• Molecules and Cells
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• 제45권5호
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• pp.284-290
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• 2022

Exosome, a type of nanoparticles also known as small extracellular vesicles are gaining attention as novel therapeutics for various diseases because of their ability to deliver genetic or bioactive molecules to recipient cells. Although many pharmaceutical companies are gradually developing exosome therapeutics, numerous hurdles remain regarding manufacture of clinical-grade exosomes for therapeutic use. In this mini-review, we will discuss the manufacturing challenges of therapeutic exosomes, including cell line development, upstream cell culture, and downstream purification process. In addition, developing proper formulations for exosome storage and, establishing good manufacturing practice facility for producing therapeutic exosomes remains as challenges for developing clinical-grade exosomes. However, owing to the lack of consensus regarding the guidelines for manufacturing therapeutic exosomes, close communication between regulators and companies is required for the successful development of exosome therapeutics. This review shares the challenges and perspectives regarding the manufacture and quality control of clinical grade exosomes.

• 한국의류학회지
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• 제47권2호
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• pp.256-276
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• 2023

In South Korea, it has become popular for adolescent girls to wear makeup. From social perspectives on their makeup use, however, it remains controversial among parents, teachers, and even the girls. This study examined the status of adolescent girls' makeup usage, identified factors that influence their makeup use, and laid the groundwork for establishing a social consensus on makeup use among adolescent girls in South Korean society. Data from South Korean adolescent girls were collected and analyzed using MANOVA for repeated measures, confirmatory factor analysis, and structural equation modeling. Adolescent girls were found to wear less makeup at school than outside of school. Additionally, their makeup use was influenced by both positive (e.g., fashion leaders and social support from friends) and negative factors (e.g., low self-esteem). The findings of this study suggest that instead of strict bans against the use of makeup by girls, educational programs are needed to help adolescent girls grow up with healthy perspectives.

• 정보학연구
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• 제3권4호
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• pp.107-128
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• 2000

세계각국은 컨벤션산업을 21세기 성장을 주도하는 전략산업으로 집중 육성하고 있다. 그러나 우리 나라의 컨벤션 산업은 대형 컨벤션센터의 부재, 국제회의 전문인력의 부족, 국제회의 관련 업의 수도권집중, 항공노선의 부족, 관련업계와의 공조체제 부족, 국제회의정보 부족 등 많은 현안 문제가 있을 뿐 아니라 국제회의 전용시설이 전혀 없는 게 현실이다. 따라서 국가의 이미지 제고, 국제 교역 활성화, 지역개발촉진, 고용기회확대, 세수증대라는 광범위한 파급효과를 가져오는 컨벤션 산업의 육성을 위하여 국내 컨벤션 산업의 실태를 분석하고 그 문제점을 파악하여 개선방안을 제시하는데 목적을 둔다.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.650-655
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• 1998

The chromosomal DNA fragment from Phaffia rhodozyma CBS 6938 which is able to autonomously replicate in the yeast Saccharomyces cerevisiae was cloned on an integrative URA3 plasmid. Its minimal fragment exhibiting autonomously replicating activiy in the S. cerevisiae gave a higher frequency transformation efficiency than that found for centromere-based plasmid, and enabled extrachromosoma1ly stable transmission of the plasmids in one copy per yeast cell under non-selective culture condition. The 836-bp DNA element lacked an ORF and did not contain any acceptable match to an ARS core consensus. Sequence analysis, however, displayed a cluster of three hairpin-Ioop-sequences with individual $\triangle {G_{25}}^{\circ}C$ free energy value of -10.0, -17.5, and -17.0 kcal. $mor^{-l}$as well as a 9-bp sequence with two base pair mismatches to the S. cerevisiae/E. coli gyrase-binding site. This 836-bp sequence also included one 7-bp sequence analogous to the core consensus of centromeric DNA element III (CDEIII) of S. cerevisiae, but CDEIII-like 7 bp sequence alone did not give a replicative function in this yeast.

