• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.6
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• pp.1391-1396
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• 2011

A way to increase productivity in agriculture that is labor-centered industry is to graft IT technology. Today, many technologies in ubiquitous computing are deployed in all areas of society such as traffic control, automotive manufacturing, construction, defence, healthcare and clinical services. These IT technologies is gaining more attention as a fusion technology among traditional industries. To successfully build ubiquitous agriculture environment, it needs optimized core technology development for agriculture that includes sensor node H/W, middleware platform, routing protocol and agriculture environment application services. To achieve accurate botany growth environment management, we propose a green growth environment management system using environmental factor monitoring sensor and biological information sensors in greenhouse. By using our proposed system, it is expected to realize fusion complex agriculture technology with low cost.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.4
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• pp.371-377
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• 2013

Fishing boats more than 5 tons is obliged to VHF DSC by Fishing Vessels Act and Vessel Safety Act. The owner of the fishing vessel is equipped to the automatic position reporting device in accordance with the Notice of the Minister of Land, Transport and Maritime Affairs to regulations, shall be ensure to navigations safety and in order to respond quickly in the event of maritime accidents on fishing vessels. East sea set up to start in 2012, which is now underway the annual install plain to the yellow sea and the south sea for VHF coast stations. It is web-based remote operation of DSC on the remote control and monitoring in Fishery Information Communication Station for the coastal VMS construction project. All fishing vessels is VHF DSC in conjunction with the GPS that location information transmitted to the coast station. automatically by the DSC call. This paper has been studied on the communications coverage set up and traffic operation for realization a roaming service by navigation route tracking and RSSI techniques in parallel algorithm refer to VHF DSC coast stations in east sea.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.34
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• pp.109-131
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• 2007

This paper has focusing CheongHaeJin's maritime trading activities between Korea, China and Japan in the 9th century. In operation of CheongHaeJin which Chang BoGo was given a key role, CheongHaeJin creates three nation's wealth in triangular trade among Korea, China and Japan. And also, CheongHaeJin's contribution is considerable to the maritime trade development of Northeast Asia through establishing trading advance base in China and Japan, and organizing Shilla's people in China. Chang BoGo justified the control over small business groups of the west and south sea of Korea and the east and south sea of China by keeping pirates away His trade groups controlled foreign trade of three countries: Shilla, the Dang Dynasty of China and Japan. They connected Persia, India, Southeast Asia, and China. CheongHaeJin's key success factors of the maritime activities are summarized as follows; There is a possibility of searching that successful factor from the people of operator of CheongHaeJin. Based on oceanic adventurous spirit with character and progressive thinking could complete the rally of sea influence and composition of fleets. Secondly, the success factor is the excellent operational ability and leadership which learned in the Dang Dynasty of China. Thirdly, In 9th century, International political context was suitable for CheongHaeJin's construction and operation. Such political circumstances had given to CheongHaeJin remunerative position. Finally, Although central government could not maintain the sea traffic securities, Chang BoGo's ocean trading fleets guaranteed the safe fishing industry of people and security of sea traffic.

• Tunnel and Underground Space
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• v.28 no.2
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• pp.142-155
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• 2018

The ${\bigcirc}{\bigcirc}$ mine has irregularly developed stratum around the ore body. The purpose of this study is to array irregular stratum thickness systematically for effective roof bolting and to implement a supporting system corresponding to it. The number of 81 cases combined with stratum thicknesses was limited to 9 cases by robust design. For each case, the load height which can act as a roof load was determined by the characteristics of stratum and RMR. The load range due to the load height is calculated assuming block shaped and arch shape. The support load of the roof bolt was considered as the average load of the two methods. Numerical analysis results of the support design showed that the cable bolt was more effective for the roof supporting fully grouted than the anchoring type. As a result of the construction, it was possible to control the roof, but all of the roof was gradually sinking downward due to the deformation of the side wall of the mine tunnel.

