• Journal of Microbiology
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• 제44권2호
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• pp.155-161
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• 2006

Comparative analysis of microbial communities in a sequencing batch reactor which performed enhanced biological phosphorus removal (EBPR) was carried out using a cultivation-based technique and 16S rRNA gene clone libraries. A standard PCR protocol and a modified PCR protocol with low PCR cycle was applied to the two clone libraries of the 16S rRNA gene sequences obtained from EBPR sludge, respectively, and the resulting 424 clones were analyzed using restriction fragment length polymorphisms (RFLPs) on 16S rRNA gene inserts. Comparison of two clone libraries showed that the modified PCR protocol decreased the incidence of distinct fragment patterns from about 63 % (137 of 217) in the standard PCR method to about 34 % (70 of 207) under the modified protocol, suggesting that just a low level of PCR cycling (5 cycles after 15 cycles) can significantly reduce the formation of chimeric DNA in the final PCR products. Phylogenetic analysis of 81 groups with distinct RFLP patterns that were obtained using the modified PCR method revealed that the clones were affiliated with at least 11 phyla or classes of the domain Bacteria. However, the analyses of 327 colonies, which were grouped into just 41 distinct types by RFLP analysis, showed that they could be classified into five major bacterial lineages: ${\alpha},\;{\beta},\;{\gamma}-$ Proteobacteria, Actinobacteria, and the phylum Bacteroidetes, which indicated that the microbial community yielded from the cultivation-based method was still much simpler than that yielded from the PCR-based molecular method. In this study, the discrepancy observed between the communities obtained from PCR-based and cultivation-based methods seems to result from low culturabilities of bacteria or PCR bias even though modified culture and PCR methods were used. Therefore, continuous development of PCR protocol and cultivation techniques is needed to reduce this discrepancy.

• 한국멀티미디어학회논문지
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• 제16권7호
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• pp.802-809
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• 2013

본 논문에서는 프로그래밍 언어에 정의되는 지정자와 키워드가 프로그램 코드 상에서 연속적인 패턴으로 나타나게 될 때, 해당 연속 패턴들의 빈도와 길이를 측정하여 두 코드 사이의 유사성을 측정하는 기법을 제안한다. 또한, 이러한 분석 결과를 정형적 개념 분석 기법을 이용하여 가시화하는 기법을 제안한다. 제안 기법은 기존의 유사도 측정 기법에서는 고려하지 않았던 단어 인접성을 유사도 측정에 반영한다. 함수 단위로 지정자와 키워드 패턴을 이용하여 함수의 호출 순서나 수행 순서에 상관없이 표절을 탐지할 수 있다. 또한, 유사도 측정 결과는 정형적 개념 분석 기법을 이용하여 격자(lattice)로 시각화되어 사용자의 이해도를 높일 수 있다. 실험 결과 제안 기법은 96%의 표절 탐지 성공률을 보여주었다. 제안 기법은 프로그램 코드 뿐만 아니라 일반 문서의 분석에도 적용될 수 있다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 A
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• pp.130-132
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• 2002

Because of the continuous growth of energy consumption and also the tendency to site power lines and pipelines along the same route, the close proximity of power lines and buried metallic pipelines has become more and more frequent. Therefore there has been and still is a slowing concern about possible hazards resulting from the influence of power lines on metallic pipelines. Underground pipelines that run parallel to or in close proximity to power lines are subjected to induced voltages caused by the time-varying magnetic fields produced by the power line currents. The induced electro- motive force cause currents circulation in the pipeline and voltages between the pipeline and surrounding earth. This paper analyzes the induced voltage on the gas pipelines buried in parallel with 22.9kV distribution lines. Their magnitude depends on the length of parallelism and on the distance between distribution lines and pipeline.