• Journal of Ecology and Environment
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• 제38권2호
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• pp.241-247
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• 2015

A participatory approach of local stakeholders is important to create a balance between nature protection and the sustainable use of natural resources in a nature reserve and its vicinity area. The zoning and management policy of biosphere reserves (BRs) is a good example of this idea, especially for island reserves. Yakushima was inscribed as a World Natural Heritage (WH) in 1993. Almost all of its residents did not remember that Yakushima was a BR. In addition, Yakushima has some problems in nature reserve management. Overabundance of the deer population is a critical threat to both agriculture and natural vegetation in the world heritage site. There are too many tourists visiting the WH site and Nagata-hama beach. The beach is a Ramsar site and one of the most important spawning grounds of sea turtles in the north Pacific. Now, residents and the municipality have decided to reactivate the Yakushima BR. Although the Ministry of the Environment of Japan has an initiative to manage the WH and the Ramsar site, the BR is managed by the municipality and local stakeholders. Local activities of both nature conservation and the sustainable use of natural resources are encouraged in the BR site. The islanders willingly included all areas of the island into the BR site. Scientists who have participated in the Society of Yakushimaology have played a significant role to build trust between local stakeholders and to seek feasible policies on the nature reserves. The concept of the "Yakushima Environmental Culture Village" is a good model for the extension plan of the Yakushima and Kuchinoerabujima BRs.

• 교육시설 논문지
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• 제21권1호
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• pp.43-53
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• 2014

In order to communicate each other we need both a system and contexts. Generally a system is called language and contexts are a common sense with a culture. If there is an absence of one of them it is impossible to understand each other. The concept of UPDP(User participating Design Process) is very similar to a communication. There must need both a system(formal tools) and a process to make consensus. Even though we already have two systems(KEBDI and PDAT) being developed by government affiliated research institutes these have not intimately related to a process to make consensus so far. In this respect the UPDP with using DQIfS of U.K. for a remodelling of Noryangjin elementary school(2013) is the most valuable because it is the first trying to communicate between laymen and professionals by menas both of a qualified system and of facilitations which have been developed from Namhansan UPDP(2001) in korea. The paper shows the process of UPDP including how to organise the members, detailed facilitation methods and the result of DQIfS analysis in U.K. Above all for the successful result the most important thing is the intimate relationship between the tool and facilitations at the beginning and user's will to build up.

• 비교문화연구
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• 제29권
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• pp.85-111
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• 2012

Zhong-lie-xiao-wu-yi(忠烈小五義), whose author was Shiyukun(石玉昆), is a Xia-Yi-Gong-An(俠義公案) novel in the late Qing Dynasty. This work published in 1890 when Emperor Guangxu(光緖) governed China. This work's author is Shiyukun, distribution books has an amender. The amender will be a shuoshuyiren (說書藝人). Zhong-lie-xiao-wu-yi is Zhong-lie-xia-yi-zhuan(忠烈俠義傳)'s a sequel, the story leads from Zhong-lie-xia-yi-zhuan. It is just the beginning of Zhong-lie-xiao-wu-yi is redundant. Zhong-lie-xiao-wu-yi was introduced to the late Chosun(朝鮮) Dynasty. This work was translated in Hangeul, Chosun's readers read Zhong-lie-xiao-wu-yi. This work's circulation is not clear, But this work's exciting story is interested in the readers. This work is characterized as follows: First of all, Zhong-lie-xia-yi-zhuan's charaters appear equally, the readers feels familiar. The readers like the familiar characters, because the readers read the book. The familiar characters can have a sense of speed in reading. Second, the story is continuous. Zhong-lie-xiao-wu-yi is narrated by connecting Zhong-lie-xia-yi-zhuan's story. Third, Zhong-lie-xiao-wu-yi was seeking an open ending. Classical novels prefer happy ending, this work is open ending, the expectations for the sequel became more doubled. The fourth, this work took advantage of the colloquial expressions. Zhong-lie-xiao-wu-yi is Huabenti(話本體) novel, took advantage of the spoken language. Suyu(俗語) and xiehouyu(歇後語) was represented in this work. Fifth, this work is formed a universal consensus. Ordinary people must empathize about xia-yi(俠義) and retribution, this work was well represented. Because the readers would have liked to this story.

