• 대한본초학회지
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• 제20권3호
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• pp.19-27
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• 2005

Objectives : The antioxidant activities of Yeonkyo-san and its ingredient were investigated for industrial application. Methods : We experimented radical scavenging effect, superoxide dismutase and cytotoxicity effect of various cancer cell. Results : The result were obtained as follows : Primary testing of cytotoxicity of Yeonkyo-san and ingredient was done for G361. B16F10, MDA and A549 cell lines. Yeonkyo-san and ingredient water extract, ethanol extract showed cytotoxicity of over 90% respectively against cell line. The electron donating ability(EDA) of water extract from the Yeonkyo-san was higher than 60%, ethanol extract from the Yeonkyo-san was over 80% at 1000 ppm. The electron donating ability(EDA) of water extract and ethanol extract from the Yeonkyo-san ingredients were increased as well. SOD-like activity was high as 75% in 700 ppm, it was increased. All the other samples showed less than 40% SOD-like activity. Conclusions : The results indicated that water extract and ethanol extract of Yeonkyo-san and ingredient can be used as a natural ingredient with biological functions in cosmetic and food composition.

• 대한본초학회지
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• 제22권3호
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• pp.85-91
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• 2007

Objective : In this study, anti-oxidant and cosmeceutical activities of Isatis tinctoria L. extracted from water, ethanol and supercritical fluid condition were confirmed to investigate cosmeceutical activities for utilization as cosmetic ingredient. Methods: Anti-oxidant and cosmeceutical activities were investigated by using electron donating ability, xanthine oxidase, tyrosinase, astringent effect. Result : Isatis tinctoria L. extracts by supercritical fluid, water and ethanol showed good electron donating ability which were 82.7%, 62.6% and 44.8% at the concentration at 1,000ppm, respectively. Xanthine oxidase activity related with purine metabolism was inhibited by ethanol extract about 52.3% at the concentration at 1,000ppm. Tyrosinase inhibition effects, by supercritical fluid extract, ethanol extract and water extract, were 83.3%, 52.9% and 41.2% respectively at 1,000ppm. In the measurement of astringent effect, supercritical fluid extract at the concentration to 5,000ppm showed 85.7% in related activity. The water extract showed 95.9% nitrite scavenging activity at 5,000ppm. Conclusion : According to these results, it is possible that the extract of Isatis tinctoria L. can be used as a new natural material of cosmetic industry.

• 대한본초학회지
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• 제26권2호
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• pp.67-73
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• 2011

Objectives : The purpose of this research was total polyphenol contents, anti-oxidant activities, anti-inflammatory activities and anti-wrinkle activities of Ulleung islands plants for application as a cosmeceutical ingredients Methods : We were experimented total polyphenol contents, anti-oxidant activities, anti-inflammatory activity and anti-wrinkle activities. Results : In the physiological activities, most Ulleung islands plants is showed the highest anti-oxidant, anti-inflammatory activity in ABTS+ radical cation scavenging activity, hydrogen peroxide($H_2O_2$) scavenging activity, nitric oxide scavenging activity. All experiment of the water and ethanol extract from the Ulleung islands plants ingredients were gradually increased as well. Conclusions : These results suggest that extracts from Ulleung islands plants can be used in natural ingredient in food or cosmetic industry.

• 무역상무연구
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• 제34권
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• pp.189-223
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• 2007

In Korea-U.S.A. FTA Negotiation, U.S.A. request to eliminate barriers of tariffs and non-tariff in trade of cosmetics. Korea applies tariffs of 8% on most cosmetics and personal care products. There are some non-tariffs barriers in cosmetics trade between Korea and U.S.A., for example, transparency on restriction and regulatory, cosmeceuticals, import clearance review, quality control testing, ingredient labelling. Tariffs of 8% on most cosmetics should be eliminated, a proviso of complementary measures on sanitation of Korean people about imported cosmetics. This is a meaning of reformation of prior management(tariffs) by strong ex post management in cosmetics trade. It is important that Korea should construct system of ex post management, for instance, construction of data base on manufacturer, importer, bland name of cosmetics and all ingredients of cosmetics. This is concerned with labelling of cosmetics and cosmeceutical and publication of Korean edition of INCI(International Nomenclature of Cosmetic Ingredient).

