• Journal of Microbiology and Biotechnology
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• v.5 no.2
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• pp.61-67
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• 1995

To investigate the role of induction on CGTase production for alkalophilic Bacillus firm us var. alkalophilus H609, the constitutive mutants that form a halo around its colonies at non-inducible AG agar media containing amylose and glucose were selected. The selected constitutive mutants could produce CGTase in the range of 18.9 to 28.8 units/ml $\cdot A_{600}$ in the alkaline basal medium, and finally a constitutive mutant Bacillus firmus var. alkalophilus CM46 was selected. The constitutive nature of CM46 was also confirmed in protein level using SDS-PAGE. The effects of induction and catabolite repression for both parent strain Bacillus firmus var. alkalophilus H609 and constitutive mutant CM46 were also compared by adding soluble starch and glucose during cultivation. The selected mutant CM46 was a non-inducible but a catabolite regulated type mutant. Even though inductive regulation was released, the specific CGTase activity defined as CGTase activity per cell concentration was not increased compared with that of parent strain. The cell growth and CGTase production patterns of constitutive mutant Bacillus firmus var. alkalophilus CM46 were compared with the parent strain to identify CGTase production characteristics.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.05a
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• pp.233-236
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• 2004

The major objective of this paper is to clarify the effect of constitutive laws on bulk forming design based on the ideal flow theory. The latter theory is in general applicable for perfectly/plastic materials. However, its kinematics equations constitute a closed-form system, which are valid for any incompressible materials, therefore enabling us to extend design solutions based on the perfectly/plastic constitutive law to more realistic laws with rate sensitive hardening behavior. In the present paper, several constitutive laws commonly accepted for the modeling of cold and hot metal forming processes are considered and the effect of these laws on one particular plane-strain design is demonstrated. The closed form solution obtained describes a non-trivial nonsteady ideal process. The design solutions based on the ideal flow theory are not unique. To achieve the uniqueness, the criterion that the plastic work required to deform the initial shape of a given class of shapes into a prescribed final shape attains its minimum is adopted. Comparison with a non-ideal process is also made.

• Computers and Concrete
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• v.8 no.4
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• pp.371-388
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• 2011

In this paper, time-continuous constitutive equations for strain rate-dependent materials are presented first, among which those for the overstress and the consistency viscoplastic models are considered. By allowing the stress states to be outside the yield surface, the overstress viscoplastic model directly defines the flow rule for viscoplastic strain rate. In comparison, a rate-dependent yield surface is defined in the consistency viscoplastic model, so that the standard Kuhn-Tucker loading/unloading condition still remains true for rate-dependent plasticity. Based on the formulation of the consistency viscoplasticity, a computational elasto-viscoplastic constitutive model is proposed for the short fiber-reinforced fresh cementitious paste for extrusion purpose. The proposed constitutive model adopts the von-Mises yield criterion, the associated flow rule and nonlinear strain rate-hardening law. It is found that the predicted flow stresses of the extrudable fresh cementitious paste agree well with experimental results. The rate-form constitutive equations are then integrated into an incremental formulation, which is implemented into a numerical framework based on ANSYS/LS-DYNA finite element code. Then, a series of upsetting and ram extrusion processes are simulated. It is found that the predicted forming load-time data are in good agreement with experimental results, suggesting that the proposed constitutive model could describe the elasto-viscoplastic behavior of the short fiber-reinforced extrudable fresh cementitious paste.

• Geotechnical Engineering
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• v.12 no.4
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• pp.145-156
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• 1996

An implicit stress integration algorithm was formulated for implementing an aiusotorpic hardening constitutive model which has been based op the generalized isotropic hardening rule in nonlinear finite element analysis technique. the rate form of stress tensor was implicitly integrated using the generalized trapezoidal rule and the tangent stress-strain modulus was evaluated consistently with the nonlinear solution technique. As a result, it has been found that the nonlinear analysis with the anisotropic hardening constitutive model might be performed accurately and efficiently.

