to be the lowest in March, with 53.6% for pine needles, 51.9% for pine branches, and 10.9% for pine cones. In particular, pine cones were more vulnerable to fire as compared to pine needles and pine branches because their moisture content was more than five times lower than that of pine needles and branches. The ignition time, which affects the speed of flame propagation, was the most rapid in March, and the fastest ignition time was for pine cones, at 19 seconds, followed by 34 seconds for pine needles, and 256 seconds for pine branches. The pine branches were the last to be ignited due to the effect of density, according to the thickness and specific gravity of the specimen. The peak heat release rate, which is a measurable index of fire intensity, was analyzed for pine cones and found to be 184.28 kW/m2 , while the mean effective heat of combustion was 19.79 MJ/kg, and the total heat release rate was 39.7 MJ/m2 , and these values were higher than those of pine branches and pine needles. Thus, we determined that the flame propagation speed and fire intensity according to the moisture content can be used to evaluate the risk of fire to the water tube section of pine trees. It is suggested that because of the combustion characteristics of the pine cone in March, that is when the forest is most vulnerable to fires.

Nutrient Environment of Japanese Cedar(Cryptomeria japonica) -I. Changes of Soil Chemical Properties in Grasslands Afforestation (제주도(濟州道) 삼나무(Cryptomeria japonica) 조림지(造林地)의 영양환경(營養環境)에 관(關)한 연구(硏究) -I. 원야조림(原野造林)에 의한 토양성질(土壤性質)의 변화(變化))

  • Jin, Hyun-O
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.3
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    • pp.177-182
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    • 1991
  • This study was carried out to clarify the effects of grassland afforestation on some chemical properties of the Japanese ceder(Cryptomeria japonica) forest soil in Cheju Island. The following results were obtained in this study ; 1. T-C, T-N, exch. cations and base saturation in the surface soil(0~10cm) decreased sharply from planting to crown closure. However, after crown closure, exch. Ca and Mg increased considerably. On the other hand, there were no definit changes below surface soil. 2. In vertical distribution of chemical properties, T-C, T-N, exchangeable acidity and exch. cations decreased with soil depth. Especially, exch. Ca and Mg of surface soil in closed stand show a higher value than that in open stand, but there were no variation below surface soil. 3. In closed stand, the amounts of exch. Ca, Mg and K were 1.8 times, 1.6 times and 1.5 times greater than those in open stand. There was no significant difference between open and closed stand in T-C and T-N. 4. The ratios of the amounts of nutrients in surface soil to those in mineral horizons(ha, 50cm) between the open and closed stand showed 30% and 50% for exch. Ca, 30% and 40% for exch. Mg, 38% and 38% for exch. K, respectively.

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Assessment of influence of old mine gangway on stability of road tunnel in mine area (광산지역 도로 터널링에 있어 폐갱도가 터널 안정성에 미치는 영향 평가)

  • Synn, Joong-Ho;Shin, Hee-Soon;Sunwoo, Choon;Park, Chan
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.4 no.2
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    • pp.123-133
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    • 2002
  • In the construction of the road tunnel in mine area, old mine gangways can cause the instability of the tunnel. In this case study, the field investigation is carried out to figure out the location of old gangways adjacent to the tunnel, and their influence on the tunnel stability is estimated according to the location pattern and rock condition by FLAC analysis. The grouting reinforcement of tunnel crown region and old gangway is suggested and its role on assurance of the tunnel stability is also verified. It can be said from this study that the effect of the old gangway on the stability of tunnel varies with the dimension of gangway, distance from the tunnel, rock condition and groundwater, and therefore these paramerter should be compositively considered in the assessment of the tunnel stability.

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Monitoring of Heavy Metals in Vegetables in Korea (국내 유통 중인 채소류의 중금속 모니터링)

  • Go, Myoung Jin;Lee, Jin Ha;Park, Eun Heui;Park, Sang Wook;Kim, In Kyung;Ji, Young Ae
    • Journal of Food Hygiene and Safety
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    • v.27 no.4
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    • pp.456-460
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    • 2012
  • This study was carried out as a survey on the level of lead (Pb), cadmium (Cd), arsenic (As) and mercury (Hg) in 5 items of agricultural products (lettuce, pumpkin, head lettuce, cabbage and crown daisy) for which safety guidelines are not yet established in Korea. The 407 samples were collected from local markets in 9 regional places and the levels of metals were measured by ICP-MS and mercury analyzer. The average levels of Pb, Cd, As and Hg were 0.026, 0.018, 0.008 and 0.003 mg/kg for lettuce, 0.002, 0.001, 0.001 and 0.0006 mg/kg for pumpkin, 0.002, 0.005, 0.001 and 0.0005 mg/kg for head lettuce, 0.001, 0.002, 0.0002 and 0.0006 mg/kg for cabbage and 0.022, 0.014, 0.006 and 0.004 mg/kg for crown daisy. For risk assessment, the daily dietary exposures of Pb, Cd and Hg by intake of these agricultural products were calculated and compared with PTWI (provisional tolerable weekly intake) established by JECFA. The daily dietary exposure of heavy metals by intake of these agricultural products were 0.13, 0.56 and 0.09% of PTWI, respectively. These monitoring results will be utilized as fundamental data for the establishment of Korean standards of Pb, Cd, As and Hg in 5 items of agricultural products and valuable source for DB construction for science-based safety control of heavy metals in foods including agricultural products.