• Title/Summary/Keyword: concentrated extracts

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Antibacterial effects of Corptis japonica against Helicobacter pylori (황련(黃連)의 Helicobacter pylori에 대한 항균 효과)

  • Seo Un-Kyo;Shin Jeong-In
    • The Journal of Internal Korean Medicine
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    • v.24 no.2
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    • pp.269-282
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    • 2003
  • Water and ethanol extracts of 67 species of medicinal plants were tested to determine antimicrobial activity against Helicobacter pylori. Among them, the extracts of Coptis japonica showed the best antibacterial activity. The extract of C. japonica showed four major spots on TLC plate and the Rf values of the spots were 0.07, 0.13, 0.21 and 0.73, respectively. Except for the spot of Rf 0.73, other three spots inhibited the cell growth of H. pylori. As shown in HPLC analysis, three antimicrobial spots contain berberine, major antimicrobial substance of C. japonica. However, the spot of Rf 0.13 had higher activity than berberine. The concentrated water extract of three prescribed medicines related with C. japonica showed good antibacterial activity against H. pylori.

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Phenolic Compounds from Fruits of Amorpha fruticosa L.

  • Lee, hak-Ju;Lee, Hyun-Jung;Park, Il-Kwon;Shin, Sang-Chul;Lee, Myung-Koo;Paik, Ki-Hyon
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.190.4-191
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    • 2003
  • This study was carried out to examine the constituents of Amorpha fruticosa (Leguminosae), a shrub originated from North Africa. Dried and ground fruit of A. fruticosa were extracted with methanol and then concentrated to give the crude extracts. The crude extracts was successively fractioned with organic sovents, such as n-hexane, CH$_2$Cl$_2$ and EtOAc. Seven compounds were isolated from the fruits of A. fruticosa. On the basis of spectrosopic data, the structures of these compounds were determined as: kaempferol 7-O-${\alpha}$-L-rhamnopyranoside (I), methyl 3,4,5- trihydroxybenzoate (methyl gallate, II), tephrosin (III), dalbinol (IV), gallic acid (V), 2",4",5",7-tetramethoxyisofavone (Ⅵ) and Dalbinol 2"-O-${\beta}$-D-Glucopyranoside (Ⅶ) respectively. (omitted)

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CMOS 영상센서에 대한 영상 신호 전처리기의 구현

  • 정영식;장영조
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2001.12a
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    • pp.15-18
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    • 2001
  • Recently, CMOS image sensor is rapidly used as an image capture device such as mobile phone or notebook PC. Because of poor quality of image by CMOS image sensor, ISP is essential step to improve image quality. In this paper, we implemented and simulated ISP algorithm for real time moving picture of CMOS image sensor. Especial Iy, we concentrated on color interpolation, which extracts three color component from uncompleted color information. Several algorithms for color interpolation are implemented and analyzed to acquire a good quality of picture. Finally, we proposed an improved algorithm and confirmed the effectiveness by experimental simulation results.

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A Study of Effects of Ilex serrata Thunb Extracts (낙상홍 추출물의 효능에 관한 연구)

  • Lee, Kwang-Soo;Park, Kyung-Sook
    • The Korean Journal of Food And Nutrition
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    • v.29 no.6
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    • pp.946-951
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    • 2016
  • In this study, Ilex serrata Thunb was extracted with 70% methanol and concentrated under reduced pressure to measure its total polyphenol contents; furthermore, we examined the effect of electron donating ability of the various extracts. The extract was fractionated using different solvents such as methylene chloride, ethyl acetate, and methanol to investigate total polyphenol contents and electron donating abilities. The total polyphenol contents were $1,058.59{\pm}85.85{\mu}g\;GAE/mL$ in 70% methanol, $297.29{\pm}29.43{\mu}g\;GAE/mL$ in methylene chloride, $313.28{\pm}9.22{\mu}g\;GAE/mL$ in ethyl acetate, and $837.82{\pm}31.16{\mu}g\;GAE/mL$ in methanol. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating abilities were $72.93{\pm}0.29%$ for 70% methanol extract, $14.02{\pm}2.01%$ for methylene chloride, $24.05{\pm}1.75%$ for ethyl acetate, and $84.08{\pm}1.95%$ for methanol. The electron donating abilities were significantly different (p<0.05) between the solvents. The two portions of Ilex serrata Thunb extracts obtained from methanol showed significantly relevant results in the total polyphenol contents and electron donating ability, which was higher than the original extract.

Antifungal Activity of Extract of Common Purslane (Portulaca oleracea L.) (쇠비름 즙액(汁液)의 항균작용(抗菌作用))

  • Park, Jong Seong;Kwon, Jin Sook;Lee, Kyu Seung
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.190-193
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    • 1984
  • Extracts of common purslane(Portulaca oleracea L.) showed to possess some antifungal substances which inhibited the mycelial growth of the phytopathogenic fungi tested;Valsa mali, Alternaria kikuchiana and Pyricularia oryzae. These antifungal substances were found to be soluble in methanol and were regarded as kinds of lipid. In order to isolate the antifungal substances, the extracts of common purslane were concentrated by evaporation under reduced pressure and extracted with methanol The methanol solution was subjected to silica gel-florisil column and divided into lipid and non-lipid fractions. Lipid fractions only showed antifungal activity against the fungi tested. The effective substances contained in the extracts of common purslane inhibited not only the mycelial growth but also the spore germination of the fungi.

