• Title/Summary/Keyword: controlled culture

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A Study on Implementation of Hydroponics Automation System using Adaptive Fuzzy Control (적응 퍼지 제어기를 이용한 수경재배 자동화를 위한 연구)

  • 노명균;김승우;홍상은
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 1996.10a
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    • pp.111-114
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    • 1996
  • Hydroponics is to grow plants, not in soil but in water which the quantity of necessary chemical food can be controlled. In this paper, this is designed in the automatic system. The closed culture reduces cost of production and produces a many kinds of agricultural products in a confined place. An adaptive fuzzy control in the best method to solve and to overcome parametric uncertainties and non-linearity of the controlled system. A hydroponics automation system which is able to overcome these control problems. It is used in implementation of the hydroponics automation system. The performance is analyzed through an experiment in which the new adaptive fuzzy control method is applied to the automatic control of tomato hydroponics.

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A Critical Review on the use of Media Platform in Dance: Focused on popularization strategy (무용의 미디어 플랫폼 활용에 대한 비판적 고찰: 대중화 전략을 중심으로)

  • YIM, Sujin
    • Trans-
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    • v.5
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    • pp.29-48
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    • 2018
  • This study examines the popularization strategy of domestic dance field through media platform and examines it based on the interaction of cultural participants. What does popularization of dance ultimately mean? What are the characteristics of media-based popularization strategies? Can all of these attempts be understood as popularization strategy with same attributes? The purpose of this study is to examine the characteristics of broadcasting media, which are used as the main medium of dance popularization strategy, and the online platform that recently attracts attention, and to focus on the communication process of cultural participants related to each media. Through this approach, media based activities turns out to be Mass Culture, controlled by businessman, capital, making it impossible for the creator to communicate directly with the audience, and producing the cultural products planned by the businessman. On the other hand, an online community platform is classified as Popular Culture since it allows creator to communicate and interact with audience without the direct involvement of the businessman and guarantees the rights of creation. Thereby it function as appropriate media platform and expand the discourse on popularization of dance in Korea.

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Operational Strategy for Increasing Ethanol Production in Repeated Fed-batch Ethanol Fermentation Using Saccharomyces cerevisiae (Saccharomyces cerevisiae 를 이용한 반복 유가식 ethanol 발효에서 ethanol 생산량을 증가를 위한 운전 전략)

  • Lee, Sang-Eun;Seo, Hyeon-Beom;Kwon, Min-Cheol;Lee, Hyeon-Yong;Jung, Kyung-Hwan
    • KSBB Journal
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    • v.25 no.2
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    • pp.187-192
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    • 2010
  • We designed the optimal operational strategy in repeated fed-batch ethanol fermentation using Sacchromyces cerevisiae ATCC 24858 in views of ethanol yield, specific ethanol production rate, and ethanol productivity, when the aeration rate were controlled at 0.0 and 0.33 vvm. Coincidentally, the time intervals of withdrawal-fill of culture medium (24 and 36 h) were investigated. Ethanol yield and ethanol productivity when the aeration was carried out at 0.33 vvm were superior to those when the aeration was not carried out. Additionally, those parameters when the time interval of withdrawal-fill of culture medium was 24 h were superior to those when time interval of withdrawal-fill of culture medium was 36 h. The total ethanol production reached at the greatest value, 703.8 g-ethanol, when the aeration was carried out at 0.33 vvm and the time interval of withdrawal-fill of culture medium was 24 h. In this study, we verified experimentally the necessity of designing the operational strategy for increasing ethanol production in terms of aeration rate and time interval of withdrawal-fill of culture medium in the repeated fed-batch ethanol fermentation.

