Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Allergenicity and toxicity evaluation of the PAT protein expressed in herbicide-tolerant genetically modified Zoysia japonica

제초제저항성 GM 잔디에서 발현된 PAT 단백질의 알레르겐 유발 가능성 및 독성 평가

  • Jeong, Hye-Rin (Department of Biotechnology, Jeju National University) ;
  • Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kang, Ji-Nam (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Lee, Hyo-Yeon (Department of Molecular Biotechnology, College of Applied Life Sciences, Jeju National University)
  • 정혜린 (제주대학교 생명공학과) ;
  • 선현진 (제주대학교 아열대원예산업연구소) ;
  • 강지남 (제주대학교 아열대원예산업연구소) ;
  • 강홍규 (제주대학교 아열대원예산업연구소) ;
  • 이효연 (제주대학교 생명공학부)
  • Received : 2020.12.02
  • Accepted : 2020.12.07
  • Published : 2020.12.31
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Abstract

This study aimed to evaluate the potential allergenicity and oral toxicity of the phosphinothricin acetyltransferase (PAT) protein expressed in Zoysia japonica, a herbicide-tolerant genetically modified (GM) zoysiagrass. In silico analysis of PAT showed no similarities with any known allergenic or toxic proteins, with <35% amino acid sequence homology with known allergens across a length of 80 amino acids and no continuous eight amino acid identity with known allergens. The PAT protein expressed in Z. japonica degraded very rapidly in the simulated gastric fluid in the presence of pepsin, and, no glycosylation of PAT was observed. The oral toxicity test revealed no mortality or toxic effect in mice following PAT administration at 4,000 mg/kg body weight. Our findings indicate that the PAT protein expressed in Zoysia japonica does not exhibit allergenic or toxic properties.

본 연구는 제초제저항성 GM 들잔디에서 발현된 PAT 단백질의 잠재적인 알레르기성 및 독성을 평가하기 위해 수행되었다. PAT 단백질의 in silico 분석에서 PAT 단백질은 알려진 알레르겐 또는 독성 단백질과 유사성을 보이지 않았다. PAT 단백질은 80개의 아미노산으로 이루어진 절편에 걸쳐 기지의 알레르겐과 35% 미만의 아미노산 서열 상동성을 보였고, 기지의 알레르겐과 연속한 8개의 아미노산에서 일치하는 서열도 확인되지 않았다. 또한 제초제저항성 GM 들잔디에서 발현된 PAT 단백질은 펩신 존재 하의 인공위액에서 매우 빠르게 분해되었고, GM 들잔디에서 발현된 PAT 단백질은 번역 후 수식(glycosylation)도 일어나지 않았음이 확인되었다. 경구투여 독성 시험에서는 체중 1 kg 당 4,000 mg 을 투여한 실험구에서도 PAT 단백질 투여 후 마우스에서 사망이나 독성 영향은 관찰되지 않았다. 이들 결과는 제초제저항성 GM 들잔디에서 발현된 PAT 단백질이 잠재적 알레르겐이나 독소로 작용할 가능성이 없음을 시사한다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 차세대바이오그린21 연구사업(과제번호:PJ01368501) 및 2019년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임(No. 2019R1A6A1A11052070).

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