• 한국임상수의학회지
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• 제15권1호
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• pp.1-7
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• 1998

The present study was performed to evaluate circadian rhythm of blood nortisol concentration and change of blood cortisol concentration by various stress type in domestic cats. The ELISA kit for cortisol measurement was evaluated on the effective assay range, day to day variation, within-run variation and accuracy. The results were 1-1,000 ng/ ml, 0.7-5.9%, 0.9-4.5% and 98%, respectively. The circadian rhythm of serum cortisol concentration is presented in cats. Change of serum cortisol concentration was also examined with several stressors, stimulation with hamster as a prey, beep electronic sounds, vehicle transportation and unfamiliar environment. Unfamiliar environment showed most remarkable change in serum cortisol level.

• Safety and Health at Work
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• 제10권1호
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• pp.109-113
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• 2019

Background: In both developed and developing countries, noise is regarded as the most common occupational hazard in various industries. The present study aimed to examine the effect of sound pressure level (SPL) on serum cortisol concentration in three different times during the night shift. Methods: This case-control study was conducted among 75 workers of an industrial and mining firm in 2017. The participants were assigned to one of the three groups (one control and two case groups), with an equal number of workers (25 participants) in each group. Following the ISO 9612 standard, dosimetry was adopted to evaluate equivalent SPL using a TES-1345 dosimeter. The influence of SPL on serum cortisol concentration was measured during the night shift. The serum cortisol concentration was measured using a radioimmunoassay (RIA) test in the laboratory. Repeated measure analysis of variance and linear mixed models were used with ${\alpha}=0.05$. Results: The results indicated a downward trend in the serum cortisol concentration of the three groups during the night shift. Both SPL and exposure time significantly affected cortisol concentration (p < 0.0001, p < 0.0001). Conversely, age and body mass index had no significant influence on cortisol concentration (p = 0.360, p = 0.62). Conclusion: Based on the obtained results, increasing SPL will lead to enhancement of serum cortisol concentration. Given that cortisol concentration varies while workers are exposed to different SPLs, this hormone can be used as a biomarker to study the effect of noise-induced stress.

• Clinical and Experimental Pediatrics
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• 제46권2호
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• pp.183-187
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• 2003

목 적 : 태아시기의 장기간 스트레스는 부신의 호르몬 합성에 영향을 미치는 것으로 알려져 있다. 분만 동안의 급성 스트레스와 제대혈 코티솔간의 상관관계를 보고자 본 연구를 시행하였다. 방 법 : 총 58명의 신생아를 대상으로 분만 즉시 제대혈을 10 mL 채취하였고, $4^{\circ}C$에서 2,000 rpm으로 20분간 원심 분리하여 혈청을 분리한 후, 즉시 $-70^{\circ}C$에 냉동 보관하였다. 코티솔의 측정은 Coat-A-Count Cortisol Kit를 사용하여 RIA방법으로 측정하였다. 분만 스트레스와의 관계를 알아보기 위하여 재태 연령(34주 이하, 35-37주, 38주 이상), 분만방식(제왕절개 분만 vs 질식분만), Apgar 점수, 자궁수축 유무 등에 따른 코티솔 농도를 비교하였다. 결 과 : 제대혈 코티솔 농도는 재태 연령에 따라 증가하는 양상을 보였고(P<0.001), 제왕절개 분만 군보다 질식 분만 군에서 코티솔 농도가 통계적으로 유의하게 높았다(P<0.001). 자궁수축을 동반하는 경우가 동반하지 않았던 경우보다 코티솔 농도가 높았으며(P<0.05), 분만 진통시간이 길수록 코티솔 농도가 유의하게 높았다(P<0.05). 코티솔 농도는 1분 Apgar 점수가 낮았을 때 유의하게 낮은 농도를 보였다(P<0.05). 결 론 : 제대혈 코티솔은 분만스트레스와 밀접한 관련성이 있으며, 신생아의 출생 후 예후와도 관련이 있을 것으로 생각된다.

• 생명과학회지
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• 제14권4호
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• pp.573-576
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• 2004

The purpose of the present study is to investigate the lipid peroxidation, creatine kinase activity and cortisol hormone levels following the training intensity in elite judo players. Six elite Judo players participated in the experiments (3h repetition judo program), which include stretching, judo skill practice and cool down without recess. Blood sampling were taken at the judo gymnasium at the time of resting, 1h training, 2h training, 3h training, 2h recovery, and 24h recovery time and this were analyzed for CK, MDA and Cortisol levels. The results obtained were analyzed via repeated measures of ANOVA using SPSS package program (ver.10.0) and a value of p<.05 was considered statistically significant. The results from this study were as follows. In the CK levels, which reflect the contribution of creatine phosphate and muscle damage degree, there was a significant difference (p<.05) after judo training in every period. Recovery 24h showed the highest level. In the MDA levels, which reflect lipid peroxidation, there was a significant difference (p<.05) after judo training. Recovery 2h showed the lowest level. In the cortisol hormone levels, which reflect stress status, there was a significant difference (p<.05). In this study, we can conclude that For the trained athletes, MDA level was lower at the time of exercise compare to the other period, this is caused by the increased antioxidant defence mechanism.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1297-1302
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• 2003