• Korea-Australia Rheology Journal
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• v.14 no.1
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• pp.33-45
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• 2002

Recent results obtained for the port-pom model and the constitutive equations with time-strain separability are examined. The time-strain separability in viscoelastic systems Is not a rule derived from fundamental principles but merely a hypothesis based on experimental phenomena, stress relaxation at long times. The violation of separability in the short-time response just after a step strain is also well understood (Archer, 1999). In constitutive modeling, time-strain separability has been extensively employed because of its theoretical simplicity and practical convenience. Here we present a simple analysis that verifies this hypothesis inevitably incurs mathematical inconsistency in the viewpoint of stability. Employing an asymptotic analysis, we show that both differential and integral constitutive equations based on time-strain separability are either Hadamard-type unstable or dissipative unstable. The conclusion drawn in this study is shown to be applicable to the Doi-Edwards model (with independent alignment approximation). Hence, the Hadamardtype instability of the Doi-Edwards model results from the time-strain separability in its formulation, and its remedy may lie in the transition mechanism from Rouse to reptational relaxation supposed by Doi and Edwards. Recently in order to describe the complex rheological behavior of polymer melts with long side branches like low density polyethylene, new constitutive equations called the port-pom equations have been derived in the integral/differential form and also in the simplifled differential type by McLeish and carson on the basis of the reptation dynamics with simplifled branch structure taken into account. In this study mathematical stability analysis under short and high frequency wave disturbances has been performed for these constitutive equations. It is proved that the differential model is globally Hadamard stable, and the integral model seems stable, as long as the orientation tensor remains positive definite or the smooth strain history in the flow is previously given. However cautious attention has to be paid when one employs the simplified version of the constitutive equations without arm withdrawal, since neglecting the arm withdrawal immediately yields Hadamard instability. In the flow regime of creep shear flow where the applied constant shear stress exceeds the maximum achievable value in the steady flow curves, the constitutive equations exhibit severe instability that the solution possesses strong discontinuity at the moment of change of chain dynamics mechanisms.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.2
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• pp.251-256
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• 2005

Up to now, a number of models have been proposed and discussed to describe a wide range of inelastic behaviors of materials. The fetal problem of using such models is however the existence of model errors, and the problem remains inevitably as far as a material model is written explicitly. In this paper, the authors define the implicit constitutive model and propose an implicit viscoplastic constitutive model using neural networks. In their modeling, inelastic material behaviors are generalized in a state space representation and the state space form is constructed by a neural network using input output data sets. A technique to extract the input-output data from experimental data is also described. The proposed model was first generated from pseudo-experimental data created by one of the widely used constitutive models and was found to replace the model well. Then, having been tested with the actual experimental data, the proposed model resulted in a negligible amount of model errors indicating its superiority to all the existing explicit models in accuracy.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.9 no.3
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• pp.191-197
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• 2009

Up to now, a number of models have been proposed and discussed to describe a wide range of inelastic behaviors of materials. The fatal problem of using such models is however the existence of model errors, and the problem remains inevitably as far as a material model is written explicitly. In this paper, the authors define the implicit constitutive model and propose an implicit viscoplastic constitutive model using neural networks. In their modeling, inelastic material behaviors are generalized in a state space representation and the state space form is constructed by a neural network using input-output data sets. A technique to extract the input-output data from experimental data is also described. The proposed model was first generated from pseudo-experimental data created by one of the widely used constitutive models and was found to replace the model well. Then, having been tested with the actual experimental data, the proposed model resulted in a negligible amount of model errors indicating its superiority to all the existing explicit models in accuracy.

• Korean Journal of Poultry Science
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• v.17 no.4
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• pp.275-280
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• 1990

Comosomal polymorphism involving constitutive heterochromatin has been reported in mu, pigs, mouse, horse, chicken and so on. The chromosomal polymorphism of Japanese quail which includes constitutive heterochromatin as well the chromosomes without banding treatment has now been found. Through the use of a general technique that permits visualization of chromosome morphology and heterochromatin, three chromosomal variants have been found among birds ； ＋/＋ homozygous from, ＋/－ heterozygous form and －/－ homozygous form in chromosome 4. This variants appear to be common in the randombred population and stably inherited in a Mendelian fashion. These results suggest that the variants would be useful as chromosomal markers for various types of cytogenetic studies.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1995.10a
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• pp.178-184
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• 1995

A new combined isotropic/kinematic and orthotropic hardening viscoplastic model is proposed which can account for not only differential orientations but also preferred orientations of grains in n metal at finite plastic deformations with an introduction of multiple spin (rate of rotation) concept within the general framework of the model, the effects of anisotropy and constitutive spins will be discussed in conjunction with a closed-form solution for simple shear in n rigid-plastic material, which will be used to simulate experimental data of Montheillet, et al. (1984) for fixed-end tortion tests at finite plastic deformations.

• Geomechanics and Engineering
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• v.1 no.4
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• pp.263-274
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• 2009

The modified Cam clay (MCC) model is used to study the response of virgin compressed clay in undrained compression. The MCC deviatoric stress-strain relationship is obtained in closed form. Elastic and plastic deviatoric strains are taken into account in the analysis. For the determination of the elastic strain components, both a variable shear modulus and constant shear modulus are considered. Constitutive relationships are applied to the well-known London and Weald clays sheared in undrained compression.