Morphological Changes by Submerged Culture Conditions for the Mycelial Optimal Growth of Cordyceps sinensis and Immunological Properties of Hot Water Extract of Mycelium (동충하초 균사체 최적 성장을 위한 심부배양 조건에 따른 형태학적 변화 및 균사체 열수 추출물의 면역학적 특성)

  • Suh, Hyung-Joo;Choi, Jang-Won
    • KSBB Journal
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    • v.25 no.1
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    • pp.47-54
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    • 2010
  • The mycelial dispersed growth of Cordyceps sinensis was optimized in submerged batch culture at initial pH of 5.0, 150 rpm, and $25^{\circ}C$. The morphological data showed much more dispersed growth of C. sinenesis at initial pH of 5.0. Also, projected area, main hyphal length and number of tips for the mycelial growth of initial pH 5.0 were higher than those of other initial pHs. The industrial medium for mycelial production of C. sinensis was determined to be molasses of 100 g and crushed brewery yeast of 10 g per liter as carbon and nitrogen sources, respectively. With these culture conditions, the maximum production of mycelia was approximately 30.0 g per liter by batch culture in 5-liter jar fermenter with no controlled pH. This result suggests that large-scale mycelia production of C. sinensis may be possible in submerged batch culture. The hot water extract of mycelia from C. sinensis was mainly composed of 83.0% carbohydrate, 11.8% protein, 1.9% lipid, and 2.4% ash and there were present glucose, mannose, galactose, and arabinose as molar ratio of 8.79 : 2.59 : 1.34 : 1.0 in the carbohydrate, respectively. In the experiment using spleen cell and macrophage, the extract showed potent mitogenic and immuno-stimulating activities and among various components, an important factor that contribute to the immunological activities was turned out to be carbohydrate moiety.

Effect of Different pH Processes on Branched β-1,3-Glucan Production from Submerged Culture of Ganoderma lucidum (영지 (G. lucidum)의 액체배양에 의한 β-1,3-Glucan 생산에 미치는 서로 다른 pH Process의 영향)

  • Lee, Shin-Yaung;Lee, Kyu-Min
    • Journal of Industrial Technology
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    • v.20 no.A
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    • pp.45-50
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    • 2000
  • A submerged cultivation of Ganoderma lucidum was carried out in an air-lift fermenter system, and the effects of different pH processes on extracellular branched ${\beta}$-1,3-glucan(EPS) production and mycelial growth(MDW) were investigated. The controlled pH process improved the production of branched ${\beta}$-1,3-glucan and biomass in comparison to the uncontrolled pH process. However, the maximum production of branched ${\beta}$-1,3-glucan were obtained by the bi-staged pH process. From these results, we confirmed that the bi-staged pH process was the most effective for improving the production of branched ${\beta}$-1,3-glucan from submerged culture of G. lucidum.

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Design of an Intelligent Streetlight System in USN

  • Oh, Sun Jin
    • International Journal of Advanced Culture Technology
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    • v.2 no.2
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    • pp.1-6
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    • 2014
  • In this paper, we propose an intelligent streetlight system that has a complex sensor module of temperature, humidity, luminance and motion detection and controlled by the fuzzy logic based central monitoring system in order to get flexible and precise manipulation of the streetlight system in USN environment. The proposed streetlight system provides low power consumption and high efficiency by using sensed data from the complex sensor module, which were collected, processed, and analyzed by the fuzzy logic based central monitoring system. The performance of the proposed streetlight system is to be evaluated by a simulation study in terms of power savings and safety at the fields constructed as a test-bed under several suggested scenarios. Finally, we know that the proposed intelligent streetlight system can maximize the energy savings efficiently with the fuzzy logic based central monitoring system and selective remote dimming control by connecting it to the wireless ubiquitous sensor network (USN) using a Zigbee module.

Production of Nuclease Activity in U937 Cells by Phorbol 12-Myristate 13-Acetate and Lipopolysaccharide

  • Kwon, Hyung-Joo;Kim, Doo-Sik
    • BMB Reports
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    • v.36 no.5
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    • pp.520-523
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    • 2003
  • The proliferation and differentiation signals of myelogeneous U937 cells are provided by extracellular stimuli, such as lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA). In a DNA-native-polyacrylamide gel assay system, we demonstrated that a particular nuclease activity is expressed in PMA-stimulated U937 cells and secreted into the culture medium. The nuclease activity was induced in U937 cells by LPS treatment, while the secretion of the enzyme was undetected in the culture medium. Therefore, it is likely that the expression and secretion of the particular nuclease in U937 cells are controlled by extracellular stimulations, such as PMA and LPS treatment.

