• Title/Summary/Keyword: conditions for optimal analysis

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Development and Application of the High Speed Weigh-in-motion for Overweight Enforcement (고속축하중측정시스템 개발과 과적단속시스템 적용방안 연구)

  • Kwon, Soon-Min;Suh, Young-Chan
    • International Journal of Highway Engineering
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    • v.11 no.4
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    • pp.69-78
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    • 2009
  • Korea has achieved significant economic growth with building the Gyeongbu Expressway. As the number of new road construction projects has decreased, it becomes more important to maintain optimal status of the current road networks. One of the best ways to accomplish it is weight enforcement as active control measure of traffic load. This study is to develop High-speed Weigh-in-motion System in order to enhance efficiency of weight enforcement, and to analyze patterns of overloaded trucks on highways through the system. Furthermore, it is to review possibilities of developing overweight control system with application of the HS-WIM system. The HS-WIM system developed by this study consists of two sets of an axle load sensor, a loop sensor and a wandering sensor on each lane. A wandering sensor detects whether a travelling vehicle is off the lane or not with the function of checking the location of tire imprint. The sensor of the WIM system has better function of classifying types of vehicles than other existing systems by detecting wheel distance and tire type such as single or dual tire. As a result, its measurement errors regarding 12 types of vehicle classification are very low, which is an advantage of the sensor. The verification tests of the system under all conditions showed that the mean measurement errors of axle weight and gross axle weight were within 15 percent and 7 percent respectively. According to the WIM rate standard of the COST-323, the WIM system of this study is ranked at B(10). It means the system is appropriate for the purpose of design, maintenance and valuation of road infrastructure. The WIM system in testing a 5-axle cargo truck, the most frequently overloaded vehicle among 12 types of vehicles, is ranked at A(5) which means the system is available to control overloaded vehicles. In this case, the measurement errors of axle load and gross axle load were within 8 percent and 5 percent respectively. Weight analysis of all types of vehicles on highways showed that the most frequently overloaded vehicles were type 5, 6, 7 and 12 among 12 vehicle types. As a result, it is necessary to use more effective overweight enforcement system for vehicles which are seriously overloaded due to their lift axles. Traffic volume data depending upon vehicle types is basic information for road design and construction, maintenance, analysis of traffic flow, road policies as well as research.

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Changes in the Hyperspectral Characteristics of Wheat Plants According to N Top-dressing Rates at Various Growth Stages (밀에서 질소 시비 조건에 따른 생육 단계별 초분광 특성 변화)

  • Jung, Jae Gyeong;Lee, Yeong Hun;Choi, Jae Eun;Song, Gi Eun;Ko, Jong Han;Lee, Kyung Do;Shim, Sang In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.377-385
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    • 2020
  • Recently, wheat consumption has been increasing in Korea, requiring increased production. Nitrogen fertilization is a critical determinant in crop yield; therefore, it is necessary to optimize the nitrogen fertilization regime with current trends that emphasize the minimum impact of nitrogen fertilizer on the environment. In this study, both nondestructive spectral analysis using a hyperspectral camera and growth analysis were performed to determine the optimal N top-dressing rates after heading. The nitrogen application regimes consisted of three conditions according to the secondary top-dressing rate: N4:3:0 (0 kg 10 a-1), N4:3:3 (2.73 kg 10 a-1), and N4:3:6 (5.46 kg 10 a-1). Subsequently, growth and physiological investigations were performed at the jointing, heading, and ripening stages of wheat, and spectral investigations were conducted. On April 29, as the nitrogen fertilization rate was increased to N4:3:3 and N4:3:6, plant height and grain yield increased by 4% and 8%, and 8% and 52%, respectively, compared to those under N4:3:0. Leaf area index and SPAD value also increased by 13% and 24%, and 32% and 43%, respectively. The R (red), G (green), and B (blue) of leaf color were lowered by 15, 11, and 4 in N4:3:3 and 44, 34, and 18 in N4:3:6, respectively, as compared to the control. Grain yield was the highest at high top-dressing (N4:3:6), however, there was no difference between no top-dressing (N4:3:0) and intermediat top-dressing (N4:3:3). The reflectance analyzed using a hyperspectral camera showed a difference in the near-infrared (NIR) region on March 19, and on April 29, there was a difference both in the visible light region greater than 550 nm and the NIR region. Vegetation indices differed according to fertilization regime, except for the greenness index (GI). The results of this study showed that not only growth and physiological analysis but also spectral indices can be used to optimize the nitrogen top-dressing rate.

