• ALGAE
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• v.33 no.1
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• pp.127-141
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• 2018

Low efficiency in microalgal biomass production was largely attributed to the low density of algal cell cultures. Though mutations that reduced the level of chlorophyll or pigment content increased efficiency of photon usage and thus the cell-culture density under high-illumination growth conditions (e.g., >$500{\mu}mol\;photon\;m^{-2}\;s^{-1}$), it was unclear whether algae could increase cell-culture density under low-illumination conditions (e.g., ${\sim}50{\mu}mol\;photon\;m^{-2}\;s^{-1}$). To address this question, we performed forward genetic screening in Chlamydomonas reinhardtii. A pool of >1,000 insertional mutants was constructed and subjected to continuous subcultures in shaking flasks under low-illumination conditions. Complexity of restriction fragment length polymorphism (RFLP) pattern in cultures indicated the degree of heterogeneity of mutant populations. We showed that the levels of RFLP complexity decreased when cycles of subculture increased, suggesting that cultures were gradually populated by high cell-culture density (HCD) strains. Analysis of the 3 isolated HCD mutants after 30 cycles of subcultures confirmed that their maximal biomass production was 50-100% higher than that of wild type under low-illumination. Furthermore, levels of chlorophyll content in HCD mutant strains were similar to that of wild type. Inverse polymerase chain reaction analysis identified the locus of insertion in two of three HCD strains. Molecular and transcriptomic analyses suggested that two HCD mutants were a result of the gain-of-function phenotype, both linking to the abnormality of mitochondrial functions. Taken together, our results demonstrate that HCD strains can be obtained through continuous subcultures under low illumination conditions.

• Journal of Navigation and Port Research
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• v.31 no.1 s.117
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• pp.1-6
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• 2007

The evaluation of safety of simulated maneuvers is frequently analysed by so called goal-line or point of interest in Korea. For the purpose of warning the risk in the proximity measure composed of only the goal-line analysis, this paper utilized Korea Institute of Maritime and Fisheries Technology(KIMFT) which houses a real-time, full-mission shiphandling simulator to examine the goal-line and in-length analyses in the outbound channel of Kwangyang port as an example. It used a 15,000 TEU container ship as a model under environmental conditions of the northwestly 26-knot wind and 2.2-knot ebb current. The result of two analyses showed the probability invading the channel boundary obtained by the goal-line analysis is a little greater than that of the in-length analysis. Therefore it was acknowledged that the proximity measure by the goal-line analysis alone may be followed by some risk. In addition, this paper was to suggest the closest distance to channel boundary from the ship's edge as one of proximity measures, instead of using the ship's deviation from the centerline of channel.

• Proceedings of the KSR Conference
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• 2003.10b
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• pp.385-390
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• 2003

Recently, use of Continuous Welded rail(CWR) is increased for structural, economical reason but new problem is caused accordingly and phenomenon that give threat in traveling by ship stability of train is led. According as rail is prolonged, excessive relative displacement and longitudinal force can happen to rail by temperature change and external force. Specially, buckling or fracture of rail can happen in railroad bridges because relative displacement by bridge and properties of matter difference between rail grows and additional axial force happens to rail by behavior of bridge. According to several study, longitudinal force of rail in bridge is influenced with ballast resistance, elongation length, boundary condition, stiffness of framework. Non-linear behavior of ballast acts by the most important factor in interaction between rail and bridge. Therefore, must consider stiffness of bridge construction with non-linear characteristic of ballast and stiffness of base for accuracy with longitudinal force calculation and analyze. In this study, perform material non-linear analysis for longitudinal force of CWR and three dimensional buckling analysis to decide buckling force.

