Journal of the Korea Institute of Information and Communication Engineering
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v.21
no.4
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pp.829-835
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2017
Recently, the health issues triggered by fine dust matters occurred in higher frequency. Having adverse effects on health, particulate matters affect the human body indoors as well as outdoors. There is thus a need for a system to measure the concentration of particulate matters and control harmful particulate matters for human health in the indoor spaces where people live. The present study applied Internet of Things(IoT) technologies in order to increase the efficiency of the conventional fine dust measurement system. Especially, for the bidirectional communication environment, directly construct a separate server and applied to the system instead of a free cloud server also we used it directly in the school lab and home. When the proposed system is used in schools and homes, it can recognize the indoor environment quickly and it is expected that this will gradually contribute to the health of the individual. Users can also check the server data outside and deal with the current indoor situations.
The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.
Transactions on Electrical and Electronic Materials
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v.11
no.3
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pp.120-125
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2010
For the fabrication of PMOS and integrated semiconductor devices, B, $BF_2$ and dual elements with B and $BF_2$ can be implanted in silicon. 15 keV B ions were implanted in silicon at $7^{\circ}$ wafer tilt and a dose of $3.0{\times}10^{16}\;cm^{-2}$. 67 keV $BF_2$ ions were implanted in silicon at $7^{\circ}$ wafer tilt and a dose of $3.0{\times}10^{15}\;cm^{-2}$. For dual implantations, 67 keV $BF_2$ and 15keV B were carried out with two implantations with dose of $1.5{\times}10^{15}\;cm^{-2}$ instead of $3.0{\times}10^{15}\;cm^{-2}$, respectively. For the electrical activation, the implanted samples were annealed with rapid thermal annealing at $1,050^{\circ}C$ for 30 seconds. The implanted profiles were characterized by using secondary ion mass spectrometry in order to measure profiles. The implanted and annealed results show that concentration profiles for the ${BF_2}^+$ implant are shallower than those for a single $B^+$ and dual ($B^+$ and ${BF_2}^+$) implants in silicon. This effect was caused by the presence of fluorine which traps interstitial silicon and ${BF_2}^+$ implants have lower diffusion effect than a single and dual implantation cases. For the fabricated diodes, current-voltage (I-V) and capacitance-voltage (C-V) were also measured with HP curve tracer and C-V plotter. Electrical measurements showed that the dual implant had the best result in comparison with the other two cases for the turn on voltage characteristics.
Domestic sewerage treatment plant is operated by activated sludge method and its modified method by using microorganism. In most cases, a method of using microorganism is directly controlled by the operator based on individual judgment through factors of DO, pH and ORP. In addition, under aerobic condition in bioreactor, energy consumption including excessive air injection is learned to be somewhat plenty. In order to solve this problem, in most of the process, improvement of internal recycling and activated environmental factor of microorganism were researched extensively. However, as factors are changed depending on various conditions, it is not sufficient as an indicator of judgment. As such, a research on operation of bioreactor that measures metabolic change in short time by directly measuring activated condition of microorganism using NADH fluorometer is required in reality.It is considered that the method like this could supplement problem of energy consumption being occurred in the existing treatment method and operational optimization of bioreactor would be enabled by controlling optimal air volume. Therefore, in this study, in order to obtain optimal operational indicator of bioreactor, proper air volume control test was performed and through batch test and site evaluation, possibility of NADH sensor being utilized as operational control indicator of bioreactor is intended to be analyzed. In order to compare with measured value, DO, ORP that are operational control indicator of existing bioreactor were used. As MLSS concentration was increased through batch test, NADH value was increased and site evaluation also showed similar tendency to batch test. Resultantly, it could be confirmed that changing level of NADH fluorometer was a sensor that could measure bioreactor condition effectively and optimized scale of bioreactor is considered to be utilized.
Objectives : This study was conducted to investigate the inhibitory effects of Gaejibokryunghwan on cell proliferation in HeLa cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Gaejibokryunghwan extract solution. All three were cultured for 24 hours, 48 hours and 72 hours each, to examine the inhibitory effects of Gaejibokryunghwan. Afterwards, we drew out the effect of Gaejibokryunghwan extract solution by making 5 analysis. First analysis was to measure the proliferation rate of cells. Second was FACS analysis. Third was to estimate the activity or caspase-3. Fourth, we used XTT assay to analyze the activation or cells. Ana lastly, a molecular biological method was used to determine activation of MAP kinase in the HeLa cells. Results : After 24, 48 and 72 hours cultivation, the proliferation of HeLa cells showed the dose-dependent decrease in all Gaejibokryunghwan extract solution groups compared to the control group. In the FACS analysis, Gaejibokryunghwan extract solution groups showed increased caspase expression compared to the control group, except for the group for 48 and 72 hours in 1 % concentrate. Caspase-3 activities were increased in all, except tile group cultured for 24 hours in 5% concentrate and the groups cultured for 48 hours in 1% and 5% concentrate. In the XTT study, 1% Gaejibokryunghwan extract solution groups showed increase compared to the control group, but other Gaejibokryunghwan extract solution containing groups showed significant decrease compared to the control after 24, 48 and 72 hours of cultivation. The expressions of MAP kinase were decreased in all Gaejibokryunghwan extract solution containing groups compared to the control group after 24, 48 and 72 hours of cultivation. Conclusions : From this study, we could suggest that Gaejibokryunghwan be available to the inhibition of proliferation of human cervical carcinoma cell line, HeLa cells in vitro.
