• 대한바이러스학회지
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• 제28권1호
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• pp.31-38
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• 1998

In Korea, all domestic made test systems for detecting antibodies in HIV-1 contain the antigens from human immunodeficiency type 1 (HIV-1) subtype B. However, because HIV-1 subtype O is significantly different in amino acid sequences from all other subtypes of HIV-1, there has been a need for developing a test for detecting antibodies in subtype O. For this purpose, the entire nucleotide sequence corresponding to the extracellular domain of the transmembrane glycoprotein of HIV-1 subtype O was synthesized with consideration of Escherichia coli condon usage. Various regions of the extracellular domain were cloned into E. coli expression vectors and tested for levels of protein production. The nucleotide sequence, named ECTM, that can encode a 129 amino acid-long peptide, was found to be expressed at a high level in E. coli. The protein of approximately 17 kDa specifically reacted with sera from individuals infected with HIV-1 subtype O. The ECTM protein was purified to near homogeneity by the CM-T gel chromatography, using concentrated, denatured inclusion bodies. In Western blot analysis, the purified viral antigen reacted with sera from individuals infected with subtype O more efficiently than subtype B. The enzyme linked immunoabsorbent assay (ELISA) system was developed using the subtype O viral protein and compared with the commercially available kit lacking the antigens from subtype O. The ELISA kit containing the subtype O antigen ECTM alone efficiently reacted with sera from individuals infected with subtype O. The subtype O antigen-containing kit produced a positive absorbence even when sera were diluted 512-fold, suggesting a high sensitivity. The commercially available kit also reacted with subtype O sera, but produced a negative result at a dilution of 8-fold. Our results suggest that the currently available kit may not be able to efficiently detect subtype O sera and that the viral protein developed in this study may be added to the current system to maximize the detection of sera from individuals infected with subtype O.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2385-2390
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• 2015

Background: Invasive ductal (IDC) and lobular (ILC) carcinomas are the common histological types of breast carcinoma which are difficult to distinguish when poorly differentiated. Discoidin domain receptor (DDR1) and Drosophila dishevelled protein (DVL1) were recently suggested to differentiate IDC from ILC. Objectives: To assess the expression of DDR1 and DVL1 and their association with histological type, grading and hormonal status of IDC and ILC. Materials and Methods: This cross sectional study was conducted on IDC and ILC breast tumours. Tumours were immunohistochemically stained for (DDR1) and (DVL1) as well as estrogen receptor (ER), progesterone receptor (PR) and C-erbB2 receptor. Demographic data including age and ethnicity were obtained from patient records. Results: A total of 51 cases (30 IDCs and 21 ILCs) were assessed. DDR1 and DVL1 expression was not significantly associated with histological type (p=0.57 and p=0.66 respectively). There was no association between DDR1 and DVL1 expression and tumour grade (p=0.32 and p=1.00 respectively), ER (p=0.62 and 0.50 respectively), PR (p=0.38 and p=0.63 respectively) and C-erbB2 expression (p=0.19 and p=0.33 respectively) in IDC. There was no association between DDR1 and DVL1 expression and tumour grade (p=0.52 and p=0.33 respectively), ER (p=0.06 and p=0.76 respectively), PR (p=0.61 and p=0.43 respectively) and C-erbB2 expression (p=0.58 and p=0.76 respectively) in ILC. Conclusions: This study revealed that DDR1 and DVL1 are present in both IDC and ILC regardless of the tumour differentiation. More studies are needed to assess the potential of these two proteins in distinguishing IDC from ILC in breast tumours.

• 복식
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• 제58권10호
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• pp.65-77
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• 2008

The purpose of this study is to analyze the correlation between the density, the Count and the width of cross section in 2D function through comparison the difference of simulated fabrics based on the various yarns, and to compare the 3D effect by Lay Mapping of diverse fabrics. The method of research is to weave the eight fabrics composed of cotton, linen, worsted, slender yarn, loop, $m{\acute{e}}lange$, woolen, and yarn twist with Hi-Tex program, and to practice 3D mapping with Hi-Print program. As a mapping object, the flared skirt which is a basic costume item is selected. As a result, the thickness of yarn in CAD system was fixed by the width of cross section rather than Count, especially by the width of core section not including the fluff section. The type of yarn such as cotton yarn, linen yarn, and worsted had effect on the shape of texture, but had few interrelations with dimension. In the case of 3D mapping, the textural characteristic and the dimension were presented precisely, whereas there were several limitations. First, the thickness of tissue has not been represented. Secondly, the effect of texture such as fuzzy look, loop was not expressed on the skirt outline including sideline and hemline. Thirdly, the difference of silhouette was not distinct. The common point in 2D and 3D operations is that the representation of texture is relatively accurate and that is difficult to measure and manifest of thickness, the side. For more professional digitalizing in fashion industry, above all in the domain of 3D, it must be supplement the subdivided and differentiated mapping process according to the texture, deviating from the existing analog-based organization which has to designate the form and silhouette suitable for tissue.