• 대한화장품학회지
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• 제33권4호
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• pp.275-280
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• 2007

본 연구는 암대극 추출물의 화장품 원료로서의 특성을 알아보기 위하여 항산화, 미백 및 항염 효과와 관련된 다양한 실험을 실시하였다. 암대극 70 % 메탄올 추출물은 MPLC를 사용하여 5개의 분획들로 분리하였다. 1번과 5번 분획에서 항산화(Mn-SOD 생성 억제), 미백(세포 내 멜라닌 생성 억제) 그리고 항염($IL-1{\alpha}$, IL-6, COX-2, Total NO 생성 억제)효과를 나타내었다. 이러한 결과를 종합해 볼 때, 암대극 추출물은 화장품 원료로서의 개발이 기대된다.

• 대한본초학회지
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• 제20권4호
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• pp.41-51
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• 2005

Objectives : The purpose of this research was physiological activities, cytotoxicity, and antibacterial activities of the Cheongyeolsodokum and its constituent herbs. Methods : Physiological activities, cytotoxicity, and antibacterial activities were examined through the Cheongyeolsodokum and its constituent herbs. Results : In the physiological activities, the electron donating ability(EDA) of the water and ethanol extracts from the Cheongyeolsodokum were over 60% and 80% at 100 ppm, respectively. The EDA of the water and ethanol extract from the Cheongyeolsodokum ingredients were gradually increased as well. Water and ethanol extracts from the Cheongyeolsodokum and its constituent herbs inhibited xanthine oxidase activity, they showed superoxide dismutase(SOD)-like activity. The Cheongyeolsodokum and its constituent herbs inhibited cancer cell growth in a dose-dependant manner. Also, the clear zones against Staphylococcus aureus and S. epidermidis were clear shown at 2.5 and 5 mg/disc. Its constituent herbs showed the clear zone against various bacteria such as Candida albicans, S. aureus, S. epidermidis, and S. mutans were shown at 0.5 and 1mg/disc. Conclusions : We observed physiological activities, cytotoxicity, and antibacterial activities of the Cheongyeolsodokum and its constituent herbs. The results also indicated that water extract and ethanol extract of Cheongyeolsodokum and its constituent herbs can be used as a natural ingredient in food or cosmetic industry.

• 대한본초학회지
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• 제20권3호
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• pp.51-58
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• 2005

Objectives : The purpose of this research was physiological activities and investigate cytotoxicity of Sunbanghwalmyung-um extract. Methods : Physiological activity and a cytotoxicity were examined through the hot water and ethanol extracts from Sunbanghwalmyung-um and its ingredient. Results : The electron donating ability(EDA) was 66.9%, 71.3% in 1000 ppm water extract and ethanol extract. Above 90% from 1000 ppm ethanol extract showed a higher activities and it is Rhei Radix et Rhizoma, Lonicerae Flos, Gleditsiae Spina, Glycyrrhizae Radix, Myrrh. SOD-like activity was weak as 12.24%, 16.62% in 700 ppm. In water and ethanol extracts cytotoxicity were against G361, B16F10, MDA, A549, high cytotoxity over 70%. Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Paeoniae Radix Rubra showed high cytotoxicity in water and ethanol extracts. Conclusions : We observed physiological activities and investigated cytotoxicity of Sunbanghwalmyung-um and its ingredients. The results also demonstrated in food or cosmetic industry.