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Reduction of Stress Caused by Drought and Salt in Rice (Oryza sativa L.) Crops through Applications of Selected Plant Extracts and the Physiological Response Mechanisms of Rice

  • Hyun Hwa Park;Young Seon Lee;Yong In Kuk
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.57-57
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    • 2022
  • In many areas of the world, salt damage and drought have had a negative impact on human survival due to a decrease in agricultural productivity. For instance, about 50% of agricultural land will be affected by salt damage by 2050. Biostimulants such as plant extracts can not only increase the nutrient utilization efficiency of plants, but also promote plant growth and increase resistance to abiotic or biotic stress. Therefore, the objective of this study was to determine how selected plant extracts might reduce levels of stress caused by drought and salt and to better understand the physiological response mechanisms of rice plants. In this study, we used Soybean leaves, Soybean stems and Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts were used. These extracts had been used in previous studies and were found to be effective. The materials were dried in a dry oven at 50℃ for 5 days and ground using a blender. Each 50 g of materials was put in 1 L of distilled water, stirred for 24 hours, filtered using 4 layers of mirocloth, and then concentrated using a concentrator. Rice (cv. Hopumbyeo) seeds were immersed and germinated, and then sown in seedbeds filled with commercial soil. In drought experiments, three rice seedlings at 1 week after seeding was transplanted into 100 ml cups filled with commercial soils and grown until the 4-leaf stage. For this experiment, the soil weight in a cup was equalized, and water was allowed to become 100% saturated and then drained for 24 hours. Thereafter, plant extracts at 3% concentrations were applied to the soils. For NaCl treatments, rice plants at 17 days after seeding were treated with either 100 mM NaCl or plant extracts at 1%+ 100 mM NaCl combinations in the growth chamber. Leaf injury, relative water content, photosynthetic efficiency, and chlorophyll contents were measured at 3, 5, and 6 days after treatments. Shoot fresh weight of rice under drought conditions increased 28-37% in response to treatments of Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Shoot fresh weight of rice subjected to 100 mM NaCl treatments also increased by 6-24% in response to Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Compared to the control, rice plants treated with these six extracts and subjected to drought conditions had significantly higher relative water content, Fv/Fm, total chlorophyll and total carotenoids than control plants. With the exception of relative water contents, rice plants treated with the six extracts and subjected to salt stress (100 mM NaCl treatments) had significantly higher Fv/Fm, total chlorophyll and total carotenoids than control plants. However, the type of extract used did not produce significant difference in these parameters. Thus, all the plant extracts used in this study could mitigate drought and NaCl stresses and could also contribute substantially to sustainable crop production.

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Screening of Inhibitors of Extracellular Serine Protease of Acanthamoeba castellanii from Mushroom Extracts (버섯 추출물로부터 Acanthamoeba castellanii의 세포외 Serine 단백질분해효소 저해제 탐색)

  • Lee, Seung-Eun;Sancheti, Sandesh;Sancheti, Shruti;Choi, Mie-Young;Seo, Sung-Yum
    • The Korean Journal of Mycology
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    • v.36 no.2
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    • pp.178-182
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    • 2008
  • Although the number of patients with Acanthamoeba keratitis has increased dramatically since the widespread use of contact lens, it is still very hard to cure the disease. The proteases from the Acanthamoeba were reported to play important role in the pathogenesis of keratitis. In this study, the inhibitors for extracellular serine proteases of A. castellanii were screened from the extracts of 230 mushroom samples collected from various regions of Korea. The mushrooms were extracted with methanol and water ($65^{\circ}C$). Filtered and concentrated extracts (0.3 mg/ml) were preincubated with proteases before addition of peptide substrate N-succinyl-ala-ala-pro-phe p-anilide. The selected extracts showing strong inhibitory effects were characterized. Although inhibition with single extract was not so high enough, the complete inhibition was achieved with combination of two extracts. The selected extract showed little effect on other serine proteases such as thrombin (human and bovine) and on general protease such as protease K.

Effects of Injinsaryung-san on Experimental Liver Damage in Rats (인진사령산이 흰쥐 간손상(肝損傷)에 미치는 영향(影響))