• BMB Reports
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• v.38 no.1
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• pp.41-48
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• 2005

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, which could infect Hz-AM1 cells and generate recombinant HaNPV (rHa-Bar) containing the barnase gene, a ribonuclease gene from Bacillus amyloliquefaciens. Since the expression vector carrying barnase gene cannot replicate in the absence of barstar, a specific inhibitor of barnase, we constructed a new cotton bollworm cell line (AM1-NB) using the marker rescue method. In AM1-NB barstar was integrated into the cellular chromosome to sustain the replication of rHa-Bar. To screen out recombinant HaNPV for potential use as biopesticide, Hz-AM1 and AM1-NB cell lines were infected with rHa-Bar, respectively. The results obtained indicate that Viral progenies in AM1-NB were 23 and 160 times greater than those in Hz-AM1 48 h and 72 h after infection, respectively. With additional insertion of the polyhedron gene from AcNPV (Autographa californica nuclear polyhedrosis virus) into the Hanpvid genome, rHa-Bar regained the polyhedron phenotype and its pest-killing rate greatly improved. Toxic analysis showed that the lethal dosages ($LD_{50}$) and the lethal time(s) ($LT_{50}$) of rHa-Bar were reduced by 20% and 30%, respectively, compared to wt-HaNPV in the third instar larvae of cotton bollworm. This study shows that in AM1-NB barnase can be effectively produced and used as pest-killing agent for the biological control of cotton pests.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.05a
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• pp.132-136
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• 2007

In case of indoor gymnastics training floor, in view of its characteristics, since it is simultaneously required the related smooth communication between the coach and the player, also the acoustic performance regarding to the Clearness of Music, besides the sport activity, the consideration about the acoustic character has entered the stage as an indispensable element. On such viewpoint, on the object of the recently built dome-typed gymnastics training floor, after making the optimized acoustic design with the remodeling through acoustic simulation, by means of measurement and valuation on human's psychological(sensual) degree utilizing Auralization that enables to experience the virtual sound field at the stage of design, this thesis has attempted to survey of the acoustic satisfaction degree and its reaction about the gymnastics training floor. As the result of investigation about the research on the space of object, it could be known that the valuation regarding to the acoustic performance of 'After-Improvement' was distinctly more refined than that of 'Before-Improvement'. It is now considering that such result of the study can be utilized as the useful data which enables to improve the retrenchment effect of the construction cost as well as the acoustic capability, by means of the prediction control on the acoustic problem from the stage of design, for the occasion when the similar indoor sport gymnasium is planning to build for the near future.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.43-49
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• 2002

We have cloned cDNAs encoding toxin from the spider, Araneus ventricosus, and constructed a recombinant baculovirus expressing the insecticidal toxin. The cDNAs encoding toxin were cloned from the cDNA library of A. ventricosus. Sequence analysis of the cDNAs encoding the toxin of A. ventricosus revealed that the 240 bp cDNA for AvTox-1 and 192 bp cDNA for AvTox-2 have an open reading frame of 80 and 64 amino acid residues, respectively. The deduced protein sequence of the toxin genes of AvTox-1 and AvTox-2 was aligned to that of the snack Anemonia sulcata and scorpion Centruroides limpidus limpidus, respectively. Northern blot analysis indicated that AvTox-2 toxin gene showed a fat body-spe-cific expression pattern at the transcriptional level. Furthermore, we have explored the possibility of improving baculovirus by incorporating the A. vontricosus toxin gene into Bombyx mori nuclear polyhedrosis virus genome under the control of polyhedrin promoter, The AvTox-2 toxin gene was expressed as approximately 5.8 kDa band in the recombinant baculovirus-injected silkworm larvae. Bioassays with the recombinant virus expressing AvTox-2 on 5th instar silkworm larvae demonstrated a decrease in the time to kill $(LT_{50} days)$ compared to wild-type BmNPV-Kl $(LT_{50} 6.72 days)$ in the injection of 10 viruses. These results indicate that A. ventricosus toxin is a novel member of the spider toxin family, suggesting that the toxin gene can be used in recombinant baculoviruses to reduce insect feeding damage and increase the speed of insect kill.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1350-1355
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• 2004

