• 제목/요약/키워드: colony energy

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Modification of Gamma-radiation Response in Mice by Green Tea and Diethyldithiocarbamate (마우스에서 방사선 영향에 대한 녹차와 Diethyldithiocarbamate의 조절효과)

  • Kim, Se-Ra;Kim, Sung-Ho;Lee, Hae-June;Oh, Heon;Ryu, Si-Yun;Lee, Yun-Sil;Kim, Tae-Hwan;Jo, Sung-Kee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.7
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    • pp.1108-1113
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    • 2003
  • We performed this study to determine the effect of green tea on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of gammairradiation. The radioprotective effect of green tea was compared with the effect of diethyldithiocarbamate (DDC). Jejunal crypts were protected by pretreatment of green tea (p<0.01). Green tea administration before irradiation resulted in an increase of the formation of endogenous spleen colony (p<0.05). The frequency of radiation-induced apoptosis in intestinal crypt cells was also reduced by pretreatment of green tea (p<0.05). The radioprotective effect on jejunal crypts and apoptosis in the DDC treated group appeared similar to those in the green tea treated groups. Treatment with DDC showed no significant modifying effects on the formation of endogenous spleen colony. These results indicated that green tea might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of green tea and its components.

Stimulation of Macrophage Differentiation by Bu-Zhong-Yi-Qi- Tang Extract (보중익기탕 추출물의 대식세포 분화 유도 효과)

  • Kang, Hye-In;Shin, Sung-Hae;Cho, Young-Sook;Cho, Sung-Ki;Byun, Mung-Woo;Yee, Sung-Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.3
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    • pp.330-335
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    • 2005
  • We have investigated the effects of Bu-Zhong-Yi-Qi- ang on the differentiation of murine bone marrow cells in methylcellulose culture. GM-CSF and IL-3 supported primarily the formation of granulocyte/mac-rophage colony formation. However, the addition of Bu-Zhong-Yi-Qi-Tang extract yielded a significant increase in the numbers of colonies and differentiated cells in the presence of GM-CSF and IL-3. We have analyzed CD11b (Mac-1) expression of differentiated cells from bone marrow by staining with monoclonal anti-CD11b antibody. The majority of colony-forming cells were in CD11b/sup +/ population. Also Bu-Zhong-Yi-Qi-Tang extract promoted the production of IL-6 and nitric oxide by macrophages. These results demonstrate that extract of Bu-Zhong-Yi-Qi-Tang, a prescription of traditional oriental medicine, is effective in supporting macrophage potential of the primary colonies.

Development of a Qualitative Dose Indicator for Gamma Radiation Using Lyophilized Deinococcus

  • Lim, Sangyong;Song, Dusup;Joe, Minho;Kim, Dongho
    • Journal of Microbiology and Biotechnology
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    • v.22 no.9
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    • pp.1296-1300
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    • 2012
  • The feasibility of using Deinococcus showing strong resistance to both desiccation and ionizing radiation as a dose indicator of gamma radiation exposure was evaluated. Three Deinococcus strains having different levels of radiation resistance, Deinococcus radiodurans (DRD), Deinococcus radiopugnans (DRP), and the DRD pprI mutant (DRM), were selected to develop an appropriate dose indicator for a broad range of exposures. DRD, DRP, and DRM cultures with different numbers of cells [${\sim}10^7$ to $10^3$ colony forming units (CFU)/$100{\mu}l$] were lyophilized and subjected to various doses of gamma radiation to determine a critical dose that inhibited bacterial growth completely. Finally, a combination of DRD at ${\sim}10^7$ and ${\sim}10^6$ CFU, DRP at ${\sim}10^5$ CFU, and DRM at ${\sim}10^4$ CFU successfully indicated exposure to 5, 10, 20, and 30 kGy of gamma radiation, respectively. This study shows the possibility of developing a qualitative indicator of radiation exposure using Deinococcus.

