• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1513-1518
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• 2005

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.

• 전기의세계
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• v.30 no.9
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• pp.582-588
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• 1981

The laser parameters such as a population inversion density, a stimulated emission cross section and loss factor in the glass laser amplifier medium were experimentally determined in order to analyse the properties of laser amplification. Using these parameters, the rate equations were approximately solved and the properties of laser amplification were analysed. An experiment of two-stage amplifier of Q-switching laser pulse was performed and the maximum output power was 1.1 GW, 33J. The several problems such as a laser solarization and a laser coupling in laser amplification were resolved.

• Journal of Microbiology and Biotechnology
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• v.31 no.4
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• pp.610-620
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• 2021

There has been increasing interest in the head and neck squamous cell carcinoma (HNSCC) that is caused by high-risk human papillomavirus (HR-HPV) and has posed a significant challenge to Otolaryngologists. A rapid, sensitive, and reliable method is required for the detection of HR-HPV in clinical specimens to prevent and treat HPV-induced diseases. In this study, a multiple cross-linking spiral amplification (MCLSA) assay was developed for the visual detection of HPV-16. In the MCLSA assay, samples were incubated under optimized conditions at 62℃ for 45 min, and after mixing with the SYBR Green I (SGI) dye, the positive amplicons showed bright green fluorescence while the negative amplicons exhibited no obvious change. The specificity test revealed that the developed MCLSA technique had high specificity and could effectively distinguish all five HPV-16 strains from other pathogenic microorganisms. In terms of analytical sensitivity, the limit of detection (LoD) of MCLSA assay was approximately 5.4 × 101 copies/tube, which was 10-fold more sensitive than loop-mediated isothermal amplification (LAMP) and RT-PCR. The detection results of laryngeal cancer specimens collected from 46 patients with suspected HPV infection in the Liaoning region demonstrated that the positive detection rates of MCLSA and hybridized capture 2 kit were 32.61% (15/46). The true positive rate of the MCLSA assay was higher than that of RT-PCR (100% vs. 93.33%) and LAMP (100% vs. 86.67%). Therefore, the MCLSA assay developed in the present study could be a potentially useful tool for the point-of-care (PoC) diagnosis of HR-HPV, especially in resource-limited countries.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.36 no.10
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• pp.741-747
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• 1987

The high-power Nd:glass system with five-stage amplifier was designed and its amplification characteristics was studied for developing high-power Nd:glass laser system as an energy driver of inertial confinement fusion(ICF). In order to study the amplification characteristics of remporal and spacial Gaussian laser beam, the dependence of them on pumping efficiency and rod loss were studied and discussed. The output energy of this system using phosphate Nd glass rod(LHG-7,LHG-8) and silicate Nd glass rod(LSG-91H), respectively, was calculated by the computer simulation using Avizonis-Grotbeck and Frantz Nodvik equations. As results of this simulation, it was found that the shorter the risetime of laser pulse, the larger the amplification factor and that the larger peak value of laser pulse, the lower the amplification factor. The output inergies of 179J, 344J, and 7J were obtained by the designed five-stage amplified high-power Nd:glass laser system using glass rods of LHG-7,LHG-8, and LSG-91H, respectively. From the results it was found that the laser system using the LHG-8 glass rod was the most excellent one among the systems and the cross section for stimulated emission of the gain coefficient was essentially important parameter for the amplification characteristics.

• Korean Journal of Plant Taxonomy
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• v.50 no.3
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• pp.327-332
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• 2020

Microsatellite markers were developed as a tool for phylogeographic studies of Hosta capitata. We also assessed cross-amplification in species closely related to Hosta capitata. We produced 28 polymorphic microsatellite markers by mapping 300 bp paired-end reads obtained from Illumina MiSeq data of H. capitata. In H. capitata, the number of alleles per locus ranged from 1 to 13. Observed and expected heterozygosity ranged from 0.000 to 0.844 and 0.000 to 0.832, respectively. Additionally, 13 loci were successfully transferable to the related species of H. minor and H. venusta. These markers will provide a powerful genetic tool not only for elucidating the phylogeographic patterns of H. capitata populations but also for studying the genetic delimitation of H. capitata from its related species.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.70-72
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• 2003

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.11
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• pp.1610-1624
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• 1998

An axisymmetric jet is forced with two helical fundamental waves of identical frequency spinning in opposite directions and an additional axisymmetric sub harmonic wave. The subharmonic component rapidly grows downstream from subharmonic resonance with the fundamental, significantly depending on the initial phase difference. The variations of the subharmonic amplitude with the initial phase difference show cusp-like shapes. The amplification of the sub harmonic results in 'vortex pairing of helical modes'. Furthermore, azimuthal variation of the amplification induces an asymmetric jet cross-section. When the initial subharmonics is imposed with an initial phase difference close to a critical value, the jet-cross section evolves into a three-lobed shape. One lobe is generated by the enhanced vortex pairing and the other two lobes are generated by the delayed vortex pairing. Thus, it is confirmed that the initial phase difference between the fundamental and the subharmonic plays an important role in controlling the jet cross-section.

• Research in Plant Disease
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• v.27 no.1
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• pp.38-44
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• 2021

Xylella fastidiosa is the most damaging pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assay were developed to mqsA gene of citrate-synthase (XF 1535) X. fastidiosa and evaluated for specificity and sensitivity. Both assays were more robust than current published tests for detection of X. fastidiosa when screened against 16 isolates representing the four major subgroups of the bacterium from a range of host species. No cross reaction with DNA from healthy hosts or other species of bacteria has been observed. The LAMP and PCR assays could detect 10-4 pmol and 100 copies of the gene, respectively. Hydroxynaphthol blue was evaluated as an endpoint detection method for LAMP. There was a significant color shift that signaled the existence of the bacterium when at least 100 copies of the target template were present.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.481-486
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• 2001

The hydrodynamic instability of the three-dimensional boundary layer on a rotating disk introduces a periodic modulation of the mean flow in the form of stationary cross flow vortices. Detailed numerical values of the growth rates, neutral curves and other characteristics have been calculated for the Type II-instabilities. Presented are the neutral stability results concerning the two instability modes by solving new linear stability equations reformulated not only by considering whole convective terms but by correcting some errors in the previous stability equations. The present stability results are agree with the previously known ones within reasonable limit. The spatial amplification contours have been calculated for the moving disturbance wave, whose azimuth angle is between $\varepsilon=-10^{\circ}$ and $-20^{\circ}$. The transition flow of the moving disturbance wave will be developed at $\varepsilon=-15^{\circ}$ and Re=352 corresponding at the growth rates n = 5.8 from the spatial amplification contours.

• The Plant Pathology Journal
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• v.38 no.2
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• pp.159-166
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• 2022

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42℃ for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.