• Journal of Korean Academy of Nursing
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• v.40 no.2
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• pp.298-305
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• 2010

Purpose: The objectives of this study were to determine the prevalence, incidence, and risk factors for postoperative surgical site infections (SSIs) after craniotomy. Methods: This study was a retrospective case-control study of 103 patients who had craniotomies between March 2007 and December 2008. A retrospective review of prospectively collected databases of consecutive patients who underwent craniotomy was done. SSIs were defined by using the Centers for Disease Control criteria. Twenty-six cases (infection) and 77 controls (no infection) were matched for age, gender and time of surgery. Descriptive analysis, t-test, $\chi^2$-test and logistic regression analyses were used for data analysis. Results: The statistical difference between cases and controls was significant for hospital length of stay (>14 days), intensive care unit stay more than 15 days, Glasgrow Coma Scale (GCS) score (${\leq}7$ days), extra-ventricular drainage and coexistent infection. Risk factors were identified by logistic regression and included hospital length of stay of more than 14 days (odds ratio [OR]=23.39, 95% confidence interval [CI]=2.53-216.11) and GCS score (${\leq}7$ scores) (OR=4.71, 95% CI=1.64-13.50). Conclusion: The results of this study show that patients are at high risk for infection when they have a low level of consciousness or their length hospital stay is long term. Nurses have to take an active and continuous approach to infection control to help with patients having these risk factors.

• Steel and Composite Structures
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• v.33 no.3
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• pp.403-432
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• 2019

Axially functionally graded (AFG) beams are a new class of composite structures that have continuous variations in material and/or geometrical parameters along the axial direction. In this study, the exact analytical solutions for the free vibration of AFG and uniform beams with general elastic supports are obtained by using Euler-Bernoulli beam theory. The elastic supports are modeled with linear rotational and lateral translational springs. Moreover, the material and/or geometrical properties of the AFG beams are assumed to vary continuously and together along the length of the beam according to the power-law forms. Accordingly, the accuracy, efficiency and capability of the proposed formulations are demonstrated by comparing the responses of the numerical examples with the available solutions. In the following, the effects of the elastic end restraints and AFG parameters, namely, gradient index and gradient coefficient, on the values of the first three natural frequencies of the AFG and uniform beams are investigated comprehensively. The analytical solutions are presented in tabular and graphical forms and can be used as the benchmark solutions. Furthermore, the results presented herein can be utilized for design of inhomogeneous beams with various supporting conditions.

• The korean journal of orthodontics
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• v.26 no.1 s.54
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• pp.17-32
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• 1996

It is important to understand the operating mechanism and force system of fixed appliance that most effective for individual tooth movement in various orthodontic appliances. The archwire system of fixed appliance is devided into 3 types, which is continuous arch, segmented arch and sectional arch. The last two types have longer interbracket distance and simple force operating points, so it is easy to control force system by operator. But the continuous arch has shorter interbracket distance and various bracket geometry, so it is hard to control and anaylze the force system. The purpose of this study was three dimentional force and moment analysis of continuous arch system by finite element method, which is similar situation to three dimentional elastic beam in structural engineering. Several sample form of various bracket geometry and artificial lower crowding typodont made by author were constructed, analyzed and compared each other. The results were as follows : 1. The force magnitude is linear proportional to the degree of displacement or tilting of the bracket. 2. The force magnitude is inversely non-linear proportional to the interbracket distance. 3. In three dimensional typodont model, while the force can be compared with that of the sample form in the area where adjacent bracket geometry is simple, the force is much more than the expected value in the area where adjacent bracket geometry is complex.

• International Journal of Precision Engineering and Manufacturing
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• v.5 no.4
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• pp.50-60
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• 2004

A very flexible beam can be used to model various types of continuous mechanical parts such as cables and wires. In this paper, the dynamic properties of a very flexible beam, included in a multibody system, are analyzed using absolute nodal coordinates formulation, which is based on finite element procedures, and the general continuum mechanics theory to represent the elastic forces. In order to consider the dynamic interaction between a continuous large deformable beam and a rigid multibody system, a combined system equations of motion is derived by adopting absolute nodal coordinates and rigid body coordinates. Using the derived system equation, a computation method for the dynamic stress during flexible multibody simulation is presented based on Euler-Bernoulli beam theory, and its reliability is verified by a commercial program NASTRAN. This method is significant in that the structural and multibody dynamics models can be unified into one numerical system. In addition, to analyze a multibody system including a very flexible beam, formulations for the sliding joint between a very deformable beam and a rigid body are derived using a non-generalized coordinate, which has no inertia or forces associated with it. In particular, a very flexible catenary cable on which a multibody system moves along its length is presented as a numerical example.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.86-94
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• 1999

