• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.85.1-85
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• 2003

In most ascomycetes, a single mating type locus, MAT, with two alternate forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes these alternate idiomorphs reside in different nuclei. In contrast, most homothallic ascomycetes carry both MAT1-1 and MAT1-2 in a single nucleus, usually closely linked. An example of the latter is Gibberella zeae, a producer of mycotoxins such as trichothecene and zearalenone that threaten human and animal health. We asked if G. zeae could be made strictly heterothallic by manipulation of MAT. Targeted gene replacement was used to differentially delete MAT1-1 or MAT1-2 from a wild type haploid MAT1-1 MAT1-2 strain, resulting in MAT1-1；mat1-2, mat1-1；MAT1-2 strains that were self-sterile, yet able to cross to wild type testers and more importantly, to each other. These results indicated that differential deletion of MAT idiomorphs eliminates selfing ability of G. zeae, but the ability to outcross is retained. To our knowledge, this is the first report of complete conversion of fungal reproductive strategy from homothallic to heterothallic by targeted manipulation of MAT. Practically, this approach opens the door to simple and efficient procedures for obtaining sexual recombinants of G. zeae that will be useful for genetic analyses of mycotoxin production and other traits, such as ability to cause disease.

• Journal of Korean Academy of Nursing
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• v.39 no.5
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• pp.613-621
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• 2009

The purposes of this study were to evaluate symptom experience and quality of life (QOL) and to identify the predictors of QOL among breast cancer survivors. Methods: A cross-sectional study was conducted on 200 disease-free breast cancer survivors at two hospitals between December 2007 and July 2008. Functional Assessment of Cancer Therapy Scale-B, Memorial Symptom Assessment Scale-short Form and The Linear Analogue Self Assessment Scale were used to assess symptom experience and QOL in these patients. Data were analyzed using the Pearson correlation, t-test, ANOVA, and stepwise multiple regression with SPSS/WIN 12.0. Results: The mean score of QOL for breast cancer survivors was 95.81 (${\pm}18.02$). The highest scores among physical and psychological symptoms were sexual interest and anxiety. Year since treatment completion was significantly associated with QOL in sociodemographic variables. Physical and psychological symptoms have a significant negative association with QOL. The results of the regression analyses showed that physical and psychological symptoms were statistically significant in predicting patients' QOL. Conclusion: Symptom experience and QOL are essential variables that should be acknowledged when delivering health care to breast cancer survivors. More attention to the reduction and management of psychological distress could improve QOL among breast cancer survivors.

• Research in Community and Public Health Nursing
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• v.29 no.3
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• pp.267-278
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• 2018

Purpose: This study is to investigate working conditions including job stress among visiting nurses in public health centers in Korea. Methods: An social network based mobile survey was conducted in May 2017 (N=936, response rate: 47.0%). Results: The visiting nurses in this study had their average total career as a nurse is 13.7 years. The 68.3% of them were employed in an indefinite term, 17.0% were hired in a fixed term, and 11.0% came from outsourcing. They responded as high job-stress level including inadequate compensation (71.22/100) and job demands (71.91/100). They experienced down-talk (63.4%), swearwords (32.9%), being made a dirty face (39.9%), sexual jokes (30.8%), or being likened or evaluated with their appearance sexually (14.3%). Among the causes of job related conflicts and discrimination, deprived salary level was the most frequent reason (83.4%). The conflicts and discrimination were incurred by government officers (52.4%). There were no significant differences in overall job stress, emotional labor, organizational commitment, violence, and discrimination experience based on their employment types. Conclusion: The differences in working conditions among the non-regular nurses were trivial, and their overall working conditions were poor. It is necessary to improve non-regular nurses' working conditions in order to make up the limitations of the Korean healthcare system which is centered on hospitals.

• Industry Promotion Research
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• v.5 no.1
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• pp.107-115
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• 2020

This study is to find social welfare practices that married women migrant can provide to them in order to relieve the stress of cultural differences and enjoy a good family life. Results,First, action plans to overcome intercultural differences are needed. To overcome effective cross-cultural differences, interventions and goals are set for culturally diverse clients that match their racial, cultural, ethnic, gender and sexual orientation.Second, it is necessary to cultivate professional education personnel. In order to train professionals, cultural diversity should be addressed in existing social welfare courses.Third, the related laws need to be rearranged. The Multicultural Family Support Act continues to emerge as a need for supplementation, including the understanding of Korean family culture and the lack of counseling for immigrant women.As a result, married women immigrants recognize that Korean life is different from their own in many aspects of their daily lives, so they endeavor to have a lot of patience and high level of effort in the adaptation process. Therefore, there is a need for a social welfare practice that can support this.

