• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.72-77
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• 2009

Here, we demonstrate that the overexpression of the GroELS chaperone system, which assists the folding of intracellular proteins and prevents aggregation of its biological targets, can enhance the thermotolerance of Escherichia coli strains and facilitate the production of recombinant protein under thermal stress. The overexpression of GroELS led to an about 2-fold higher growth rate of E. coli XL-1 blue than control at $45^{\circ}C$ and induced the growth of the strain even at $50^{\circ}C$, although the growth was not sustained in the second-round culture. The effect of GroELS overexpression was also effective on other E. coli strains such as JM109, $DH5{\alpha}$, and BL21. Finally, we have shown that coexpression of GroELS allows us to produce recombinant protein even at $50^{\circ}C$, a temperature at which the protein production based on E. coli is not efficient. This study indicates that the employment of the GroELS overexpression system can expand the range of environmental conditions for E. coli.

• Journal of Korean society of Dental Hygiene
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• v.16 no.5
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• pp.741-750
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• 2016

Objectives: This study aims to identify dentists' opinions about the return to work of the dental hygienists with career interrupted. Convenience sampling was made of 22 dentists with the clinical experience (about 0.25%) of the 4,944 members of Gyeonggi-do Dental Association. Data were collected through an electronic survey using e-mails and telephone interviews and analyzed using the constant comparison method. Methods: Open coding was assigned to the initial data from the survey in an explicit language, and focusing on the types of their employment, working conditions, etc. Summarization and conceptualization were made of the second data in an implicit language. Results: Most of the dentists were found to have positive attitudes toward the return to work of career interrupted dental hygienists and hope to adjust their wages based on their job performance or after their probational period expires. Most of the dentists were found to have positive attitudes towards the hygienists' flexible working although their concerns about work efficiency and hospital atmosphere. This study is a qualitative study that describes dentists' opinions and presents the need for the career interrupted dental hygienists to return to work. Conclusions: The study proposed that the educational institutions should take into account the hygienists' opinions when implementing the programs for promoting their return to work and their evaluative studies.

• Journal of the Korean Society of Safety
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• v.35 no.6
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• pp.85-92
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• 2020

Wet Scrubber reacts the incoming pollutant gas with cleaning water (water + absorbent) to absorb pollutants and release the clean air to the atmosphere. Wet scrubbers and packed tower scrubbers using this principle are widely used in businesses that emit acid gases. In particular, in the etching process using hydrochloric acid (HCl), alkaline washing water (NaOH) having a pH of about 8 to 11 is used to absorb a large amount of acid gas. However, These salts are attached to the injection nozzle (nozzle), filling material (packing), and the demister (Demister), causing air pollution, human damage, and inoperability due to clogging and acid gas discharge. Therefore, In this study, an improvement plan was proposed to manage the washing water with pH 3~4 acidic washing water. The test method takes samples from the Wet Scrubber flue measurement laboratory twice a month for 1 year. Hydrogen chloride (HCl) concentration (ppm) was measured, and nozzle clogging and scale conditions were measured, compared, and analyzed through a differential pressure gauge and a pressure gauge. As a result of the check, it was visually confirmed that the scale was reduced to 50% or less in the spray nozzle, filler, and demister. In addition, the emission limit of hydrogen chloride in accordance with the Enforcement Regulation of the Air Quality Conservation Act [Annex 8] met 3 ppm or less. Therefore, even if the washing water is operated in an acidic pH range of 3 to 4, it is expected to reduce air pollution and human damage due to clogging of internal parts, and it is expected to reduce maintenance costs such as regular cleaning or replacement of parts.

