• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.21-21
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• 2014

A edible mushroom, Hypsizygus marmoreus is commercially cultivated in Northeast Asia. Japan's annual production is 110,000ton or more. Since 2002, cultivation is expanded in Korea. To investigate the morphological, cultural and microscopic characteristics of Hypsizygus marmoreus, 109 isolates were collected from Korea and other countries. Clamp connection, chlamydospore and arthrospore were present in all tested isolates of H. marmoreus except HYM-002 and HYM-004. Also pilealtrama, gilltrama, basidia, basidiospore and cystidia of fruiting body were no difference among the isolates in the present investigation. Morphological characteristics of fruiting body was that color of pileus was brown and white, irregular as marble, the average size 12~22mm and stipes was $46{\sim}91{\times}6{\sim}10mm$. Isolates HYM-031, HYM-047 and HYM-109 formed grayish-brown pileus with a faint pattern. Molecular analysis with RAPD and ITS rDNA sequence analysis were also performed to check the genetic relationships among H. marmoreus isolates. Based on the RAPD analysis using the URP-PCR, all isolates of H. marmoreus were clustered into large 3 groups but more than 90% showed high similarity. In addition, morphological and geographical differences have been classified as an independent cluster. The brown and white strains enclosed in same cluster. So genetically no significance difference was observed between these two strains. ITS gene sequences of 16 selected isolates which were 640 bp long, were aligned and compared. The similarity in ITS sequence was 94.8 to 99.1% among tested isolates and the H. marmoreus isolates in GeneBank. In conclusion the tested isolates were H. marmoreus. Morphological and molecular observations proved that all tested isolates were belonging to H. marmoreus. For the stable artificial cultivation, composition of optimum media, mature period and light condition were established. Optimal formula of artificial cultivation medium was Douglas sawdust: corn cob: soybean meal: wheat bran = 40:30:15:15. In addition, 7% rice bran and 3% yellow sucrose was the most effective composition for spawn's liquid medium. For the maturation of the isolates was favorable for growing for 20 to 30 days at $25^{\circ}C$ and the LED lights in mixture of white and blue was good for growth period. For effective growth, the temperature, humidity and aeration control in every step was important.

• 인간식물환경학회지
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• 제23권6호
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• pp.615-624
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• 2020

Background and objective: Urbanization caused a reduction in urban green space and a lack of community spaces. An attempt to solve these problems, urban gardens, have become popular and are currently being implemented in various downtown areas. However, urban gardens have some inadequate aspects from a visual or landscape perspective. The goal of this study was to examine the possibility of an aesthetic and productive garden space by introducing cultivation methods through the planting system design process. Methods: The design process using plants was classified and presented to suggest the importance of the landscape and aesthetic value. An urban garden space was designed according to the perspective of 'production scenery' and 'participation aesthetics'. According to the characteristics of the plant, urban gardens were divided into vertical type (corn, millet, sorghum), climbing type (kidney bean, cucumber, bitter gourd), and runner type (melon, watermelon, peanut). After classifying plants according to the shape of the root, the structure supporting climbing was installed and the crops were cultivated in an upright form with a tunnel. Results: In the designed cultivation, each crop cooperates, without invading each other's space. Compared with the conventional cultivation, there was little difference in production, and management was made more convenient since weed outbreaks were effectively suppressed while runner type crops cover over the land's surface. Since the positions of each crop are clearly distinguished, the aesthetic value is improved by offering a sense of rhythm with a balanced design. Conclusion: The results suggest that the cultivation methods through the plant system design process have aesthetic as well as productive value, and the design using plants, an infinite living resource, could lead to an expansion of the design field. Moreover, it would enable a sustainable symbiosis between industry and environment. There is potential for the design industry to make significant progress through collaboration with agriculture, horticulture, and landscape architecture.

• 한국식품영양과학회지
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• 제40권7호
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• pp.962-968
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• 2011