Effect of the Degree of Weathering on the Distribution of Aggregate Particle Size and the Generation of Fine Rock Particles during Crushing of Granite (화강암 파쇄시 풍화정도가 골재 입도분포 및 미석분 발생에 미치는 영향)

  • You, Byoung-Woon;Lee, Jin-Young;Lee, Dong-kil;Cheong, Young-Wook
    • Economic and Environmental Geology
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    • v.55 no.5
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    • pp.429-438
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    • 2022
  • This study evaluated the effect of the degree of weathering on the particle size distribution and the amount of fine particles generated in the aggregate production process during the crushing of igneous rock. Rock samples were collected from three areas with differences in strength from the Schmith hammer measurement at the aggregate quarry in Geochang, Gyeongsangbuk-do. After crushing with a jaw crusher under the same conditions in laboratory, particle size analysis, mineral analysis, chemical analysis, and weathering index were calculated. The Schmidt hammer measurements were 56, 28, and <10, and the CIA and CIW values of weathering index were also different, so the rock samples were classified into hard rock, soft rock, and weathered rock according to the weathering degree. It shows a smaller particle size distribution toward weathered rocks under the microscope, and the proportion of altered clay minerals such as sericite increased. The composition of feldspar and quartz was high for hard rock, and the ratio of muscovite and kaolinite was low. As a result of the crushing of the jaw crusher, hard rock produced a lot of coarse crushed material (13.2mm), while soft rock and weathered rock produced fine crushed material (4.75mm). The former showed the characteristics of the beta distribution curve, and the latter showed the bimodal distribution curve. The production of fine rock particles (based on 0.71mm of sieve, wt. %) increased to 13%<21%<22% in hard rock, soft rock, and weathered rock, and the greater the degree of weathering, the more fine rock particles were generated. The fine particles are recovered by the operation of the sand unit in the wet aggregate production process. Therefore, in order to minimize the amount of sludge generated in the aggregate production process, it was judged that a study on the optimal operation of cyclones could be necessary.

Optimization of Human Thrombopoietin Production in Insert Cells Using Baculovirus Expression System (베큘로 바이러스 발현 시스템에 의한 곤충세포에서의 인간 트롬보포이에틴 생산 최적화)

  • 고여욱;손미영;박상규;안혜경;박승국;박명환;양재명
    • KSBB Journal
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    • v.13 no.2
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    • pp.181-186
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    • 1998
  • In order to obtain high-level production of recombinant human thrombopoietin (rhTPO) in insect cell line, HTI-TN-5B1-4 (TN5), conditions for optimal rhTPO expression such as multiplicity of infection (MOI), the cell density at infection, harvesting time and type of culture method as well as growth media were determined. When TN5 cells were cultured as anchorage-dependent state in 60-mm dish, cell density $2\times^6$ cells,MOI of 10 and Garvesting the culture media at 72 hr post-infection wrere the cinditions for highest rh TPO production. High production of rhTPO was also achieved by using EXPRESS FIVE serum free media rather than SF900II serum free media-1. Anchorage-dependent TN5 cells were adapted as a suspension culture when they were grown in the presence of heparin. TN5 cells were successfully cultured at 0.2 L scale in suspension culture without having aggregation. When TN5 cells were cultured as suspension state, cell density of $0.6\times10^6$ cells/mL, MOI of 1 and harvesting the culture media at 72 hr post-infection, gave the highest yield of rhTPO.