• Physical Therapy Rehabilitation Science
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• v.8 no.4
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• pp.234-241
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• 2019

Objective: To investigate the physical therapists' actual experience of workplace violence and examine its relationship with depression, burnout, subjective health status, and job and life satisfaction. Design: Cross-sectional study. Methods: The level of workplace violence was assessed using the Korean Workplace Violence Scale composed of 24 questions in the following 4 subscales of workplace violence. Depression was assessed using the Korean version of the Patient Health Questionnaire-9. Burnout was assessed using the Korean version of the Maslach Burnout Inventory-Human Services Survey, which is a 5-item scale. Subjective health status was measured on a 5-point scale. Job and life satisfaction was measured based on the assessment tool used in the World Values Survey. Results: There was a significant correlation between the three subscales of workplace violence and health indicators, including depression, subjective health status, job and life satisfaction (p<0.05). The organizational protective system subscale showed a significant negative correlation with depression and a positive correlation with subjective health status as well as job and life satisfaction (p<0.05). The area of 'psychological and sexual violence from customers' showed a significant moderate correlation with depression and job satisfaction (p<0.05). Also, there was a negative correlation that was significant between depression and subjective health status, job satisfaction, and life satisfaction (p<0.05). Conclusions: This study suggested that future studies should continue to investigate and reveal the causal relationships between workplace violence and physical therapists' health indicators.

• Women's Health Nursing
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• v.18 no.4
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• pp.290-301
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• 2012

Purpose: The purpose of this study was to investigate the contraceptive knowledge and practice among married immigrant women. Methods: A cross-sectional survey design with a convenient sampling was used. 170 married immigrant women were recruited in Daejeon city and Chungcheong area. A structured questionnaire was self-administered from November 1, 2011 to January 13, 2012. Results: 48.2% of subjects have used contraceptive practice. The widely used contraception methods were IUD, condom, oral pill and withdrawal. 73.2% of subjects got information about contraception from family and friends, and 18.3% of subjects had no source of information. The subjects' employment state (p = .006), duration of marriage (p = .019), number of baby (p = .021), family type (p = .046) have a significant influence on their contraceptive practice. The subjects' mean score of contraceptive knowledge was very low (5.72 points out of 15 points). There were significant differences in subjects' contraceptive knowledge depending on their education level (p = .002), employment state (p<.001), country of origin (p = .010), and family type (p = .003). Conclusion: To improve contraceptive knowledge and practice for married immigrant women, it is necessary to develop a proper contraceptive education program to enhance contraceptive knowledge and practice. Adjusted education program by this results will contribute to increase sexual health for married immigrant women.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.1
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• pp.165-170
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• 2016

Background: Nurses are the most visible, frontline personnel providing health education to patients. In particular, nurse experience with Pap examinations have the potential to influence women's attitudes toward screening for cervical cancer. However, nurses in Taiwan have lower rates of Pap testing than the general population. Understanding the factors predicting nurse intent to have a Pap exam and Pap exam status would inform interventions and policies to increase their Pap exam uptake. Therefore, the present study was undertaken. Materials and Methods: Data were collected by questionnaire from a convenient sample of 504 nurses at a regional hospital in central Taiwan between August and October 2011 and analyzed by descriptive statistics, confirmatory factor analysis, and logistic regression. Results: Nurse intention to have a Pap exam was predicted by younger age, less negative attitudes toward Pap exams, and greater influence of others recommendations. However, nurses were more likely to actually have had a Pap exam if they were older, married, had sexual experience, and had a high intention to have a Pap exam. Conclusions: Nurses who are younger than 34 years old, unmarried, sexually inexperienced, and with low intention to have a Pap exam should be targeted with interventions to educate them not only about the importance of Pap exams in detecting cervical cancer, but also about strategies to decrease pain and embarrassment during exams. Nurses with less negative attitudes and experiences related to Pap exams would serve as role models to persuade women to have Pap exams, thus increasing the uptake rate of Pap exams in Taiwan.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1427-1430
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• 2015

