• 한국환경보건학회지
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• 제31권6호
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• pp.457-466
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• 2005

Industrial plasticizers such as phthalates can induce peroxisome proliferation. A growing concern among environmental and health groups has arisen because phthalates such as di-2-ethy1hexy1 phthalate (DEHP) and DBP may cause hormonal disorders, reproductive toxicity, hepatocellular tumors, genital disorders owing to a capacity to bind estrogen receptors, and a low-dose toxic action during certain periods of fetal development. Phthalate esters are used extensively as a plasticizer for plastic manufacture such as PVC bags and medical devices. This study investigated the effects of leached components from spring water container's cap and seal film. Phthalates, e.g. dimethy1 phthalate (DMP), diethy1 phthalate (DEP), di-n-buty1 phthalate (DBP), benzy1buty1 phthalate (BBP), di-(2-ethy1hexy1) phthalate (DEHP), and bisphenol A (BPA) were measured in the spring water. The bisphenol A was not detected or below the detection limit on the leaching from cap, sealing or spring water. DEHP were detected 90-116 ppb on the leaching from seal after 2 weeks, and 0.48-0.51 ppb from the spring water after I week. BBP were measured from seal within 1 week 25.4-66 ppb and below 0.12 ppb from spring water within 2 days. DMP were detected from seal within 2 weeks 51-68.5 ppb and 0.12 ppb after 2 weeks. DEP were measured from seal within 2 weeks 48.1-141 ppb and the concentrations were increased by the time from 0.10 to 0.31 ppb at spring water. DBP were detected from the seal within 2 weeks 92.3-5100 ppb and the concentration were decreased by the time from 0.24 to 0.10 ppb at spring water. These results indicate that some phthalate esters contaminated with spring water using the intact cap and seal film. It is concluded that the measured levels of phthalates leaching from these materials might in vivo only be slightly less than 1/10 of the LOAEL.

• 한국산림과학회지
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• 제106권2호
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• pp.111-120
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• 2017

IUCN의 적색목록은 전 세계 규모에서 가장 신뢰받는 멸종, 희귀 동식물종의 평가와 보전 정보를 제공하고 있다. 2016년 7월까지 한반도에 분포하는 식물중 IUCN 적색목록 평가를 받은 종은 236종으로, 5종이 멸종위기종(CR), 20종이 위기종(EN), 9종이 취약종(VU)으로 확인되었다. 나머지 189종은 약관심종(LC)으로 평가되었다. 환경부는 2011년 국가적 색목록 평가라는 명목 하에 IUCN 적색목록과 내용, 형식이 다른 정성적 기준에 따라 평가 결과를 제시하였다. 이와는 별개로 환경부는 멸종위기종 I급과 II급 목록을 57종으로 수정 제시하였다. 이 목록은 IUCN의 적색목록과 비교시 일부 공통종이 있으나 공통종도 보전상의 지위가 일치하지 않는다. 이런 평가 내용의 불일치의 이유는 남한이라는 제한된 지역 수준의 평가로는 전 세계 규모의 평가와는 결과가 일치할 수 없기 때문이다. 평가에 필요한 정보가 부실하게 제시되거나 정보가 없는 상태에서 자의적 평가가 이루어진 경우도 있다. 국가 단위의 종 관리를 위해서는 멸종과 절멸에 대한 엄밀한 용어 적용과 남한에 국한된 제한적 시각의 편향된 결과를 배제하고, 분류학적 실체에 근거한 종 선정, 평가를 위한 개체군의 크기, 분포, 개체수, 개체군 증감에 대한 구체적 자료 제시와 수집이 필요하다. 환경부의 현재 멸종위기동식물 목록은 '국내절멸위기목록'으로 변경하고, 전 세계 단위의 IUCN적색목록 평가종을 '멸종위기목록'으로 이원화해서 관리할 필요가 있다. 현재 국가 기관에서 제시하는 몇 백 종의 과도한 목록 보다는 우선 평가 대상의 종을 순차적으로 늘려가면서, 평가 및 종관리에 내실을 다지는 것이 필요하다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3437-3445
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• 2016