• 대한기계학회논문집B
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• 제35권11호
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• pp.1213-1217
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• 2011

의료용 자극기는 병원과 가정에서 환자의 통증 경감 및 재활 치료를 위해 사용되는 의료기기이다. 전기자극기의 자극 펄스가 심장에 유입되면 부정맥 및 심실세동 등의 심각한 부작용을 초래할 수 있다. 의료용 자극기에서 공급하는 자극 펄스의 전달 거리를 측정하고 심장이 전기 자극의 위험 범위 밖에 위치하도록 자극의 크기 및 자극 부위를 제한할 필요가 있다. 전기 자극기는 초당 60 회의 빈도로 0.001Joule 전기 펄스를 가하도록 설계되었다. 전기 자극기의 성능 및 인체에 미치는 영향을 측정하기 위해 생리 식염수를 이용한 생체 조직의 모델을 제작하였고, 이를 이용하여 거리에 따른 전기장의 감쇄 정도를 측정하였다. 본 연구에서 개발한 전기 자극기를 동물실험에 적용한 후 심장 주변에 전기자극을 가했을 때 심장에서 나타나는 위험요인을 관찰하고 직류전류가 흘렀을 때 심장에서 나타나는 현상과 비교하였다.

• The Plant Pathology Journal
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• 제29권2호
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• pp.125-135
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• 2013

In agro-ecosystems worldwide, some of the most important and devastating diseases are caused by soil-borne necrotrophic fungal pathogens, against which crop plants generally lack genetic resistance. However, plants have evolved approaches to protect themselves against pathogens by stimulating and supporting specific groups of beneficial microorganisms that have the ability to protect either by direct inhibition of the pathogen or by inducing resistance mechanisms in the plant. One of the best examples of protection of plant roots by antagonistic microbes occurs in soils that are suppressive to take-all disease of wheat. Take-all, caused by Gaeumannomyces graminis var. tritici, is the most economically important root disease of wheat worldwide. Take-all decline (TAD) is the spontaneous decline in incidence and severity of disease after a severe outbreak of take-all during continuous wheat or barley monoculture. TAD occurs worldwide, and in the United States and The Netherlands it results from a build-up of populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. during wheat monoculture. The antibiotic 2,4-DAPG has a broad spectrum of activity and is especially active against the take-all pathogen. Based on genotype analysis by repetitive sequence-based-PCR analysis and restriction fragment length polymorphism of phlD, a key 2,4-DAPG biosynthesis gene, at least 22 genotypes of 2,4-DAPG producing fluorescent Pseudomonas spp. have been described worldwide. In this review, we provide an overview of G. graminis var. tritici, the take-all disease, Pseudomonas biocontrol agents, and mechanism of disease suppression.

• 대한의용생체공학회:의공학회지
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• 제27권5호
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• pp.237-244
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• 2006

This study proposes an approach to the performance improvement of EMG(Electromyogram) pattern recognition. MFCC(Mel-Frequency Cepstral Coefficients)'s approach is molded after the characteristics of the human hearing organ. While it supplies the most typical feature in frequency domain, it should be reorganized to detect the features in EMG signal. And the dynamic aspects of EMG are important for a task, such as a continuous prosthetic control or various time length EMG signal recognition, which have not been successfully mastered by the most approaches. Thus, this paper proposes reorganized MFCC and HMM-GMM, which is adaptable for the dynamic features of the signal. Moreover, it requires an analysis on the most suitable system setting fur EMG pattern recognition. To meet the requirement, this study balanced the recognition-rate against the error-rates produced by the various settings when loaming based on the EMG data for each motion.

• Journal of Trauma and Injury
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• 제24권2호
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• pp.89-94
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• 2011

Purpose: A few studies have assessed the factors affecting the prognoses for major trauma patients and those improving the circumstances when dealing with the trauma system. In that light, we analyzed factors, such as pre-hospital factors, the time to admission, the length of stay in the emergency department (ED) and emergency operation, influencing the outcomes for trauma patients. Methods: The patients who visited our emergency department from April 1, 2009, to February 29, 2011, due to major trauma were enrolled in the study. The inclusion criterion was a revised trauma score (RTS) < 7 or injury severity score (ISS) ${\geq}$ 16. We used reviews of medical records, to analyze each step of emergency medical care with respect to patients' sex, age, visit time and visit date. Continuous variables were described as a median with an interquartile range, and we compared the variables between the survival and the mortality groups by using the Mann-Whitney U test. Fisher's exact test was used for nominal variables. Using the variables that showed statistical significance in univariate comparisons, we performed a logistic regression analysis, and we tested the model's adequacy by the using the Hosmer-Lemeshow method. Results: A total of 261 patients with major trauma satisfied either the RTS score criterion or the ISS score criterion. Excluding 12 patients with missing data, 249 patients were included in this study. The overall mortality rate was 16.9%. Time to ED arrival, time to admission, time of ED stay, RTS, ISS, and visit date being a holiday showed statistically significant differences between the survival and the mortality groups in the univariate analysis. RTS, ISS, length of ED stay, and visit date being a holiday showed statistical significance in the multivariate analysis. Conclusion: The mortality rate did not show a significant relationship with the time to ED arrival, use of 119, on time to admission. Rather, it elicited a quite significant correlation with the trauma scoring system (RTS and ISS), the time of ED stay, and the visit date being a holiday.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.316-323
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• 2020

