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Effect of Overhead Flooding Stress on Protein Profiles, Fatty Acid Composition, and Lipid Peroxidation in Rice (벼의 관수기간 및 수질이 단백질, 지방산조성 및 지질과산화에 미치는 영향)

  • 이상각;이병모;강병화
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.3
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    • pp.215-220
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    • 2001
  • Biochemical responses of rice to the flooding stress with different water turbidities and flooding time were evaluated. About 20% decrease of soluble protein was occurred with flooding stress. The decreasing rate was higher as flooding time and higher turbidity increased. Especially, dramatic decrease of soluble protein content was observed after 36 hrs of flooding. No protein subunit change was found before and after flooding. However, subunit product of 53 Kd increased from the beginning of flooding and subunit of 28 Kd was increased 48 hrs and 54 hrs after flooding. Lipid peroxidation increased about 150% by flooding. There was no significant difference in lipid peroxidation between clear and sub-muddy water, However, the lipid peroxidation was increased up to 180% at 60hrs of flooding. The malondialdehyde content (MDA) was higher in muddy water at the beginning of flooding and increased about 190-200% 36 hrs after flooding. Catalase activity increased with increasing turbidity and flooding time. Forty eight hours of flooding time provided a criteria for dramatic increase of catalase activity. In general, increase of saturated fatty acids and decrease of unsaturated fatty acids occurred with flooding treatment. Among unsaturated fatty acids, monounsaturated increased and polyunsaturated decreased. Double bond index(DBI) decreased as flooding time was extended and turbidity increased.

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The Effect of Additives in the Cardioplegic Solution on the Recovery of Myocardium, Compariosn Among Albumin, Mannitol, and Glucose (심마비용액의 삼투압을 유지하기위한 첨가 물질들의 차이가 심근보호에 미치는 영향)

  • Kim, Eun-Gi;Lee, Jong-Guk;Lee, Sang-Heon
    • Journal of Chest Surgery
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    • v.24 no.11
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    • pp.1058-1067
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    • 1991
  • High potassium cardioplegia is a widely accepted procedure to enhance myocardial protection from ischemic injuries associated with open heart surgery. Maintaining optimum osmolarity of the cardioplegic solution is one of the required conditions for an ideal cardioplegic solution Albumin is an frequently added component for maintaining optimum osmolarity of clinically used cardioplegic solutions. But the source of albumin is human blood so that the supply is limited and the cost of manufacturing is relatively high. Recently there are moves to minimized the use of blood product for fear of blood-associated infections or immunological disorders. In this experiment, we substituted mannitol or glucose for albumin added to the cardioplegic solution which has been used at the Wonju Medical College, To determine whether addition of mannitol or glucose instead of albumin in the cardioplegic solution can produce satisfactory myocardial protection during ischemia, three different groups of isolated rat heart perfused by modified Langendorff technique were studied. Wonju Cardioplegic Solution was selected as a standard high potassium[18mEq/L of K+] cardioplegic solution. Three kinds of cardioplegic solution were made by modifying the composition maintaining the same osmolarity[339$\pm$1mOsm/Kg] Isolated rat heart were perfused initially with retrograde nonworking mode and then changed to working mode. After measuring the heart rate, systolic aortic pressure, aortic flow, coronary flow, ischemic arrest by aorta cross clamp and cardioplegia was made maintaining the temperature of water jacket at 10oC. The heart was rewarmed and reperfused after 60min of ischemic arrest with intermittent cardioplegia at the 30min interval. The time to return of heart beat and the time required to get. Regular heart beat were observed after reperfusion. The recovery rate of the functional variables-heart rate, systolic aortic pressure, aortic flow, coronary flow and cardiac output were calculated and compared among the three groups of different cardioplegia-albumin, mannitol, and glucose. The wet weight and dry weight was measured and the water content of the heart as figured out for comparison. The time to return of heart beat was fastest in the albumin group, The functional recovery rates were best in the albumin group also. In the above conditions, albumin was the best additive to the cardioplegic solution compared to the mannitol or glucose.