  • Pyo, Im-Jeong;Lee, Jang-Hun;Woo, Hong-Jong;Kim, Byung-Woon
    • The Journal of Korean Medicine
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    • v.16 no.2 s.30
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    • pp.281-298
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    • 1995
  • The following results were made by observation GOT, GPT, ALP, total cholesterol and higlyceride in serum to research the effects of medicines which are Injinsaryungsan(Sample- A) and another medicine(Sample-B) of which Injin(Artemisiae Capillaris Herba)was increased in quantity on liver damaged by $CCl_4$ and d-galactosamine in rats. 1. The high concentrated extracts of Sample A group and Sample- B group showed significant inhibltory effects on the increase of serum GPT, ALP, LDH, total cholesterol and triglyceride levels induced by $CCl_4$ and d-galactosamine. 2. The high concentrated extracts of sample A group and Sample B group showed more significant inhibitory effects(P〈0.001) than the low ones' effects(P〈0.01) on the increase of serum triglyceride level induced by $CCl_4$ 3. Sample-A group showed significant inhibitory effects on the increase of serum GOT, GPT. ALP, total cholesterol and triglyceride levels, but no significance on the increase of serum LDH level induced by d-galactosamine. 4. Sample-B group showed very significant inhibitory effects on the increase of serum GOP, GPT, ALP, LDH, total cholesterol and triglyceride levels induced by d-galactosamine. 5. As compared with Sample-A group, Sample-B group of which Injin was increased in quantity showed more significant inhibitory effects on all items of this experiment induced by $CCl_4$ and d-galactosamine. As mentioned above. it seemed that both Injinsaryungsan and another medicine of which Injin was increased in quantity had effects protecting liver and anti-fatty liver induced by $CCl_4$ and d-galactosamine in rats. Specially Sample-B group had very significant effects on liver damage as compared with Sample-B group. Therefore it seems that more researches on variation according to the increase of Injin dose must be continued for curing liver diseases.

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Inhibitory Effect of Oriental Herbal Medicines on Tumor Necrosis $Factor-{\alpha}$ Production in Lipopolysaccharide-stimulated RAW264.7 cells

  • Cho, Jae-Youl;Park, Ji-Soo;Kim, Pyung-Su;Chae, Sook-Hee;Yoo, Eun-Sook;Baik, Kyong-Up;Lee, Jong-Soo;Park, Myung-Hwan
    • Natural Product Sciences
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    • v.5 no.1
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    • pp.12-19
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    • 1999
  • Eleven out of 118 herbal medicines which are frequently used in Korean traditional prescriptions for inflammatory diseases exhibited more than 50% of inhibition on $TNF-{\alpha}$ production in LPS-stimulated RAW264.7 cells by their total ethanol extracts with 0.1 mg/ml as a final concentration. The active 11 total extracts were prepared from Angelica koreana, Coptis japonica, Cynanchum paniculatum, Magnolia frgesii, Magnolia officinalis, Panax ginseng, Patrinia scabiosaefolia, Pterocarpus santalius, Rhapontica uniflora, Saussurea lappa. Of them, Coptis japonica, Magnolia fargesii and Saussurea lappa also significantly inhibited $TNF-{\alpha}$ production in vivo. These total extracts were sequentially fractionated with n-hexane, methylene chloride, ethyl acetate, n-butanol and water. Among the solvent-fractionated extracts with 0.05 mg/ml as a final concentration, inhibitory effects of Angelica koreana, Magnolia fargesii, Magnolia officinalis, Pterocarpus santalinus, Rhapontica uniflora and Saussurea lappa markedly showed in one or two solvent fractions suggesting that the principles may be concentrated by subfractionation as the main compounds.

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Comparative Phytochemical Profiling of Methanolic Extracts of Different Parts of White Dandelion (Taraxacum coreanum) using Hybrid Ion-mobility Q-TOF MS

  • Hyemi Jang;Mira Choi;Eunmi Lee;Kyoung-Soon Jang
    • Mass Spectrometry Letters
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    • v.15 no.2
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    • pp.95-106
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    • 2024
  • Taraxacum coreanum, known as the native Korean white dandelion, has been historically used in traditional medicine due to its various therapeutic properties. However, the specific benefits and mechanisms of white dandelion in alleviating particular symptoms or diseases remain uncertain due to the complexity of its phytochemical profile. In this study, we aimed to elucidate the phytochemical profiles of methanolic extracts of different parts of the white dandelion (flower, leaf, stem, and root) using hybrid ion-mobility Q-TOF MS. Using the trapped ion mobility-based PASEF technique, 3715 and 2114 molecular features with MS2 fragments were obtained in positive and negative ion modes, respectively, and then a total of 360 and 156 phytochemical compounds were annotated by matching with a reference spectral library in positive and negative ion modes, respectively. Subsequent feature-based molecular networking analysis revealed the phytochemical differences across the four different parts of the white dandelion. Our findings indicated that the methanolic extracts contained various bioactive compounds, including lipids, flavonoids, phenolic acids, and sesquiterpenes. In particular, lipids such as linoleic acids, lysophosphatidylcholines, and sesquiterpenoids were predominantly present in the leaf, while flavonoid glycosides and lysophosphoethanolamines were notably enriched in the flower. An assessment of the total phenolic content (TPC) and total flavonoid content (TFC) of the methanolic extracts revealed that the majority of phytochemicals were concentrated in the flower. Interestingly, despite the root extract displaying the lowest TPC and TFC values, it exhibited the highest radical scavenging rate when normalized to TPC and TFC, suggesting a potent antioxidant effect. These findings and further investigations into the biological activities and medicinal potential of the identified compounds, particularly those exclusive to specific plant parts, may contribute to the development of novel therapeutic agents derived from white dandelion.