• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.335-339
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• 2014

Cowper chlorotic mottle virus (CCMV) is the 'controlled' quarantine virus as plant pathogenic virus that are classed as group VI (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae, When plants that are Phaseolus vulgaris, Clitoria ternatea, Nicotiana tabaccum, Glycine max, Vigna unguiculata and Vigna siensis, and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field.

• The Korean Journal of Mycology
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• v.20 no.3
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• pp.252-258
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• 1992

Thirty three species and two species varieties belonging to 14 genera of fungi were collected from 20 cowpea cultivars on glucose Czapek's agar (11 genera and 25 species+1 var.) and glucose-Czapek's agar supplemented with 10% NaCl (7 genera and 18 species+2 var.) at $28{\pm}2^{\circ}C$. The total count of fungi were 6716 colonies/g in all cowpea cultivars. On glucose-Czapek's agar and identified; Aspergillus flavus, A. niger, A. sydowii, A. flavus var. columnaris, A. terreus, Penicillium chrysogenum, Emericella nidutans and Rhizopus stolonifer. The total count of halotolerant or halophilic fungi was 3515 colonies/g on 10% NaCl-glueose-Czapek's agar and identified; the most common species were: A. flavus, A. sydowii, A. tamarii A. flavipes, A. niger, A. flavus var. columnaris, A. ochraceus, A. oryzae and P. chrvsogenusm. Thin layer chrormatographic analysis of chloroform extracts of the different seed samples revealed that four cultivars were naturally contaminated with aflatoxins $B_1,\;B_2,\;G_1$ and $G_2$, $(45-112\;{\mu}g/kg)$.

• The Korean Journal of Mycology
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• v.44 no.1
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• pp.61-63
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• 2016

Sclerotium rot disease on cowpea (Vigna sinensis King) was observed in the exhibition field of Gyeongsangnam-do Agricultural Research and Extension Services in September 2015. Lesions were covered by white mycelial mats, and numerous sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1~3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on potato dextrose agar (PDA) was $30^{\circ}C$, with the hyphal width of $4{\sim}8{\mu}m$. For molecular identification, the complete internal transcribed spacer (ITS) rDNA region of the causal fungus was sequenced and analyzed. Based on the mycological characteristics, ITS rDNA sequence analysis, and pathogenicity test, this fungus was identified as Sclerotium rolfsii. This is the first report of sclerotium rot on cowpea caused by S. rolfsii in Korea.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.141.1-141
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• 2003

Forty six isolates of bean common mosaic virus (BCMV) collected from azuki bean, mungbean, kidney bean, cowpea, broad bean and peanut were classified into three groups based on biological, serological, cytopathological, and molecular characteristics. Group I induced vein-banding symptoms in cowpea which was similar to those produced by the BCMV-cowpea strain. Group II caused mosaic symptoms in azuki bean but not in peanut and tobacco. Since this character was different from that of previously described BCMV strain, group II may not belong to BCMV GroupIII induced vein-clearing symptoms in azuki bean, kidney bean and peanut, which are typical symptoms for BCMV-peanut stripe virus strain. Virus inclusion patterns of BCMV groups were similar to those of Potyvirus subdivision III with the scroll, pinwheel and long laminated inclusions. However, the inclusions of laminated aggregates were never observed in mungbean isolates. Multiple alignment as well as cluster dendrograms of 3'noncoding region (3'-NCR) and a part of coat protein gene (CP) suggested that group I belongs to the BCMV-cowpea strain, group II to the BCMV-azuki bean strain, and group III to the BCMV-peanut stripe virus strain. Since molecular phylogenesis of BCMV based on nucleotides of 3'-NCR and coat protein differed from the grouping based on virulence differentiation, and BCMV groups are more closely related to each other with the same host origin, other characteristics of those strains are under investigation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.193-193
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• 2022

Cowpea(Vigna unguiculata L. Walp) has a low fat and protein content and a relatively high starch content, so it is mainly used in various ways for song-pyeon sediment, rice-cake paste, and porridge. In Yeonggwang-gun, Jeollanam-do, cowpea for seed production is being cultivated for the localization of raw material for Rice cake with ramie leave. A cowpea variety 'Jang-Alchan' was improved from the cross between IT145373 and IT145391 at the JARES in 2019. 'Jang-alchan' has an erect plant with an intermediate plant habit, light purple-colored corolla, and heart-shaped leaflets. 'Jang-alchan' has an orange-yellow seed seed-surface of faint luster, and brown and straight pods when matured. The stem length of 'Jang-alchan' was 48cm, 9cm longer than that of the control cultivar 'Okdang' and the 100-seed weight was 14.1g. The weight ratio of the seed coat for the entire seed was 11.2%, which was 0.2%p lower than that of the control cultivar. Field resistance of 'Jang-alchan' to leaf and systemic diseases was similar to that of the control cultivar. Is field resistance of 'Jang-alchan' to lodging was slightly lower than that of the control cultivar. The average yield of 'Jang-alchan' was 1.85ton per hectare, which was 14% higher than that of the control cultivar 'Okdang', 'Jang-Alchan' does not require the installation of an espalier-net and is taller than the existing supplied 'Okdang', so it can be harvested by combine harvesting. In addition, it is expected to contribute to the localization of raw material grains for regional specialties as it can be cultivated over a large area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.332-340
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• 2014