The sprA and sprB genes, encoding the chymotrypsin-like proteases Streptomyces griseus protease A (SGPA) and Streptomyces griseus protease B (SGPB), and the sprT gene that encodes Streptomyces griseus trypsin (SGT) were cloned from S. griseus and were overexpressed in various strains of S. griseus. When the sprT gene was introduced into S. griseus, trypsin activity increased 2-fold in the A-factor deficient mutant strain, S. griseus HH1, and increased 4-fold in the wild strain, S. grise us IFO 13350. However, there was no detectable increase of chymotrypsin activity in the transformants of S. griseus with either sprA or sprB, in contrast to the results obtained from S. lividans as a heterologous host. To solve the negative gene dosage effects in S. griseus, either the sprA or the sprB genes with their own ribosome binding sites were linked to the downstream of the entire sprT gene, and the coexpression efficiency was examined in S. lividans and S. griseus. The transformants of S. lividans with either pWHM3-TA (sprT+sprA) or pWHM3­TB (sprT+sprB) showed 3-fold increase of trypsin activity over that of the control, however, only the transformant of pWHM3-TB demonstrated 7-fold increase in chymotrypsin activity, indicating that the pWHM3-TB has a successful construction for the overexpression of chymotrypsin in Streptomyces. When the coexpression vectors were introduced into S. griseus IFO 13350, the trypsin level sharply increased by more than 4-fold, however, the chymotrypsin level did not increase. These results strongly suggest that the overexpression of the sprA and sprB genes is tightly regulated in S. griseus.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1337-1345
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• 2005

Vibrio vulnificus is the causative agent of foodborne diseases such as gastroenteritis and life-threatening septicemia. Microbial pathogenicity is a complex phenomenon in which expression of numerous virulence factors is frequently controlled by a common regulatory system. In the present study, a mutant exhibiting decreased cytotoxic activity toward intestinal epithelial cells was screened from a library of V. vulnificus mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, an open reading frame, fexA, a homologue of Escherichia coli areA, was identified and cloned. The nucleotide and deduced amino acid sequences of the fexA were analyzed, and the amino acid sequence of FexA from V. vulnificus was $84\%\;to\;97\%$ similar to those of AreA, an aerobic respiration control global regulator, from other Enterobacteriaceae. Functions of the FexA were assessed by the construction of an isogenic mutant, whose fexA gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of fexA resulted in a significant alteration in growth rate under aerobic as well as anaerobic conditions. When compared to the wild-type, the fexA mutant exhibited a substantial decrease in motility and cytotoxicity toward intestinal epithelial cell lines in vitro. Furthermore, the intraperitoneal $LD_{50}$ of the fexA mutant was approximately $10^{1}-10^{2}$ times higher than that of parental wild-type. Therefore, it appears that FexA is a novel global regulator controlling numerous genes and contributing to the pathogenesis as well as growth of V. vulnificus.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.685-691
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• 1998

Baculovirus Hyphantrin. cunea nuclear polyhedrosis virus (HcNPV) insecticide containing the insecticidal protein (ICP) gene from Bacillus thuringiensis subsp. kurstaki HD1 was constructed using a lacZ-HcNPV system. The ICP ($\delta$-endotoxin) gene was placed under the control of the polyhedrin gene promoter of the HcNPV. A polyhedrin-negative virus was derived and named ICP-HcNPV insecticide. Then, the insertion of the ICP gene in the ICP-HcNPV genome was confirmed by Southern hybridization analysis. Polyacrylamide gel electrophoresis (PAGE) analysis of the Spodoptera frugiperda cell extracts infected with the ICP-HcNPV showed that the ICP was expressed in the insect cells as 130 kDa at 5 days post-infection. The ICP produced in the cells was present in aggregates. When extracts from the cells infected with the ICP-HcNPV were fed to 20 Bombyx mori larvae, the following mortality rate was seen; 8 larvae at 1 h, 10 larvae at 3 h, and 20 larvae at 12 h. These data indicate that the B. thuringiensis ICP gene was expressed by the baculovirus insecticide in insect cells and there was a high insecticidal activity. The biological activities of the recombinant virus ICP-HcNPV were assessed in conventional bioassay tests by feeding virus particles and ICP to the insect larvae. The initial baculovirus insecticide ICP-HcNPV was developed in our laboratory and the significance of the genetically engineered virus insecticides is discussed.