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A study on the Degradation and By-products Formation of NDMA by the Photolysis with UV: Setup of Reaction Models and Assessment of Decomposition Characteristics by the Statistical Design of Experiment (DOE) based on the Box-Behnken Technique (UV 공정을 이용한 N-Nitrosodimethylamine (NDMA) 광분해 및 부산물 생성에 관한 연구: 박스-벤켄법 실험계획법을 이용한 통계학적 분해특성평가 및 반응모델 수립)

  • Chang, Soon-Woong;Lee, Si-Jin;Cho, Il-Hyoung
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.1
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    • pp.33-46
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    • 2010
  • We investigated and estimated at the characteristics of decomposition and by-products of N-Nitrosodimethylamine (NDMA) using a design of experiment (DOE) based on the Box-Behken design in an UV process, and also the main factors (variables) with UV intensity($X_2$) (range: $1.5{\sim}4.5\;mW/cm^2$), NDMA concentration ($X_2$) (range: 100~300 uM) and pH ($X_2$) (rang: 3~9) which consisted of 3 levels in each factor and 4 responses ($Y_1$ (% of NDMA removal), $Y_2$ (dimethylamine (DMA) reformation (uM)), $Y_3$ (dimethylformamide (DMF) reformation (uM), $Y_4$ ($NO_2$-N reformation (uM)) were set up to estimate the prediction model and the optimization conditions. The results of prediction model and optimization point using the canonical analysis in order to obtain the optimal operation conditions were $Y_1$ [% of NDMA removal] = $117+21X_1-0.3X_2-17.2X_3+{2.43X_1}^2+{0.001X_2}^2+{3.2X_3}^2-0.08X_1X_2-1.6X_1X_3-0.05X_2X_3$ ($R^2$= 96%, Adjusted $R^2$ = 88%) and 99.3% ($X_1:\;4.5\;mW/cm^2$, $X_2:\;190\;uM$, $X_3:\;3.2$), $Y_2$ [DMA conc] = $-101+18.5X_1+0.4X_2+21X_3-{3.3X_1}^2-{0.01X_2}^2-{1.5X_3}^2-0.01X_1X_2+0.07X_1X_3-0.01X_2X_3$ ($R^2$= 99.4%, 수정 $R^2$ = 95.7%) and 35.2 uM ($X_1$: 3 $mW/cm^2$, $X_2$: 220 uM, $X_3$: 6.3), $Y_3$ [DMF conc] = $-6.2+0.2X_1+0.02X_2+2X_3-0.26X_1^2-0.01X_2^2-0.2X_3^2-0.004X_1X_2+0.1X_1X_3-0.02X_2X_3$ ($R^2$= 98%, Adjusted $R^2$ = 94.4%) and 3.7 uM ($X_1:\;4.5\;$mW/cm^2$, $X_2:\;290\;uM$, $X_3:\;6.2$) and $Y_4$ [$NO_2$-N conc] = $-25+12.2X_1+0.15X_2+7.8X_3+{1.1X_1}^2+{0.001X_2}^2-{0.34X_3}^2+0.01X_1X_2+0.08X_1X_3-3.4X_2X_3$ ($R^2$= 98.5%, Adjusted $R^2$ = 95.7%) and 74.5 uM ($X_1:\;4.5\;mW/cm^2$, $X_2:\;220\;uM$, $X_3:\;3.1$). This study has demonstrated that the response surface methodology and the Box-Behnken statistical experiment design can provide statistically reliable results for decomposition and by-products of NDMA by the UV photolysis and also for determination of optimum conditions. Predictions obtained from the response functions were in good agreement with the experimental results indicating the reliability of the methodology used.