Aim: To compare prevalence of anxiety in women with abnormal cervical cytology (Pap) undergoing colposcopy to that of women attending the outpatient clinic for check-up and to examine predicting factors. Materials and Methods: In this cross-sectional analytical study, 100 women with abnormal cervical cytology (abnormal Pap group) and 100 women who attended our outpatient clinic for check-up (control group) were recruited from June 2013 to January 2014. The Hospital Anxiety and Depression Scale (HADS) was employed to determine anxiety in the participants with the score of ${\geq}11$ suggestive of clinically significant anxiety. The prevalence of anxiety and the mean HADS scores for anxiety were compared between the groups. For those with abnormal Pap, association between clinical factors and anxiety was assessed. A p-value of < 0.05 was considered significant. Results: Median age was different between the groups, 44.0 years in the abnormal Pap group and 50.0 years in the control group (p=0.01). The proportion of participants who had more than one sexual partner was higher in the abnormal Pap group, 39.2% vs. 24.7% (p=0.03) and the prevalence of anxiety was significantly higher 14/100 (14.0%) vs. 3/100 (3.0%) (p < 0.01). The prevalence of depression was comparable between the groups. The mean HADS scores for anxiety and depression subscales were significantly higher in the abnormal Pap group, 6.6 vs. 4.8 (P < 0.01) and 3.9 vs. 3.1 (p=0.05), respectively. For the abnormal Pap group, no definite association between clinical factors and anxiety was demonstrated. Conclusions: The prevalence of anxiety in women with abnormal Pap awaiting colposcopy was significantly higher than that of normal controls. Special attention including thorough counselling, with use of information leaflets and psychological support, should be directed to these women.

• Women's Health Nursing
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• v.25 no.4
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• pp.446-458
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• 2019

Purpose: University students happen to be in a transitional period at the beginning of one's adult life and thereby establish the basis for their health care. The negative lifestyles followed by students during this period can also affect their reproductive health. The purpose of this study was to identify lifestyle, reproductive health, gender differences and relationships between lifestyle and reproductive health in university students. Methods: We used a descriptive cross-sectional design. A total of 300 subjects were enrolled. Data were collected using structured questionnaires between October 11 and 25, 2017 and analyzed using SPSS 25.0. Subjects agreed to undergo a face-to-face interview, including administration of the Health Promotion Lifestyle Profile II (HPLP-II) and reproductive health (knowledge, attitude, and behaviors). Results: The mean age of the subjects was 21.4 years. HPLP-II and reproductive health behaviors were significantly different between the genders. The scores of physical activity and nutrition in females were significantly lower than males. The scores of safe sex and sexual responsibility in females were significantly higher than males, and the score of genital health management was significantly lower in females than males. High HPLP-II score was observed to be in correlation with high reproductive health attitudes and behaviors. Conclusion: The result revealed differences in lifestyle and reproductive health between both the genders. For improvement of reproductive health of university students, provision of lifestyle intervention including healthy nutritional habits and physical activity is imperative.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.31-31
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• 1999

Characterization of mutant mice has been utilized as an animal model for the study of human inherited diseases. In addition to the pathogenesis stduy using the mutant mice, the mice have been used for the identification of the genes causing the phenotypes. Functional cloning and positional cloning are two approaches, depending on the phenotypes of the mutant mice. Though it takes a long time positional cloning has been well used to identify the gene of which function can not be presumed from the mouse phenotype. Recently by the advance of the molecular tools and the human genome project close to 10,000 genetic markers are developed to make the procedure faster. We obtained a new mutant mouse, sims, spontaneously arose and the affected mouse has a mild tremor and seizure was observed. Homozygote in either sex is sterile since uterus growth in female and seminal vesicle in male are not induced for the growth in puberty, implying the abnormal hormonal regulation during puberty. Supporting this, there is no detectable testosterone in the serum of the mutant male and the brain of the mutant is 30% heavier than littermate. To identify the location of the mutated gene, intraspecies cross to CAST/Ei was carried out and the 37 affected mice was analyzed for the linkage. The gene was mapped on chromosome 18, 20 cM from the centromere. More than 500 F2 progenies have been analyzed for the linkage and the locus becomes narrow within 3cM between Egrl and Fgf gene.f gene.