• Journal of the Korean Society for Precision Engineering
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• v.26 no.3
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• pp.137-143
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• 2009

It has been reported that mechanical stimulation takes a role in improving eel/ growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate cell-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly For dynamic cell-culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell-culture using this agarose gel and developed bioreactor in 3 days.

• Microbiology and Biotechnology Letters
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• v.27 no.1
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• pp.80-85
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• 1999

To develop bioflocculant for wastewater treatment, about 60 type culture strains and 450 strains isolated from natural sources were examined for screening their ability to flocculate the swine wastewater. Among them, YWO-5 showed the highest activity for NTU removal efficiency and was identified as Bacillus megaterium according to the cultural, morphological and physiological properties. The maximum production of the flocculant was achieved in culture medium containing 2% glucose, 0.05% soytone, 0.01% $CaCl_2$, 0.05% $KH_2PO_4$, and 0.05% yeast extract with initial pH 6.5 when cultured with rotary shaker controlled at 20$^{\circ}C$ and 150 rpm. With jar fermentor, the maximum production was reached to NTU removal efficiency of 93% after 3 days under the optimal conditions. The bioflocculant produced by Bacillus megaterium YWO-5 was effective on various suspended solids and organic wastewaters.

• Microbiology and Biotechnology Letters
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• v.27 no.4
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• pp.327-332
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• 1999

A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Breviabcterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1g/L. The optimal feeding rate of L-arginine was 0.028g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L-ornithine concentrations within 64hrs of cultivation in 5 and 50 liter fermenters were 73g/L and 71g/L, respectively. The maximum overall L-ornithine productivity was 1.14g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application.

• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.419-427
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• 1991

Ethanol fermentation of glucose by a strain of Saccharomyces cereuisiae was studied in membrane recycle bioreactor, where the fermentation vessel was coupled with cross flow hollow fiber membrane. The cell recycle system controlled backflushing with fresh medium was proven to be effective in alleviating membrane fouling and allowing long term operation of high-cell continuous fermentation. Using 100 g/l initial glucose concentration, the maximum productivity of about 9 5 g/$l \cdot h$ has been achieved at dilution rate 2.5 $h^{-1}$ and bleed stream ratio 0.05 with the corresponding ethanol concentration of 35g/l and glucose conversion of 100%. Increasing the glucose concentration to 200 g/$l \cdot h$ resulted in an increase in ethanol concentration to 48 g/l and productivity to 120 g1l.h. Substrate conversion, however, was only 69%. This productivity was the highest value in the study, and about 38 fold more than that of batch culture and 17 fold more that of single stage continuous culture without cell recycling. No further increase in the productivity was obtained when the glucose concentration was increased reased to 300g/l.

• The International Journal of Costume Culture
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• v.3 no.1
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• pp.30-40
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• 2000

The purpose of this research was to device thermal comfort model for radiation power plant workers in protective clothing. Three fabrics commonly used in protective workwear were made into coveralls of identical design and were evaluated by adult healthy males in four simulated work environment. It was investigated between the physiological response and subjects comfort according to environmental variance and clothing types. The of simulated work enviro mensent was controlled under four different humidity and temperature of each type. (Temperature 20±1℃, RH 40∼70%±5%, Temperature 30±1℃, RH 40∼70%±5%) An index of physiological response was connected with the thermal comfort designed. Mean skin temperature, skim temperature, Axillasy temperature ear canal temperature, clothing climate, total sweat, blood pressure, and R-R interval were be evaluated. Skin temperature difference ocurring during exercise and rest were significant only with respect to time and regions of the body, This despite physical differences in the three coveralls, particulary mass statistically experiment. Also, an index of subject wearing sensation was designed for thermal comfort after investigation determined the kind of clothes and the type of environment. As a result of this research, two types of multiple regressions was deviced to estimate thermal comfort of the protective clothing.

