• Title/Summary/Keyword: cooling time

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$In$ $vitro$ development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification

  • Shin, Mi-Ra;Choi, Hye-Won;Kim, Myo-Kyung;Lee, Sun-Hee;Lee, Hyoung-Song;Lim, Chun-Kyu
    • Clinical and Experimental Reproductive Medicine
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    • v.38 no.4
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    • pp.203-209
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    • 2011
  • Objective: This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. Methods: Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divided into three groups. One group was maintained as fresh controls (n=42), one was frozen by slow freezing (n=43), and one was cooled by vitrification (n=43). After thawing or cooling, survival rates, development to blastocyst, and cell numbers and inner cell mass (ICM) cell numbers of blastocysts were compared with those of the control group. The expressions of eight genes ($Rbm3$, $Birc5$, $Sod1$, $Sod2$, $Cirbp$, $Caspase3$, $Trp53$, $Hsp70.1$) were examined by real time-quantitative polymerase chain reaction in the fresh and frozen-thawed embryos. Results: There were no significant differences in the slow freezing and vitrification groups' survival rate after thawing (88.4% vs. 88.4%), development to blastocyst (100% vs. 97.4%), cell numbers ($107.0{\pm}21.0$ vs. $115.0{\pm}19.7$), or ICM cell numbers of blastocysts ($11.3{\pm}5.2$ vs. $11.1{\pm}3.7$). Cell numbers of blastocysts were significantly ($p$ <0.05) lower in the frozen-thawed embryos than the fresh embryos. There were no significant differences in the slow freezing and the vitrification groups' expressions of the eight genes. The expressions of $CirbP$ and $Hsp70.1$ were higher in the frozen-thawed embryos than in the fresh embryos but there were no significant differences. Conclusion: These results suggest that there were no significant differences between embryos that underwent slow freezing and vitrification.

Analytical and Numerical Model Study to Predict the Temperature Distribution Around an Underground Food Cold Storage Pilot Cavern (냉동저장 공동 주변의 온도분포 예측을 위한 해석해 및 수치모델 적용에 관한 연구)

  • 이대혁;김호영
    • Tunnel and Underground Space
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    • v.12 no.3
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    • pp.142-151
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    • 2002
  • Claesson(2001)'s analytical solution, and two numerical models with Dirichlet and Neuman interior boundary condition respectively were investigated to estimate the transient temperature distribution with distances from the Taejon underground food cold storage pilot cavern. Claesson's solution, which is based on constant temperature boundary condition at the rock wall during a temperature decline step, showed relatively good agreement with temperature measurements in the rock mass in order of average error difference, 0.89$\^{C}$ without any adjustments on laboratory thermal properties to represent the rock mass. For the numerical model with heat flux through the rock wall, a boundary condition setting technique was newly proposed to overcome the difficulty of prescribing variable convective heat tranfer coefficient and far-field air temperature inside the cavern as they may be certainly changed according to the cooling-down time. The results showed also good agreement with measurements in order of average error difference, 1.58$\^{C}$, and were compared to those of the numerical model with fixed temperature at the rock wall. Finally, the most proper procedure to precisely predict the temperature profile around a cavern was proposed as a series of analysis steps including an analytical exact solution and numerical models.

Composition Changes in Cement Matrix of RC Column Exposed to Fire (화재에 노출된 RC기둥 시멘트 매트릭스의 구성성분 변화)

  • Kim, Jung-Joong;Youm, Kwang-Soo
    • Journal of the Korea Concrete Institute
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    • v.26 no.3
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    • pp.369-375
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    • 2014
  • This study examined the changes of microstructural compositions in cement matrix according to the depth from the surface of a reinforced concrete (RC) column exposed to fire. The RC column was exposed to a standard fire for 180 minutes. After the fire test, core samples passing through the column section were obtained. Using the core samples, the remaining fractions of calcium-silicate-hydrates (C-S-H) and calcium hydroxide in cement matrix at the surface, the depth of 40 mm and 80 mm and the center (175 mm) were examined using thermal gravimetric analysis (TGA) and X-ray diffraction analysis (XRDA). Using nuclear magnetic resonance (NMR) technique, the silicate polymerization of C-S-H in cement matrix was also evaluated. The experimental results indicated that the amount of C-S-H loss at the center of column experiencing the transferred fire temperature of $236^{\circ}C$ has been underestimated as the TGA results showed the highest C-S-H contents are located at the depth of 80 mm, where the transferred fire temperature is $419^{\circ}C$. Moreover, the destruction of silicate connections at the center was observed as similar as that at the depth of 40 mm, where the transferred fire temperature was $618^{\circ}C$. This might be attributed to the temperature changes during cooling time after the fire test was neglected. Due to the relatively low thermal conductivity of concrete, the high temperature, which can affect the change of microstructure in cements, will hold longer at the center of the column than other depth.

