• Korean Journal of Microbiology
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• v.49 no.1
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• pp.99-104
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• 2013

Bacteriophage P2 sir mutants are inefficient helpers for their satellite bacteriophage P4. The term, "P2 sir-associated helper inefficiency" has been used to define this phenomenon and it has been suggested that the DNA sequence difference between the cos region of P2 and that of P4 is responsible. To test this hypothesis, P4 derivative phage, P4 sid71 cosP2, containing the cos region of P2 and sid71 allele was constructed through several in vitro DNA manipulation steps. Its burst size was determined using a one-step growth experiment. The results showed that the substitution of the cos region of P2 for the cos region of P4 in P4 sid71 cosP2 overcame "P2 sir-associated helper inefficiency". P4 sid71 cosP2 stock phages prepared with P2 wild type helper and P2 sir helper were analyzed using a CsCl buoyant equilibrium density gradient experiment. The results revealed that the phage particles containing three copies of the P4 genome were the predominant particles in both cases.

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.169-175
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• 1989

To study the role of mutant precore region in expression and secretion of hapatitis B viral core antigen, we have cloned core antigen gene(HBc) with or without precore region in geterologous expression vectors containing SV40 promoter, yeast promoter, and lambda $P_{L}$ promoter. In COS cells transfected with plasmid containing C-gene with precore region, antigens were detected in both cell extract and cultured medium. However, in the cells transfected with plasmids containing C-gene without precore or with mutated precore region by one nucleotide (T) addition at the nucleotide 1,821, HBcAg was detected only in cell extracts. These results support that the mutation by one nucleotide addition shifted the initiation codon of precore region to 53 nucleotides upward and the elongated precore region also played a major role in the secretion of HBcAg in mammalian cells. In the case of yeast and E. coli, HBcAg was detected only in cell extracts in spite of the presence of precore region, which suggest that precore region could not affect HBcAg secretion in these system.

• Journal of the Korean Society of Safety
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• v.15 no.2
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• pp.13-21
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• 2000

Under the mixed mode loading, it has been known that the influence of non-singular terms in the stress fields is serious, because the critical distance, ${\gamma}_o$ - the distance of nonlinear elastic region near the crack tip has no negligible value. In this paper, it has been studied on the influence of non-singular terms, T-stress, and the case of T=0, ${\sigma}cos^2{\beta}$, ${\sigma}cos(2{\beta})$ and $0.05{\sigma}cos(2{\beta})$, under uniaxial tension and compression. It has been seen that the T-stress of ${\sigma}cos(2{\beta})$ has better than others as comparing to the experimental data.

• Korean Journal of Microbiology
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• v.51 no.1
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• pp.1-6
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• 2015

The term "P2 sir-associated helper inefficiency" has been used to define the inefficient helper capability of P2 sir mutants for their satellite bacteriophage P4. The aim of this study was to investigate the factors overcoming P2 sir-associated helper inefficiency. At first, we verified whether the P2 cos region containing P4 sid71 cosP2 could overcome P2 sir-associated helper inefficiency with P2 sir3. The result was that P4 sid71 cosP2 could not overcome P2 sir-associated helper inefficiency with P2 sir3. Instead of cos region of P2, the size of the DNA packaged into a $P2_{sir}$-sized head seems to be important for overcoming P2 sir-associated helper inefficiency. In the present work, three kinds of P4 derivatives with packaged DNA sizes between those of P4 ost1 and P4 ost2, were constructed through DNA manipulation. In one P4 derivative, P4 sid71 delRI::apr, the size of the packaged DNA was identified with a CsCl buoyant equilibrium density gradient experiment. According to the burst sizes of the P4 derivatives, they could overcome P2 sir3-associated helper inefficiency. The size of the P4 derivative DNA suitable for packaging into a $P2_{sir3}$-sized head was 28-29 kb.

• 한국해양학회지
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• v.5 no.1
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• pp.6-13
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• 1970

Ten days and monthly mean temperatures were analysed daily data observed during July, 1916 to March, 1970 statistically. Periodic characters were calculated by Δn, new method of approximate solution of Schuster Method. According to ten days mean temperatures, annual variation function is F($\theta_d$)=16.29-5.27 cos $\theta_d$+0.75 cos2 $\theta_d$-3.14 sin $\theta_d$+1.16 sin2 $\theta_d$-0.63 sin $\3{theta}_d$, where $\theta_d$=$-\frac{\pi}{18}$(d-3), d is the order of ten days period, 1 to 36. Annual mean water temperature is 16.3$^{\circ}C$, minimum in the last ten days of February 10.9$^{\circ}C$, maximum in the last ten days of August 24.5$^{\circ}C$. Periodic character of secular variation shows 11 year and its curve is F($\theta_y$)=16.29+0.53 cos $\theta_y$ -0.16cos $2{\theta}_y$+0.10 cos$3{\theta}_y$-0.10 sin $\theta_y$, where $\theta_y$=2$-\frac{2\pi}{11}$(y-1920), y is calendar year. And the relation between air temperature x and water temprature y is following. y=9.67 1.035$\^x$

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.234-241
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• 2001

