• Title/Summary/Keyword: contamination prevention

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Choosing Optimal STR Markers for Quality Assurance of Distributed Biomaterials in Biobanking

  • Chung, Tae-Hoon;Lee, Hee-Jung;Lee, Mi-Hee;Jeon, Jae-Pil;Kim, Ki-Sang;Han, Bok-Ghee
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.32-37
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    • 2009
  • The quality assurance (QA) is of utmost importance in biobanks when archived biomaterials are distributed to biomedical researchers. For sample authentication and cross-contamination detection, the two fundamental elements of QA, STR genotyping is usually utilized. However, the incorporated number of STR markers is highly redundant for biobanking purposes, resulting in time and cost inefficiency. An index to measure the cross-contamination detection capability of an STR marker, the mixture probability (MP), was developed. MP as well as other forensic parameters for STR markers was validated using STR genotyping data on 2328 normal Koreans with the commercial AmpFlSTR kit. For Koreans, 7 STR marker (D2S1338, FGA, D18S51, D8S1179, D13S317, D21S11, vWA) set was sufficient to provide discrimination power of ${\sim}10^{-10}$ and cross-contamination detection probability of ${sim}1$. Interestingly, similar marker sets were obtained from African Americans, Caucasian Americans, and Hispanic Americans under the same level of discrimination power. Only a small subset of commonly used STR markers is sufficient for QA purposes in biobanks. A procedure for selecting optimal STR markers is outlined using STR genotyping results from normal Korean population.

A Study on the Introduction of Pre-management System to Prevent Soil Contamination by Chemical Accident (화학사고시 토양오염 사전관리제도 도입을 위한 국내외 제도 분석 및 시사점)

  • Yoo, Keunje;Yang, Jihoon;Hwang, Sang-Il
    • Journal of Soil and Groundwater Environment
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    • v.21 no.4
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    • pp.20-29
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    • 2016
  • Although a number of chemical accidents have been occurred in South Korea, the effective prevention act for soil contamination has not been established so far. To effectively protect soil contamination from chemical accidents, decision support laws and regulations are absolutely essential. Regarding this situation, this study was aimed at diagnosing problems in current chemical safety management and prevention and response system against chemical accidents through analyzing the domestic and foreign causes of chemical accidents and the accident response procedures and finally suggesting policy measures for solving those problems. In order to clarify management of soil contamination by chemical accident, this study suggests the establishment of chemical accident preparedness, response, and making of local chemical management law and policy. This law needs to be supported by a clear management framework to guide government officials and all other stakeholders in the management of soil contamination by chemical accident.

Remote Control of Pumping System for Underground Water Pollution and Running Dry Prevention Using Ubiquitous (유비쿼터스를 이용한 지하수 오염과 고갈방지를 위한 펌핑시스템의 원격제어)

  • Tack, Han Ho
    • Journal of Korea Society of Industrial Information Systems
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    • v.18 no.3
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    • pp.9-15
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    • 2013
  • This paper was development of remote controller of pumping system by using ubiquitous for underground water contamination prevention in the area of glasshouse facility. This paper automatically controls from to temperature and humidity for pumping sprinkler at water hanging cultivation. This prevents indiscreetive development of underground water, and prevents damage of environmental pollution without complementary measures in case of water lacked humble-void. The result of this research, confirms decrease of electrical fee, prevention of indiscreet underground water usage and its drying up thought optimum farm products management and pumping control system.

토양측정망과 특정토양오염관리대상시설 부지 등에서 토양오염조사의 효율성 제고를 위한 환경정책의 고찰

  • Park Yong-Ha;Park Sang-Yeol;Yang Jae-Eui
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2006.04a
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    • pp.11-15
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    • 2006
  • Attempts were made to increase an efficiency of soil contamination investigation systems (SCISs) including Soil Network and Special Soil Contamination Management Facility Sites in Korea. In order to increase low efficiencies resulting from inappropriate SCISs, possible policy suggestions are driven based on the results from problem findings of Korean policy and comparisons of policies on industrialized countries including United States, United Kingdom, Germany, the Netherlands and Japan. First, functions of Soil Environment Conservation Act (SECA) on liability should be updated and reinforced to initiate a soil contamination investigation process for stakeholders including an owner(s) or a responsible party(ies) of the potentially soil contamination sites positively. Second, appropriate SCISs should be emerged for implementing the Soil Network and Special Soil Contamination Management Facility Sites properly. Stakeholders for the potentially contaminated sites should easily access and raise the soil contamination issues, and soil contamination investigation implemented by liable and profit environment (consulting) companies should be encouraged. Third, the soil contamination reporting system of SECA needs to change legally responsible. Further more, public announcement system showing soil quality of a site which exceeds a certain scale would be considerable. Fourth, liable environment (consulting) companies should legally execute Soil Environment Assessment of SECA.