재배 방법이 찰옥수수의 성분과 품질 특성에 미치는 영향을 규명하기 위해, 친환경 및 관행 방법으로 재배된 흑진주, 일미 및 연농1호 품종의 일반성분과 항산화활성을 분석하였다. 조회분은 흑진주, 일미 및 연농1호 모두 재배 방법에 따른 유의적인 차이를 보이지 않았고, 조지방은 흑진주에서는 친환경 재배가, 일미에서는 관행 재배가 유의적으로 높았다. 조단백질은 흑진주에서는 친환경 재배가, 연농1호에서는 관행 재배가 유의적으로 높았다. 아밀로오스 함량은 친환경 재배에서 유의적으로 높았으며, 유리당은 흑진주에서 친환경 재배가 높았다. 아연 함량은 연농1호의 친환경 재배에서 유의적으로 높은 함량을 나타내었으며, 지방산은 연농1호 친환경 재배에서 palmitic acid, stearic acid 및 oleic acid 함량이 높았다. 비타민 E는 일미품종은 친환경, 흑진주와 연농1호는 관행 재배가 높아 품종 간에 차이를 보였다. 총 폴리페놀 함량 및 전자공여능은 흑진주, 일미 및 연농1호 모두에서 친환경 재배보다 관행 재배에서 높게 나타났으며 총 항산화력 에서는 흑진주 관행 재배가 유의적으로 높았고, 일미에서는 친환경 재배에서 유의적으로 높았다. 이상의 결과로 부터 각 품종별로 다른 패턴을 나타내고 있어 일정한 차이를 인정하기 어려우며 따라서 각 품종별 우수한 측정항목을 평가 하여 친환경 재배에 적합한 품종의 선발이 필요하다고 판단된다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.92.2-93
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• 2003

Severe soybean-sprout rot was found at the mass productive factory in 2000 and 2001 and it caused 10-20％ loss of the production. Pythium sp. was isolated almost 90％ by potato dextrose agar from rotted root and hypocotylsof the sprouts. And the pathogencity tests using test tubes with 2％ water agar and small containers (30 ${\times}$ 30 ${\times}$ 50 cm, WxLxH) cultivation were shown a similar rot on roots and hypocotyls. The fungal mycelium grew rapidly on the water agar and it prevented the seed germination. Density of the Pythium sp. in the recycled water system at the factory was periodically measured using a selective medium, corn meal agar with Pimaricin 10 mg, Rifampicin 10 mg, Ampicillin 100 mg per 1 liter in order to check the contamination of recycled water. After fitering step using 5 and 1 ml in the recycled system was applied and it was effectively controlled Pythium rot. The daily yield of sprout was stable and the occurrenceof Pythium in the recycled water was much less after filtering. The fungal isolates were identified as Pythium deliense Meurs based on various mycological characteristics on corn meal agar and sucrose-asparagine bentgrass leaf culture medium. P. deliens oogonia were spherical, smooth, 19-23 urn in diameter, and their stalk bending toward antheridia. Antheridia were straw hat-shaped, curred club-shaped, therminal or intercalary, monoclinous, occasionally diclinous, 12∼15 ${\times}$ 8∼11 um, 1(∼2) per oogonium.

• 한국미생물·생명공학회지
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• 제21권1호
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• pp.36-40
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• 1993

토양에서 inulin으로부터 di-D-fructofuranose dianhydride(DFA)를 생산하는 inulin fructotransferase를 생산하는 균주로 Enterobacter sp. S45를 선발, 분리하였다. 본 효소에 의하여 생산되는 DFA는 $^13C-nmr$과 HPLC로 분석한 결과 fructose 두 분자가 $\beta$ 1,2': $\alpha$ 2,3' 결합을 한 DFA III로 동정되었다. 본 균주는 탄소원으로 inulin, 유기질소원으로 corn step liquor, 무기질소원으로 $NH_4H_2P0_4$를 사용할 때 효소생산이 가장 많았으며 이 조건하에서 72시간 배양으로 최대 효소생산을 보였다. 균 배양중 배양액내에 DFA III가 생성되었다가 소멸되는 것으로 보아 균체내에 DFAIII를 분해, 이용하는 효소가 존재한다고 추정되었다.

• 한국미생물·생명공학회지
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• 제23권1호
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• pp.6-11
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• 1995

In order to screen microorganism producing lactic acid with high productivity from nature, we used a medium containing 100 g/l glucose and selected several microorganisms producing more than 80 g/l L-lactic acid. We investigated their physiological characteristics and compared them. The best microorganism was identified as Lactobacillus casei subsp. rhamnosus. The optimum pH for growth and production of lactic acid was 6.0 and this strain showed the highest growth rate at around 30$\circ$C , but the optimum temperature for lactic acid production was 45$\circ$C . The growth was inhibited proportionally from 50 g/l to 300 g/l of glucose and the maximal cell mass increased according to increasing the concentration of corn steep liquor (CSL) protein up to 30 g/l. In batch fermentation for lactic acid production, we produced 128 g/l L-lactic acid with 20 g/l CSL protein and 150 g/l glucose in 35 hours. In pH-stat fed-batch fermentation, we were able to produce 183 g/l L-lactic acid.

