Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Study on the Anti-oxidant Effect of Pinus rigida Mill. inner Bark Extracts

리기다소나무(Pinus rigida Mill.) 내수피 추출물의 항산화효과에 관한 연구

  • Jang, Min-Jung (Department of Cosmeceutical Science, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Chang-Eeon (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Byung-Guen (Department of Forest Resources, Yeungnam University) ;
  • Lee, Do-Hyung (Department of Forest Resources, Yeungnam University)
  • 장민정 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 이창언 (대구한의대학교 화장품약리학과) ;
  • 이진태 (대구한의대학교 화장품약리학과) ;
  • 이병근 (영남대학교 산림자원학과) ;
  • 이도형 (영남대학교 산림자원학과)
  • Received : 2008.01.23
  • Accepted : 2008.03.03
  • Published : 2008.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of the study was to assess the cosmeceutical activity of Pinus rigida Mill. and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. The concentration of total phenolic compound of P. rigida water soluble fraction and P. rigida ethyl acetate soluble fraction showed 47 mg/L and 601 mg/L respectively. In the result of DPPH scavenging radical activity, P. rigida ethyl acetate soluble fraction showed 86% and it was similar to BHA effect at 10 ppm concentration. Xanthine oxidase inhibition of P. rigida water soluble fraction and P. rigida ethyl acetate soluble fraction were 76.3% and 80.5% at 500 ppm, respectively. In the result of tyrosinase inhibition effect related to skin-whitening, P. rigida water and ethyl acetate soluble fraction showed 42%, 10.9% at a 1,000 ppm. In these results, P. rigida has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.

본 연구는 리기다소나무 수피의 화장품 약리활성인 항산화활성을 검증하고, 화장품 산업적 응용을 위하여 리기다소나무 수피 추출물의 화장품 천연소재로써 적용 가능성을 확인하였다. 페놀화합물은 물가용부와 EtOAc가용부에서 각각 47mg/L, 601mg/L로 정량되어 EtOAc가용부가 물가용부에 비해 12배 높은 함량이었다. DPPH 소거능은 EtOAC가용부 10ppm에서 86%로 나타나 같은 농도의 BHA보다 높은 소거능을 보였다. Xanthine oxidase 저해활성은 500ppm농도에서 물가용부와 EtOAC가용부에서 각각 76.3%, 80.5%로 70% 이상의 활성을 나타내었다. Tyrosinase 저해활성은 시료의 농도가 증가함에 따라 유의적으로 증가하는 경향을 나타내었으나, 전체적인 활성은 1,000ppm에서 물가용부가 42%, EtOAC가용부가 10.9%로 나타내었다. 이상의 결과로 미루어 보아 리기다소나무 수피 추출물의 항산화효과가 있음을 확인할 수 있었고, 기능성 화장품 소재로써 이용가능성을 확인할 수 있었다.