• 한국전기전자재료학회논문지
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• 제32권6호
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• pp.472-476
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• 2019

We have proposed a novel planar lightwave circuit (PLC) optical sensor to monitor the contamination in a flow-cell where water is continuously supplied through a water quality measurement system. We designed a PLC chip with a V-shape waveguide and the simulated its function as a sensor for monitoring contamination in a flow-cell using a numerical the FDTD (finite-difference time-domain) analysis. A novel cross type of waveguide was introduced to make the PLC chip of the V-shaped waveguide. The fabricated PLC was cut into the cross waveguide. A change in the optical propagation loss of the PLC sensor was observed after immersing the PLC sensor into city water. It was determined that the propagation loss of the PLC sensor was 3 dB at a wavelength of $1.55{\mu}m$ in the city water for 15 days.

• 대한수의학회지
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• 제37권3호
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• pp.547-554
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• 1997

Galectin-3 is known as an animal ${\beta}$-galactoside-binding lectin charicterized with S-type carbohydrate recognition domain. It plays a role in growth, adherence and movement of cells. It is, also, related to the cell transformation and metastasis of tumor cells. In this study, we have expressed and purified recombinant human galectin-3 (rHgalectin-3) using E coli system and asialofetuin affinity chromatography for the future development of monoclonal antibody to Hgalectin-3, which is suggested as the tumor marker for the gastric and thyroid gland cancers. Expressed protein was confirmed as the Hgalectin-3 by immunoblot with cross-reactive murine monoclonal antibody. Lectin activity and specificity of purified protein were, also, confirmed by the competitive inhibition with galectin-3 specific carbohydrate, lactose. Like physiological galectin-3, lectin activity of the molecule was not changed in nonreduced condition. Dimer formation, furthermore, was observed at high concentration of the protein even in the reduced condition, which is well known in physiological galectin-3. These results showed purified rHgalectin-3 has the same activity and molecular nature compared to the physiological galectin-3.

• 한국CDE학회논문집
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• 제7권3호
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• pp.148-156
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• 2002

Most Rapid Prototyping (RP) processes adopt a solid Computer Aided Design (CAD) model, which will be sliced into thin layers of constant thickness in the building direction. Each cross-sectional layer is successively deposited and, simultaneously, bonded onto the previous layer; and eventually the stacked layers from a physical part of the model. A new RP process, the transfer-type Variable Lamination Manufacturing process using expandable polystyrene foam sheet (VLM-ST), has been developed to reduce building time and to improve the surface finish of parts with the thick layers and a sloping surface. This paper describes the generation of Unit Shape Layer (USL), the cutting path data of the linen. hotwire cutter for the VLM-ST process. USL is a three-dimensional layer with a thickness of more than 1 mm and a side slope, and it is the basic unit of cutting and building in the VLM-ST process. USL includes data such as layer thickness, positional coordinates, side angles of each layer, hotwire cutting speed, the heat input to the hotwire, and reference shape. The procedure of generating USL is as follows: (1)Generation of the mid-slice from the CAD model, (2)Conversion of the mid-slice into a simply connected domain, (3)Generation to the reference shape for the mid-slice, (4)Calculation of the rotation angle of the hotwire of the cutting system.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2749-2752
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• 2012

Background: Breaking bad news to cancer patients is a delicate and challenging task for most doctors. Better understanding of patients' preferences in breaking bad news can guide doctors in performing this task. Objectives: This study aimed to describe the preferences of Malaysian cancer patients regarding the communication of bad news. Methodology: This was a cross-sectional study conducted in the Oncology clinic of a tertiary teaching hospital. Two hundred adult cancer patients were recruited via purposive quota sampling. They were required to complete the Malay language version of the Measure of Patients' Preferences (MPP-BM) with minimal researcher assistance. Their responses were analysed using descriptive statistics. Association between demographic characteristics and domain scores were tested using non-parametric statistical tests. Results: Nine items were rated by the patients as essential: "Doctor is honest about the severity of my condition", "Doctor describing my treatment options in detail", "Doctor telling me best treatment options", Doctor letting me know all of the different treatment options", "Doctor being up to date on research on my type of cancer", "Doctor telling me news directly", "Being given detailed info about results of medical tests", "Being told in person", and "Having doctor offer hope about my condition". All these items had median scores of 5/5 (IQR:4-5). The median scores for the three domains were: "Content and Facilitation" 74/85, "Emotional Support" 23/30 and "Structural and Informational Support" 31/40. Ethnicity was found to be significantly associated with scores for "Content and Facilitation" and "Emotional Support". Educational status was significantly associated with scores for "Structural and Informational Support". Conclusion: Malaysian cancer patients appreciate the ability of the doctor to provide adequate information using good communication skills during the process of breaking bad news. Provision of emotional support, structural support and informational support were also highly appreciated.

