• Journal of Microbiology and Biotechnology
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• v.2 no.1
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• pp.26-34
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• 1992

The effect of induction temperature on fermentation parameters has been investigated extensively using Escherichia coli M5248[pNKM21], a producer of recombinant human interleukin-2 (rhIL-2). In this recombinant microorganism, the gene expression of rhIL-2 is regulated by the cI857 repressor and $P_L$ promoter system. The recombinant fermentation parameters studied in this work include the cell growth, protein synthesis, cell viability, plasmid stability, $\beta$-lactamase activity, and rhIL-2 productivity. Interrelationships of such fermentation parameters have been analyzed through a quantitative assessment of the experimental data set obtained at eight different culture conditions. While the expression of rhIL-2 gene was repressed at culture temperatures below $34^\circ{C}$ with little effect on other fermentation parameters, under the conditions of rhIL-2 production $>(36~44^\circ{C})$ the cell growth, plasmid stability, and $\beta$-lactamase activity were, as induction temperature was increased, more profoundly reduced. Although the rhIL-2 content in the insoluble protein fraction was maximum at $40^\circ{C}$, total rhIL-2 production in the culture volume was found to be highest at the induction temperature of $36^\circ{C}$. This was in contrast to the previously known optimum induction temperature of the P$_{L}$ promoter system $>(40~42^\circ{C})$.Explanations for such a discrepancy have been proposed based on a product formation kinetics, and their implications have been discussed in detail.l.

• Korean Journal of Poultry Science
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• v.26 no.1
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• pp.43-50
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• 1999

The features of special brand eggs are growing more and more diverse and it is hard to draw a clear distinction between these and ordinary (no-brand) eggs. Special brand eggs range from those with objectively recognizable characteristics to those given only an unsubstantial product image and price differentials are among them great, too. The relation between product features and prices is unclear. Special brand eggs are the commodity whose characteristics are the vaguest of all livestock products. Farm's brand eggs produce a high profitability to producers is they are directly sold to consumers, by, for example, home-delivery service. But if they are sold to supermarkets, etc., producer's (poultry farmer's) income becomes lower by the amount of distributors' margin, reducing the profitability substantially. Thus how to increase the ration of retailing is important for farmers to secure a high profit. The sales strategies of poultry are the combination of two elements, that is, new product development (product differentiation) and creation of new market. But it is difficult for special brand egg producers to develop products with clear characteristics (use value) distinct form ordinary eggs and so these producers depend on the factors of appearance, such as the color of egg shells and package. Special brand eggs manage to keep their marketable value by the combination of the few product features and product image. Thus NB eggs from feed producers have a great market-ability since they can take advantage of the power of patents and TV commercials. However, market differentiation affects profitability much more than product features and price gaps are very wide between directly sold and wholesaled eggs. The producers of special brand eggs have come to the turning point where they have to decide whether they will content with being the subcontractors for NB and PB eggs or they will continue to keep their independence in production and marketing.

• Resources Recycling
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• v.15 no.1 s.69
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• pp.28-36
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• 2006

The development of material separation technique for waste plastic recycling are the necessary situation restricted by law the reclamation and incineration of waste plastic after 2004, with enforcement of EPR (Extended Producer Responsibility) system. As the this study is the research on the development of electrostatic separation techniques for recycling of life circles waste plastic by development of charging material and charger, the separation efficiency can be improved. Therefore, we developed the charger and electrostatic separator to increase charging efficiency and material separation per object plastics, rising these equipments, we removed PVC up to $99\%$ from two kinds of mixed plastics. And in case of restricting PVC content such as PET, we developed the separation technique that can remove PVC up to $99.99\%$ from PET with PET recovery about $80\%$. Also, as we separated over $98\%$ for three kinds of mixed plastics, and then established material separation technique to increase recycling of plastic.

• Proceedings of the Korean Society of Crop Science Conference
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• 2002.05a
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• pp.53-57
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• 2002

Quality evaluation must be more developed in order to offer the sufficient information for producer, distribution centers buyer, consumer. There are many parameters which influence the rice quality and cooked rice. It is difficult to evaluate the quality of rice and cooked rice by only some parameters. In the case of rice quality evaluation in Korea, physicochemical inspection is performed by examining the minimum and maximum limits of brown rice recovery, moisture content, damaged kernel, and colored kernel as inspection standard. Marketing standard of rice defines the limits of perfect, white core and belly, colored, damaged kernels, and broken rice, classifying into special, excellent, and normal grades. As a research direction for the development of rice quality evaluation, establishment as parts of technical field, must be further developed as follows : more detailed measure of characters, search of unknown taste-related components, creation and grade classification of quality evaluation factors at each management stages of treatment after harvesting, evaluation as food material as well as cooking rice, method development for simple evaluation and establishment of equation for palatability. In the side of policy, the following concerns must be conducted: price discrimination in conformity to rice cultivar and grade under the basis of qualify evaluation method developed, fixation of head rice branding, and introduction of low temperature circulation.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2005.10a
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• pp.23-33
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• 2005

The development of material separation technique for waste plastic recycling are the necessary situation according to restrict by law the reclamation and incineration of waste plastic after 2004 year, pith enforcement of EPR (Extended Producer Responsibility) system. As the this study is the research on the development of electrostatic separation techniques for recycling of life circles waste plastic, it can improve separation efficiency according to development of charging material and charger. Therefore, we developed the charger and electrostatic separator to increase charging efficiency and material separation per object plastics, using these equipments, we removed PVC up to 99% from two kinds of mixed plastics. And in case of restricting PVC content such as PET, we developed the separation technique that can remove PVC up to 99.99% from PET with PET recovery about 80%. Also, as we separated over 98% for three kinds of mixed plastics, and then established material separation technique to increase recycling of plastic.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.10
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• pp.704-710
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• 2014