To determine whether the feeding of different species of hay affects the dry-matter intake and the serum cortisol level of sheep, 6 non-pregnant, non-lactating ewes were offered alfalfa (Medicago sativa) hay (Al), oats (Avena sativa) hay (Ot) and perennial ryegrass (Lolium perenne) hay (Pr) under 6 kinds of treatment including 1) change from Al to Ot, 2) Al to Pr, 3) Ot to Al, 4) Ot to Pr, 5) Pr to Al, and 6) Pr to Ot. The experimental design was a 6${\times}$6 Latin square with a 14 day period of which 9 days were a preliminary period and 5 days as a digestion trial period. The change in hay feeding was done abruptly on the first day of each period without an adaptation period. The blood from the jugular vein was collected on the first, third and the last day of each period through a sterile catheter for the analysis of cortisol. The dry-matter intake was recorded daily throughout the period. The mean daily intake of dry matter (DM, g/kg live weight) was significantly different among the 3 species of hay (Al>Ot>Pr; p<0.05). The digestibility of DM for Al and Ot was the same, but that for Pr was significantly lower than Al and Ot (p<0.05). The mean serum cortisol levels were significantly different among the hays (p<0.05). The level for Pr was the highest and that for Al was the lowest. The abrupt change of hay feeding of 6 treatments produced a significant change in the serum cortisol levels. The DM intake was inversely related to the change of the cortisol level. It is suggested that the animal' intake response to different species of hay may be partly motivated by the psychological feelings toward the hay offered.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.85-85
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• 2019

Objective: In this study, the anti-stress effects of extract of Hydrangeae Dulcis Folium (EHDF) or ethalonic extract of Opuntiaficus-indica (EOF) of natural extracts from Jeju Island were investigated. Methods: We performed measurement of whole-body cortisol level and behavioral experiments including the novel tank test (NTT) or the open field test (OFT) to assess stress responses in zebrafish. To induce physical stress, we used the net handling stress (NHS). Fish were treated with EOF or EHDF for 6 min before they were exposed to stress. And then, we sacrificed fish for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, were evaluated to observe anxiety-like behaviours and locomotion. We used the cortisol enzyme-linked immunoassay kit to measure the amount of cortisol in each zebrafish sample. Results: The results indicate that increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with both EOF and EHDF (P < 0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS. Compared with the NHS-treated stressed control group, pre-treatment with each EHDF and EOF for 6 min significantly prevented the NHS-increased whole-body cortisol level (P < 0.05). Conclusions: In conclusion these results suggest that both EOF and EHDF pretreatment may prevent stress responses and that its mechanism of action may be related to its positive effects on cortisol release.

• Journal of Korean Biological Nursing Science
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• 제12권3호
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• pp.133-139
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• 2010

Purpose: The purpose of this study was to evaluate the effectiveness of aromatherapy on headache, anxiety, and serum cortisol level in middle-aged women with recurrent headaches. Methods: Nineteen patients in the intervention group and 21 in the control group participated in the exercise. They were randomly selected volunteers whose average headache score over 6 months with above 4 points on the visual analogue scale (VAS). The experimental group received aromatherapy for 5 days; inhalation (3 times per day) and an application on the neck and both shoulders (one time per day). Prior and post treatment scores in headache, anxiety, and measurement of serum cortisol in experimental group were measured. Then, these were compared with the control group. The headache score was measured by VAS; anxiety score was measured by State Trait Anxiety Inventory; serum cortisol was measured by Radio-Immune-Assay method. Results: Decrease in headache, anxiety, and serum cortisol level in the experimental group was greater than those in the control group. Conclusion: It confirmed aromatherapy is effective in reducing headache, anxiety, and serum cortisol level.

• Journal of Oral Medicine and Pain
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• 제47권4호
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• pp.183-188
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• 2022

Purpose: The goal of this study was to investigate the causative factors related to the stress of temporomandibular disorders (TMDs) by evaluating salivary cortisol concentration in young adult TMD patients and control groups. Methods: Saliva was obtained from 32 young adult TMDs patients and 34 control patients without a history of TMDs who visited Chosun University Dental Hospital between June 1 and August 31, 2021. Enzyme-linked immunosorbent assay was conducted to measure the salivary cortisol concentration. Results: The salivary cortisol concentration in the TMD patient group and the control group differed significant significantly (p<0.05). The salivary cortisol concentration according to the duration of the clinical symptom of TMD differed significantly difference between the two groups in the male. The salivary cortisol concentration according to perceived stress level differed significantly in the mild and moderate groups (p<0.05). There was no significant difference in salivary cortisol concentration between the two groups related to bruxism or clenching (p>0.05). Conclusions: The salivary cortisol concentration in the TMD patient group and the control group showed statistical relevance, indicating that stress was a causative factor.

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 1972년도 제11차 학술대회
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• pp.71.3-72
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• 1972

• 대한한의학회지
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• 제31권6호
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• pp.21-28
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• 2010

Objective: This study was undertaken to systematically assess and summarize the effects of acupuncture on cortisol secretion. Materials and methods: We searched articles published up to May 2010 in six electronic databases (PubMed, Cochrane Library, KISS, KISTI, DBPIA, Kyobo Scholar). Randomized clinical trials (RCTs) which met all the inclusion criteria were reviewed. Eight RCTs were finally selected for this systematic review and assessed by three reviewers. The risk of bias was also estimated by using the Cochrane criteria. Results: Six RCTs reported no distinct difference of cortisol levels between control and experimental groups. Two RCTs reported significant differences of cortisol levels between groups; one reported the acupuncture group with markedly higher concentrations of cortisol while the other reported the opposite result. Conclusion: There are some difficulties in clearly identifying the effects of acupuncture on cortisol levels in this systematic review due to inconsistent results. Therefore, more rigorous trials with larger scales need to be conducted to clarify the effects of acupuncture on cortisol levels.