The purpose of this study was to establish the optimal growth temperature and to select genetic resources for production of cowpea sprouts. Seowon was treated between $15^{\circ}C$ and $30^{\circ}C$ at intervals of $3^{\circ}C$ to investigate growth temperature. Twelve resources, including Seowon, IT154149, IT154153, Tvu7426, and Tvu7778, were used for cultivating sprouts at a temperature of $27^{\circ}C$. The yield ratio of cowpea sprouts was highest at $27^{\circ}C$ (657%), and was reduced when growth temperature was decreased. The hard seed rate was lower when the growth temperature was increased. Vitamin C content was highest at $24^{\circ}C$ (2.85 mg/g), ranged between 2.15 and 2.29 mg/g at other growth temperatures, and increased with the length of the growth period. The inorganic component content of cowpea sprouts did not vary based on growth temperature, while the amino acid content increased with increasing growth temperature between $15^{\circ}C$ and $24^{\circ}C$, and then subsequently decreased as growth temperature rose from $24^{\circ}C$ to $30^{\circ}C$. IT154153 had the highest yield ratio of cowpea sprouts per genetic resource (647%), followed by Seowon (615%), and Tvu7426 (608%). Genetic resources with a higher yield ratio had smaller seeds, a thinner seed coat, and superior germinability. The inorganic components found at highest concentrations in the cowpea sprouts were potassium, magnesium, calcium, sodium, iron, molybdenum, and zinc (in that order). In comparison to raw seeds, the protein, calcium, zinc, molybdenum, and iron content in the cowpea sprouts was higher, while the content of aluminum and boron was lower.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.4
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• pp.411-420
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• 1996

Asia Triple HIn this study an attempt was made to determine the cost of producing fodders at Savar Dairy Farm (SDF). For this purpose, all seasonal fodders, such as Maize, Jowar, Cowpea and Oat and all perennial fodders, such as Para and Napier were studied. The highest acreage of land was allocated to high-land Para (33.8%) and the lowest acreage (2.7%) was devoted to Cowpea. Jowar attained the highest yield (74.2 tons) per hectare and Maize attained the lowest yield (25.8 tons/h). The highest cost per hectare was attributed to Jowar (TK. 20944.18) while the lowest cost was attributed to low-land para (TK. 10349.86). The cost of production of fodder per kilogram was the highest (TK. 0.66) for Maize and the lowest for Oat (TK. 0.24). The cost of production of low-land Para was much lower than that of high land Para. The per kilogram cost of silage production was the highest (TK. 0.71) for Maize and the lowest (TK. 0.31) was for Napier. The gross return analysis further showed that the highest net margin and B:C ratio were observed for Napier followed by Low-land Para, Jowar, Oat, Cowpea, High-land Para and Maize. Therefore, those fodders whose B:C ratios and yield/ha were higher should be allocated more area of land to stimulate increased returns to SDF in the future is suggested.

• The Plant Pathology Journal
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• v.19 no.3
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• pp.166-170
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• 2003

Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.4
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• pp.341-343
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• 2001

Molecular markers have become fundamental tools for crop genome study. The objective of this study was to construct a genetic linkage map for cowpea with PCR-based molecular markers. Five hundred and twenty random RAPD primers were screened for parental polymorphism. Ninety RAPD markers from sixty primers was segregated in 75 F2 mapping population derived from the cross of local cultivars GSC01 and GSC02. 70 RAPD markers were found to be genetically linked and formed 11 linkage groups. Linkage map spanned 474.1 cM across all 11 linkage groups. There are six linkage groups of 40 cM or more, and five smaller linkage groups range from 4.9 to 24.8 cM. The average linkage distance between pairs of markers among all linkage groups was 6.87 cM. The number of markers per linkage group ranged from 2 to 32. The longest group 1 spans 190.6 cM, while the length of shortest group 11 is 4.9 cM. This map is further needed to be saturated with the various markers such as RFLP, AFLP, SSR and more various populations and primers. In addition, morphological markers and biochemical markers should be united to construct a comprehensive linkage map.

• Korean Journal of Medicinal Crop Science
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• v.20 no.1
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• pp.42-46
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• 2012

Soy isoflavones have been suggested to improve bone loss and lipid profile in postmenopausal women and ovariectomized rats. In present study, we investigated the hypothesis that consumption of soybean, mung bean, cowpea and azuki bean has a beneficial effect on lipid profile which associates with bone mass in ovariectomized rats. Forty two female Sprague-Dawley rats were either sham-operated (Sham) or surgically ovariectomized (OVX). Sham and OVX groups were fed a regular AIN-93M diet, but ovariectomized rats with soybean (OS), mung bean (OM), cowpea (OC) or adzuki bean (OA) were fed AIN-93M diet replacing 35% of corn starch with powdered OS, OM, OC or OA for 10 weeks. Total- and LDL-cholesterol concentrations were significantly lower in Sham and OC groups than other OVX groups. There was significant negative association between total cholesterol concentration and bone mineral density (BMD) of tibia in only OC group. In conclusion, total-cholesterol concentration was significantly and negatively correlated with BMD in rats consumed cowpea, suggesting that the reduced cholesterol concentration may have a beneficial effect on bone mass.