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A Study on Photoluminance Properties of $(Y,Gd)BO_3:Eu^{3+}$ Phosphor Synthesized by Ultrasonic Spray Pyrolysis (초음파 분무법으로 제조한 $(Y,Gd)BO_3:Eu^{3+}$ 형광체의 발광특성에 관한 연구)

  • Kim, Dae-Su;Lee, Rhim-Youl
    • Korean Journal of Materials Research
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    • v.10 no.3
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    • pp.204-211
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    • 2000
  • The $(Y,Gd)BO_3:Eu$ red phosphors for PDP application were synthesized by ultrasonic spray method and then their photoluminance properties were investigated under 147nm VUV irradiation. The precursor solution of acetates of Y, GD and Eu and boric acid diluted in water was sprayed using 1.7 MHz ultra-sonic sprayer into the reaction tube held at high temperature. The as-sprayed particles were amorphous phase having C-C and C-H bonds due to the insufficient thermal reaction during the pass along the tube. But the sprayed samples followed by heat treatment at $1100^{\circ}C$ had the same crystal structure and chemical composition as those samples followed by solid state reaction. It was found that the $(Y_{0.7}Gd_{0.3})_{0.95} BO_3:Eu_{0.05}^{3+}$ phosphor particles synthesized by spray at $500^{\circ}C$ and then heat treated at $900^{\circ}C$ had a spherical-like shape and fine particle size at $0.7{\mu\textrm{m}}$ having a narrow size distribution, while the phosphor particles made by solid state reaction was $3{\mu\textrm{m}}$ coarse and non-uniform size distribution. The emitting intensity under 147nm VUV excitation for $(Y_{0.7}Gd_{0.3})_{0.95}BO_3:Eu_{0.05}^{3+}$ phosphor prepared by spray method was found to be higher than those phosphor made by solid state reaction and the commercial $(Y,Gd)BO_3:Eu$ product.

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Identification and Formation Factor of White Crystals on the Excavated Costumes from Shim Su-Ryun's Tomb (심수륜 묘 출토복식에서 발견되는 백색 결정의 동정 및 생성 요인)

  • Lee, Young Eun;Choi, Seokchan
    • Conservation Science in Museum
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    • v.13
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    • pp.37-44
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    • 2012
  • White crystals on 46 costumes excavated from Shim Su-Ryun(1534 - 1589)'s tomb were examined their characterization and distribution. In 36 of such samples, white crystals with different shape and hardness were found. The formation of crystals did not correlated with a kind and use of textiles. However, crystals were found in the back side than the front of costume, specially around the marks of shrouding dead body. White crystals from 7 textiles were investigated by EPMA, XRD, or FT-IR. The composition of white crystal was analysed by EPMA and the structure characterization of crystals was used by X-ray diffraction. FT-IR spectroscopy was applied to check if non-crystalline compounds were also present. Mg and P were detected as the main element of white crystals and these compounds were identified a struvite and newberyite, the inorganic mineral magnesium ammonium phosphates. Struvite precipitation are influenced by many factors including concentration of Mg2+, NH4+, and PO43- ions, pH, and temperatures. It is assumed that magnesium, phosphorous, ammonia, a base material of struvite comes from decomposition product of human body. Tomb covered with lime, a unique triple-structure in Joseon period offering the basic condition, an anaerobe in a coffin, and high magnesium concentration of outer coffin with lime can be inferred as important factor for precipitation of crystals.

Mycelial Growth and Fruiting Body Formation of Hericium erinaceum in Sawdust and Agricultural By-product Substrates (톱밥 및 농업부산물 이용 배지상에서 노루궁뎅이버섯(Hericium erinaceum)의 균사생장 및 자실체형성)

