• 한국융합학회논문지
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• 제12권11호
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• pp.235-245
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• 2021

4차 산업혁명시대를 맞이하여 과거보다 복잡해진 광고홍보산업 생태계의 복합적인 문제들이 제기되면서, 융합교육의 필요성과 중요성이 요구되고 있다. 본 연구는 학계와 업계의 팀티칭 교수법을 통한 융합교육과 관련된 문헌연구와 광고홍보 학계와 산업계 전문가를 대상으로 한 심층인터뷰를 실시하여 융합교육 활성화를 위한 팀티칭 교수법의 유용성과 실행방안을 연구하였다. 연구결과, 광고홍보학과 교과과정에서 학계와 업계가 공동으로 참여하는 팀티칭 방법의 도입은 적절하며, 학계와 업계의 두 명 이상의 교육자가 공동으로 교육 프로그램을 진행하는 팀티칭은 이론과 실무를 융합한 문제해결 능력 역량을 갖춘 학생 양성에 목적을 두고 있는 의미 있는 교육방법이 될 것으로 나타났다. 본 연구는 광고홍보학과 팀티칭 교육의 탐색적 연구로 새로운 산학 협동 모델을 제안하였으며, 향후 학계와 업계에서 지속적인 연구가 필요할 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1228-1237
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• 2023

The CRISPR-Cas system has emerged as the most efficient genome editing technique for a wide range of cells. Delivery of the Cas9-sgRNA ribonucleoprotein complex (Cas9 RNP) has gained popularity. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR)-based assay to quantify the double-strand break reaction mediated by Cas9 RNP. To accomplish this, the dextransucrase gene (dsr) from Leuconostoc citreum was selected as the target DNA. The Cas9 protein was produced using recombinant Escherichia coli BL21, and two sgRNAs were synthesized through in vitro transcription to facilitate binding with the dsr gene. Under optimized in vitro conditions, the 2.6 kb dsr DNA was specifically cleaved into 1.1 and 1.5 kb fragments by both Cas9-sgRNA365 and Cas9-sgRNA433. By monitoring changes in dsr concentration using qPCR, the endonuclease activities of the two Cas9 RNPs were measured, and their efficiencies were compared. Specifically, the specific activities of dsr365RNP and dsr433RNP were 28.74 and 34.48 (unit/㎍ RNP), respectively. The versatility of this method was also verified using different target genes, uracil phosphoribosyl transferase (upp) gene, of Bifidobacterium bifidum and specific sgRNAs. The assay method was also utilized to determine the impact of high electrical field on Cas9 RNP activity during an efficient electroporation process. Overall, the results demonstrated that the qPCR-based method is an effective tool for measuring the endonuclease activity of Cas9 RNP.

• 기술혁신학회지
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• 제6권4호
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• pp.551-568
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• 2003

The aircraft industry is a typical complex product system (CoPS) and one of the system integration industries. In this particular sector, industrial competitiveness is decided by technological capabilities in system level as well as subsystem level. Korea has been involved in the aircraft industry since early 1970s. Technological capability in the system level has been developed based on military aircraft production program. However, subsystem and component level capabilities have been remained less developed. In this paper, the capability building process as well as current problem; of the Korean aircraft industry have been examined. In conclusion, capability building up strategies in the Korean aircraft industry has been suggested.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.210-212
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• 2015

The growth-share matrix is a portfolio planning tool developed by the Boston Consulting Group (BCG) to assist competitive positioning in the international market including those in the construction industry. This matrix is helpful in balancing the firm's cash-flow, and it can suggest strategic directions for each business unit. However, its effectiveness and applicability have long been debated in the academic field due to the complex and unique industrial context of construction. To solve the dispute, this research clarifies the applicability of theories underlying the growth-share matrix to the construction industry. Empirical research based on actual financial data of Korean construction firms is adopted for the statistical analysis including one-way analysis of variance and correlation analysis. The results of this research show that empirical findings on the relationship between performance variables. In this context, this research can provide important insights on the concept of portfolio management in the construction industry.

• 방사선산업학회지
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• 제5권2호
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• pp.159-164
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• 2011

A variety of natural polymers have been used as tissue engineering scaffolds, drug delivery system, and cosmetic materials due to their higher biocompatibility and water uptake. As a major component of extracellular matrix, hyaluronic acid consisting of D-glucuronic acid and N-acetylglucosamine has been popularly used as a hydrogel material. Even though it has good properties to be used in the tissue engineering and cosmetic industry, its higher viscosity has limited a potential use in a variety of applications; only low content should be applied in preparing above products. In the present study, we investigated the effect of electron beam irradiation on the properties of hyaluronic acid. Hyaluronic acid paste containing low contents of water changed to solution after electron beam irradiation ranging from 1 to 10 kGy, which didn't exhibit any alteration of surface properties and morphological change after freeze-drying. However, its viscosity was significantly decreased as absorbed dose increased, which was approximately one by hundred in comparison with the viscosity of original hyaluronic acid solution with same concentration. In addition, it can still interact with positive charged chitosan generating polyelectrolyte complex. Therefore, only viscosity was decreased after electron beam irradiation, whereas other properties of hyaluronic acid maintained. Consequently, these hyaluronic acids with lower viscosities can be used in a variety of applications in tissue engineering, drug delivery, and cosmetic industry.

