• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1362-1368
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• 2006

A new constitutive episomal expression vector, pGAPZ-E, was constructed and used for initial screening of eukaryotic target gene expression in Pichia pastoris. Two reporter genes such as beta-galactosidase gene and GFPuv gene were overexpressed in P. pastoris. The expression level of the episomal pGAPZ-E strain was higher than that of the integrated form when the beta-galactosidase gene was used as the reporter gene in P. pastoris X33. The avoiding of both the integration procedure and an induction step simplified the overall screening process for eukaryotic target gene expression in P. pastoris. Nine human protein targets from the Core 50, family of Northeast Structural Genomics Consortium (http://www.nesg.org), which were intractable when expressed in E. coli, were subjected to rapid screening for soluble expression in P. pastoris. HR547, HR919, and HR1697 human proteins, which had previously been found to express poorly or to be insoluble in E. coli, expressed in soluble form in P. pastoris. Therefore, the new episomal GAP promoter vector provides a convenient and alternative system for high-throughput screening of eukaryotic protein expression in P. pastoris.

• Steel and Composite Structures
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• v.20 no.1
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• pp.205-225
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• 2016

In this study the finite element method is utilized to predict the deflection and vibration characteristics of rectangular plates made of saturated porous functionally graded materials (PFGM) within the framework of the third order shear deformation plate theory. Material properties of PFGM plate are supposed to vary continuously along the thickness direction according to the power-law form and the porous plate is assumed of the form where pores are saturated with fluid. Various edge conditions of the plate are analyzed. The governing equations of motion are derived through energy method, using calculus of variations while the finite element model is derived based on the constitutive equation of the porous material. According to the numerical results, it is revealed that the proposed modeling and finite element approach can provide accurate deflection and frequency results of the PFGM plates as compared to the previously published results in literature. The detailed mathematical derivations are presented and numerical investigations are performed while the emphasis is placed on investigating the effect of the several parameters such as porosity volume fraction, material distribution profile, mode number and boundary conditions on the natural frequencies and deflection of the PFGM plates in detail. It is explicitly shown that the deflection and vibration behaviour of porous FGM plates are significantly influenced by these effects. Numerical results are presented to serve as benchmarks for future analyses of FGM plates with porosity phases.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.9
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• pp.117-126
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• 1998

For investigating rubber pad sheet metal forming process, the rubber pad deformation characteristics as well as the contact problem of rubber pad-sheet metal has been analyzed. In this paper, the behavior of the rubber deformation is represented by hyper-elastic constitutive relations based on a generalized Mooney-Rivlin model. Finite element procedures for the two-dimensional responses, employing total Lagrangian formulations are implemented in an implicit form. The volumetric incompressibility condition of the rubber deformation is included in the formulation by using penalty method. The sheet metal is characterized by elasto-plastic material with strain hardening effect and analyzed by a commercial code. The contact procedure and interface program between rubber pad and sheet metal are implemented. Inflation experiment of circular rubber pad identifies the behaviour of the rubber pad deformation during the process. The various form dies and scaled down apparatus of the rubber-pad forming process are fabricated for simulating realistic forming process. The obtaining experimental data and FEM solutions were compared. The numerical solutions illustrate fair agreement with experimental results. The forming pressure distribution according to the dimensions of sheet metal and rubber pads, various rubber models and rubber material are also compared and discussed.

• Advances in nano research
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• v.14 no.1
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• pp.45-65
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• 2023

This paper aimed to investigate the nonclassical size dependent free vibration behavior of regularly squared cutout viscoelastic nanobeams. The nonlocal strain gradient elasticity theory is modified and adopted to incorporate the viscoelasticity effect. The Kelvin Voigt viscoelastic model is adopted to model the linear viscoelastic constitutive response. To explore the influence of shear deformation effect due to cutout, both Euler Bernoulli and Timoshenko beams theories are considered. The Hamilton principle is utilized to derive the dynamic equations of motion incorporating viscoelasticity and size dependent effects. Closed form solutions for the resonant frequencies for both perforated Euler Bernoulli nanobeams (PEBNB) and perforated Timoshenko nanobeams (PTNB) are derived considering different boundary conditions. The developed procedure is verified by comparing the obtained results with the available results in the literature. Parametric studies are conducted to show the influence of the material damping, the perforation, the material and the geometrical parameters as well as the boundary and loading conditions on the dynamic behavior of viscoelastic perforated nanobeams. The proposed procedure and the obtained results are supportive in the analysis and design of perforated viscoelastic NEMS structures.