• Korean Security Journal
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• no.6
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• pp.47-65
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• 2003

In Korea, Private security companies has steadily grown and amounts to 2,051 places as of November 30, 2002. Private security in korea is carrying out firm name role assignment to have held the police and public peace environment change factors for a standard faithfully, and protects social a little property that is a basic purpose, and it is spare no efforts in loss prevention. In spite of numeral increase of private security companies, private security companies have many problem. Moreover, they mostly did not have any remarkable in-house expertises in their own business under tough conditions. Under the unfavorable circumstances including insufficient investment and education in private security guard, there have been actually little further studies on private security business in practices. So this study mainly focused on addressing the installation security business managed by authorized companies, which amount to 96%(1,963 companies) of total 2,051 domestic security companies. Furthermore, the study formulated and modeled a series of business procedures in private security companies. A series of business procedures of private security companies can be modeled as follows : Setting of a business scope and aim market ${\Rightarrow}$ Marketing, Contact from customers(On-line or Off-line) ${\Rightarrow}$ Diagnosis of security target ${\Rightarrow}$ Submission of security operational plan ${\Rightarrow}$ Estimation of security operational plan ${\cdot}$ decision ${\Rightarrow}$ Contract ${\Rightarrow}$ Employment, selection of security guards ${\Rightarrow}$ Nomination of security guard instructors ${\Rightarrow}$ Education & training of security guards ${\Rightarrow}$ subscribe to insurance of damage liability ${\Rightarrow}$ Commitment and placement of security guards ${\Rightarrow}$ Establishment and preparation of security planning ${\Rightarrow}$ Field management and procurement of relevant security service.

• Asia-Pacific Journal of Business
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• v.15 no.1
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• pp.289-304
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• 2024

Purpose - Focusing on the FGI research method, the current status and issues of barrier-free tourism in Gangwon State were identified, and specific activation plans were derived Design/methodology/approach - This study conducted in-depth interviews with 20 experts related to the tourism industry. The issues, current status, and activation plan of barrier-free tourism in Gangwon State recognized by experts in tourism policy, tourism administration, tourism management, and tourism service industry were derived. Findings - First, the tendency of businesses to be promoted based on the non-disabled by not recognizing the weak as the main tourism demand group, second, confusion caused by the unification of terms related to barrier-free tourism, third, lack of finding tourism constraints subdivided according to the type and degree of disability, fourth, tourism information and evaluation indicators provided by prioritizing the convenience of suppliers rather than consumers, and fifth, the nature of the project focused on improving access to physical infrastructure. Subsequently, the results of the analysis of the plan to revitalize the barrier-free tourism industry in Gangwon State were as follows. First, education and programs to improve disability awareness appropriate to local conditions were activated, second, related information was unified and project sustainability was improved by creating a control tower for the barrier-free tourism industry at the provincial level, third, to overcome the employment crisis based on finding locally tailored jobs related to tourism care professionals, and fourth, to continuously secure quantitative and qualitative data and establish utilization plans. Research implications or Originality - First, it presents theoretical implications for related fields by providing issues and activation measures related to barrier-free tourism from the perspective of experts who are still lacking in research in academia. The practical implications are that based on the opinions of experts working in the tourism field, more specific issues of the barrier-free tourism industry in the province were presented, and policy and practical solutions were presented accordingly, suggesting realistic alternatives that can be carried out in the actual field.

• Korean Journal of Human Ecology
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• v.21 no.5
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• pp.957-973
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• 2012

This study analyzed consumer public complaint behaviors and the satisfaction of complaint handling among credit card users who availed of credit card services. Relatively little research has been done in this area, despite the obvious importance of understanding and improving credit card market conditions. The purpose of this study was to examine consumer compliant behaviors with a focus on public actions, such as voice responses and the third party actions among credit card users. With the goal of providing consumers with more positive expectations of credit card companies' complaint handling process, this study investigated the status of public actions and the negative effect of complaints on the overall satisfaction of post-complaint behavior toward credit card services. The responses from 1,000 credit card users were analyzed using descriptive analysis, factor analysis, multi-logit analysis, and Heckman selection estimate. The analysis provided three major results: (1) perceived service quality among credit card users was conceptualized into groups such as responsiveness, innovation, company, additional service, and fee, (2) perceived service qualities, age, residential area, employment status, and subjective economic status had significant effect on public compliant action behaviors, and (3) unidimensional factors resulting from post-complaint behaviors were analyzed and several variables, such as period of credit card use, average amount used, and perceived service quality had significant effects on the degree of satisfaction associated with complaint handling in terms of credit card services. Several implications and directions for further research are discussed.