• 한국과학교육학회지
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• 제44권3호
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• pp.273-299
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• 2024

이 연구는 국립해양생물자원관에서 운영하는 가족교육프로그램에 참여한 세 가족에 대하여 과학문화에 초점을 맞추어 가족팬덤 형성과정을 문화기술지방법론으로 수행한 질적연구다. 이는 서로 다른 환경의 세 가족이 가족교육을 통해 과학 활동을 일상적 문화실천 행동으로 나타난 향유-해독-변용의 과정을 거쳐 자생적 과학문화실천 형성 과정으로 가족팬덤 완성에 대하여 분석⋅요약된다. 이 연구의 주요 결과는 다음과 같다. 첫째, 가족과 함께 한 과학 활동이 유기적인 상호작용을 통해 유대감 강화와 과학문화에 대한 공감대가 확대된다. 둘째, 부모와 자녀는 일상생활에서 과학 관련 경험을 공유하며 과학적 소양인이 될 수 있는 독특하고 자신들만의 문화적 생활 속 문화공유형태로 실천했다는 것으로 나타났다. 이 연구는 완성형 자체로서의 과학문화 가족팬덤의 의미가 아닌, 완성형을 향해 가는 과정으로 종합적으로 밝혀보고자 했으며, 가족활동의 의미생산이 과학문화로 발전시킬 수 있는 사회문화적 함의를 갖는다.

• 한국콘텐츠학회논문지
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• 제16권11호
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• pp.223-239
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• 2016

본 연구는 국내 게임 규제 중 한국 사회에 가장 큰 영향력을 미치고 있는 '셧다운제'를 기준으로 게임 규제 대상자들의 반발심, 게임 이용 성향에 관하여 분석한 연구이다. 이용자들의 중독과 과몰입을 예방하기 위해 게임 규제 정책은 그 필요성이 인정되지만, 부가적인 사회적 비용이 크기 때문에 실효성을 높이는 것이 매우 중요하다. 본 연구의 검증 과정에서, 핵심 변수로 사용된 게임 이용 정도와 셧다운제 통제 여부는 반발심에 유의한 영향을 주는 것이 확인되었으나, 게임 이용 정도와 통제 여부의 상호작용에서 음(-)의 영향을 받는 의외의 결과가 나타났다. 또한, 규제 대상자들 중에 게임을 많이 하는 대상들은 게임을 또래 친구들과 어울리기 위하여 하는 경향이 있었으며, 친구들이 다른 것을 한다면 게임 대신에 다른 것을 할 것이라고 응답하였다. 이러한 결과는 청소년들에게 온라인 게임이라는 존재가 친구들과 소통하고 향유하는 문화공간이었음을 시사하며, 관련 게임 규제를 고안하는데 있어 규제 대상에 대한 심층적인 이해가 부족했음을 의미한다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.67-75
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• 2002

The human complement receptor type 1 (CR 1, C3 b/C4b receptor) is a polymorphic membrane glycoprotein expressed on human erythrocytes, peripheral leukocytes, plasma and renal glomerular podocytes, which consists of transmembrane and cytoplasmic domains with 30 repeating homologous protein domains known as short consensus repeats (SCR). CR1 has been used as an inhibitor for inflammatory and immune system for the past several years. Recently; it is reported that CRl was found to suppress the hyper-acute rejection in xeno-transplantation and can be used to cure autoimmune diseases. A soluble form of CRl, called sCRl, is a recombinant CRl by cleaving the transmembrane domain at C-terminus and has been expressed in Chinese Hamster Ovary (CHO) cells. Several purification methods for sCR1 from CHO cells have been reported, but most of them require complicated steps at high cost. Moreover, such methods are mostly performed under the pH condition apt to denaturing sCR1 and causes sCRl losing its activity. We here report a rapid and efficient method to purify sCR1 from CHO cell. The new method consists of a two-stage of cell culture by cultivating cells in serum medium followed by serum-free medium, and a two-stage of column purification by means of heparin and gel filtration column chromatography. By using this novel method, sCR1 can be purified in a simple and effective way with high yield and purity, furthermore, the purified sCR1 was confirmed to retain its activity to suppress the complement activation in vivo and ex vivo.