A CFD Modeling of Heat Generation and Charge-Discharge Behavior of a Li-ion Secondary Battery (Li-ion 이차전지의 충방전 시 발열 및 충방전 특성의 CFD 모델링)

  • Kang, Hyeji;Park, Hongbeom;Han, Kyoungho;Yoon, Do Young
    • Journal of the Korean Electrochemical Society
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    • v.19 no.3
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    • pp.114-121
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    • 2016
  • This study investigates a CFD modeling of the charge-discharge behavior due to heat generation during charge-discharge cycles of a Li-ion secondary battery(LIB). Present LIB system adopted a current-density equation, heat and mass transfer governing equations upon the 1-dimensional system to the thickness direction for the rectangular pouch configuration. According to the 3-kinds of the charge-discharge current densities of 1C($17.5A/m^2$), 3C($52.5A/m^2$) and 5C($87.5A/m^2$) subject to a 3 V of cut-off voltage, a constant-temperature system at 298 K and three different heat generating systems were analyzed with comparison. Battery capacity decreases with increment of charge-discharge densities not only at the constant-temperature system but also at the heat-generating system. The time for charge-discharge cycles increases at the heat-generating system compare to the constant-temperature system. These trends are considered that the increase of temperature due to heat generation causes the decrement of equilibrium potential of electrodes and the increment of diffusivity of Li ions. Furthermore, cooling effects were discussed in order to control the influence of heat generation due to charge-discharge behavior of a Li-ion secondary battery.

The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture (살균조건 및 UV-C 조사가 느타리버섯 병재배 오염율 감소에 미치는 영향)

  • Baek, Il-Sun;Chi, Jeong-Hyun;Jeoung, Yun-Kyeoung;Kim, Jeong-Han;Lim, Jae-Wook
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.256-261
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    • 2015
  • This study was conducted to reduce contamination ratio of oyster mushroom bottle cultivation. The optimal conditions of substrate sterilization for reducing of contamination ratio were at $121^{\circ}C$ for 90 min. In addition, UV-C irradiation is good for lower contamination ratio to continue over 6 hours at cooling and inoculation room after sterilization. The contamination ratio and density of microorganisms of substrate were showed 0% after sterilization at $121^{\circ}C$ for 90 min. Trichoderma sp., main pathogen of mushrooms, was detected from substrate after sterilized during 2 or 4 hours at $101^{\circ}C$ and $105^{\circ}C$, respectively. The amount of electricity used was the lowest at $121^{\circ}C$ for 90 min than that of other sterilization conditions. The UV-C irradiation treatment was used UV-C lamp(40 watts) in the inoculation room($56m^3$). The density of bacteria did not detected after UV-C irradiation for 6 hours. And the death ratio of bacteria and Trichoderma sp. was 99.9% after UV-C irradiation for 6 hours. However, in the same UV-C irradiation time, the death ration of Cladosporium sp. was 90.9%. Therefore, the death ratio of fungi was lower than that of bacteria at the same UV-C irradiation treatment.

