• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.919-933
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• 2021

We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10% fetal bovine serum. When the cells reached 80%-90% confluence, we used the differentiation medium with 5% horse serum for differentiation for 96 h. The differentiation medium contained 50 µM, 100 µM and 200 µM POA. Control BSC were cultured only in differentiation media. Compared with the control BSC, the POA BSC significantly up-regulated the expression of paired box 3 (Pax3) and paired box 7 (Pax7) and down-regulated myogenin gene expression (p < 0.01), which indicates a depression in muscle fiber development. However, all POA treatments up-regulated the expression of the adipocyte transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha and beta (C/EBP α and C/EBP β), and other genes (p < 0.01) and increased the expression of PAT-family proteins and the concentration of adiponectin in the media. These results indicate that POA can convert part of BSCs into adipocytes.

• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.934-953
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• 2021

Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

• Journal of Ocean Engineering and Technology
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• v.11 no.3
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• pp.8-19
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• 1997

In this study an adequate type of offshore structure at the Sakhalin region as cold region is proposed and its structural design results are presented based on the reliability analysis. Structural safety assessment has been carried out for the proposed offshore structure at the Sakhalin area as designed by the reliability method. And a rational design procedure is presented based on the reliability analysis. Followings are drawn through the present study : - Four colum TLP structure is proposed as an adequate offshore structure type at the cold region like the Sakhalin region and the reliability-based structural design results are presented. It is seen that the proposed type is a more adequate and economic than the fixed type. - Safety assessment of the proposed structure applying the extended incremental load method is performed. - Referring the allowable safety level for offshore structures it has been found present TLP structure has sufficient structural safety at the system level as well as at the component level.

• Journal of the Korean Society for Heat Treatment
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• v.27 no.2
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• pp.59-64
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• 2014

Cu-Al-Mn shape memory alloys of a variety of composition were characterized in terms of shape memory properties and cold workability. Cold workability tested by cold rolling indicated that the alloys solution treated in the ${\alpha}+{\beta}$ region have a higher ductility than those solution treated in the ${\beta}$ region. Also it is known that cold workability increased with the decrease in Al content in the ${\beta}$ region. This seems to be resulted from the fact that Mn addition causes to expand ${\beta}$ region toward lower Al content and lower order-disorder transition temperature, consequently, ${\beta}$ of excellent workability being frozen even at room temperature. Experimental results regarding shape memory showed that the properties were better with a higher Al contents at a given Mn content, which is closely related with martensitic transformation. It is also shown that super elasticity limit was enhanced with decrease in the yield strength of alloys because a lower yield strength seems to initiates slip at the lower applied stress.

• Journal of Advanced Marine Engineering and Technology
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• v.27 no.3
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• pp.340-355
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• 2003

본 해설에서는 현재 및 앞으로도 그 중요성과 필요성이 점차적으로 강조되고 있는 재료들에 대한 특성들에 대해 해설하고자 하였다. 다양한 형태의 구조물에 사용되고 있으며. 그에 대한 연구와 개발이 끊임없이 진행되고 있는 각각의 재료들에 대한 저온과 같은 극한 환경 조건에서의 충분한 지식을 터득하고 그러한 재료들에 필요로 하는 특정한 물성에 대한 요구를 충족시키고 광범위하게 적용될 수 있는 시험법 등을 소개하고자 하였다.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.7 no.3
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• pp.382-395
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• 1995

A numerical study has been performed on flow characteristics in a domestic refrigerator whose size is $540mm{\times}1,530mm{\times}680mm$, considering existence of a fan and evaporator. The flow field has been simulated with the low Reynolds number $k-\bar{\varepsilon}$ turbulent model and SIMPLE algorithm based on the finite volume method. The region of fan which makes driving force for cold air distribution was modeled as a region in which momentum sources are generated uniformly. The concept of the distributed pressure resistance was applied to describe the momentum loss from evaporator. The result showed that the rate of cold air distribution into freezing room and cold storage room was almost 7 : 3.

• Journal of the Korean GEO-environmental Society
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• v.18 no.1
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• pp.49-58
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• 2017

Due to low annual average temperature, road tunnel lining in domestic cold region (Gangwon province) experiences durability problems. The financial and human damage due to cracks, breakout, exfoliation and water leakage increases every year. However, domestic research on effect of temperature on road tunnel lining damage is insufficient. Thus, this research has investigated 70 tunnels located in cold region (Gangwon-do) to analyze damage status. Furthermore, by contrasting damage on tunnels in relatively warm Gangneung area with those in relatively cold Hongcheon area, the effect of temperature on road tunnel lining damage was analyzed.

• Journal of the Korean Recycled Construction Resources Institute
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• v.5 no.4
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• pp.466-473
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• 2017

RC (Reinforced Concrete) member has varying service life due to varying diffusion characteristics with loading conditions even if it is exposed to constant exterior conditions. In the paper, quantitative parameters are obtained through adopting the previous results for effects of compressive, tensile, and cold joint on chloride diffusion in OPC (Ordinary Portland Cement) and GGBFS (Ground Granulated Blast Furnace Slag) concrete. Service life is evaluated in RC simple beam with 10.0m of span through increasing loading from self weight (2.5kN/m) to the loading to cracking moment (5.5kN/m). In OPC concrete without cold joint, service life changes to 89.4% for tensile region and 101% for compressive region with loadings while GGBFS concrete has 80.0% and 106%, respectively. For cold joint area, GGBFS concrete shows much reduced service life to 82~80% in compressive region and 69~61% in tensile region, which is caused by the lower diffusion in normal condition but relatively higher increasing cold joint effect than OPC concrete.

• Proceedings of the Korea Concrete Institute Conference
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• 1994.10a
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• pp.200-204
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• 1994

Low temperature associated damage mechanism is not well known for asphalt concrete. Many studies have related the thermal cracking of pavement in the roadway in cold region with overall shrinkage of the pavement surface under assumption of homogeneous material. This study, however, was intiated based on the assumption that thermal incompatibility of materials (heterogeneous) in asphalt concrete mixture would be the primary cause of the damages. Acoustic emission technique and microscopic obsevation were employed to evaluate damage mechanism of asphalt concrete due to low temperature. The first method showed the sufficient evidence that asphalt concrete could be damaged by lowered temperature only. The second method showed that the damage by temperature resulted in micro-cracks at the interface between asphalt matrix and aggregate particle. It was concluded that these damage mechanisms were the primary cause of major thermal cracking of asphalt pavement in cold region.

• Applied Chemistry for Engineering
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• v.28 no.4
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• pp.460-466
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• 2017

The common petroleum can make precipitation such as a wax in a polar region due to severely cold weather condition, which can cause problems to fuel supply system. The petroleum product used in the polar region has thus been manufactured and sold suitable for the cold environment. However it is difficult to supply such petroleum products on time since these were mainly supplied abroad. In this article, the original fuel properties were first analyzed in order to find alternative fuel products for polar region. Jet fuel which is excellent cold characteristics was chosen and the fuel properties was tested by adding a constant concentration of lubricant additives to the jet fuel. As a result, the lubricant additive R621 showed the best lubricity, and adding 1000 mg/L of R621 content to the jet fuel was sufficient to enhance the lubricity. We envision that the jet fuel added 1000 mg/L of R621A can be suitable for alternative special antarctic blend diesel (SAB) in an severe polar environment.