The C-terminal 393 bp region of the human interleukin-16 (IL-16) gene was cloned and expressed in E. coli along with mammalian cell lines. Recombinant IL-16 expressed from E. coli was 22 kDa on SDS-PAGE and showed 260% of chemoattractant activity at a concentration of $0.1\;{\mu}g/ml$. HeLa, COS, and Neuro-2a cells were transduced by recombinant retrovirus vector pLNC/IL-16/IRES/TK and the intracellular and secreted amounts of IL-16 produced by HeLa/IL-16/TK, COS/IL-16/TK, and Neuro-2a/IL-16/TK cells were determined by enzyme-linked immunosorbent assay (ELISA). HeLa/IL-16/TK $(1{\times}10^5)$ and COS/IL-16/TK $(1{\times}10^5)$ cells secreted 36.1 and 13.3 ng of IL-16 for 48 h, respectively. Forty-nine ng and 86.4 ng of IL-16 remained in the cell lysates of HeLa/IL-16/TK and COS/IL-16/TK. Intracellular and secreted amounts of IL-16 from Neuro-2a/IL-16/TK $(5{\times}10^5)$ cells during 24 h cultivation were 50 ng and 3.3 ng, respectively. Also, HeLa and COS cells wee stably transfected with mammalian expression vector pCRIII/IL-16. Both culture media and cell lysates prepared from HeLa/IL-16 cells and COS/IL-16 cells showed chemoattractant activity ranging from 190% to 460% as compared to the control experiment. Expression of the herpes simplex virus thymidine kinase (HSV0tk) gene in pLNC/IL-16/ IRES/TK bicistronic retroviral expression vector was verified by performing a genciclovir (GCV) sensitivity assay. Finally, IL-16 repressed Tat-transactivated human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR) promoter activity.

• Bulletin of the Korean Chemical Society
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• v.17 no.5
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• pp.461-463
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• 1996

The laser-induced fluorescence excitation spectrum of 7-amino-4-(trifluoromethyl)coumarin in a supersonic jet has been recorded in the 340-352 nm region. The electronic band origin was observed at 28622.8 cm-1. Vibrational assignments for the three fundamental low-frequency modes and eight combination bands have been made for the S1 electronic excited state. The out-of-plane vibrations of this molecule have been characterized from the low-frequency assignments of the spectrum. The periodic potential energy function for the CF3 torsion, which satisfactorily fits the observed data, were also determined to be V(Φ)=95X(1-cos3Φ)-32X(1-cos6Φ) where Φ is the torsional angle. The relatively low torsional barrier of 99 cm-1 in S1 state could be explained by the small steric interactions between the functional groups attached to a bicyclic ring.

• 한국해양학회지
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• v.5 no.2
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• pp.41-51
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• 1970

Periodic characters of water temperature in the regions of the Mishima and the Okinoshima were derived through the analysis of the five days interval data during 1914 to 1970 mainly. In terms of ten days mean temperatures, annual variation function of the Mishima region, Korea Strait, is F($\theta_d$)=17.45-5.34 cos $\theta_d$-3.77 sin $\theta_d$+0.62 sin $2\theta_d$ -0.52 sin $3\theta_d$, where $\theta_d$=$\frac{\pi}{18}$(d-2), d is the order of ten days period 1 to 36. And in the region of Okinoshima, Tsushima Strait, we find F($\theta_d$)=18.88-5.39 cos $\theta_d$-3.60 sin $\theta_d$+0.52 sin $2\theta_d$. The annual mean temperature is 17.4$^{\circ}C$ in the Mishima region, 18.9$^{\circ}C$ in the Okinoshima region, and the amplitudes of annual variation functions are 7$^{\circ}C$ in both regions with minimum temperature in the middle ten days of February, maximum in the middle ten days of August. The long term variations of surface water temperature with 12 5 years period were observed in the annual mean temperature, monthly mean temperatures and the fixed day temperatures of every year. In addition to these, relatively short term variations were also found significant periods of 3 years, 4 years and 2 years, respectively.

• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1114-1120
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• 2010

A chitosan-degrading fungus, BSF114, was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at an optimum pH of 4.0 and optimum temperature of $60^{\circ}C$ after 36-40 h fermentation. The rapid decrease in the viscosity of the chitosan solution early in the reaction suggested an endo-type cleavage of the polymeric chitosan chains. To identify the isolated fungus, molecular biological and morphological methods were used. The fungal internal transcribed spacer (ITS) region 1 was amplified, sequenced, and then compared with related sequences in the GenBank database using BLAST. The phylogenetic relationships were then analyzed, and the results showed that the fungus belongs to Aspergillus fumigatus. Morphological observations were also used to confirm the above conclusion. The chitooligosaccharides (COS) obtained through hydrolyzing the colloidal chitosan showed that A. fumigatus BSF114 is suitable for degrading chitosan and producing chitooligosaccharides on a large scale. High concentrations of the COS (1,000 and 500 ${\mu}g/ml$) significantly proliferated mice marrow cells.

• Molecules and Cells
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• v.24 no.1
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• pp.148-151
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• 2007

Porcine endogenous retroviruses (PERVs) in the pig genome represent a potential risk of infection in pig-to-human transplantation and are transmitted vertically. The solitary long terminal repeat (LTR) elements of the PERVs affect the replication properties of the individual viruses via their repeat sequences and by encoding a set of specific transcription factors. We examined the promoter activities of solitary LTR elements belonging to the PERV-A and -B families of the Korean domestic pig (KDP) using luciferase reporters. Three of the LTR structures (of PERV-A5-KDP, PERV-A7-KDP, PERV-A8-KDP) had different promoter activities in human HCT116 cells and monkey Cos7 cells, and potential negatively and positively acting regions affecting transcription were identified by deletion analysis. These data suggest that specific sequences in the U3 region of a given LTR element can affect the activities of promoter or enhancer elements in the PERV.