• Journal of Bio-Environment Control
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• v.2 no.1
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• pp.9-15
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• 1993

Chinese white cabbage, Chinese flat cabbage, lettuce, garland chrysanthemum, and green perilla were grown in nutrient solution culture to investigate the effects of various media and nutrient solutions. The culture media were sand, mixed substrate(peatmoss : sand= 1 : 1), and non-media(deep-flow culture). The nutrient solutions were Cooper's, Hoagland's, and Yamazaki's solution. Plants were grown under different treatments for three weeks. Generally, the growth was greatest in non-media culture and followed mixed substrate culture, and poorest in sand culture. In non-media culture, the growth of Chinese white cabbage, Chinese flat cabbage, lettuce, and green perilla was good in Yamazaki's solution. And regardless of nutrient solution, garland chrysanthemum was good in non-media culture. Relative chlorophyll was not different among the treatments.

• KSBB Journal
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• v.18 no.2
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• pp.145-148
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• 2003

The hydrogen-producting bacterium was isolated from fresh water and identified as Enterbacter cloacae. The isolated was named Enterobacter cloacae YJ-1. In batch culture, The optimum cultivation temperature and pH of strain YJ-1 was 35$^{\circ}C$ and 7.5, respectively. All of the added glucose was consumed completely during fermentation even though pH was not controlled. Amount of hydrogen produced on each condition of 2% glucose, 4% sucrose and 5% fructose was 950, 1000 and 948 mL/L, respectively and resulted in increasing hydrogen production approximately 2.5-times more than controlled condition. The macimum hydrogen production was obtained when 50mM phosphate was added. was obtained when 50mM phosphate was added. In repeated0batch culture, yeast extract, but the production amount was not changed on the condition of over 0.5%, Most of the organic acides produced during the fermentation were formic and acetic acid, and propionic acid was moiety also generated.

• KSBB Journal
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• v.16 no.4
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• pp.415-419
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• 2001

To produce staphylokinase (SAK) in B. subtilis WB700, media optimization was carried out and the operation of batch and fed-batch fermentation were compared. Tryptone is a good nitrogen source and its optimum concentration in modified super rich(MSR) media is 15 g/L. When glucose is used as a limiting carbon source in the MSR media, 5 g/L of an optimum glucose concentration was identified for the SAK production under the control of P43 promoter. As the expression of P43 promoter is controlled by the limitation of oxygen, the SAK production was controlled at the 30% DO level in the fed-batch fermentation. Unexpectedly, batch fermentation using MSR media showed 1.5 times higher yield of SAK than that of the fed-batch fermentation. The main cause of the results comes from not achieving higher cell concentration in the fed-batch fermentation and the optimum expression level of P43 promoter under oxygen or nutrient limitations. We could not achieve the increase in cell concentration by any means in batch culture as well as fed-batch culture. The highest yield in the batch culture was 2880 units of SAK activity and 455 mg/L of secreted SAK.

• Journal of Digital Convergence
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• v.15 no.4
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• pp.427-441
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• 2017

The purpose of this research is to examine the effect EVE about culture & art education and social tendencies have on adult educatees eliminating controlled mechanisms. Quantitative research on those in their 40s and 50s was executed. The results show that there is a high correlation between GEVE and individualism, REVE and collectivism. GEVE has a higher influence on actualization of self-efficiency, FEVE on reignition of retained desires, REVE on substantialization of non-isolation, individualism on actualization of self-efficiency, and collectivism on substantialization of non-isolation. This research evokes the importance of an interdisciplinary and integrated understanding of EVE in culture & art education, educatees' social tendencies, and their psychological mechanisms. Also, it suggests the necessity of following research and practical adaptation related to developing programs, securing quantitative and qualitative appropriateness reflecting social requirements, and operation, evaluation, and management considering reciprocality.

• The Research Journal of the Costume Culture
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• v.14 no.1
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• pp.153-167
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• 2006

The hip-hop, which began with subculture of black people in Harlem, have changed variously in musical history after the times, which has become the culture phenomenon that had controlled dancing, fashion, and further consciousness of the new generation around the world in 1990s. Since Jean Patou first used Graffiti on clothing in 1920s, graffiti art has been expressed through various methods in many designers' works. The purpose of this study is to develop 'The Hip-Hop' clothing design which express major images in works of Jean-Michel Basquiat, a representative painter of Graffiti art in modern fashion. The concept is Jean Casual Look representing Young Street Culture. Breaking from existing strong legitimate hip-hop styles, clothing design expresses funny, healthy, comfortable, and active feelings. Adobe Illustrator and Adobe Photoshop program were used to design Hip-Hop clothing. With the result that the images on Basquiat's works applied to the hip-hop clothing design, it is suggested that the works of artists are worthy of practical use as factors of a high value-added design, which corresponds with collaboration appearing in modern design variously.