• 한국초지조사료학회지
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• 제10권3호
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• pp.179-182
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• 1990

본 시험은 고랭지(해발 800m)에서 매초용 옥수수의 추비시기가 생육 및 건물생산에 미치는 영향을 구명코자 수원19호와 강원재래백을 공시하여 198년年 5월 16일에 국럽종축원 대관령지원 시험포장에서 시험을 실시힌바 그 결과를 요약하면 다음과 같다 추비시용 시기의 조만에 따라 작물생육특성인 초장, 웅수의 출현기, 경의 직경, 착수고등의 변화는 거의 없였으며 lOa당 건물수량 및 식물체부 위별 건물수량비솔에 있어서도 처리간에 큰 차이가 인정되지 않았다. 품종간에 있어서 강원재래백이 건물수량 및 경엽수량비솔이 수원19호보다 많은데 대하여 이삭의 비율은 수원19호가 더 높은 것으로 나타났다. 이상의 시험결과로 보아 해발 800m의 고랭지에서 매초용 옥수수를 재배할 경우 추비시용시기간 차이는 크지 않은 것으로 사료된다.

• 한국의류학회지
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• 제32권4호
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• pp.598-607
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• 2008

Bast fibers were applied for various usages from fabrics to household care products long time ago. In this study, we investigated the physical characteristrus of water retted & chemically rotted fibers of Yucca, New Zealand hemp, Corn, Kuzu vine, Indian mallow, and Mulberry paper that have been harvested by domestic cultivation. Water retting is more effective than chemical rotting for six kinds of plant fibers. When all fibers were rotted chemically with 1% sodium hydroxide, only Kuzu vine and Indian mallow were retted. Indian mallow, Yucca, New Zealand hemp, and Com fibers have higher tensile strength than any other fibers. The crystallinity of Kuzu vine, Indian mallow, Yucca, New Zealand hemp, and Corn was as low as 60% but Yucca, New Zealand hemp were flexible. Yucca had fewer lumina whereas New Zealand hemp more lumina in cross sectional shape. Especially com fibers have a structure like sponge, and Indian mallow had a net shape. The longitudinal section of New Zealand hemp showed smooth and long shape. Mulberry paper was proved to be short and thin, which is quite appropriate for making paper. In this study, we found that plant fibers for living material could be used for cloth materials.

• 한국유기농업학회지
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• 제11권1호
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• pp.79-88
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• 2003

본 연구는 찰옥수수의 무경운 재배의 가능성을 구명하고자 5개의 찰옥수수 교잡종을 공시하여 경운 재배와 무경운재배에서 풋이삭수량, 종실구량, 및 주요형질의 반응을 조사하였으며 그 결과는 다음과 같다. 1. 경운 구에 비하여 무경운구에서는 출현율이 4％ 정도 낮았고, 출웅일수와 출사일수가 다소 빨랐으며, 생육초기와 후기의 초장은 길었다. 2. 이삭길이 및 수량구성요소는 경운구와 무경운구의 차이가 없었으나, 교잡종간에는 유의성이 있었다. 3. 무경운구의 풋이삭수량과 종실수량은 경운구와 차이를 보이지 않아 무경운재배의 가능성을 나타내었다. 4. 출웅일수, 출사일수, 초장, 이삭길이는 풋이삭수량 및 종실수량과 상관관계를 나타내었다.

• 한국식품과학회지
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• 제17권4호
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• pp.258-264
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• 1985

부채전분원에서 분리, 동정한 Aspergillus niger를 사용하여 koji를 조제하고 생전분 당화와 에탄을 발호의 조건을 검토한 결과는 다음과 같다. (1) Koji(효소제)는 4일간 배양한 경우에 가장 높은 활성을 가졌고 생전분 당화의 최적pH는 3.3. 최적온도는 $40{\sim}50^{\circ}C$ 이었다. (2) 전분별에 따른 당화는 옥수수전분. 고구마전분. 감자전분의 순위였다. (3) 알코올 발효시 전분의 최초 담금농도는 $20{\sim}30%$이었다. (4) 전분원료중 쌀, 고구마, 옥수수에서 무증자전분의 알코올 발효가 가능함을 보였으나, 증자한 전분들 보다 발효종료일은 약 $1{\sim}2$일 지체되었다. (5) 무증자에 의한 전분의 당파와 동시 알코올 발효에서 $30^{\circ}C$. pH3.3, 100rpm 왕복진탕 발효시 최적발효일은 5일, 쌀, 고구마, 옥수수전분에서 최종 에탄올수율은 약95%이었다.