Keywords

Acknowledgement

Supported by : 산업자원부

References

  1. 고경식. 1991. 한국식물검색도감(수목). 아카데미서적. pp. 168
  2. 국립산림과학원 편찬위원회. 2006. 임목육종 50년. 국립산림과학원. pp. 459
  3. 배기환. 2000. 한국의 약용식물. 교학사. pp. 34
  4. 奧田拓男. 1987. 植物性分の收斂作用, フレグランス ジャ-ナル, p.270-274, No.6, 1986 Res. 47: 936-942
  5. Araghi-Niknam, M., Hosseini, S., Larson., D., Rohdewald, P. and Watson, R. 2000. Pine bark extract reduces platelet aggregation. Integrative Medicine. 2: 73-77 https://doi.org/10.1016/S1096-2190(00)00002-0
  6. Bauer, G. 2000. Reactive oxygen and nitrogen species : Efficient, selective and interactive signals during intercellular induction of apoptosis. Anticancer Research. 20: 4115-4139
  7. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical, Nature. 181: 1199-1200 https://doi.org/10.1038/1811199a0
  8. Brand-Wiliams, W., Cuvelier, M.E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Technology. 28: 25-30
  9. Brune, B., Zhou, J. and Von, Knethen, A. 2003. Nitric oxide, oxidative stress and apoptosis. Kidney International. 8: 22-24
  10. Cheshier, J., Ardestani-Kaboudanian, S., Liang, B. Araghiniknam, M., Sangbun, C., Lane, L., Castro, A. and Watson, R. 1995. Immunomodulation by pycnogenol in retrovirusinfected or ethanol-fed mice, Life Sciences. 58: 87-96 https://doi.org/10.1016/0024-3205(95)02303-8
  11. Donnelly, J.K., McLellan, K.M., Walker, J.L. and D.S. Robinson. 1989. Superoxide dismutase in foods. Food Chemistry. 33: 243-270 https://doi.org/10.1016/0308-8146(89)90036-8
  12. Hatano, T., Yasuhara, T., Fukuda, T., Noro, T. and T. Okuda. 1989. Phenolic constituents of licoriceII. Structures of licopyranocoumarin, licoarylcoumarin and glisoflavone, and inhibitory effects of licorice phenolics on xanthine oxidase. Chemical & Phamaceutical Bulletin. 37: 3005-3009 https://doi.org/10.1248/cpb.37.3005
  13. Hosseini, S., Lee, J., Sepulveda, R., Rohdewail, P. and Watson, R. 2001. A randomized, double-blind, placebocontrolled, prospective, 16 week crossover study to determine the role of Pycnogenol in modifying blood pressure in mildly hypertensive patients. Nutrition Research, 21: 1251-1260 https://doi.org/10.1016/S0271-5317(01)00342-6
  14. Hwang, H.S., Lee, Y.K. and Lee, K.G. 2006. Fractionation of Banaba Leaves Extract (Lagerstroemia speciosa L. Pers) and its Antioxidant Activity. Food Engineering Progress. 10: 120-124
  15. Jung, M.S, Lee, G.S. and Chae, H.J. 2004 In vitro biological activity assay of ethanol extract of Radish. Journal of Korean Society for Applied Biological Chemistry. 47(1): 67-71
  16. Kelley, W.N. and Wyngaarden, J.B. 1974. Enzymology of gout. Advances in Enzymology and Related Areas of Molecular Biology. 41: 1-33 https://doi.org/10.1002/9780470122860.ch1
  17. Kong, W.S. 2004. Species Composition and Distribution of Native Korean Conifers. Korean J. Geographical Society. 39: 528-543
  18. Korner, A. and Pawelek, J. 1982. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin. Science. 217: 1163-1165 https://doi.org/10.1126/science.6810464
  19. Korycka-Dahi, M., Richardson, T. and Hicks, C.L. 1979. Superoxide dismutase activity in bovine milk serum. Journal of Food Protection. 42: 867-871 https://doi.org/10.4315/0362-028X-42.11.867
  20. Laskin, J.D. and Piccinini, L.A. 1986. Tyrosinase isozyme heterogeneity in differentiating B-16/C3 melanoma. Journal of Biological Chemistry. 261: 16626-16635
  21. Lee, J.T., Jeong, Y.S. and An, B.J. 2002. Physiological activity of Salicornia herbacea and its application for cosmetic materials. Korea Journal of Herbology. 17: 51-60
  22. Lee, S.Y., Hwang, E.J., Kim, G.H., Choi, Y.B., Lim, C.Y. and Kim, S.M. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of Camellia japonica L. Korea Journal of Food Science and Technology. 13(3): 93-100
  23. Lerner, A.B. and Fitzpatrick, T.B. 1950. Biochemistry of melanin formation. Physiological reviews. 30: 91-126 https://doi.org/10.1152/physrev.1950.30.1.91
  24. Marcocci, L., Maguire, J.J., Droylefaix, M.T. and Packer, L. 1994. The Nitric Oxide Scavenging Properties of Ginkgo biloba Extract EGb 761. Biochemical and Biophysical Research Communications. 201, 15: 748-755 https://doi.org/10.1006/bbrc.1994.1764
  25. Marklund, S. and Marklund, G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 47: 468
  26. Packer, L., Rimbach, G. and Virgili, F 1999. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus martima) bark, pycnogenol. Free Radical Biology and Medicine. 27: 704-724 https://doi.org/10.1016/S0891-5849(99)00090-8
  27. Putter, M., Grotemeyer, K., Wurthwein, G. Araghi-Niknam, M., Watson, R., Hosseini, S. and Rohdewald, P. 1999. Inhibition of Smoking-Induced Platelet Aggregation by Aspirin and Pycnogenol. Thrombosis Research. 95: 155-161 https://doi.org/10.1016/S0049-3848(99)00030-4
  28. Stirpe, F. and Corte, E.D. 1969. The regulation of rat liver xanthine oxidase. Journal of Biochemistry. 244: 3855-3861
  29. Storch, J. and Ferber, E. 1988. Detergent-amplified chemiluminescence of lucigenin for determination of superoxide anion production by NADPH oxidase and xanthine oxidase. Analytical Biochemistry. 169(2): 262-267 https://doi.org/10.1016/0003-2697(88)90283-7
  30. Swain, T., Hillis, W.E. and Ortega, M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. Science of Food Agriculture. 10: 83-88
  31. Swan, G.A. 1974. Structure, chemistry, and biosynthesis of the melanins. Fortschritte Chemie Organischer Naturstoffe. 31: 521-582
  32. Wyngaarden, J.B. and Jr. Holmes, E.W. 1977. Molecular nature of enzyme regulation in purine biosynthesis. Ciba Foundation Symposium. 48: 43-64
  33. Yagi, A., Kanbara T. and N. Morinobu. 1986. The effect of tyrosinase inhibition for aloe. Planta Medica, 3981: 517-519
  34. Yoon, S.O., Yoo, S.J. and Oh, J.S. 1997. A study on the pharmacological and nutritional values of needs of Pinus densiflora S. et Z. Research Bulletin of the Eyperiment Foresta. 5: 63-81