• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.119-126
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• 2015

Among the various human growth factors, epidermal growth factor (hEGF, consisting of 53 amino acids) has various effects on cell regeneration, stimulation of proliferation, migration of keratinocytes, formation of granulation tissues, and stimulation of fibroblast motility, which are important for wound healing. Owing to their multiple activities, EGFs are used as pharmaceutical and cosmetic agents. However, their low productivity, limited target specificity, and short half-life inhibit their application as therapeutic agents. To overcome these obstacles, we fused the collagen-binding domain (CBD) of Vibrio mimicus metalloprotease to EGF protein. About 18 or 12 amino acids (aa) (of the 33 total amino acids), which were essential for collagen-binding activity, were combined with the N- and C-termini of EGF. We constructed, expressed, and purified EGF (53 aa)-CBD (18 aa), EGF (53 aa)-CBD (12 aa), CBD (18 aa)-EGF (53 aa), and CBD (12 aa)-EGF (53 aa). These purified recombinant proteins increased the numbers of cells in treated specimens compared with non-treated specimens and control hEGF samples. The collagen-binding activities were also evaluated. Furthermore, CBD-hybridized hEGF induced phosphorylation of the EGF receptor. These results suggested that these fusion proteins could be applicable as small therapeutic agents in wound tissue healing.

• International Journal of Highway Engineering
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• v.20 no.4
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• pp.1-6
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• 2018

PURPOSES : The objective of this study is to evaluate of the effect of thermal segregation reduction in asphalt paving using material transfer vehicles (MTVs). METHODS : Asphalt paving using MTVs was carried out, and the paved surface temperature was measured using an infrared camera. The amount of thermal segregation was estimated from temperature variations. RESULTS : The transportation of hot mix asphalt (HMA) using dump trucks caused temperature segregation that persisted in the paving surface if an MTV was not used. The average temperature variation was 8.58% in paved surfaces where an MTV was not used. However, the temperature variation was 3.10%, 2.86%, and 4.53% for the base layer, inter-layer, and surface layer, respectively, when an MTV was used. CONCLUSIONS : The use of an MTV in asphalt paving reduces thermal segregation approximately 2.3 times in an asphalt mat via a remixing process and also allows for a smoother work process because the paver never needs to stop to receive HMA. However, MTV equipment without pre-heating devices requires careful temperature control during the warm up process at the MTV during construction in the winter.

• Journal of Energy Engineering
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• v.25 no.3
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• pp.59-65
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• 2016

MVR evaporation is a method of pressurizing the evaporating steam to raise its temperature with an electric compressor instead of burning fuel and reusing the heat source through the embraced heat exchanger to minimize energy use. MVR desalination system with wind power uses varying wind power instead of stable electricity and can flexibly control the volume of fresh water production. The present study introduces the design, construction and operation of a MVR desalination system of 30ton/day capacity. Experimental results, MVR compression ratio is higher than 1.5, temperature difference of the main heat exchanger is $5{\sim}7^{\circ}C$. This value shows the same performance as the designed value.

• Explosives and Blasting
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• v.18 no.2
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• pp.14-22
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• 2000

The estimation of proper prediction method and the alteration of transformation method of environmental regulation standard were carried out by measuring blasting noise in construction field. The correlation of scaled distance with sound pressure level were better than with sound level, but it was proved to be difficult to control blasting noise because the correlation factor was too 1ow. three methods to transform sound pressure levee to sound level were examined. The method is the transformation by correlation equation of sound pressure level and sound level which are measured at the same time, and simplified transformation of A-weighting network corresponding to dominant frequency, and the transformation of sound pressure level by FFT. There were many errors to transform. The best effective method is the transformation using correlation equation of sound pressure level and sound level which are measured at the same time.

• Progress in Superconductivity
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• v.1 no.1
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• pp.20-25
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• 1999

We constructed a high-$T_c$ scanning SQUID microscope (SSM) operating in the liquid nitrogen. We used a washer-type YBCO SQUID with inner and outer dimensions of $12{\mu}m$ and $36{\mu}m$, respectively, which was grown on the $SrTiO^3$ bicrystal substrate. The sample, rather than SQUID, was scanned using two stepping motors. We also developed readout electronics, stepping motor controller, and the software for system control and data display. We took images of various samples using our SSM and found that the spatial resolution is about $40{\mu}m$ and noise level is lower than $10^{-7}T/{\surd}Hz$ at 100 Hz and higher at lower frequencies. The noise level was much higher than that of a typical SQUID due to the other coupling from the electric parts. We present a simple argument on the inductive coupling between the sample and the SQUID which should be under-stood for the proper interpretation of the obtained images. By comparing the measured data with the simulation results the gap between the SQUID and the sample is estimated to be $40{\mu}m$.