• 한국자원식물학회지
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• 제22권4호
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• pp.343-348
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• 2009

음나무 잎 추출물의 생리활성을 분석하기 위해 열수추출물과 70% EtOH 추출물의 폴리페놀의 함량을 측정하였으며, 항산화활성 분석을 위해 전자공여능, nitric oxide radical 저해활성, xanthine oxidase 저해활성, ABTS radical cation decolorization을 측정하였다. 폴리페놀의 총 함량은 열수추출물과 70% EtOH 추출물에서 각각 104 mg/L, 125 mg/L으로 정량되어 70% EtOH 추출물이 열수추출 물에 비해 높은 함량을 보였다. DPPH radical 소거능은 70% EtOH 추출물이 1,000 ppm에서 93.1%로 나타나 같은 농도의 BHA와 유사한 소거능을 보였다. nitric oxide radical 저해활성은 70% EtOH 추출물과 열수추출물이 1,000 ppm에서 49.6%, 49.5%로 나타나 같은 농도의 BHA보다 높은 nitrit oxide radical 저해활성을 보였다. Xanthine oxidase 저해활성은 1,000 ppm농도에서 열수 추출물과 70% EtOH 추출물에서 각각 46.6%, 60.4% 활성을 나타내었다. ABTS radical cation decolorization 활성은 1,000 ppm에서 열수추출물이 92.7%, 70% EtOH 추출물이 91.4%로 나타났다. 이상의 결과로 보아 음나무 잎의 70% EtOH 추출물과 열수추출물 모두 많은 폴리페놀이 함유되어 있으며, 70% EtOH 추출물과 열수추출물 모두 합성항산화제에 비해 항산화 활성이 다소 낮지만 천연 항 산화제로 우수한 항산화활성을 나타내는 것으로 분석되었다. 이를 바탕으로 음나무 잎의 강한 항산화능을 피부노화 예방관련 미용식품 및 기능성 화장품 소재로 활용 가능한 유용한 수종으로 개발 될 수 있을 것이다.

• 한국산림과학회지
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• 제97권1호
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• pp.88-94
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• 2008

본 연구는 리기다소나무 수피의 화장품 약리활성인 항산화활성을 검증하고, 화장품 산업적 응용을 위하여 리기다소나무 수피 추출물의 화장품 천연소재로써 적용 가능성을 확인하였다. 페놀화합물은 물가용부와 EtOAc가용부에서 각각 47mg/L, 601mg/L로 정량되어 EtOAc가용부가 물가용부에 비해 12배 높은 함량이었다. DPPH 소거능은 EtOAC가용부 10ppm에서 86%로 나타나 같은 농도의 BHA보다 높은 소거능을 보였다. Xanthine oxidase 저해활성은 500ppm농도에서 물가용부와 EtOAC가용부에서 각각 76.3%, 80.5%로 70% 이상의 활성을 나타내었다. Tyrosinase 저해활성은 시료의 농도가 증가함에 따라 유의적으로 증가하는 경향을 나타내었으나, 전체적인 활성은 1,000ppm에서 물가용부가 42%, EtOAC가용부가 10.9%로 나타내었다. 이상의 결과로 미루어 보아 리기다소나무 수피 추출물의 항산화효과가 있음을 확인할 수 있었고, 기능성 화장품 소재로써 이용가능성을 확인할 수 있었다.

• Applied Biological Chemistry
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• 제48권3호
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• pp.273-279
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• 2005

To develop cosmetics using Jindalae flowers (Rhododendron mucronulatum), the surface tensions of extracts were measured and the properties and stability of cream with extracts were investigated. The surface tension of 0.1% ethanol extract was 30.42 mN/m and that of distilled water was 72.2 mN/m. The surface tension of cream with 0.1% ethanol extract was similar to that of sample cream and the measured pH were weakly alkalic. The surface tension of 1% ethanol extract was the lowest value of 24.98 mN/m, the measured pH of cream with 1% ethanol extract was weakly acidic and the particle size of cream was stable. According to an oscillatory test, linear viscoelastic region was extended by adding of 1% water extract and 1% ethanol extract to cream, indicating that the cream had greater enhanced resistance for preserving inner structure as compared to outside stress.　Besides, as a result of the diminished loss angle of ethanol extract cream, the elasticity of cream was increased more than that of sample cream and cream with 0.1% ethanol extract. In contrast, in the case of the increased loss angle of water extract cream, the viscosity of cream was increased. In conclusion, Rhododendron mucronulatum can be deliberated as a cosmetic material because 0.1% water and ethanol extracts showed efficacious physiological activities and cream with 1% extracts could extend linear viscoelastic region.