Quantitative Analysis of Carbohydrate, Protein, and Oil Contents of Korean Foods Using Near-Infrared Reflectance Spectroscopy (근적외 분광분석법을 이용한 국내 유통 식품 함유 탄수화물, 단백질 및 지방의 정량 분석)

  • Song, Lee-Seul;Kim, Young-Hak;Kim, Gi-Ppeum;Ahn, Kyung-Geun;Hwang, Young-Sun;Kang, In-Kyu;Yoon, Sung-Won;Lee, Junsoo;Shin, Ki-Yong;Lee, Woo-Young;Cho, Young Sook;Choung, Myoung-Gun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.3
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    • pp.425-430
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    • 2014
  • Foods contain various nutrients such as carbohydrates, protein, oil, vitamins, and minerals. Among them, carbohydrates, protein, and oil are the main constituents of foods. Usually, these constituents are analyzed by the Kjeldahl and Soxhlet method and so on. However, these analytical methods are complex, costly, and time-consuming. Thus, this study aimed to rapidly and effectively analyze carbohydrate, protein, and oil contents with near-infrared reflectance spectroscopy (NIRS). A total of 517 food samples were measured within the wavelength range of 400 to 2,500 nm. Exactly 412 food calibration samples and 162 validation samples were used for NIRS equation development and validation, respectively. In the NIRS equation of carbohydrates, the most accurate equation was obtained under 1, 4, 5, 1 (1st derivative, 4 nm gap, 5 points smoothing, and 1 point second smoothing) math treatment conditions using the weighted MSC (multiplicative scatter correction) scatter correction method with MPLS (modified partial least square) regression. In the case of protein and oil, the best equation were obtained under 2, 5, 5, 3 and 1, 1, 1, 1 conditions, respectively, using standard MSC and standard normal variate only scatter correction methods with MPLS regression. Calibrations of these NIRS equations showed a very high coefficient of determination in calibration ($R^2$: carbohydrates, 0.971; protein, 0.974; oil, 0.937) and low standard error of calibration (carbohydrates, 4.066; protein, 1.080; oil, 1.890). Optimal equation conditions were applied to a validation set of 162 samples. Validation results of these NIRS equations showed a very high coefficient of determination in prediction ($r^2$: carbohydrates, 0.987; protein, 0.970; oil, 0.947) and low standard error of prediction (carbohydrates, 2.515; protein, 1.144; oil, 1.370). Therefore, these NIRS equations can be applicable for determination of carbohydrates, proteins, and oil contents in various foods.

Analysis of Meteorological Elements in the Cultivated Area of Hadong Green Tea (하동녹차 재배지역의 기상요소별 분석)

  • Hwang, Jung-Gyu;Kim, Jong-Cheol;Cho, Kyoung-Hwan;Han, Jae-Yoon;Kim, Ru-Mi;Kim, Yeon-Su;Cheong, Gang-Won;Kim, Yong-Duck
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.12 no.2
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    • pp.132-142
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    • 2010
  • Characteristics of meteorological elements were analyzed at Hwagae and Agyang where are the representative areas of Hadong green tea cultivation in Korea. An automatic weather monitoring system (AWS) and a simple data log were employed to measure meteorological data such as temperature, relative humidity, precipitation, and wind direction and speed for 2009. The annual average air temperature of Hwagae and Agyang was 14.5 and 14.2, respectively, showing the warmest month in August ($25.4^{\circ}C$ for Hwagae and $24.9^{\circ}C$ for Agyang) and the coldest month in January ($0.3^{\circ}C$ for Hwagae and $0.2^{\circ}C$ for Agyang). Annual average of daily temperature difference (= daily maximum temperature - daily minimum temperature) was $11.3^{\circ}C$ for Hwagae and $11.1^{\circ}C$ for Agyang. Hwagae and Agyang had 62.7% and 65.3% of the annual average relative humidity, respectively. Annual precipitation was 1387 mm for Hwagae and 1793 mm for Agyang of which were higher of 605mm for Hwagae and 835 mm for Agyang compared to that in 2008. Majority of precipitation occurred between May and August, attributing 77.6% for Hwagae and 76.6% for Agyang to the annual precipitation. The annual total sunshine duration was 2054.3 hrs in Hwagae with the longest monthly sunshine duration in May (235.1 hrs) and the shortest monthly sunshine duration in July (102.5 hrs). Dominant wind direction changed seasonally from northwesterly wind in fall and winter to southeasterly wind in spring and summer. The annual average wind speed was 1.5 m $s^{-1}$ with the highest monthly wind speed of 2.0 m $s^{-1}$ in December and the lowest monthly wind speed of 1.1 m $s^{-1}$ in February. It is expected that continuous observation and assessment of meteorological data will improve our understanding of optimal environmental conditions for green tea cultivation and be used for developing models of green tea cultivation in the Hadong area.