• International Journal of Advanced Culture Technology
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• v.4 no.2
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• pp.47-56
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• 2016

In transformer cooling systems, oil temperature is controlled through the use of a blower and oil pump. For this paper, set-point algorithms, a reset algorithm and control algorithms of the cooling system were developed by neural networks and fuzzy logics. The oil inlet temperature was set by a $2{\times}2{\times}1$ neural network, and the oil temperature difference was set by a $2{\times}3{\times}1$ neural network. Inputs used for these neural networks were the transformer operating ratio and the air inlet temperature. The inlet set temperature was reset by a fuzzy logic based on the transformer operating ratio and the oil outlet temperature. A blower was used to control the inlet oil temperature while the oil pump was used to control the oil temperature difference by fuzzy logics. In order to analysis the performance of these algorithms, the initial start-up test and the step change test were performed by using the dynamic model of a transformer cooling system. Test results showed that algorithms developed for this study were effective in controlling the oil temperature of a transformer cooling system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.4
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• pp.328-334
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• 2002

A Lindernia dubia (L.) Pennell var. dubia accession from Jeonnam province, Korea was tested for resistance to sulfonylurea (SU) herbicides, imazosulfuron and pyrazosulfuron-ethyl in whole-plant response bioassay. The accession was confirmed resistant to both herbicides. The $GR_{50}$ (herbicide concentration that reduced shoot dry weight by 50%) values of resistant accession were 264 and 19 times higher to imazosulfuron and pyrazosulfuronethyl, respectively, than that of the standard susceptible accession. The surviving resistant L. dubia after pyrazosulfuron-ethyl + molinate application can be controlled by sequential applications of soil-applied herbicides, butachlor, dithiopyr, pyrazolate, and thiobencarb and foliar herbicides, bentazon. Sulfonylurea-based mixtures such as mixtures of azimsulfuron + anilofos, bensulfuron-methyl + oxadiazon, pyrazosulfuron-ethyl + fentrazamide, and pyrazosulfuron-ethyl + anilofos + carfentrazon can also be used to control the surviving resistant L. dubia. However, use of these mixtures should be restricted to a special need basis. Thus, we suggest that sequential applications of non-SU-based mixtures such as butachlor + pyrazolate and MCPB + molinate + simetryne be used to control the surviving resistant L. dubia after SU herbicide applications. Rice yield was reduced 24 % by resistant L. dubia that survived after the pyrazosulfuron-ethyl + molinate application compared with pyrazolate + butachlor in transplanted rice culture. In vitro ALS activity of the resistant biotype was 40 and 30 times more resistant to imazosulfuron and pyrazosulfuron-ethyl, respectively, than the susceptible biotype. Result of in vitro ALS assay that the resistance mechanism of L. dubia to SU herbicides may be due, in part, to an alteration in the target enzyme, ALS.

• Pediatric Infection and Vaccine
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• v.5 no.1
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• pp.136-138
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• 1998

Shigella spp. cause classic bacillary dysentery that rarely result in extraintestinal complications. Urinary tract infections(UTIs) due to Shigella spp. are rare, and Shigella sonnei UTIs are extremely rare. We report a case of symptomatic UTI due to S. sonnei. A 9-year-old female presented with a history of fever, abdominal pain, loose form diarrhea, vomiting, and dysuria for 1 day. S. sonnei was identified from urine culture and stool culture result was no Salmonella and Shigella isolated. She was treated with gentamicin and cefuroxime intravenously for 5 days, which suscessfully controlled clinical features of infections.

• Journal of the Korean Society of Food Culture
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• v.32 no.5
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• pp.394-402
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• 2017

This study evaluated how fresh pear cognition variables influences the purchase of primary and secondary products to promote pear consumption. The survey was performed online using 200 consumers who have consumed pears. Consumers' cognition was classified into two groups, one that was more favorable toward fresh pears and one that was less favorable. The cognition toward pear significantly affected the attitude (p<0.001) and purchase intention (p<0.001) toward fresh pear. When consumers bought fresh pear, sweetness was most important among the selection attributes to both groups, and the most common reason why consumers liked the pear analyzed by check all that apply (CATA) was also sweet taste. However, the negative group did not prefer fresh pear because of taste and texture, so those qualities should be well-controlled. In addition, cognition toward primary products influenced liking and purchase intention of processed pear products, especially beverages (p<0.001), bakeries and sweets (p<0.05). Therefore, these types of secondary products should be developed based on consumers' demands.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.117-122
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• 1992

This experiment was conducted to determine tuber yield increase of Konjac by sowing time, seed tuber split method and cultural methods. Tuber yield per unit area was generally increased in early planting than conventional planting time. Tuber yield of polyethylene film mulching culture among storage methods was high, while the emergence rate of konjac for seedling plus PE mulching, and tunnel culture were prompted by 20 days, and especially seedling was to be greatly controlled due to temperature difference of the day and night at emergence. All of the cutting methods(two and four split method) were higher than conventional method because of increasing number of bulblet, accordingly, that method using seed tuber was profitable for mass propagation. Both botanical characteristics and tuber yield tend to be increased at 30 to 50 percent shading than natural condition.