Effect of a Microbial Product on the Control of Soilborne Diseases of Turfgrasses (미생물제에 의한 잔디의 토양전염병 방제 효과)

  • 박규진;김영호;박은경;김동성
    • Plant Disease and Agriculture
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    • v.1 no.1
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    • pp.19-29
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    • 1995
  • A microbial product composed of three antagonistic fungal isolates (Aspergillus sp., Penicillium sp. and Trichoderma sp.) and three bacterial isolates (Arthrobacter sp., Bacillus sp., and Pseudomonas sp.) was tested for the control of Pythium blight caused by Pythium sp., brown patch by Rhizoctonia solani (anastomosis group(AG) 1-1) and large patch by R. solani (AG 2-2) of turfgrasses. Cultures of the antagonistic fungi and bacteria varied in the effectiveness in reducing disease severity of Pytium blight and brown patch on bentgrass. The antagonistic fungal and bacterial isolates were mixed and cultured at 20-$25^{\circ}C$ for 3 days in a growth medium, and the diluted solution of the microbial culture was applied under the field conditions after inoculation of the above turfgrass pathogens. The treated turfgrass was incubated at 28$^{\circ}C$ in a growth chamber. In this experiment, Pythium blight was almost completely controlled and brown patch was slightly decreased by the microbial product, while no control was observed in large patch of zoysiagrass. In zoysiagrass treated with the microbial culture, thatch accumulation was notably reduced.

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Biocontrol Effect of Gliocladium virens G1 and Soil Amendment on Astragal Stem Rot Caused by Rhizoctonia solani

  • Chung, Bong-Koo;Yun, Kyung-Ho
    • Mycobiology
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    • v.28 no.4
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    • pp.180-184
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    • 2000
  • In order to find an environment-friendly method to suppress astragal stem rot caused by the isolates of Rhizoctonia solani AG 1 and AG 4, we tested an antagonistic fungus Gliocladium virens G1 was evaluated as a biocontrol agent and estimated inorganic compounds and organic materials were tested for their effect of the disease suppression. G. virens G1 effectively inhibited mycelial growth in a dual culture and caused mycelial lysis in the culture filtrate. No adverse effect was observed when examined for seed germination and seedling growth. Promoted seedling growth was observed with the seed treatment. Seeds of astragal plant were germinated higher in the sterile soil than the natural soil. Of 14 inorganics tested, alum, aluminum sulfate and calcium oxide significantly suppressed the mycelial growth and sclerotial germination. Milled pine bark and oak sawdust also suppressed the mycelial growth. Soil amended with 1% of G. virens G1 composted with pine bark (w/v) significantly controlled astragal stem rot in the glasshouse experiments.

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Corynebacterium sp. K-199가 생산하는 단백질성 생물응집제

  • Kim, Young-Jun;Choi, Yang-Mun;Cho, Hong-Yon;Yang, Han-Chul
    • Microbiology and Biotechnology Letters
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    • v.24 no.6
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    • pp.699-704
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    • 1996
  • About 600 microorganisms isolated from soil, marsh, compost, etc. were examined for their flocculating ability in the kaolin suspension and swine wastewater. Among them, K-199 was the best producer of flocculant and was identified to be a species belong to the genus Corynebacterium. The maximum production of the flocculant from Corynebacterium sp. K-199 was observed in culture medium containing 2% glycerol, 0.4% peptone, 0.3% K$_{2}$HPO$_{4}$, 0.1% CaCO$_{3}$, 0.05% NaCl, 0.1% yeast extract, 0.1% MgSO$_{4}$,7H$_{2}$O and initial pH 7.5 when cultured with rotary shaker controlled at 25$\circ$C and 150 rpm. Under the optimal culture condition with jar fermentor, the maximum production was reached to flocculating activity of 780 units/ml after 4 days. From the results of the activity be fully maintained by periodate oxidization, it suggests that the activity is due to the protein.

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