Effects of Different Nitrogen Levels and Planting Densities on the Quality and Quantity of 'Nunkeunheugchal' Rice (시비량과 재식밀도 변화에 따른 '눈큰흑찰'의 품질 및 수량변화)

  • Bae, Hyun Kyung;Oh, Seong Hwan;Seo, Jong Ho;Hwang, Jung Dong;Kim, Sang Yeol;Oh, Myung Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.2
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    • pp.118-123
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    • 2017
  • 'Nunkeunheugchal' is a waxy black rice variety that has a large embryo. The quality of black rice depends on the anthocyanin content of the rice seed coat, which is mainly determined by cultivation environment. Factors that affect the anthocyanin content include nitrogen level, planting density, transplanting date and harvesting date. This study was carried out to investigate the optimum black rice cultivation conditions by examining the effects of different nitrogen levels and planting densities. An initial study was conducted to determine the optimum nitrogen level in which four levels of nitrogen were applied to the field (0, 4, 8 and 12 kg/10a). As the nitrogen contents were increased up to 8 kg/10a, there was a concomitant increase in rice yields. However, nitrogen levels greater than 8 kg/10a, the yield was maintained at the same level. Correlation analysis indicated that the optimum nitrogen level for maximum yield was 9.6 kg/10a. In addition, anthocyanin levels showed a trend similar to that of yield, with correlation analysis indicating that the optimum nitrogen level for maximum anthocyanin content is 10.6 kg/10a.On the basis of these results, a second study was conducted to determine the optimum combination of planting density and nitrogen level. The planting densities investigated were $30{\times}12$, $30{\times}14$, $30{\times}16$ and nitrogen levels were 7, 9 and 12 kg/10a. A high planting density ($30{\times}12cm$) was shown to produce higher numbers of tillers and yield. As calculated in the first study, a nitrogen level of 9 kg/10a shown to produce the highest anthocyanin content and yield. Collectively, the results of this study indicate that a planting density of $30{\times}12cm$ and a nitrogen level of 9 kg/10a is the optimal combination in terms of maximizing both rice yield and anthocyanin content.

Optimizing In Vitro Propagation of Sophora koreensis Nakai using Statistical Analysis (다양한 통계분석 기법을 이용한 개느삼(Sophora koreensis Nakai)의 기내 증식 최적 조건 구명)

  • Jeong, Ukhan;Lee, Hwa;Park, Sanghee;Cheong, Eun Ju
    • Journal of Korean Society of Forest Science
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    • v.110 no.1
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    • pp.53-63
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    • 2021
  • Sophora koreensis Nakai is an indigenous plant in Koreawith a restricted natural range, part of which is in Gangwon province. The species is known to contain phytochemicals that have beneficial effects on human health, and it is economically important in bioindustry. Because of the limited number of plants in a small range of habitats, the mass-propagation method should be developed for use and conservation. In vitro tissue culture is a reliable method in terms of mass propagation from selected clones of the species. We investigated the optimal conditions of the medium in this process, especially focusing on the concentrations of plant growth regulators(PGRs) in the culture of stem-containing axillary buds. Three statistical methods, i.e., ANOVA, response surface method(RSM), and fuzzy clustering were used to analyze the plant growth, number of shoots induced, and shoot length with various combinations of PGRs. Results from the RSM differed from those of the other two methods; thus, the method was not suitable. ANOVA and fuzzy clustering showed similar results. However, more accurate results were obtained using fuzzy clustering because it provided a probability for each treatment. On the basis of the fuzzy clustering analysis, stem tissue produced the greatest number of shoots(11.03 per explant; 63.33%) on a medium supplemented with 5-��M 6-benzylaminopurine and 2.5-��M thidiazuron(TDZ). Proliferation of shoots(2.18 ± 0.21 cm, 63.33%) was attained on a medium supplemented with 2.5-��M BA, 2.5-��M TDZ, and 2.5-��M gibberellic acid.