• KSBB Journal
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• v.13 no.2
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• pp.203-208
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• 1998

We investigated the effects of dissolved oxygen (D.O.) and fructose (C-source) on cell growth and biosynthesis of cyclosporin A (CyA) produced as a secondary metabolite by a wild-type filamentous fungus, Tolypocladium inflatum. This was performed by controlling the level of D.O. and the residual C-source, as required, through adjustment of medium flow rate, medium concentration and agitation rate in fed-batch cultures. CyA production was furned out to be maximal, when D.O. level was controlled around 10% saturated D.O. and concentration of the C-source was maintained sufficiently low (below 2 g/L) not to cause carbon catabolite repression. Under this culture condition, we obtained the highest values of CyA concentration (507.14 mg/L), Qp (2.11 mg CyA/L/hr), $Y_x/s$ (0.49 g DCW/g fructose), $Y_p/s$<(22.56 mg CyA/g fructose), and YTEX>$_p/x$ (48.31 mg CyA/g DCW), but relatively lower values of cell concentration (11.98 g DCW/L) and cell productivity (0.043 g DCW/L/hr), in comparison with other parallel fed-batch fermentation conditions. These results implied that, in the carbon-limited culture with 10% saturated D.O. level, the producer microorganism utilized the C-source more efficiently for secondary metabolism.

• Preventive Nutrition and Food Science
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• v.6 no.4
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• pp.206-210
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• 2001

Starch is an abundant resource in plant biomass, and it should be hydrolyzed enzymatically into fermentable sugars for ethanol fermentation. A genetic recombinant yeast, Saccharomyces cerevisiae GA-7458, was constructed by integrating the structural gene of both $\alpha$-amylase from Bacillus stearothermophilus and the gene (STA1) encoding glucoamylase from S. diastaticus into the chromosome of S. cerevisiae SH7458. The recombinant yeast showed active enzymatic activities of $\alpha$-amylase and glucoamylase. The productivity of ethanol fermentation from the pH-controlled batch culture (pH 5.5) was 2.6 times greater than that of the pH-uncontrolled batch culture. Moreover, in a fed-batch culture, more ethanol was produced (13.2 g/L), and the production yield was 0.38 with 2% of corn starch. Importantly, the integrated plasmids were fully maintained during ethanol fermentation.

• Current Research on Agriculture and Life Sciences
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• v.10
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• pp.157-164
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• 1992

The study was conducted to get basic information for haploid production of Petunia hybrida through anther culture by investigating several factors, namely anther stage, culture medium, cold treatment, and genotypes. The results are summarized as follows; Four genotypes of Petunia hybrida were used in a study of anther culture. Plants of each genotype were grown in controlled environments at $20-30^{\circ}C$ with a 16h photoperiod. Equal numbers were harvested from each genotype. The anthers were taken from buds which had received the 14 days' cold treatment at $4^{\circ}C$. Anthers were dissected out aseptically and plated on 1/2 strength MS agar medium containing 5.0mg/${\ell}$ 6-Benzylaminopurine(BAP). Four weeks after culture, light green callus was formed. From these calli, plantlets were regenerated on MS medium containing 2.0mg/${\ell}$ 2-isopentenyl adenine(2ip) after 3 weeks.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.25 no.4
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• pp.203-208
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• 2014

Objectives : Art and culture education might play a good role in children's emotions ; however, few studies to verify this have been conducted. The objective of this study was to examine the effect of an art and culture education program on behavior and social skills in children aged 7-10 years. Methods : Fifteen children (mean age $7.31{\pm}0.95$ years) were recruited from a community center and participated in an art and culture education program. The participants received a once a week program for 17 sessions, and we compared scores on the Child Behavior Checklist (CBCL) and Social Skills Improvement System-Rating Scales (SSIS-RS) before and after intervention. Results : The participants' subscale scores of CBCL-aggressive behavior, social immaturity, internalizing behavior, externalizing behavior, total behavior problems and social skill scores on the SSIS-RS showed improvement (p<.05) after 15-weeks of the art and culture education program. Conclusion : These results suggest a positive effect of an art and culture education program on the reduction of externalizing and internalizing behaviors and improvement of social skills in children. Further prospective, controlled studies in large samples are needed in order to confirm our findings.

• Journal of Biosystems Engineering
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• v.39 no.3
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• pp.244-252
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• 2014

Purpose: The development of an efficient in vitro cell culture device to process various cells would represent a major milestone in biological science and engineering. However, the current conventional macro-scale in vitro cell culture platforms are limited in their capacity for detailed analysis and determination of cellular behavior in complex environments. This paper describes a microfluidic-based culture device that allows accurate control of parameters of physical cues such as pressure. Methods: A microfluidic device, as a model microbioreactor, was designed and fabricated to culture Saccharomyces cerevisiae and Chlamydomonas reinhardtii under various conditions of physical pressure stimulus. This device was compatible with live-cell imaging and allowed quantitative analysis of physical cue-induced behavior in yeast and microalgae. Results: A simple microfluidic-based in vitro cell culture device containing a cell culture channel and an air channel was developed to investigate physical pressure stress-induced behavior in yeasts and microalgae. The shapes of Saccharomyces cerevisiae and Chlamydomonas reinhardtii could be controlled under compressive stress. The lipid production by Chlamydomonas reinhardtii was significantly enhanced by compressive stress in the microfluidic device when compared to cells cultured without compressive stress. Conclusions: This microfluidic-based in vitro cell culture device can be used as a tool for quantitative analysis of cellular behavior under complex physical and chemical conditions.