• Applied Microscopy
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• v.46 no.2
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• pp.76-82
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• 2016

Aloe vera has been used in the pharmaceutical, food and cosmetic industry for its therapeutic properties. However, there are not many current studies on the microstructure of A. vera compared to studies on the chemical constituents and health efficacy of A. vera. Therefore, we compared the morphology of an A. vera leaf using an optical microscope, a conventional scanning electron microscope (SEM) and a cryo-SEM. Especially, this study focused on observing the gel in the inner leaf of A. vera, which is challenging using standard imaging techniques. We found that cryo-SEM is most suitable method for the observation of highly hydrated biomaterials such as A. vera without removing moisture in samples. In addition, we found the optimal analytical conditions of cryo-SEM. The sublimation conditions of $-100^{\circ}C$ and 10 minutes possibly enable the surface of the inner leaf of A. vera to be observed in their "near life-like" state with retaining moisture. The experiment was repeated with A. arborescens and A. saponaria to confirm the feasibility of the conditions. The results of this study can be applied towards the basic research of aloe and further extend previous knowledge about the surface structures of the various succulent plants.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.1
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• pp.17-22
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• 2004

A bacterial strain WJ-98 found to produce active extracellular keratinase was isolated from the soil of a poultry factory. It was identified as Paracoccus sp. based on its 16S rRNA sequence analysis, morphological and physiological characteristics. The optimal culture conditions for the production of keratinase by Paracoccus sp. WJ-98 were investigated. The optimal medium composition for keratinase production was determined to be 1.0% keratin, 0.05% urea and NaCl, 0.03% K$_2$HPO$_4$, 0.04% KH$_2$PO$_4$, and 0.01% MgCl$_2$$.$6H$_2$O. Optimal initial pH and temperature for the production of keratinase were 7.5 and 37$^{\circ}C$, respectively. The maximum keratinase production of 90 U/mL was reached after 84 h of cultivation under the optimal culturing conditions. The keratinase from Paracoccus sp. WJ-98 was partially purified from a culture broth by using ammonium sulfate precipitation, ion-exchange chromatography on DEAE-cellulose, followed by gel filtration chromatography on Sephadex G-75. Optimum pH and temperature for the enzyme reaction were pH 6.8 and 50$^{\circ}C$, respectively and the enzymes were stable in the pH range from 6.0 to 8.0 and below 50$^{\circ}C$. The enzyme activity was significantly inhibited by EDTA, Zn$\^$2+/ and Hg$\^$2+/. Inquiry into the characteristics of keratinase production from these bacteria may yield useful agricultural feed processing applications.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.8
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• pp.693-700
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• 2008

The primary mirror system in a satellite camera is an opto-mechanically coupled system for a reason that optical and mechanical behaviors are intricately interactive. In order to enhance the opto-mechanical performance of the primary mirror system, opto-mechanical behaviors should be thoroughly investigated by using various analysis procedures such as elastic, thermo-elastic, optical and eigenvalue analysis. In this paper, optimal design of the bipod flexure mounts for high opto-mechanical performance is performed. Optomechanical performances considered in this paper are RMS wavefront error under the gravity and thermal loading conditions and 1st natural frequency of the mirror system. The procedures of the flexure mounts design based on design of experiments and statistics is as follows. The experiments for opto-mechanical analysis are constructed based on the tables of orthogonal arrays and analysis of each experiment is carried out. In order to deal with the multiple opto-mechanical properties, MADM (Multiple-attribute decision making) is employed. From the analysis results, the critical design variables of the flexure mounts which have dominant influences on opto-mechanical performance are determined through analysis of variance and F-test. The regression model in terms of the critical design variables is constructed based on the response surfaceanalysis. Then the critical design variables are optimized from the regression model by using SQP algorithm. Opto-mechanical performance of the optimal bipod flexure mounts is verified through analysis.

• Korean journal of food and cookery science
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• v.26 no.1
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• pp.54-61
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• 2010

This study was performed to determine the optimal manufacturing conditions adding green laver, which has a rich aroma and unique taste, to Sulgidduk. The variables in green laver Sulgidduk production were amounts of green laver powder and water. Six sensory characteristics were used for sensory evaluations, including color, green laver flavor, green laver taste, moistness, softness, and elasticity. The optimal amounts of the powder and water were found to be 6.84 g for green laver powder and 31.08 g for water, satisfying a target sensory score (7.0/9.0) according to a response surface method. Sulgidduk with these optimal amounts of green laver and water was tasted by 118 consumers and showed a high acceptability score (6.94). Older consumers ($\geq$30 years old) preferred the color and flavor of green laver significantly more than younger consumers ($\leq$29 years old) did (p<0.01). Color analysis results of green laver Sulgidduk were significantly different in brightness, redness, and yellowness from those of Sulgidduk (control)(p<0.01). Texture analysis scores of green laver Sulgidduk were significantly lower than those of Sulgidduk (control) in hardness, adhesiveness, springiness, cohesiveness, gumminess, and chewiness, and they were significantly different in adhesiveness and chewiness (p<0.05).