• Journal of Family Resource Management and Policy Review
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• v.13 no.3
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• pp.31-57
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• 2009

The purpose of this study is to determine what factors affect the marital life of multicultural families. In-depth interviews were conducted with 15 immigrated females and their husbands living in Yongsan-Gu, Seoul, Korea, by open-ended questionnaire. The interviews took place on September 11, 2008, and October 11, 2008. The major findings can be summarized as follows: Through the content analysis of the informants' responses, three major factors were found to influence the successful adjustment of the married female immigrants: personal factors, familial support, and social support systems. Among the personal factors, the self-esteem of the married couples is relatively high. However, the factor of the economic living conditions of the multicultural families indicates some difficulty. Finally, considering the level of self-development of the female immigrants' husbands, an older husband was viewed more negatively than a younger one. In terms of familial support, the most important factor affecting the adjustment of a foreign wife is the trust of her husband and her relationship with the husband's family. Accordingly, educational programs are necessary to promote multicultural understanding and the couples' relations. In social support systems, the female immigrants experienced neglect, as far as multicultural prejudice in society. Therefore, in order to revitalize the self-esteem of immigrants, we need to reach out to multicultural families. The center for support of multicultural families provides opportunities to find training, upgrade education for employment, and expand their social network. In addition, it is necessary for the national public relations industry to recognize improvements regarding the adjustment of multicultural families.

• Analytical Science and Technology
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• v.14 no.6
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• pp.486-493
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• 2001

The effect of SIMS analysis conditions such as mesh grid, offset voltage and ion species on the in-depth profile for bismuth titanate thin film was examined in terms of charging effect and detection limit. The results shows that the use of offset voltage -40 V reduces the charging effect and the detection limit. The employment of mesh grid in sample preparation leads to the reduction of the charging effect in small amount, but deteriorate the detection limit. Utilization of primary $O^-$ ion for SIMS analysis of bismuth titanate thin film showed almost the same effect as using offset voltage -40 V. However, it takes approximately triple acquisition time than using $O_2{^+}$ ion due to the poor beam current of the source in the experiment.

• Korea Journal of Hospital Management
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• v.1 no.1
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• pp.37-55
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• 1996

This study attempts to analyze the factors affecting the intention of transferring to another hospitals among radiology technologists. 344 cases were reviewed in 5 university hospitals and 1 general hospital. Self-administered questionaire were given to study the socioeconomic characteristics, working conditions, job satisfaction level, and the reasons for transfer among the technologists. The major findings were as follows : 1. Job position and hospital characteristics had a statistically significant relationship with the intention of transferring to another hospital. 2. Those who were not satisfied with their salaries and promotional opportunity showed a higher tendency towards to transfer. 3. Those who were less satisfied with the opportunity for developing the personal ability and had the negative attitude on their job showed a higher tendency to transfer. 4. Those who did not sustain good relationship with their superiors and co-workers scored high on the tendency to transfer. 5. In the result of mutiple regression, recognition of radiation hazard, job satisfaction, satisfaction with salary levels, job attitude were significantly related to transfer. The above indicate that besides economic incentives, job satisfaction and organizational culture to promote their ability and form a good relationship with organization members were very important to decrease the intention of transfer. Since these results represent only 6 hospitals from a limited area, more hospitals nationwide, especially small and medium-sized institutions where there is a high turnover rate of employment, need to be examined in order to investigate the various factors that affect the intention of transferring.

• Journal of the Korean Data and Information Science Society
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• v.24 no.4
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• pp.701-712
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• 2013

The e-learning industry status survey statistic provides information about the actual conditions of supply and demand of the e-learning industries. NIPA (National IT Industry Promotion Agency) has published the annual report of the survey results since 2004. Due to the 9th version of the KSIC (Korean standard industrial classification) revised in 2008, a refinement of the sampling design for the survey becomes necessary, especially that for the business demand sector. This article, based on the 9th revision of the KSIC, constructs a stratification of the target population used for the e-learning industry status survey on the business demand sector. Classification of strata in the business population is based on the industrial type and employment scale of business. Under the stratified population, we design a sampling scheme by using the power allocation method that enables us to satisfy a target coefficient of variation of each industrial stratum. In order to secure an accurate survey results based on the proposed sampling design, we consider the problem of calculating the design weights, derivation of parameter estimators, and formulas of their standard errors.