Preparation of Pure CO2 Standard Gas from Calcium Carbonate for Stable Isotope Analysis (탄산칼슘을 이용한 이산화탄소 안정동위원소 표준시료 제작에 대한 연구)

  • Park, Mi-Kyung;Park, Sunyoung;Kang, Dong-Jin;Li, Shanlan;Kim, Jae-Yeon;Jo, Chun Ok;Kim, Jooil;Kim, Kyung-Ryul
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.18 no.1
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    • pp.40-46
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    • 2013
  • The isotope ratios of $^{13}C/^{12}C$ and $^{18}O/^{16}O$ for a sample in a mass spectrometer are measured relative to those of a pure $CO_2$ reference gas (i.e., laboratory working standard). Thus, the calibration of a laboratory working standard gas to the international isotope scales (Pee Dee Belemnite (PDB) for ${\delta}^{13}C$ and Vienna Standard Mean Ocean Water (V-SMOW) for ${\delta}^{18}O$) is essential for comparisons between data sets obtained by other groups on other mass spectrometers. However, one often finds difficulties in getting well-calibrated standard gases, because of their production time and high price. Additional difficulty is that fractionation processes can occur inside the gas cylinder most likely due to pressure drop in long-term use. Therefore, studies on laboratory production of pure $CO_2$ isotope standard gas from stable solid calcium carbonate standard materials, have been performed. For this study, we propose a method to extract pure $CO_2$ gas without isotope fractionation from a solid calcium carbonate material. The method is similar to that suggested by Coplen et al., (1983), but is better optimized particularly to make a large amount of pure $CO_2$ gas from calcium carbonate material. The $CaCO_3$ releases $CO_2$ in reaction with 100% pure phosphoric acid at $25^{\circ}C$ in a custom designed, evacuated reaction vessel. Here we introduce optimal procedure, reaction conditions, and samples/reactants size for calcium carbonate-phosphoric acid reaction and also provide the details for extracting, purifying and collecting $CO_2$ gas out of the reaction vessel. The measurements for ${\delta}^{18}O$ and ${\delta}^{13}C$ of $CO_2$ were performed at Seoul National University using a stable isotope ratio mass spectrometer (VG Isotech, SIRA Series II) operated in dual-inlet mode. The entire analysis precisions for ${\delta}^{18}O$ and ${\delta}^{13}C$ were evaluated based on the standard deviations of multiple measurements on 15 separate samples of purified $CO_2$. The pure $CO_2$ samples were taken from 100-mg aliquots of a solid calcium carbonate (Solenhofen-ori $CaCO_3$) during 8-day experimental period. The multiple measurements yielded the $1{\sigma}$ precisions of ${\pm}0.01$‰ for ${\delta}^{13}C$ and ${\pm}0.05$‰ for ${\delta}^{18}O$, comparable to the internal instrumental precisions of SIRA. Therefore, we conclude the method proposed in this study can serve as a way to produce an accurate secondary and/or laboratory $CO_2$ standard gas. We hope this study helps resolve difficulties in placing a laboratory working standard onto the international isotope scales and does make accurate comparisons with other data sets from other groups.