• 한국전자통신학회논문지
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• 제18권5호
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• pp.999-1008
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• 2023

당뇨병과 같은 만성 질환의 조기 예측은 중요한 이슈이며, 그중에서도 당뇨 예측의 정확도 향상은 매우 중요하다. 당뇨 예측을 위한 다양한 기계 학습 및 딥 러닝 기반 방법론을 도입하고 있으나, 이러한 기술들은 다른 방법론보다 더 우수한 성능을 위해 대량의 데이터를 필요로 하며, 복잡한 데이터 모델 때문에 학습 비용이 높다. 본 연구에서는 pima 데이터셋과 k-fold 교차 검증을 사용한 DNN이 당뇨 진단 모델의 효율성을 감소시킨다는 주장을 검증하고자 한다. 의사 결정 트리, SVM, 랜덤 포레스트, 로지스틱 회귀, KNN 및 다양한 앙상블 기법과 같은 기계 학습 분류 방법을 사용하여 어떤 알고리즘이 최상의 예측 결과를 내는지 결정하였다. 모든 분류 모델에 대한 훈련 및 테스트 후 제안된 시스템은 ADASYN 방법과 함께 XGBoost 분류기에서 최상의 결과를 제공하였으며, 정확도는 81%, F1 계수는 0.81, AUC는 0.84였다. 또한 도메인 적응 방법이 제안된 시스템의 다양성을 보여주기 위해 구현되었다. LIME 및 SHAP 프레임워크를 사용한 설명 가능한 AI 접근 방식이 모델이 최종 결과를 어떻게 예측하는지 이해하기 위해 구현되었다.

• 한국의료질향상학회지
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• 제11권1호
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• pp.32-45
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• 2004

Objective : The aim of this study were to measure quality of life(QOL) in liver transplant recipients, to compare QOL between living donor liver transplant recipients and cadaveric liver transplant recipients and to investigate whether SF-36 may be used as a disease-specific instrument in liver transplant recipients. Methods : We conducted a single-center cross-sectional study of 133 LT recipients ages 13 to 65 years, all of whom had had Liver Transplantation(LT) at least 1 months previously. QOL was assessed using a self-completion questionnaire consisting of the Bang Whal Ran(1991) instruments and the 36-Item Short-Form Health Survey(SF-36) health status profile measure. We investigated whether the SF-36 instrument may be used as a disease-specific instrument in LT recipients. Individual scale scores range from 0 to 100, with higher score reflecting better health. Data on demographics, clinical status at pre transplantation 1 day, post transplantation clinical status, and graft function were collected to identify predictors of post transplantation QOL. Results : Standard measures for test-retest reliability, internal consistency, and discriminant and concurrent validity were examined. The reliability of the SF-36, as measured by test-retest correlation(Pearson coefficients: 0.729, p=0.002) and by internal consistency(Cronbach's alpha: 0.9431) exceeded conventional acceptability criteria. The correlation between domain scores of SF-36 and the Bang Whal Ran(l991) was clear and logical in that the clinical characteristics of SF-36 strongly correlated with the clinical component summary score of the Bang Whal Ran(l991)(r = 0.8155, P<.01). SF-36 scale scores were compared between Cadaveric Liver Transplant recipients and Living Donor Liver Transplant recipients. Donor types of post LT did not influence HRQOL(p>0.05). 87% of the liver transplant recipients were satisfied to get LT. Satisfaction of post LT showed significantly greater HRQOL(p<0.001). Conclusion : SF-36 is found reliable and valid. This study indicates thet Donor Type did not influence HRQOL after LT. The information gained from this study will help us to better define expectations and the clinical course after liver transplantation to patients and their families.

• 한국자기학회지
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• 제23권6호
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• pp.200-204
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• 2013

마그네트론 스퍼터를 이용하여 반강자성/비자성/강자성의 삼층박막 $50{\mu}m{\times}50{\mu}m$ 크기의 십자 형태의 PHR 자기센서를 제작하고, 도메인스코프를 이용하여 외부자기장에 의한 PHR 센서의 자화거동을 관찰하였다. 또 자기저항 신호로부터 센서 특성 및 자기 민감도를 측정하였다. 삼층 구조의 PHR 센서에서 자기이력곡선 이동으로부터 측정한 교환결합력은 20 Oe였으며, $20^{\circ}$에서 자기 민감도는 $20{\mu}V/Oe$이였다. 이를 이용하여 자기 모멘트가 $10^{-13}emu$인 자기 박테리아를 $1{\times}10^3$개 정도의 분해능까지 측정하였으며, 또한 자기 민감도와 박테리아에 의한 출력 전압을 이용해 계산한 결과 박테리아 한 마리가 가지는 자기장의 크기는 $5{\times}10^{-5}Oe$이였다.