본 연구는 제초제저항성 GM 들잔디에서 발현된 PAT 단백질의 잠재적인 알레르기성 및 독성을 평가하기 위해 수행되었다. PAT 단백질의 in silico 분석에서 PAT 단백질은 알려진 알레르겐 또는 독성 단백질과 유사성을 보이지 않았다. PAT 단백질은 80개의 아미노산으로 이루어진 절편에 걸쳐 기지의 알레르겐과 35% 미만의 아미노산 서열 상동성을 보였고, 기지의 알레르겐과 연속한 8개의 아미노산에서 일치하는 서열도 확인되지 않았다. 또한 제초제저항성 GM 들잔디에서 발현된 PAT 단백질은 펩신 존재 하의 인공위액에서 매우 빠르게 분해되었고, GM 들잔디에서 발현된 PAT 단백질은 번역 후 수식(glycosylation)도 일어나지 않았음이 확인되었다. 경구투여 독성 시험에서는 체중 1 kg 당 4,000 mg 을 투여한 실험구에서도 PAT 단백질 투여 후 마우스에서 사망이나 독성 영향은 관찰되지 않았다. 이들 결과는 제초제저항성 GM 들잔디에서 발현된 PAT 단백질이 잠재적 알레르겐이나 독소로 작용할 가능성이 없음을 시사한다.

• 한국방재학회:학술대회논문집
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• 한국방재학회 2008년도 정기총회 및 학술발표대회
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• pp.135-138
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• 2008

As the procedure of the close examination of disaster impact at the initial stage of the beginning of development plans, the newly extablished Pre Disaster Impact Assessment Review Deliberation (PDIARD) system which have been introduced through the revision of the Natural Disaster Countermeasure Act-revised at August, 2005 is enforced for the purpose of the disaster prevention which caused during a development projects. From that time down to this day, the PDIARD system have been reviewed about 6,000 cases totally. However, the current the PDIARD system at the uppermost limit in the aspect of every Acts and operational problems is in need of sustaining supplementation at the present situation. To cope with this operational remedy related to the PDIARD system, this study deals in 3 major concerns. First, it is required to fix a criterion in detail because of a wide range of the subject plans of deliberation. Second, there is some ambiguity according to the area or length of the subjected administrative and development plans. So it should be excepted of the targeted plans which has few probability in disaster potential or vulnerability. Finally, the subjected plans need to be added as to the continuous urbanization and industrialization and the enactment or revision of the Acts related. This study focuses on the investigation and analysis to find out the improvement way about the main problem pending of the PDIARD system, consequently suggests a effective remedy and new categories, including future directions and detailed plans for operation.

• Tribology and Lubricants
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• 제30권3호
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• pp.177-182
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• 2014

To stop a high speed train running at the speed of 300 km/h, the disc brake for the train should be able to dissipate enormous kinetic energy of the train into frictional heat energy. Sintered pin-type metals are mostly used for friction materials of high speed brake pads. A pad comprises several friction pins, and the topology, length, flexibility, composition, etc. have a great influence on the tribological properties of the disc brake. In this study, the topology of the friction pins in a pad was our main concern. We presented the optimization of the topology of a railcar brake pad with nine-pin-type friction materials by thermo-mechanical contact analysis. We modeled the brake pad with/without a back plate. To simulate a continuous braking, the pad or friction materials were rotated at constant velocity on the friction surface of the disc. We varied the positions of the nine friction materials to compare the temperature distributions on the disc surface. In a non-optimized brake pad, the distance between two neighboring friction materials in the radial direction from the rotational center of the disc was not equal. In an optimized pad, the distance between two neighboring friction materials in the radial direction was equal. The temperature distribution on the disc surface fluctuated more for the former than the latter. Optimizing the pad reduced the maximum temperature of the brake disc by more than 10%.