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Managing Soil Contamination in the United States: Policy and Practice

  • Small, Matthew C.
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.10a
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    • pp.58-69
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    • 2003
  • Soil contamination in the United States is managed using a risk-based decision making process. In other words, we don't ask, 'how much soil contamination can be cleaned up\ulcorner' Instead we ask, 'how much contamination can be safely left in place\ulcorner' The determination of 'safe' levels of contamination is based upon the potential for exposure and the toxicity of the contaminants of concern in soil. Potential for exposure is determined by evaluating potential exposure pathways from source to receptor given current or reasonably anticipated land use. Soil cleanup goals are then calculated for any complete exposure pathways based upon toxicity and the route of exposure. In some cases, institutional or engineering controls are also used to limit the potential for exposure. In order to prevent a continuous degradation of environmental quality, risk-based cleanup approaches must be combined with strong contamination prevention programs. In addition, alternative risk management approaches should be incorporated into an overall risk reduction strategy.erall risk reduction strategy.

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Effectiveness of Glove for the Prevention of Microbial Contamination during the Dental Clinical Practice (치과 임상실습에서의 장갑의 미생물 오염 차단 효과)

  • Jeong, Sun-Ju;Kim, Jung-In;Kim, Ji-Yeon;Ban, Se-Hee;Um, Ji-Young;Jeong, Moon-Jin;Lim, Do-Seon;Jeong, Soon-Jeong
    • Journal of dental hygiene science
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    • v.14 no.4
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    • pp.537-545
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    • 2014
  • We conducted a study to access the effectiveness of glove and washing hands for the prevention of microbial contamination during the dental clinical practice. Microbial numbers at unwashed hands and gloves were $9.938{\time}10^3(CFU)/ml$, those at washed with soap were $9.44{\time}10^3CFU/ml$ And those at washed with a typical liquid soap including disinfectant, Dettol (Reckitt Benckiser, Slough, SL1 3UH, UK) were $6.58{\time}10^2CFU/ml$. In case of unwashed hand and hand washing with soap had similar microbial numbers and in case of hand washing with Dettol showed a rapid decrease of microbial number. Hand washing with Dettol was effective to prevent microbial contamination than hand washing with soap. Also microbial numbers in all case wearing gloves decreased. It showed effectiveness of gloves for prevention of microbial contamination. In addition to microbial numbers showed a tendency to increase from 3 hours on wearing gloves, the change of new gloves after 3 hours on wearing is necessary to minimize the danger of microbial contamination.

Management Criteria of Underground Strage Tank for Prevention of Soil Contamination (토양오염방지를 위한 지하저장탱크의 관리 방안 연구)

  • Kim, Joon Hyun;Han, Young Han
    • Journal of Industrial Technology
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    • v.18
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    • pp.453-460
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    • 1998
  • The purpose of this study was to analyze their status of underground storage tank(UST) facilities and level of soil contamination, and to establish its management criteria. DB program was developed to analyze the correlation between specific characteristic of UST and level of soil contamination. For a suitable management of UST, leakage monitoring and inspection method of UST was investigated. Inspection period was established based on the leakage rate. The most dominant factor for leakage seemed to be caused by the corrosion. Therefore, main factor, construction method and installation year of UST, and corrosion protection system were suggested for optimal protection of UST. Considering the present management status of UST in Korea, inspection and management criteria of UST should be accomplished in term of contamination protection of leakage, and flexible regulation act should be introduced for each specific site.

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Prevalence of Mycotoxins and Their Consequences on Human Health

  • Omotayo, Oluwadara Pelumi;Omotayo, Abiodun Olusola;Mwanza, Mulunda;Babalola, Olubukola Oluranti
    • Toxicological Research
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    • v.35 no.1
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    • pp.1-7
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    • 2019
  • Mycotoxin contamination is a global phenomenon and causes a wide array of negative effects and other complications. This study focused on commonly found mycotoxins in Africa and the possible means of prevention or reduction of their contaminating effects. Mycotoxins are secondary metabolites of mold and fungi; they are generally toxic to living organisms. Hundreds of mycotoxins have been identified thus far, with some, such as aflatoxins, ochratoxins, trichothecenes, zearalenone, fumonisins, and patulin, considered agro-economically important. Several factors contribute to the presence of mycotoxins in food, such as climatic conditions, pest infestation, and poor harvest and storage practices. Exposure to mycotoxins, which occurs mostly by ingestion, leads to various diseases, such as mycotoxicoses and mycoses that may eventually result in death. In light of this, this review of relevant literature focuses on mycotoxin contamination, as well as various methods for the prevention and control of their prevalence, to avert its debilitating consequences on human health. Clear evidence of mycotoxin contamination is present in Africa, and it was therefore recommended that adequate prevention and control of these toxic substances in our food system should be encouraged and that appropriate measures must be taken to ensure food safety as well as the enhanced or long-lifespan of the African populace. Governments, research institutions, and non-governmental organizations should tailor the limited resources available to tackle mycotoxin prevalence, as these will offer the best prospects for successful development of a sustainable food system in Africa.