• Journal of Periodontal and Implant Science
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• v.29 no.3
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• pp.645-654
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• 1999

Interleukin-6(IL-6) stimulate osteoclast differentiation. $17{\beta}$-estradiol, 1,25-dihydroxyvitamin $D_3$(1,25-$(OH)_2D_3$) and interleukin-$1{\beta}$ inhibit or stimulate osteoclast differentiation by decreasing or increasing the synthesis of interleukin-6(IL-6) from stromal/osteoblastic cells, respectively. Periodontal ligament(PDL) cells reside between the alveolar bone and the cementum and have osteoblastic characteristics. To estimate the effect of $17{\beta}$-estradiol and 1,25$(OH)_2D_3$ on IL-6 production of PDL cells, PDL cells were treated with $17{\beta}$-estradiol or 1,25-$(OH)_2D_3$ in the absence or the presence of IL-$1{\beta}$. The concentration of IL-6 produced form PDL cells was determined by enzym linked immunosorbent assay(ELISA). In unstimulated PDL cells, we detected constitutive production of IL-6 at 1st and 2nd day. IL-$1{\beta}$ increased IL-6 synthesis at 1st day and 2nd day. $17{\beta}$-estradiol had no significant effect on the secretion of this cytokine, either constitutively or after stimulation with IL- $1{\beta}$(0.05 ng/ml). 1,25-$(OH)_2D_3$($10^{-8}M$) decreased not only constitutive IL-6 production but also IL-$1{\beta}$-induced IL-6 production at 2nd day. These results suggest that 1,25-$(OH)_2D_3$ may control IL-$1{\beta}$-induced osteoclast differentiation by decreasing IL-$1{\beta}$-induced IL-6 secretion of PDL cells.

• Molecules and Cells
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• v.27 no.1
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• pp.39-45
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• 2009

Ligand-dependent or independent oligomerization of receptor protein tyrosine kinase (RPTK) is often an essential step for receptor activation and intracellular signaling. The novel oncogene with kinase-domain (NOK) is a unique RPTK that almost completely lacks an ectodomain, expresses intracellularly and activates constitutively. However, it is unknown whether NOK can form oligomer or what function oligomerization would have. In this study, two NOK deletion mutants were generated by either removing the ectodomain ($NOK{\Delta}ECD$) or including the endodomain (NOK-ICD). Co-immunoprecipitation demonstrated that the transmembrane (TM) domain of NOK was essential for its intermolecular interaction. The results further showed that NOK aggregated more closely as lower order oligomers (the dimer- and trimer-sized) than either deletion mutant did since NOK could be crosslinked by both Sulfo-EGS and formaldehyde, whereas either deletion mutant was only sensitive to Sulfo-EGS. Removing the NOK TM domain (NOK-ICD) not only markedly promoted higher order oligomerization, but also altered the subcellular localization of NOK and dramatically elevated the NOK-mediated constitutive activation of extracellular signal-regulated kinase (ERK). Moreover, NOK-ICD but not NOK or $NOK{\Delta}ECD$ was co-localized with the upstream signaling molecule RAS on cell membrane. Thus, TM-mediated intermolecular contacting may be mainly responsible for the constitutive activation of NOK and contribute to the autoinhibitory effect on RAS/MAPK signaling.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.2
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• pp.55-64
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• 1984

Presented is a nonlinear constitutive model for progressive tensile fracturing of concrete. The model is incremental, path-dependent, and tensorialy invariant. The total strain tensor is assumed to be a sum of a purely elastic component and an inelastic component. The material is considered to contain weak planes of all directions which characterize the planes of the microcracks. A one-to-one functional dependence is assumed between the normal stress and the normal strain across each of the weak planes. The tangential stiffness of concrete is then derived form the principle of virtual work. The present theory can be applied to loading histories which are nonproportional or during which the principal directions rotate. Good agreement with the available direct tensile test data which cover strain-softening is demonstrated.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1643-1646
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• 2003