Kang, Hyunkyung;Roh, Hang Sik;Song, Hyemin;Lee, Kwangmoon;Chung, Seung-Tae;Ban, Sang-ja;Mo, In Pil;An, Beum-Soo;Ahn, Chi-Young
Toxicological Research
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v.32
no.4
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pp.269-274
/
2016
The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification of HA content by other alternative methods is required. In this study, we measured HA contents of H1N1 antigen and H1N1 influenza vaccine by reverse phase-high performance liquid chromatography (RP-HPLC) methods. The presence of HA1 and HA2 was investigated by silver staining and Western blot assay. In addition, accuracy and repeatability of HA measurement by RP-HPLC were evaluated. Comparison of HA concentration by SRID and RP-HPLC revealed a precise correlation between the two methods. Our results suggest that RP-HPLC assay can replace SRID in the event of a pandemic flu outbreak for rapid vaccine development.
There are lots of buildings with their deterioration in wholesale markets which have high concentration that can cause big fire due to difficulty of installing fire protection system in fire compartment, absence of safety awareness, access by many and unspecified people. There had been big fire that caused by these kind of fire weakness such as Seomun Market's in 2005 which costed tens of millions dollar, Dongmun Shopping District’s with 7 casualties. Now counter-measure against these fire is urgently needed. This study drew characteristic of current condition of wholesale markets and the one classified by main agent, fire cases and prevention of fire through statistical data. Also, current condition of fire prevention management and actual state were investigated by conducting a survey of person in charge of fire prevention and current practician. As a result, it was shown that securing professionalism of fire prevention, improvement of structural weakness of buildings, obtaining reliability on performance of fire protection system and internal fire education against practician in the market are needed.
This paper presents analysis of the interaction between tunnel and Qanat with a particular interest for the optimization of Qanat shape using the discrete element code, PFC2D, and the results will be compared with the FEM results of PLAXIS2D. For these concerns, using software PFC2D based on Discrete Element Method (DEM), a model with dimension of 100m ⁎ 100 m was prepared. A circular tunnel with dimension of 9 m was situated 20 m below the ground surface. Also one Qanat was situated perpendicularly above the tunnel roof. Distance between Qanat center and ground surface was 8 m. Five different shapes for Qanat were selected i.e., square, semi-circular, vertical ellipse, circular and horizontal ellipse. Confining pressure of 5 MPa was applied to the model. The vertical displacement of balls situated in ground surface was picked up to measure the ground subsidence. Also two measuring circles were situated at the tunnel roof and at the Qanat roof to check the vertical displacements. The properties of the alluvial soil of Tehran city are: γdry=19 (KN/㎥), E= 750 (kg/㎠), ν=0.35, c=0.3(kg/㎠), φ=34°. In order to validate the DEM results, a comparison between the numerical results (obtained in this study) and analytical and field monitoring have been done. The PFC2D results are compared with the FEM results. The results shows that when Qanat has rectangular shape, the tensile stress concentration at the Qanat corners has maximum value while it has minimum value for vertical ellipse shape. The ground subsidence for Qanat rectangular shape has maximum value while it has minimum value for ellipse shape of Qanat. The vertical displacements at the tunnel roof for Qanat rectangular shape has maximum value while it has minimum value for ellipse shape of Qanat. Historical shape of Qante approved the finding of this research.
Journal of the Microelectronics and Packaging Society
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v.25
no.3
/
pp.37-41
/
2018
This paper presents the integrated fluorescent oxygen sensor probe module based on planner lightwave circuits using UV imprint lithography. The oxygen sensor system is consisted of the optical source part, optical detector part and optical sensing probe part to be composed of the planner lightwave circuit and oxygen sensitive thin film layer. Firstly, we optimally designed the planner lightwave circuit with asymmetric $1{\times}2$ beam splitter using beam propagation method. Then, we fabricated the planner lightwave circuits using UV imprint lithography process. This planner lightwave circuits transmitted the optical power with 76% efficiency and the fluorescence signal with 70% efficiency. The oxygen sensitive thin film layer is coated on the end face of planner lightwave circuit. The oxygen sensor system using this sensor probe module with planner lightwave circuit could measure the concentration with 0.3% resolution from 0% to 20% gas range. This optical oxygen sensor probe module make it possible to compact, simple and cheap measurement system.
Proceedings of the Plant Resources Society of Korea Conference
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2012.05a
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pp.15-15
/
2012
Obesity is a physical condition that results from excessive storage of fat in the body. The present study examined the anti-obesity effects of the selected natural medicine, Galla rhois extract (GRE) and solvent fractions on 3T3-L1 preadipocytes and in vivo studies. Here, we show that EtOAc fraction of Galla rhois inhibits the differentiation of the 3T3-L1 preadipocytes induced by differentiated medium in a dose-dependent manner. To investigate the effect of the GRE-EtOAc fraction on obesity in high fat diet-fed C57BL/6 mice, which included a normal diet (ND), high-fat diet (HFD) and HFD+GRE concentration-dependent, were fed to the mice for 6 weeks. The GRE-EtOAc fraction was inhibited the highest adipocyte differentiation in vitro, the GRE supplement significantly decreased body weight and visceral fat mass compared to the HFD group. The total cholesterol and triglyceride levels in the plasma were significantly decreased by GRE supplementation compared with those of the HFD group. Also, we aimed to determine the differentiation inhibition and the modulation of differentiation genes brought about by the Galla rhois in adipocyte. A cDNA microarray-based method was introduced for the high contents screening (HCS) of gene expressions. This technology has revolutionized gene expression studies by providing the means to measure mRNA levels in thousands of genes simultaneously in simple and complex biological samples. 13 genes were founded to be affected in their expression levels by more than 5-fold up-regulation after 4 days treatment with the EtOAc fraction from Galla rhois. Otherwise, 21 genes were founded to be affected in their expression levels by more than 5-fold down-regulation treated with the EtOAc fraction. Therefore, Galla rhois extract may be considered for use in a therapeutic agent to control obesity.
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