Background: There is an increasing concern in the role of microRNA (miRNA) in the pathogenesis of bone metastasis (BM) secondary to prostate cancer (CaP). In this exploratory study, we hypothesized that the expression of vinculin (VCL) and chemokine X3C ligand 1 (CX3CL1) might be down-regulated in clinical samples, most likely due to the post-transcriptional modification by microRNAs. Targeted genes would be up-regulated upon transfection of the bone metastatic prostate cancer cell line, PC3, with specific microRNA inhibitors. Materials and Methods: MicroRNA software predicted that miR-21 targets VCL while miR-29a targets CX3CL1. Twenty benign prostatic hyperplasia (BPH) and 16 high grade CaP formalin-fixed paraffin embedded (FFPE) specimens were analysed. From the bone scan results, high grade CaP samples were further classified into CaP with no BM and CaP with BM. Transient transfection with respective microRNA inhibitors was done in both RWPE-1 (normal) and PC3 cell lines. QPCR was performed in all FFPE samples and transfected cell lines to measure VCL and CX3CL1 levels. Results: QPCR confirmed that VCL messenger RNA (mRNA) was significantly down-regulated while CX3CL1 was up-regulated in all FFPE specimens. Transient transfection with microRNA inhibitors in PC3 cells followed by qPCR of the targeted genes showed that VCL mRNA was significantly upregulated while CX3CL1 mRNA was significantly down-regulated compared to the RWPE-1 case. Conclusions: The down-regulation of VCL in FFPE specimens is most likely regulated by miR-21 based on the in vitro evidence but the exact mechanism of how miR-21 can regulate VCL is unclear. Up-regulated in CaP, CX3CL1 was found not regulated by miR-29a. More microRNA screening is required to understand the regulation of this chemokine in CaP with bone metastasis. Understanding miRNA-mRNA interactions may provide additional knowledge for individualized study of cancers.

• 한국콘텐츠학회논문지
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• 제13권11호
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• pp.988-1003
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• 2013

본 연구는 예비유아교사들의 영유아권리의 중요성 및 권리교육에 대한 필요성의 인식을 알아보기 위한 목적으로 서울, 경기, 충청, 전라, 제주도의 3년제 전문대학 6곳의 유아교육과 1, 2, 3학년 525명을 대상으로 설문조사하였다. 그 결과, 첫째, 예비유아교사들은 영유아 권리의 중요성을 비교적 높게 인식하고 있었다. 예비유아교사들은 학년이 높을수록 영유아의 권리를 더 중요하다고 인식하였다. 또한 권리관련 교과목을 수강한 예비유아교사들이 영유아권리가 더 중요하다고 인식하였다. 둘째, 예비유아교사들은 영유아의 권리교육 필요성을 높게 인식하고 있었다. 1, 2학년보다 3학년이 영유아의 권리교육이 더 필요하다고 인식하였다. 반면 권리관련 과목 수강여부에 따라서는 영유아 권리교육의 필요성에 대한 인식은 차이가 없었다. 셋째, 예비유아교사들은 영유아 권리의 중요성 보다 권리교육의 필요성을 높게 인식하고 있는 것으로 나타났다.

• Journal of Nutrition and Health
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• 제9권1호
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• pp.16-24
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• 1976

To determine patterns of preference for and consumption of milk and milk products in Seoul city, a study was conducted of 724 girls and 587 boys ranging in age from elementary school to collage level, from July to August, 1974. Findings are summarized as follows: In general, milk and milk products were liked by both children and adults. A contrasting low tendency to purchase such products was due to socioeconomics, habitual, and educational factors, as well as a lack of adequate nutritional information, especially with regard to milk. Knowledge of the value of milk as a source of calcium was lacking, especially among boys. The majority of homemakers understood that milk was nutritious, but not specifically as the best source of calcium for their families. Milk, especially in beverage form, was commonly regarded as food for the infant and small child. Grandparents would not drink it. Such a beliefe is similar to that found in under-developed or developing nations. The need for nutrition education must be emphasized. Milk and milk products are expensive foods, particularly for the low income family; but even in higher income levels, consumption was limited. Milk was consumed mostly by the younger family members, less by the older. Among the children, preference, knowledge, and concern with the nutritional value of milk increased with age of subjects. It is presumed that awareness improved with increasing length of school education. Greater frequency of milk intolerance was found with decreasing age of respondents: about 10 percent had some form of milk intolerance. This percentage will be found to be much higher in villages where milk is less familiar. This study indicated that respondents liked ice cream best of all the milk products. For beverage, they preferred cider and cola in summer, coffee and milk in winter. Smaller families consumed more milk than larger families. Household consumption of condensed milk was very low, mostly as coffeemate. Among households interviewed, as many had regular as irregular delivery of dairy milk. Average consumption ranged 0.5 to 1.5 Hop. Engel indices of these families ranged 20 to 60. Use of milk at home was very simple. Not many methods of preparation were found. The milk was used mostly as beverage. In order to improve the nutritional level of Korean children and adults who especially need additional calcium, milk and milk products are recommended as the best source of calcium. Nutrition education of children and their parents should receive greater emphasis.