• 한국주거학회논문집
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• 제25권6호
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• pp.27-37
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• 2014

The purpose of this study was to identify the perception on social mix of managers and residents in the mixed housing complexes for sale and rental apartment. For the research purpose, concept and background of social mix, relevant policies, preceding research trend of social mix were organized through the literature analysis. In addition, observation study was executed to examine physical aspects of three selected sites as research subjects. Also, current state and awareness for social mix were conducted by using in-depth interview with 6 managers and 30 residents in selected areas with semi-structured questionnaires from June 3 to 7, 2013. The results of the study are as following: In the aspect of management, managers said that the biggest issue of mix-housing complex was dualization of management subject such as delegate of residents and tenants. Therefore, legislation which is suitable for unique features of mix-housing complex is required. To decrease disharmony between condominium and rental apartment, controlling the distribution ratio between condominium and rental apartment can be a good way. Accommodating middle class in mixed-housing complex is considered to reduce the social gap. Socially, multilateral and continuous effort through government, industry and academic area is required to inspire positive awareness on social mix among residents in mixed housing complex.

• 대한안전경영과학회지
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• 제14권2호
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• pp.49-54
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• 2012

As all kind of industry has developed, metal structure and machine instrument use bolt, pin, rivet and welding for assembly and combination. For pin and hinge, dimension accuracy is crucial to keep the operation and safety of the structure and machine instrument. In case of complex machine, the hinge for cover open-loop system is one of the significant design elements. Most of the hinges are being imported and assembled sine they give high technology development cost for its unit cost position. The reason is that the localization of hinge is inadequate. As the demand increase and the necessity of localization grow, it is now more important than ever to develop low cost structure. By the low cost structure, a new technology could be obtained for electronic product and structural hinge since it would enable for complex machine hinge to be guaranteed, technologically. Open-loop hinge is the link type and designed for the structure to keep constant open-loop. And, the hinge is examined in design stability by finite element analysis method. In this paper, the operation result is presented when the hinge for complex machine open-loop is designed for link type structure.

• Asian Journal of Atmospheric Environment
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• 제7권1호
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• pp.56-63
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• 2013

Children are particularly vulnerable to adverse health effects associated with heavy metal exposure. The goal of this study was to investigate the relationship between proximity to an industry complex and blood lead and urinary cadmium levels for children aged 7-13 who lived in Ulsan where a big petrochemical complex is located. We conducted a questionnaire survey to collect data including sociodemographics, daily habits, residential environment, etc. We also analyzed blood lead and urinary cadmium levels using Atomic Absorption Spectrometry (AAS). Data were analyzed using regression analysis. All statistical analyses were conducted with SAS software version 9.2. We calculated distance by using a Geographic Information System (ArcGIS version 10.0). The geometric mean blood lead level was 1.55 ${\mu}g/dL$ (boys: 1.59 ${\mu}g/dL$, girls: 1.51 ${\mu}g/dL$), and the geometric mean urinary cadmium level was 0.51 ${\mu}g/g$ creatinine (boys: 0.45 ${\mu}g/g$ creatinine, girls: 0.58 ${\mu}g/g$ creatinine). In the results of regression analyses, we found that urinary cadmium levels significantly decreased as distance between residence and industrial complex increased after adjusting for age, gender, income, passive smoking and the length of residence. This result was opposite to that for lead levels. Our observations support the hypothesis that urinary cadmium levels in children are related to their proximity to an industrial complex.

• 한국산업융합학회 논문집
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• 제4권3호
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• pp.319-327
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• 2001

The objective of this study is to clarify the effect of thermal radiation environments on human thermal comfort, depending on different canyon types and surface materials on the human thermal comfort in a housing complex in Pohang city, Korea. For this purpose, the operative temperature and new effective temperature were calculated based on the modified mean radiant temperature of canyon models variated by the existence of direct radiation existence, surface materials, and the width and length of the street spaces in a housing complex. These indices for the canyon have been calculated from the meteorological data of Pohang city, which include air temperature, relative humidity, air velocity, global solar radiation and cloud. And the monthly averages of these climate factors measured at noon have been used. The results are as follows: (1) It is revealed that the short-wave radiosity reached the human body is affected by direct solar radiation and surface materials, and the long-wave radiosity by canyon types. (2) The existence of direct solar radiation, the kinds of surface materials and canyon types affect operative temperature($OT_n$) and new effective temperature($ET^*{_n}$). (3) The analysis of the human heat balance in the canyon indicates that the influence of radiation on human body is marc likely to be affected by the existence of direct solar radiation on human model.

• 한국지역지리학회지
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• 제18권3호
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• pp.283-297
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• 2012

본 연구의 목적은 클러스터 생애주기 분석을 기반으로 서울디지털산업단지의 진화 경로와 역동성을 분석하는 것이다. 1960년대 중반부터 1990년대 후반까지 업체수와 고용자수 변화를 중심으로 구로공단의 발생-성장-유지-쇠퇴의 생애주기를 구분하고 시기별 특성을 분석하였다. 1990년대 후반 이후 업체수와 고용자수가 급증하였고, 소규모 기술집약 중소기업과 지식기반 서비스기업이 서울디지털산업단지의 성장과 구조재편을 주도하였다. 이것은 지식산업센터(아파트형공장) 증가로 가능했으며, 2000년대 중반 이후에는 지식기반 클러스터로의 변환의 생애주기 모습을 보여준다.