The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Sclerotium Rot of Cowpea (Vigna sinensis King) Caused by Sclerotium rolfsii

Sclerotium rolfsii에 의한 동부 흰비단병

  • Kwon, Jin-Hyeuk (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Kang, Dong-Wan (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Han, Inyoung (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Choi, Yong-Jo (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Lee, Sang-Dae (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Son, Daeyoung (Department of Plant Medicine, Gyeongsang National University)
  • Received : 2016.02.24
  • Accepted : 2016.03.12
  • Published : 2016.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Sclerotium rot disease on cowpea (Vigna sinensis King) was observed in the exhibition field of Gyeongsangnam-do Agricultural Research and Extension Services in September 2015. Lesions were covered by white mycelial mats, and numerous sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1~3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on potato dextrose agar (PDA) was $30^{\circ}C$, with the hyphal width of $4{\sim}8{\mu}m$. For molecular identification, the complete internal transcribed spacer (ITS) rDNA region of the causal fungus was sequenced and analyzed. Based on the mycological characteristics, ITS rDNA sequence analysis, and pathogenicity test, this fungus was identified as Sclerotium rolfsii. This is the first report of sclerotium rot on cowpea caused by S. rolfsii in Korea.

2015년 9월 경상남도농업기술원 시험연구 포장에서 재배 중인 동부에 흰비단병 증상이 발생하였다. 병징은 동부의 줄기 지제부위가 수침상으로 물러지고 부패되어 서서히 시들면서 포기 전체가 말라 죽었다. 줄기의 병반부와 토양표면에 흰색의 곰팡이가 발생하며 갈색의 둥글고 작은 균핵이 많이 형성되었다. 감자한천배지에서 균총은 흰색으로 잘 자라며 배양기간이 경과함에 따라 갈색의 둥글고 작은 균핵을 많이 형성하였다. 균핵의 크기는 1~3 mm이며 균사의 폭은 $4{\sim}9{\mu}m$였다. 균사생육과 균핵형성 적온은 $30^{\circ}C$이었다. 담자균 균사특유의 clamp connection이 관찰되었다. 동부에서 발생한 병징과 병원균의 균학적 특징, internal transcribed spacer rDNA 염기서열을 비교 분석한 결과를 토대로, 이 병을 Sclerotium rolfsii에 의한 동부 흰비단병으로 명명하고자 제안한다.

Keywords

References

  1. Lee TB. Coloured flora of Korea. Hyangmoonsa: Seoul; 2003.
  2. Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  3. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  4. Mordue JE. Sclerotinia rolfsii. In: Commonwealth Mycological Institute (Great Britain), CAB International, editors. CMI descriptions of pathogenic fungi and bacteria. Wallingford: CAB International;1974. no. 410.
  5. Kwon JH, Lee HS, Kim J, Kim WI, Shim HS, Shen SS. Sclerotium rot of Cyclamen europaeum caused by Sclerotium rolfsii. Res Plant Dis 2014;20:223-6. https://doi.org/10.5423/RPD.2014.20.3.223
  6. Kwon JH, Hwang GC, Lee SB, Choi YJ, Lee SD, Nguyen LM. Sclerotium rot of Pulsatilla koreana Nakai caused by Sclerotium rolfsii. Res Plant Dis 2015;21:334-6. https://doi.org/10.5423/RPD.2015.21.4.334
  7. Adegbite AA, Amusa NA. The major economic field diseases of cowpea in the humid agro-ecologies of South-Western Nigeria. Afr J Biotechnol 2008;7:4706-12.