Detection of Thermal Ratcheting Deformation for Cylindrical Shells by Ultrasonic Guided Wave (유도초음파를 이용한 원통형 쉘의 열 라체팅 변형 탐지)

  • Joo, Young-Sang;Lee, Hyeong-Yeon;Kim, Jong-Bum;Park, Chang-Gyu;Lee, Jae-Han
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.5
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    • pp.297-305
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    • 2006
  • The thermal ratcheting deformation at the reactor baffle and upper internal structure of the liquid metal reactor (LMR) can occur due to movement of the hot sodium free surface. In in-service inspection of reactor internals of LMR, a new inspection technique should be developed for the detection of the thermal ratcheting damage. In this study, an inspection technique using ultrasonic guided wave is proposed for the detection of the thermal ratcheting damage of cylindrical vessels. A 316L stainless steel cylindrical shell specimen has been prepared. The thermal ratchet structural tests were cyclically performed by heat-up up to $550^{\circ}C$ with steep temperature gradients along the axial direction after cool-down by cooling water. Ultrasonic guided wave propagation has been characterized by analysis of dispersion curve of the stainless steel plate. The zero-order antisymmetric $A_0$ guided wave has been selected as the optimal mode for detection of the ratcheting deformation. It is confirmed that the thermal ratcheting deformation can be detected by the measurement of transit time difference of circumferentially propagated $A_0$ guided waves.

Three-dimensional Numerical Prediction on the Evolution of Nocturnal Thermal High (Tropical Night) in a Basin

  • Choi, Hyo;Kim, Jeong-Woo
    • International Union of Geodesy and Geophysics Korean Journal of Geophysical Research
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    • v.25 no.1
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    • pp.57-81
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    • 1997
  • Numerical prediction of nocturnal thermal high in summer of the 1995 near Taegu city located in a basin has been carried out by a non-hydrostatic numerical model over complex terrain through one-way double nesting technique in the Z following coordinate system. Under the prevailing westerly winds, vertical turbulent fluxes of momentum and heat over mountains for daytime hours are quite strong with a large magnitude of more than $120W/\textrm{m}^2$, but a small one of $5W/\textrm{m}^2$ at the surface of the basin. Convective boundary layer (CBL) is developed with a thickness of about 600m over the ground in the lee side of Mt. Hyungje, and extends to the edge of inland at the interface of land sea in the east. Sensible heat flux near the surface of the top of the mountain is $50W/\textrm{m}^2$, but its flux in the basin is almost zero. Convergence of sensible heat flux occurs from the ground surface toward the atmosphere in the lower layer, causing the layer over the mountain to be warmed up, but no convergance of the flux over the basin results from the significant mixing of air within the CBL. As horizontal transport of sensible heat flux from the top of the mountain toward over the basin results in the continuous accumulation of heat with time, enhancing air temperature at the surface of the basin, especially Taegu city to be higher than $39.3^{\circ}C$. Since latent heat fluxes are $270W/\textrm{m}^2$ near the top of the mountain and $300W/\textrm{m}^2$ along the slope of the mountain and the basin, evaporation of water vapor from the surface of the basin is much higher than one from the mountain and then, horizontal transport of latent heat flux is from the basin toward the mountain, showing relative humidity of 65 to 75% over the mountain to be much greater than 50% to 55% in the basin. At night, sensible heat fluxes have negative values of $-120W/\textrm{m}^2$ along the slope near the top of the mountain and $-50W/\textrm{m}^2$ at the surface of the basin, which indicate gain of heat from the lower atmosphere. Nighttime radiative cooling produces a shallow nocturnal surface inversion layer with a thickness of about 100m, which is much lower than common surface inversion layer, and lifts extremely heated air masses for daytime hours, namely, a warm pool of $34^{\circ}C$ to be isolated over the ground surface in the basin. As heat transfer from the warm pool in the lower atmosphere toward the ground of the basin occurs, the air near the surface of the basin does not much cool down, resulting in the persistence of high temperature at night, called nocturnal thermal high or tropical night. High relative humidity of 75% is found at the surface of the basin under the moderate wind, while slightly low relative humidity of 60% is along the eastern slope of the high mountain, due to adiabatic heating by the srong downslope wind. Air temperature near the surface of the basin with high moisture in the evening does not get lower than that during the day and the high temperature produces nocturnal warming situation.