The Recognition Level of Food Contamination with Residual Pesticides and Hazardous Heavy Metals in Taejon Area (식품의 잔류농약 및 유해중금속의 오염인식도 -대전지역을 중심으로-)

  • 한장일;김성애
    • Korean Journal of Community Nutrition
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    • v.3 no.3
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    • pp.454-465
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    • 1998
  • This study was to investigate the recognition level of food contamination with residual pesticides and hazardous heavy metals from 365 male and female adults in Taejon area using questionnaires. Among the recognizing level of residual pesticides in overall foods, 69.1% were 'serious', 25.6% were 'average' and 5.3% were 'not serious'. Over 94.7% of the subjects recognized residual pesticides pollution infoods. for hazardous heavy metals in food, 47.8% responsed as 'serious', 40.5% as 'average' and 11.8 as ' not serious' . Over 88.3% of the subjects recognized contaminated pesticides seriously in fruits, 72.1% in vegetables and 51.7% in cereals, whereas 55.7% of the subjects recognized hazardous heavy metal contamination seriously in fruits, 53.4% in vegetables, 40.8% in fishes and shellfishes and 35.0% in seaweeds. The subjects recognized residual pesticides contamination more seriously in overall foods, cereal, potatoes and starches, bean, vegetables and fruits, whereas hazardous heavy metal contamination was recognized more seriously in fishes and shellfishes, and seaweeds food groups. Comparisons were shown based on individuial's occupation. Farmer, forester, iner and fisher showed the lowest recognizing level of food contamination in most food groups. The mean score of the dietary effect by mass media's information on food contamination from residual pesticides and hazardous heavy metals were 3.51±0.96 out of 5 points. By Duncan's multiple range test, sex, age, marriage, food cost per month, concerns about health and nutrition knowledge showed significant differences in the mean effect score at p<0.05. According to a pesticides contamination in several food groups were affected by food cost per month, mass media's information on food contamination, health status, and concerns about health, But a recognition level of hazardous heavy metals in food were affected by income and, food cost per month mass media's information on food contamination, health status, concern about health and nutrition knowledge. People who need to take extreme precautions of food contamination were in order of producers, government officials, homemakers, the consumer's association and consumers.

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Study on Inhibition of Microorganism Derived from Bovine Frozen Semen in In Vitro Culture of Bovine Follicular Oocytes (소 난포란의 체외배양에 있어서 소 동결정액유래 미생물 억제에 관한 연구)

  • 이성학;정구민;이종호;가학현;임경순
    • Korean Journal of Animal Reproduction
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    • v.19 no.1
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    • pp.1-7
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    • 1995
  • This study was accomplished to illuminate factors of contamination of microbes in the culture medium and effect of antibiotics on prevention of contamination in the medium when bovine follicular oocyte was matured, fertilized and developed in vitro. 1. When washed or unwashed semen diluted with TCM 199 was incubated for 24∼72hr, contamination was come out. 2. When diluted semen with TCM 199 which has penicillin, streptomycin, gentamycin, kanamycin or nystatin was incubated for 24∼72hr, contamination was not come out only in kanamycin. 3. When imported semen which was diluted in TCM 199 with penicillin, streptomycin, gentamycin, kanamycin or nystatin was incubated for 24∼72hr, contamination was not come out in all treatments. 4. When semen which was diluted in BO, CZB, Ham's F10 or TCM 199 was incubated for 24∼72hr, kanamycin showed no contamination in all treatments, but gentamycin showed contamination in CZB, Ham's F10 and TCM 199. 5. When the semen diluted in BO was moved at 24hr after incubation into BO and incubated for 72hr, contamination was not come out, but when it was moved into the TCM 199 and incubated for 72hr, contamination was come out at 48 to 72hr of incubation. 6. When the semen diluted in BO, BO+BSA or BO+FBS containing gentamycin, kanamycin or nystatin was incubated for 24∼72hr, the diluted semen in BO or BO+BSA showed no contamination in all antibiotics but the diluted semen in BO+FBS showed no contamination only in kanamycin. 7.The Pseudomonas cepacia, Serratia liquefaciens, Klebsiella pneumaniae was respectively isolated in the semen of A, B, and C bull and the microbes are highly affected by amikacin, tobramycin and kanamycin. 8. When bovine folicular oocyte was in vitro matured, fertilized and developed in the simple medium with kanamycin, 26.6% was developed to over 32cell stage embryo.

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