Identification of Radiation-Resistant Bacterium Isolated from Dried Laver (Porphyra tenera) (김으로부터 분리한 방사선 저항성 세균)

  • Ahn, Hyun-Joo;Yook, Hong-Sun;Kim, Dong-Ho;Kim, Sung;Byun, Myung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.1
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    • pp.193-195
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    • 2001
  • A radiation-resistant bacterium was isolated from gamma irradiated dried laver (Porphyra tenera) and its microbiological characteristics were examined. As a result of resistance test to gamma irradiation, the isolate was survived $10^{3}$ CFU/mL even at 30 kGy and significant shoulder line zone was shown until 20 kGy. The $D_{10}$ value was 11.27 kGy. The isolate was gram-positive, non-motile coccus and catalase-positive. n culture, the red-pigmented smooth colony was observed. The biochemical test in API (analytical profile index) system showed that the isolate fermented glucose and fructose as the carbon source. Therefore, a radiation-resistant bacterium isolated from laver was potentially identified as Micrococcus roseus sp.

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Isolation of Cesium and Radiation Resistance Bacteria for Bioremediation (생물정화를 위한 세슘 및 방사선 저항성 세균의 분리)

  • Jae Hoon Kim;Jai Hyunk Ryu;Sang Hoon Kim;Joon Woo Ahn;Soon Jae Kwon;Jin Baek Kim;Min Kyu Kim;Sang Young Im;Jae Nam Park
    • Journal of Radiation Industry
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    • v.17 no.2
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    • pp.183-190
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    • 2023
  • The global problem of handling radioactive materials is facing limitations. Eco-friendly bioremediation methods using microorganisms are being studied. This study was conducted to screen cesium-resistant microbial strains. M1 strain was selected from the soil sample by enriched culture in R2A medium containing 100 mM CsCl. In liquid medium containing above 40 mM of CsCl, the growth of M1 was inhibited in a concentration-dependent manner. Otherwise, M1 can survive up to 80mM CsCl in solid medium although the growth rate was slow and colony size was small. M1 strain was genetically identified as a strain of the genus Acinetobacter through 16S rRNA sequencing, and radiation resistance (D10 value) of M1 was found to be 0.307 kGy. These results showed that M1 strain is highly resistant to cesium and can grow in radiation environment. It was considered that M1 strain is useful in the field of biological decontamination of cesium.

Formation Estimation of Shaly Sandstone Reservoir using Joint Inversion from Well Logging Data (복합역산을 이용한 물리검층자료로부터의 셰일성 사암 저류층의 지층 평가)

  • Choi, Yeonjin;Chung, Woo-Keen;Ha, Jiho;Shin, Sung-ryul
    • Geophysics and Geophysical Exploration
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    • v.22 no.1
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    • pp.1-11
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    • 2019
  • Well logging technologies are used to measure the physical properties of reservoirs through boreholes. These technologies have been utilized to understand reservoir characteristics, such as porosity, fluid saturation, etc., using equations based on rock physics models. The analysis of well logs is performed by selecting a reliable rock physics model adequate for reservoir conditions or characteristics, comparing the results using the Archie's equation or simandoux method, and determining the most feasible reservoir properties. In this study, we developed a joint inversion algorithm to estimate physical properties in shaly sandstone reservoirs based on the pre-existing algorithm for sandstone reservoirs. For this purpose, we proposed a rock physics model with respect to shale volume, constructed the Jacobian matrix, and performed the sensitivity analysis for understanding the relationship between well-logging data and rock properties. The joint inversion algorithm was implemented by adopting the least-squares method using probabilistic approach. The developed algorithm was applied to the well-logging data obtained from the Colony gas sandstone reservoir. The results were compared with the simandox method and the joint inversion algorithms of sand stone reservoirs.

Development of an Apparatus for the Oviposition Induction of the Bumblebee Bombus ignitus Using Electricity (전기를 이용한 호박벌(Bombus ignitus)의 산란유도장치 개발)

  • Yoon, Hyung-Joo;Lim, Chae-Dae;Lee, Kyeong-Yong;Lee, Sang-Beom;Park, In-Gyun
    • Korean journal of applied entomology
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    • v.49 no.1
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    • pp.49-55
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    • 2010
  • Bumblebees are widely used to pollinate crops in greenhouses and fields. Here we firstly developed an apparatus for the oviposition induction of the bumblebee Bombus ignitus using electricity. The apparatus consists of boxes for colony initiation, part of temperature control, part of heat transfer, and moving shelf. The result shows that the rates of oviposition and colony foundation in the newly developed apparatus are respectively 3.9% and 5.2% higher than in the existing apparatus using hot water. More importantly, the newly developed apparatus is 75% cheaper in costs and can more save energy than existing apparatus. These results indicate that the newly developed apparatus could serve as an effective apparatus for the oviposition induction of B. ignitus.