  • Ko, Han-Gyu;Park, Hyuk-Gu;Kim, Seong-Hwan;Park, Won-Mok
    • The Korean Journal of Mycology
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    • v.32 no.2
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    • pp.89-94
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    • 2004
  • This study was carried out to investigate the suitability of various agricultural by-products as basal substrates for the mycelial growth and fruiting body formation of Hericium erinaceum. For this aim, oak sawdust, cotton waste, sugarcane bagasse, Job's tears, rice hull, Chinese cabbage, and coconut waste were used as sole or mixed substrate(s). Corn waste and rice bran were used as nutrient supplements. The growth and density of mycelium, yield of fruiting body, and biological efficiency were compared among tested substrates colonized by Hericium erinaceum. The best measurement of mycelial growth and density, yield of fruiting body, and biological efficiency in a laboratory test was found in a spawn substrate composed with oak sawdust 80% and rice bran 20%. The suitability of this spawn substrate composition for Hericium fruiting body production was testified through practical tests in plastic bottles (850 ml) in a mushroom farm which had bottle cultivation facility. However, test in a mushroom farm which had plastic bag cultivation facility, best production of Hericium fruiting body (520 g per one bag) was observed in a spawn substrate composed of cotton waste 40%, saw dust 40%, corn waste 10%, and rice bran 10%.

The Effect of Drug Release from Osmotic Pellet Related to the Various Ratio of $Eudragit^{(R)}$ RL and RS ($Eudragit^{(R)}$ RL과 RS의 비에 따른 삼투정 펠렛의 약물방출에 미치는 영향)

  • Youn, Ju-Yong;Ku, Jeong;Lee, Soo-Young;Kim, Byung-Soo;Kim, Moon-Suk;Lee, Bong;Khang, Gil-Son;Lee, Hai-Bang
    • Polymer(Korea)
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    • v.31 no.4
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    • pp.329-334
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    • 2007
  • Osmotic pellet system, which is one of the oral drug delivery systems, has been developed to improve manufacturing process, reduce product cost and other problems of osmotic tablet systems. Osmotic pellet is consisted of water swellable seed layer, drug layer, and membrane layer. Among them, the membrane layer plays an important role in a control of the drug release. In this work, we examined the effect of ratio for Eudragit RL and RS on the drug release behavior. Osmotic pellet with nifedipine as a model drug was easily obtained in a good yield by fluidized bed coater. Osmotic pellet showed round morphology with a range of size $1300{\sim}1500\;{\mu}m$. In the experiment of nifedipine release, the release amount increased with the increase of the ratio of Eudragit. This is due to the fact that Eudragit RL contains more hydrophilic quaternary ammonium group than Eudragit RS. Additionally, the release amount was retarded with increasing the membrane thickness. There are no differences in the release amount measured at the different pH 1.2, 6.5, 6.8, and 7.2. In conclusion, it was found that the drug release from osmotic pellets depended on the composition ratio and coating thickness of membrane layer.

The Conversion of Methane with Oxygenated Gases using Atmospheric Dielectric Barrier Discharge (배리어방전을 이용한 메탄전환반응에서 함산소 가스가 전환율 및 생성물변화에 미치는 영향)

  • Lee Kwang-Sik;Yeo Yeong-Koo;Choi Jae-Wook;Lee Hwa-Ung;Song Hyung-Keun;Na Byung-Ki
    • Journal of Energy Engineering
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    • v.15 no.1 s.45
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    • pp.52-59
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    • 2006
  • This paper examined the conversion of methane to hydrogen and other higher hydrocarbons using dielectric barrier discharge with AC pulse power. Two metal electrodes of a coaxial-type plasma reactor were separated by gas gap and an alumina tube. The inner electrode was located inside the alumina tube. The alumina tube was located inside the stainless steel tube, which was used as the outer electrode. Effect of feed gas composition (methane, oxygen, argon, water and helium), flow rate, applied frequency, input volt-age on methane conversion and product distribution were studied. The major products of plasma chemical reactions were ethylene, ethane, propane, buthane, hydrogen, carbon monoxide and carbon dioxide. The increment of applied voltage and the usage of inert gas as the background (helium and argon) enhanced the selectivity of hydrocarbons and methane conversion. The addition of water in the feed stream enhanced the conversion of methane and yield of hydrogen. Higher voltage leads to higher yield of $C_2H_6,\;C_3H_8,\;C_4H_{10}$ and yield or $C_2H_2\;and\;C_2H_4$ appeared highly in lower voltage.