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.9
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• pp.1152-1158
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• 2018

In this paper, Optimal operation plan through load analysis of industrial end-user is suggested. It calculated economic feasibility of ESS with detailed power lad analysis and conditions. Generally, if the latest maximum power is less than 30% of contracted power, it can not be peak shaving operation plan. and if the peak load level stays stead for 24 hours, it is difficult peak shaving for ESS. In addition to, When the peak load is occurred in summer or winter, a hybrid operation method combining the peak shaving plan and the time shift method is proposed. Therefore, When ESS is installed in industrial electrical customer, it is achieved best effect through the optimal operation plan.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.622-625
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• 2007

For optimizing the performance of SI engine such as engine torque, fuel consumption, and emissions, systems for variable valve timing were developed by many automotive researchers. In this work, we investigated the relationship between valve timing and intake orifice noise to improve the NVH (Noise, Vibration and Harshness) performance as well as engine torque and power. Two approaches are conducted, which are engine dynamometer testing and 1-D simulation analysis. Experimental data were measured on about 21 different operating conditions. This experiment shows that the intake and exhaust valve timing related to overlap period influence on the NVH performance, especially intake orifice noise of engine at given range of operation conditions. Similar results are achieved by using 1-D simulation analysis. It is concluded that the optimal strategies of controlling valve timing and tuning intake systems, are necessary to develop engines or vehicles with good sound quality.

• Korean Journal of Food Science and Technology
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• v.21 no.5
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• pp.655-661
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• 1989

Optimal drying conditions consisting of air temperature and relative humidity were searched by the simulation-optimization technique for minimizing quality changes in red pepper drying. Optimized drying conditions were analysed in the viewpoint of quality change kinetics and effects of control variables on the state variables. Optimal drying conditions were nearly same in both cases for carotenoid maximization and browning minimization. In two staged optimized drying, relative humidity took a lower search limit of about 10%, and air temperature in the first stage was near the lower limit of $50^{\circ}C$ and in second stage increased to a higher temperature varying with total drying time and stage changing time. Response surface analysis of time invariable drying confirmed the location of the optimal point lying on the vertex of lower limit humidity and a lowest drying temperature which ensures to attain target moisture of 0.2g water/g dry solid. Two stage drying can attain the higher objective function of quality by 3-5% than time invariable drying for shorter total drying times.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1612-1619
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• 2017

Objective: The objective of this study was to optimize ultrasonic-assisted enzymatic hydrolysis conditions, including enzyme-to-substrate (E/S) ratio, pH, and temperature, for producing porcine liver hydrolysates (PLHs) with the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity by using response surface methodology (RSM). Methods: The study used RSM to determine the combination of hydrolysis parameters that maximized the antioxidant activity of our PLHs. Temperature ($40^{\circ}C$, $54^{\circ}C$, and $68^{\circ}C$), pH (8.5, 9.5, and 10.5), and E/S ratio (0.1%, 2.1%, and 4.1%) were selected as the independent variables and analyzed according to the preliminary experiment results, whereas DPPH free radical scavenging activity was selected as the dependent variable. Results: Analysis of variance showed that E/S ratio, pH, and temperature significantly affected the hydrolysis process (p<0.01). The optimal conditions for producing PLHs with the highest scavenging activity were as follows: E/S ratio, 1.4% (v/w); temperature, $55.5^{\circ}C$; and initial pH, 10.15. Under these conditions, the degree of hydrolysis, DPPH free radical scavenging activity, ferrous ion chelating ability, and reducing power of PLHs were 24.12%, 79%, 98.18%, and 0.601 absorbance unit, respectively. The molecular weight of most PLHs produced under these optimal conditions was less than 5,400 Da and contained 45.7% hydrophobic amino acids. Conclusion: Ultrasonic-assisted enzymatic hydrolysis can be applied to obtain favorable antioxidant hydrolysates from porcine liver with potential applications in food products for preventing lipid oxidation.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.9
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• pp.1012-1019
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• 2012

In this study, CNG composite vessel is analyzed by using coupled model with liner and composite layer. For the coupled model, a method using theoretical analysis and FEA is suggested: elastic solution for laminated tube is used for theoretical analysis of the composite vessel, FEA is performed to the model of CNG composite vessel in actual conditions. On the basis of these results, optimal thickness and winding angle of the composite layer considering the material properties and thickness of the liner are determined. The results of theoretical analysis and FEA are compared with those carried out in previous studies for verifying the suggested analysis method.

• International Journal of Precision Engineering and Manufacturing
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• v.7 no.1
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• pp.18-23
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• 2006

Cutting by a high speed laser cutting machine is one of most effective technologies to improve productivity. This paper has presented the cutting characteristics and optimal cutting conditions in a high speed feeding type laser cutting machine by using Tacuchi method in the design of experiment. An L9(34) orthogonal array is adopted to study the effect of adjustment parameters. The adjustment parameters consist of cutting speed, laser power, laser output duty and assistant gas pressure. The surface roughness of sheet metal is regarded as a quality feature. Analysis of variance is performed in order to evaluate the effect of adjustment parameters on the quality feature of laser cutting process.