• 부모자녀건강학회지
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• 제4권1호
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• pp.35-55
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• 2001

Recently attention has been focused on the effects of early intervention, or its lack, on both normal and preterm infants. Particularly numerous studies suggest that premature infants are not necessarily understimulated but instead are subjected to inappropriate stimulation. Developmental support and sensory stimulation have become clinical opportunities in which nursing practice can impact on the neurobehavioral outcome of premature infants. Developmental care has been widely accepted and implemented in neonatal intensive care units across the country. Increasingly, attention and concern in caring for low-birth-weight infants and premature infants has led clinicians in the field to explore the effects of a complex of interventions designed to create and maintain a developmentally supportive environment; to provide age-appropriate sensory input; and to protect the infant from inappropriate, excessive and stressful stimulation. The components of developmental care include modifications of the macro-environment to reduce NICU light and sound levels, care clustering, nonnutritive sucking, and containment strategies, such as flexed positioning or swaddling. Sensory stimulation of the premature infants is presented to standardize the modification of a developmental intervention based on physiologic and behavioral cues. The most appropriate type of stimuli are those that are sensitive to infant cues. Evaluation of infant physiological and behavioral responds to specific intervention stimuli may help to identify more appropriate interventions based on infants' cues. A critical question confronting the clinician is that of determining when the evidence supporting a change in practice is sufficient to justify making that change. There are acknowledged limitations in the current studies. Many of the studies examined had small sample sizes; used nonprobability sampling; and used a phase lag design, which introduces the possibility of threats to internal validity and limits the generalizability of the results. Although many issues regarding the effects of developmental interventions remain unresolved, the available research base documents significant benefits of developmental care for LBW infants in consistent outcomes, without significant adverse effects. Particularly, although the individual studies vary somewhat in the definition of specific outcomes measured, instrumentation used, time and method of data collection, and preparaion of the care providers, in all studies, infants receiving the full protocol of individualized developmentally supportive care had improvements in some aspect of four areas of infant functioning: level of respiratory or oxygen support, the establishment of oral feeding; length of hospital stay, and infant behavioral regulation. In summary, based on the available literature, individualized developmental intervention should be incorporated into standard practice in neonatal intensive care. And this implementation needs to be coupled with ongoing research to evaluate the impact of an individualized developmental care programs on the short- and long-tenn health outcomes of LBW infants.

• Journal of Nutrition and Health
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• 제49권4호
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• pp.233-240
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• 2016

항산화, 항암 및 항염증 효능 등의 생리 활성이 보고된 우엉 뿌리의 열수 추출물을 제조하여 advanced glycation end products (AGEs) 형성 저해 효과를 확인하였다. AGEs는 bovine serum albumin (BSA)과 단당류인 glucose, fructose, galactose를 혼합하여 $37^{\circ}C$에서 3주간 배양하여 생성하였고 매주 형광도 측정, fructosamine 분석, ${\alpha}$-dicarbonyl compounds 함량 분석을 통해 AGEs의 생성량을 확인하였다. 우엉 뿌리 추출물의 AGEs 생성 저해능을 AGEs 생성 억제제로 알려져 있는 aminoguanidine (AG)의 저해능과 비교하였다. 우엉 뿌리 추출물은 BSA와 단당류인 glucose, fructose, galactose 각각의 당화반응을 억제하였으며 특히 배양 3주차에서 BSA와 glucose의 당화반응 결과물인 AGEs 생성을 유의적으로 저해하였다. 농도 2 mg/mL 이상의 우엉 뿌리 추출물은 1 mM AG보다 AGEs 생성 저해능이 우수하였으며 농도 4 mg/mL의 우엉 뿌리 추출물은 배양 3주차에서 AGEs 생성을 약 80% 이상 억제하는 효능을 나타냈다. 체내 혈당은 당뇨병과 같은 질환과 식이의 영향을 받으므로 다양한 glucose 농도에서 우엉 뿌리 추출물의 AGEs 생성 억제능을 조사하였다. 그 결과 AGEs 생성은 glucose의 농도에 비례하여 증가하였으며 우엉 뿌리 추출물은 당뇨환자의 식후에 관찰되는 혈당인 25 mM glucose 군에서 1 mM의 AG보다 높은 우수한 저해 효과를 확인하였다. 본 연구 결과는 우엉 뿌리 추출물의 AGEs 생성 억제라는 새로운 기능성을 밝히며 향후 당뇨 합병증 예방 효능을 가진 기능성 식품으로의 개발 가능성을 제시한다.