A Study of Exploiting Raw Material of Seasoning by Using Fish and Shells -1. On Composition of Seasoning Material in Cooking By-product- (어패류를 이용한 조미료 소재 개발에 관한 연구 -1. 패류 자숙액즙의 성분조성-)

  • KIM Woo-Jun;BAE Tae-Jin;CHOI Jong-Duck;CHOI Ji-Hyun;AHN Myung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.3
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    • pp.259-264
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    • 1994
  • The possibility of shellfish utilization of juice as seasoning materials was examined through the analyzing proximate compositions and N-containing materials. The amounts of the sucking liquid wastes were estimated as 58,819 tons for oyster, 764 tons for pen-shell, 604 tons for cockle and 3,896 tons for blue-mussel from 1985 to 1990 in Korea. Water contents ranged from 95.2 to $97.4\%$ and crude protein level was $1.0{\sim}1.4\%$. Taurine was the most abundant nitrogenous compound($40{\sim}56\%$) in extracts, and glycine($10.4{\sim}36.1\%$), alanine($4.8{\sim}8.6\%$) and glutamic acid($3.1{\sim}5.3\%$) followed. More than $40\%$ of ADP and AMP as mucleotide were contained in all shellfish samples but pen-shell and cockle had $20\%$ of inosine, also. Glycinebetaine was present in corcentrations of 139.8mg/g in oyster and $45.5{\sim}61.9mg/100g$ in pen-shell, cockle and blue-mussel. Total creatinine and trimethylamine oxide levels were $30.4{\sim}59.1mg/100g$ and $0.82{\sim}47.2mg/100g$, respectively.

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A Study of the Microstructure and Impurity Characteristics of Cast Bronze in Koryo Period (고려시대의 청동 주물에서 관찰되는 불순물(Cu2S) 특성 연구)

  • Choi, Jung Eun
    • Journal of Conservation Science
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    • v.32 no.3
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    • pp.313-320
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    • 2016
  • The aim of this study was to obtain information on the ancient material of cast bronze through an investigation of the microstructure and impurity characteristics of the casting. Three Koryo bronze coins were analyzed using an optical microscope, scanning electron microscope, and electron dispersive X-ray analyses were used to determine the composition of the specimens. The three coins had 4 phases: ${\alpha}phase$, ${\delta}phase$, Pb, and impurities ($Cu_2S$). $Cu_2S$ was found to exist near Pb or in ${\delta}phase$. $Cu_2S$ is the inter mediate product of copper ore refining. Therefore, the copper ore was not completely refined. To find out the characteristic of $Cu_2S$, we melt 1)Koryo bronze coin and 2)$Cu_2S$ and Pb powder at 1273 K. The reaction between $Cu_2S$ and Pb at 1273 K yielded fine Cu and black gas, which was identified to be PbS and is presented below: $Cu_2S+Pb{\rightarrow}PbS{\uparrow}+2Cu$.

A Study on the Characteristics of Consumer Visual-Perceptional Information Acquisition in Commercial Facilities in Regard to its Construction of Space from Real-Time Eye Gaze Tracking (상업시설 공간구성의 실시간 시선추적에 나타난 소비자 시지각 정보획득 특성 연구)

  • Park, Sunmyung
    • Science of Emotion and Sensibility
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    • v.21 no.2
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    • pp.3-14
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    • 2018
  • For satisfying consumer needs, commercial facilities require a variety of sale-related space expressions and eye-catching product arrangements; space composition can also be a direct marketing strategy. The human eye is the sensory organ that acquires the largest amount of information, and an analysis of visual information helps in understanding visual relations between . However, the existing studies are mostly focused on analysis of still frames in experimental images, and there is a lack of studies analyzing gaze information based on mobile images of commercial spaces. Therefore, this study analyzed emotional responses through gaze information of space users in reality using a video of a movement route through a commercial facility. The analysis targeted straight sections of the moving route; based on the data acquired, sectional characteristics of five gaze intensity ranges were examined. As a result, section A, the starting point of the route, had a low gaze intensity, while section B had the highest gaze intensity. This indicates that, starting in section B, the subjects explored the space in a stable way and needed time to adapt to the experimental video. In relation to space characteristics of the gaze-concentrated area, display formats of the right stores in 4 of 6 sections received greater attention. The gaze of consumers was mostly focused on props, and big gaze information was revealed in showcase display formats of the stores. In conclusion, this analysis method can provide highly useful direct design data about merchandise display and merchandise component arrangement based on consumer visual preference.