Constrained Relay Node Deployment using an improved multi-objective Artificial Bee Colony in Wireless Sensor Networks

  • Yu, Wenjie;Li, Xunbo;Li, Xiang;Zeng, Zhi
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.6
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    • pp.2889-2909
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    • 2017
  • Wireless sensor networks (WSNs) have attracted lots of attention in recent years due to their potential for various applications. In this paper, we seek how to efficiently deploy relay nodes into traditional static WSNs with constrained locations, aiming to satisfy specific requirements of the industry, such as average energy consumption and average network reliability. This constrained relay node deployment problem (CRNDP) is known as NP-hard optimization problem in the literature. We consider addressing this multi-objective (MO) optimization problem with an improved Artificial Bee Colony (ABC) algorithm with a linear local search (MOABCLLS), which is an extension of an improved ABC and applies two strategies of MO optimization. In order to verify the effectiveness of the MOABCLLS, two versions of MO ABC, two additional standard genetic algorithms, NSGA-II and SPEA2, and two different MO trajectory algorithms are included for comparison. We employ these metaheuristics on a test data set obtained from the literature. For an in-depth analysis of the behavior of the MOABCLLS compared to traditional methodologies, a statistical procedure is utilized to analyze the results. After studying the results, it is concluded that constrained relay node deployment using the MOABCLLS outperforms the performance of the other algorithms, based on two MO quality metrics: hypervolume and coverage of two sets.

Evaluation on the radioprotective effect of Korean favorite teas (한국인 기호 차류의 방사선 장해 경감효과 평가)

  • Kim, Se-Ra;Lee, Hae-June;Oh, Heon;Lee, Jin-Hee;Kim, Hu-Kyung;Kim, Tae-Hwan;Jo, Sung-Kee;Kim, Sung-Ho
    • Korean Journal of Veterinary Research
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    • v.42 no.4
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    • pp.475-483
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    • 2002
  • We performed this study to determine the effect of Korean favorite teas (green tea, ginseng tea, coffee and barley tea) on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of ${\gamma}$-radiation. Jejunal crypts were protected by pretreatment of green tea (P.O.: 1.25% water extract, for 7 days before irradiation., I.P.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01) or ginseng (I.P.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.05). Green tea (p<0.05) or ginseng (p<0.05) administration before irradiation (I.P. at 12 and 36 hours before irradiation) resulted in an increase of t formation of endogenous spleen colony. The frequency of radiation-induced apoptosis was also reduced by pretreatment of green tea (P.O.: p<0.005, I.P.: p<0.05), pretreatment of ginseng (P.O.: p<0.005, I.P.: p<0.005) or posttreatment of ginseng (I.P.: 50 mg/kg of body weight, at 30 minutes after irradiation, p<0.05). Treatment with coffee or barley tea showed no significant modifying effects on the radiation-induced damages. These results indicated that green tea and ginseng might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of green tea, ginseng and its components.

Research on Bacterial Distribution of Public Lavatory (공중이용 화장실의 미생물 분포 조사)

  • Choi, Han-Young;Kwon, Woo-Taeg;Lee, Woo-Sik;Lee, Hyang
    • Journal of Environmental Health Sciences
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    • v.38 no.6
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    • pp.520-528
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    • 2012
  • Objective: This study was performed to assess bacterial distribution concerned in sanitation management of public lavatory in Seoul. Methods: In this research, bacteriological investigation on public lavatory was accomplished for bidet water, bidet nozzle, washbowl and lavatory stool in the 50 public facilities such as public institutions, subway stations, cinema, department stores, large-scale buildings and hospitals amount to 374 specimens. Results: The geometric mean of colony forming unit(CFU) in total aerobic colony count were analyzed as follows; $5.2{\times}10^2/100cm^2$ on lavatory stool, $7.2{\times}10^3/ea$ on bidet nozzle, $7.8{\times}10^3/ea$ on center ring of washbowl, $1.4{\times}10/mL$ in bidet water (ml) and 7.0/ea on doorknob. Opportunistically pathogenic germs such as Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa were isolated in 3.7%, 5.9%, 3.2% and 1.9% of total specimens, respectively. Conclusion: The result of this study shows that there were some facilities where the pathogenic germs were detected to may cause urological infection. And the CFU of general bacteria as the representative indicator of disinfection and lavatory cleaning were high enough to imply the improvement of sanitation management of public lavatories should be contrived.