Biomass is considered an alternative energy which can solve an greenhouse gas problem like $CO_2$ which is a major contributor to global warming. The biomass can be converted to various energy sources through thermochemical conversion. In this study, a continuous gasifier was engineered for a wood biomass gasification. The biomass was used a waste wood. The experiments of $CO_2$ gasification were achieved as the gasification temperature, moisture content and input $CO_2$ concentration. The results showed that the yield of producer gas increased with an increasing the gasification temperature. The amount of the light tar increased due to the decomposition of gravimetric tar by the thermal cracking, and the char was confirmed pore development through the SEM analysis. The CO concentration was increased with an increased input $CO_2$ concentration from Boudouard reaction. Through the parametric screening studies, the hydrogen and carbon monoxide concentration were 32.91% and 48.33% at the optimal conditions of this test rig.

• Journal of the Korean Society of Costume
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• v.60 no.4
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• pp.45-57
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• 2010

The costume in musical is an important visual factor that contributes to the level of stage completion by for the character and overall atmosphere of the whole play. Besides, as if it is has a fixed times in the drama, it has to produce to consider an opinion of producer to express the theme, atmosphere of a play and especially in the period adventure drama, to revive as it is historical dress, there is not creation at stage of today. This research is studied into the stage costume of the musical 'Jekyll and Hyde' an adapted to express image of good and evil in the one character. For this research, it researched costume of Victoria period in England, study character of main actor by analysis of the play in an original work, examine the stage costume of the musical 'Jekyll and Hyde' performed in the inside and outside of the country. In the musical stage costume, by character difficult of stage costume, factor of design feature divided to good and evil and compare feature design of stage costume, research new design express method to consider musical acting, moreover through designing and producing the costume to be suitable for the content of the work. This research consists of research and actual production of work, study of documentary records, collection and analysis of overseas performances, news releases, video material and internet research were the methods of this study.

• Korean Journal of Veterinary Service
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• v.42 no.2
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• pp.67-71
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• 2019

A variety of livestock feed resources were used in Korean dairy farm due to a lack of the endemic feed. However, there is inadequate real farm data to support farmers' decisions on the choice of options. The main objective of this study was to evaluate the nutritional value of total mixed ration (TMR) as well as the metabolic diseases status in Korean dairy farms. TMR samples were collected from nine feed companies and eight selected self-formulated by the dairy farms. The nutrient contents were examined by AOAC methods. The frequency of metabolic diseases such as ketosis and hypocalcemia were surveyed. The average moisture content was 36.2% although the min. and max. value were varied from 21.7% and 50.6% among farms. The mean${\pm}$standard deviation of crude fiber (CF), crude ash (CA), ether extract (EE), and crude protein (CP) were $21.4{\pm}2.5$, $4.6{\pm}0.4$, $3.2{\pm}0.5$ and $9.8{\pm}1.7$, respectively. However, the average ADF and NDF was $17.3{\pm}3.7$ and $31.0{\pm}5.7$, respectively. The compositions of TMR were varied significantly among the dairy farms. The frequency of clinical Ketosis (CK), subclinical ketosis (SCK) and hypocalcemia were higher in early lactation period with 4.5%, 11.0% and 3.0%, respectively. Also, the frequency of SCK was higher than CK and hypocalcemia throughout the lactation. Periodic TMR nutrient analysis based on herd production or physiology change would maximize the effects of TMR feeding. Furthermore, the study results would be useful to the farm practitioner and producer for their farm management.

• Journal of Korea Society of Industrial Information Systems
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• v.15 no.3
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• pp.23-32
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• 2010

U-healthcare provides people with many conveniences and keeps their health. As facility centered medical environment is being changed to patient centered one, however, various problems are emerging. These problems range from institutional problems to technological, producer related, and user related ones. The researcher took interested in problems originating from users, in particular, elderly patients and guardians taking care of an elderly patient. The main focus of this study is the direction of serious contents design for training skills in HMI of U-healthcare equipment and developing emergency coping abilities. In this study, I started from research on elderly cognitive psychology and elders' motor reactions and interconnected the characteristics of various digital content forms. The results of this study are expected to be useful for the rapidly expanding elderly population and in the U-healthcare industry.

• Cartoon and Animation Studies
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• s.37
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• pp.335-360
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• 2014

An infatuation with webtoons is facilitated by the ultra-rapid internet infrastructures and the highest ratio of penetration of smartphones in the world. Recently, the webtoon has been the focus of a new cultural content and its development has been noticed both and home and abroad. This study aims to find the new role of webtoon as a socio-cultural communication media. The webtoon reflects the reality as it is and shows directly and minutely the social problems, even although the other media make it glorified or reduced. We observe the role of webtoon as a new communication media content that can make the place for social discussion within several generations and social stratums, in which the producer and the consumer can communicate directly and immediately. Two webtoons( and ) have been selected for a textual analysis. In two webtoons, we analyse the method of representation of the social problem in the technique of expression and storytelling. This helps us maintain that the webtoon deserves as the socio-cultural communication media and the social problems represented in webtoon must have been evaluated more valuable in a new perspective.