• 대한토목학회논문집
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• 제34권3호
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• pp.989-998
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• 2014

최근 아이슬란드의 에야프얄라요쿨화산과 인도네시아의 메라피화산의 등 대형화산재해에 따른 국가적 손실이 세계 곳곳에서 빈번하게 발생하고 있으며 천문학적인 경제적 손실과 인명손실이 발생하였다. 국내의 경우 한반도의 백두산이 최근 빈번한 지진파가 발생하는 가운데 백두산화산 분화의 가능성이 고조되고 있는 현실이다. 본 논문은 사회기반시설에 대해 18가지로 분류를 통해 인도네시아 현지 공무원, 주민, 연구원을 대상으로 사회기반시설의 복구우선순위에 대한 리커트 5점척도 방법으로설문을 실시하여 설문결과에 가중치를 적용, 정량적인 복구우선순위 결과와 사회기반시설의 복구 니즈를 도출하였다. 국내의 경우 화산재해의 피해경험이 전무하기 때문에 이에 따른 피해자들의 복구수요파악이 부족한 실정이다. 따라서 본 연구를 통해 도출된 재해 복구 우선순위를 국내 대형 화산재해 발생 시 복구 우선순위로 활용할 수 있을 것으로 기대하며 연구결과를 활용하여 화산재해 발생 시 복구, 복원 시 참고할 수 있는 자료로써 활용될 수 있을 것으로 기대한다.

• 한국환경농학회지
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• 제35권1호
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• pp.55-61
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• 2016

다년생 근채류인 인삼, 산양삼, 더덕, 도라지의 중금속 함량 실태조사를 통해 우리나라 국민의 식품 섭취로 인한 중금속 노출수준에 따른 위해성을 평가하였으며, 이를 위해 총 214건을 수거하였다. 근채류 중 납, 카드뮴 및 비소 함량분석을 위해 microwave장치를 이용해 전처리한 후 ICP/MS로 측정하는 시험법을 확립하였다. 중금속의 검출한계는 0.010~0.050 μg/kg이었으며 정량한계는 0.035~0.175 μg/kg이었다. 표준용액 첨가법과 CRM을 이용하여 회수율을 실험한 결과, 76~102%의 양호한 결과를 얻었다. 다년생 근채류 중 납 함량은 평균 0.013(인삼)~0.070(산양삼) mg/kg이었고, 카드뮴 함량은 평균 0.009(인삼)~0.034(더덕) mg/kg이었으며 비소 함량은 평균 0.002(인삼)~0.004(도라지) mg/kg이었다. 다년생 근채류 섭취에 따른 중금속 위해성 평가를 수행한 결과, 중금속 노출량은 각각 Pb 0.070 μg/day, Cd 0.041 μg/day, As 0.008 μg/day으로 인체섭취한계량의 0.03%, 0.08%, 0.0003% 수준이었다. 따라서, 국내 유통되는 인삼, 산양삼, 더덕 및 도라지 섭취로 인한 중금속 노출수준은 JECFA 등의 인체노출안전기준보다 매우 낮았으며 우리나라 국민은 다년생 근채류에 존재하는 중금속의 위해성으로부터 안전하였다.

• 한국환경농학회지
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• 제35권4호
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• pp.241-246
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• 2016

BACKGROUND: Recently, the widespread distribution of pesticides in the hive has been of concern about pesticide exposure on honeybee (Apis mellifera L.) health. Larval toxicity was adapted to assess the synergistic and antagonistic interaction of cumulative mortality to the honeybee larvae of the four most common pesticides detected in pollen. METHODS AND RESULTS: Acetamiprid($3.0{\mu}l/L$), chlorothalonil ($803.0{\mu}l/L$), coumaphos ($128.0{\mu}l/L$), and tau-fluvalinate ($123.0{\mu}l/L$) were tested in combination; binary, ternary and four component mixture. Larvae were exposed to four pesticides mixed in diet at the average levels detected in pollen. As a result, synthetic toxicity was observed in the binary mixture of acetamiprid with coumaphos. The binary and ternary component mixtures of tested pesticides have mostly demonstrated additive effect in larval bees. The significant antagonistic effects were found in four parings of mixtures including chlorothalonil added to acetamiprid/tau-fluvalinate or acetamiprid/coumaphos/tau-fluvalinate, and tau-fluvalinate added to acetamiprid/chlorothalonil or acetamiprid/coumaphos/chlorothalonil. CONCLUSION: Interactions between combinations of four pesticides showed mostly additive or antagonistic effects in larval bees. Therefore, predicting the larval mortality of pesticides mixtures on the basis of the results of single pesticide may actually overestimate the risk. We suggest that pesticide mixture in pollen be evaluated by adding their toxicity together for complete data on interactions.