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Change of Skin Temperature of Workers Using Vibrating Tools in Anthracite Mines (진동공구 사용근로자의 피부온도 변화)

  • Roh, Jae-Hoon;Moon, Young-Hahn;Shin, Dong-Chun;Cha, Bong-uk;Cho, Soo-Nam
    • Journal of Preventive Medicine and Public Health
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    • v.21 no.2 s.24
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    • pp.357-364
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    • 1988
  • By implementing epoch-making policies for industrial promotion, the national economy has made a remarkable development. As a result of such economic growth, industrial accidents and occupational diseases have become a serious problem in Korean society. In the presidential order for the execution of the Korean Labor Standard Law, neuritis and other diseases stemming from health impairments due to vibrations in industrial processes are designated to be dealt with as vibration diseases. In the case of vibration disease, industrial accident compensation is not effectively paid. In order to investigate the vibration hazards of rock-drill operation, the authors studied the subjective symptoms and performed physical function tests on a total of 79 persons (vibration exposed group) who used rock-drills, and 39 persons (control group) who did not use rock-drills at anthracite mines. The results of the physical function test were as follosws : 1. The right hand was more affected by white finger than the left hand. 2. Independent variables such as duration of rock-drill operation, age, drinking and smoking were indentified as statistically significant factors for the occurrence of white finger. 3. In the pain sense threshold, the group with Raynaud's phenomenon showed a statistically higher level than that of the control group. 4. The skin temperature of the group with Raynaud's phenomenon was lower than that of the control group. The recovery time of skin temperature aftr cooling was delayed compared with the value of the control group.

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Crystal growth of phospho-gypsum in Na2SO4 solution (황산나트륨 용액에서 인산부생석고의 결정성장 특성)

  • Lee, Gye-Seung;Lee, Jung-Mi;Song, Young-Jun;Shin, Kang-Ho;Kim, Youn-Che;Yoon, Si-Nae;Jang, Yoon-Ho;Lee, Sung-Riong
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.21 no.1
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    • pp.6-14
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    • 2011
  • The re-crystallization process for recovering granular crystalline gypsum from phospho-gypsum have proposed. The process consists of two stages; at the first stage, needle-like fine gypsum is recovered with under-product of 325 mesh wet screening after the speedy hydration of dehydrated phospho-gypsum, and at the second stage, granular crystalline gypsum is recovered with upper-product of 325 mesh wet screening after dehydration and re-crystallization of the needle-like fine gypsum in $Na_2SO_4$ solution. This paper is mainly focused on the influence of dehydration time, cooling speed of temperature and re-crystallization temperature on the recovery of granular crystalline gypsum. And the re-crystallization velocity of needle-like fine gypsum at room temperature and the variation of $Ca^{2+}$ concentration of gypsum slurry during over all re-crystallization process were also discussed.

A Study on Electrodeionization for Purification of Primary Coolant of a Nuclear Power Plant (원자력 발전소의 일차 냉각수 정화를 위한 전기탈이온법의 기초연구)

  • Yeon, Kyeong-Ho;Moon, Seung-Hyeon;Jeong, Cheorl-Young;Seo, One-Sun;Chong, Sung-Tai
    • Journal of Radiation Protection and Research
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    • v.24 no.2
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    • pp.73-86
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    • 1999
  • The ion-exchange method for the purification of primary coolant has been used broadly in PWR(pressurized water reactor)-type nuclear power plants due to its high decontamination efficiency, simple system, and easy operation. However, its non-selective removal of metal and non-radionuclides shortens its life, resulting in the generation of a large amount of waste ion-exchange resin. In this study, the feasibility of electrodeionization (EDI) was investigated for the purification of primary cooling water using synthetic solutions under various experimental conditions as an alternative method for the ion exchange. The results shows that as the feed flow-rate increased, the removal efficiency increased and the power consumption decreased. The removal rate was observed as a 1000 decontamination factor(DF) at a nearly constant level. For the synthetic solution of 3 ppm TDS (Total Dissolved Solid), the power consumption was 40.3 mWh/L at 2.0 L/min of feed flow rate. The higher removal rate of metal species and lower power consumption were obtained with greater resin volume per diluting compartment. However, the flow rate of the EDI process decreased with the elapsed time because of the hydrodynamic resistivity of resin itself and resin fouling by suspended solids. Thus, the ion-exchange resin was replaced by an ion-conducting spacer in order to overcome the drawback. The system equipped with the ion-conducting spacer resolved the problem of the decreasing flow rate but showed a lower efficiency in terms of the power consumption, the removal rate of metal species and current efficiency. In the repeated batch operation, it was found that the removal efficiency of metal species was stably maintained at DF 1000.

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