Drawing is a mechanical operation that attenuates thick material to an appropriate thickness for the next processing or end usage. When the input material has the form of a bundle or bundles made of very thin and long shaped wire or fibers, this attenuation operation is called "bundle drawing" or "drafting" Drafting is being used widely in manufacturing staple yarns. which is indispensable for the textile industry. However, the bundle processed by this operation undertake more or less defects in the evenness of linear density. Such irregularities cause many problems not only for the product quality but also for the efficiency of the next successive processes. Since long there have been many researches tying to find out factors affecting the irregularity of linear desity, to obtain optimal drafting conditions, to develop efficient measuring and analysis methods of linear density of bundle, etc., but there exists yet no fundamental equation describing the dynamic behavior of the flowing bundle during processing. In this research a mathematical model for the dynamic behavior of the bundle fluid is to be set up on the basis of general physical lows representing physical variables, i.e. linear density and velocity as the dynamic state of bundle. The conservation of mass and momentum balance was applied to the fluid field of bundle. while the movement of′ individual material was taken into account. The constitutive model relating the surface force and the deformation of bundle was introduced by considering a representative prodedure that stands for the bundle movement. Then a fundamental equations system could be simplified considering a steady state of the process. On the basis of the simplified model, the simulation was performed and the results could be confirmed by the experiments under various conditions.

• Structural Engineering and Mechanics
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• v.81 no.5
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• pp.617-631
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• 2022

In this paper the viscoelastic responses of four experimental steel-concrete composite beams subjected to highly variable environmental conditions are investigated by means of a finite element (FE) model. Concrete specimens submitted to stepped stress changes are also evaluated to validate the current formulations. Here, two well-known approaches commonly used to solve the viscoelastic constitutive relationship for concrete are employed. The first approach directly solves the integral-type form of the constitutive equation at the macroscopic level, in which aging is included by updating material properties. The second approach is postulated from a rate-type law based on an age-independent Generalized Kelvin rheological model together with Solidification Theory, using a micromechanical based approach. Thus, conceptually both approaches include concrete hardening in two different manners. The aim of this work is to compare and analyze the numerical prediction in terms of long-term deflections of the studied specimens according to both approaches. To accomplish this goal, the performance of several well-known model codes for concrete creep and shrinkage such as ACI 209, CEB-MC90, CEB-MC99, B3, GL 2000 and FIB-2010 are evaluated by means of statistical bias indicators. It is shown that both approaches with minor differences acceptably match the long-term experimental deflection and are able to capture complex oscillatory responses due to variable temperature and relative humidity. Nevertheless, the use of an age-independent scheme as proposed by Solidification Theory may be computationally more advantageous.

• Structural Engineering and Mechanics
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• v.81 no.1
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• pp.11-28
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• 2022

This paper considers the combination of cyclic and axial loads to investigate the hysteretic performance of H-section 6061-T6 aluminum alloy members. The hysteretic performance of aluminum alloy members is the basis for the seismic performance of aluminum alloy structures. Despite the prevalence of aluminum alloy reticulated shells structures worldwide, research into the seismic performance of aluminum alloy structures remains inadequate. To address this deficiency, we design and conduct cyclic axial load testing of three H-section members based on a reliable testing system. The influence of slenderness ratios and bending direction on the failure form, bearing capacity, and stiffness degradation of each member are analyzed. The experiment results show that overall buckling dominates the failure mechanism of all test members before local buckling occurs. As the load increases after overall buckling, the plasticity of the member develops, finally leading to local buckling and fracture failure. The results illustrate that the plasticity development of the local buckling position is the main reason for the stiffness degradation and failure of the member. Additionally, with the increase of the slenderness ratio, the energy-dissipation capacity and stiffness of the member decrease significantly. Simultaneously, a finite element model based on the Chaboche hybrid strengthening model is established according to the experiment, and the rationality of the constitutive model and validity of the finite element simulation method are verified. The parameter analysis of twenty-four members with different sections, slenderness ratios, bending directions, and boundary conditions are also carried out. Results show that the section size and boundary condition of the member have a significant influence on stiffness degradation and energy dissipation capacity. Based on the above, the appropriate material constitutive relationship and analysis method of H-section aluminum alloy members under cyclic loading are determined, providing a reference for the seismic design of aluminum alloy structures.