• Journal of environmental and Sanitary engineering
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• v.17 no.1
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• pp.36-40
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• 2002

The purpose of study was to suggest an investigation of citizen's attitude on harmful insects in house. This study was consisted of four parts, such as basic investigation (housing shape, housing material, the period of house constructed, a cleaning condition), condition of kitchen, condition of living room condition of bathroom. The environmental pollution was increased by the development of industry and urbanization in modern. And also the clean of house has related with air quality such as pollutants and harmful insects etc. Because dwelling shape of urban come to be crowded, shut tightly and high buildings, the air quality of indoor was gradually polluted by several irregular cause. In one of housing pollution, the harmful insects was important any other than indoor air pollution. The problems of kitchen were the odor of food waste, discharge system of sewage and harmful insects. The important issues of living room were mold and construction materials, then important them of bathroom were easing nature and discharge system of sewage. A kind of the harmful insects in house investigated cockroach> mosquito> ant>fly. Finally, it appeared that the control of harmful insects can be applied to the improvement of indoor air pollutant which are difficult to be controlled in house.

• Transactions of Materials Processing
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• v.18 no.3
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• pp.210-216
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• 2009

Springback, an elastic material recovery after the unloading of stamping tools, causes variations and inconsistencies of final part dimensions. Recently, narrow and long surround molding parts around door frame are applied to high grade automobiles, but there are great difficulties in their die development, construction and tryout because of several springback modes including vertical movement and twist during flange forming process of them. So it is very important to predict springback's quantities of a surround molding part and, moreover, to compensate the die for them adequately, when they can't be corrected by the restrike operation. This paper presents a case study based on the die design for a surround molding part made of stainless steel. The forming and springback predictions, carried out using PAMSTAMP 2G, are reported and compared with the measurement data of the prototype. The predicted springback results were acceptable, so the processes of compensating die using Automatic Die Compensation Module of PAMSTAMP 2G were performed iteratively until the tolerances between the designed shape and the simulation data were satisfied.

• Tribology and Lubricants
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• v.36 no.5
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• pp.274-278
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• 2020

This study investigates the possibility of reducing squeal noise generated at the contact point between an elastomer and glass by using the properties of a magneto-rheological elastomer (MRE) whose stiffness changes with the application of a magnetic field. Previously, squeal noise was mainly observed in the unstable section caused by the weakening of friction due to velocity. Previous studies have shown that squeal noise decreases as the stiffness increases. Accordingly, this study is conducted to control the unstable area of the friction curve and to reduce the noise by inducing the stiffness change of the MRE by applying a magnetic field. The friction, vibration, and noise characteristics are measured using a reciprocating friction tester. The frequency ranges of vibration and noise measured with the accelerometer and sound sensor show similar results. When a magnetic field is applied to the MRE, there is significantly lower noise compared with the case without the application of the magnetic field. The average coefficient of friction decreases with the application of the magnetic field. The maximum coefficient of friction increases rapidly at the turning point and decreases when the magnetic field is applied. This shows that the mechanical properties of the MRE change due to the magnetic field, and the noise and friction coefficient also decrease.

• Proceedings of the KWS Conference
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• 2005.11a
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• pp.79-81
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• 2005

Bridges constructed recently are preferred to have long spans and simple structure details considering not only the function as bridge but scenic beauty, maintenance, construction term and life cycle cost, etc. Therefore, they require high performance steels like extra-thick plate steels and TMCP steels. A TMCP steel produced by themo-mechanical control process is now spot lighted due to the weldability for less carbon equivalent. It improved at strength and toughness in microstructure. Recently, the SM570-TMC steel which is a high strength TMCP steel whose tensile strength is 600MPa has been developed and applied to steel structures. But, for the application of this steel to steel structures, it is necessary to elucidate not only the material characteristics but also the mechanical characteristic of welded joints. In this study, the characteristics of residual stresses in welded joints of SM570-TMC steel were studied through the three-dimensional thermal elastic-plastic analyses on the basis of mechanical properties at high temperatures obtained from the elevated temperature tensile test.

• Journal of Korean Society on Water Environment
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• v.29 no.1
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• pp.36-44
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• 2013

HSPF model based on BASINS was applied to analyze effects of watershed management measures for water quality conservation in the Hwaseong Reservoir watershed. The model was calibrated against the field measurements of meteorological data, streamflow and water qualities ($BOD_5$, T-N, T-P) at each observatory for 4 years (2007-2010). The water quality characteristics of inflow streams were evaluated. The 4 scenarios for the water quality improvement were applied to inflow streams and critical area from water pollution based on previous researches. The reduction efficiency of point and non-point sources in inflow streams was evaluated with each scenario. The results demonstrate that the expansion of advanced treatment system within wastewater treatment plants (WWTPs) and construction of pond-wetlands would be great effective management measures. In order to satisfactory the target water quality of reservoir, the measures which can control both point source and non-point source pollutants should be implemented in the watershed.