A set of rice recombinant inbred lines was developed from a cross between a Tongil type variety, Nonganbyeo, and an indica variety, BG276, by the single seed descent method. The number of the lines in the population was 272. All the agronomic characters studied except ADV (alkali-digestion value) showed continuous variation among the RILs, implying that their inheritance mode should be quantitative. The patterns of the variation in the RILs were either normal or skewed distribution. ADVs of RILs were segregated into two groups with 1:1 ratio, indicating that ADVs in this KIL population might be controlled by one major gene. Transgressive variations were also observed in all characters. Heritability values of the characters varied from 0.488 in brown/rough rice ratio to 0.895 in alkali-digestion value. In the analysis of genotypic and phenotypic correlations, the character of yield was positively correlated with 8 different agronomic characters. The number of panicles per hill was negatively correlated with culm length, panicle length, and number of spikelets per panicle. Grain length was positively correlated with grain width, grain thickness, grain length/width ratio, white belly, ADV, and amylose. However, grain length/width ratio was negatively correlated with grain width. White core was also negatively correlated with white belly and ADV.

• Journal of Chest Surgery
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• v.56 no.6
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• pp.374-386
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• 2023

Background: The heightened morbidity and mortality associated with repeat cardiac surgery are well documented. Redo median sternotomy (MS) and minimally invasive valve surgery are options for patients with prior cardiac surgery who require mitral valve surgery (MVS). We conducted a systematic review and meta-analysis comparing the outcomes of redo MS and minimally invasive MVS (MIMVS) in this population. Methods: We searched PubMed, EMBASE, and Scopus for studies comparing outcomes of redo MS and MIMVS for MVS. To calculate risk ratios (RRs) for binary outcomes and weighted mean differences (MDs) for continuous data, we employed a random-effects model. Results: We included 12 retrospective observational studies, comprising 4157 participants (675 for MIMVS; 3482 for redo MS). Reductions in mortality (RR, 0.54; 95% confidence interval [CI], 0.37-0.80), length of hospital stay (MD, -4.23; 95% CI, -5.77 to -2.68), length of intensive care unit (ICU) stay (MD, -2.02; 95% CI, -3.17 to -0.88), and new-onset acute kidney injury (AKI) risk (odds ratio, 0.34; 95% CI, 0.19 to 0.61) were statistically significant and favored MIMVS (p<0.05). No significant differences were observed in aortic cross-clamp time, cardiopulmonary bypass time, or risk of perioperative stroke, new-onset atrial fibrillation, surgical site infection, or reoperation for bleeding (p>0.05). Conclusion: The current literature, which primarily consists of retrospective comparisons, underscores certain benefits of MIMVS over redo MS. These include decreased mortality, shorter hospital and ICU stays, and reduced AKI risk. Given the lack of high-quality evidence, prospective randomized control trials with adequate power are necessary to investigate long-term outcomes.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.77-86
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• 2011

Alcohol dehydrogenase (E.C.1.1.1.1) is an enzyme present in higher plants involved in the anaerobic fermentation pathway that catalyzes the reduction of pyruvate to ethanol, resulting in continuous $NAD^+$ regeneration. It also plays an important role in many plant developments including tolerance to anoxia condition. Here, a cDNA clone encoding alcohol dehydrogenase (ADH) was isolated from Chinese cabbage (Brassica rapa) seedlings. The gene named Bradh1 had a total length of 1,326 bp that contains a single open reading frame of 1,140 bp. The predicted protein consists of 379 amino acid residues with a calculated molecular mass of 41.17 kDa. Expression pattern analysis revealed a tissue-specific expressing gene in different tissues and strongly expressed in the shoot, roots and seeds of Chinese cabbage. Agrobacterium transformation of full-length cDNA Bradh1 into rice Gopumbyeo showed high efficiency. Furthermore, induction of ADH in transgenic rice enhanced tolerance to anaerobiosis stresses and elevated mRNA transcripts. The overexpression of Bradh1 in rice increases germination under anaerobiosis stresses, implying the possibility of developing new varieties suited for direct seeding or flood-prone rice field.