• 한국표면공학회지
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• 제36권5호
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• pp.379-385
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• 2003

The surface morphology, the glossiness and the hardness of Zn-Cr and Zn-Cr-X(X:Co, Mn) alloy electrodeposits were investigated by using chloride bath with EDTA additive and flow cell system. The surface morphology of Zn-Cr alloy and Zn-Cr-Mn alloy changed from fine needle shape crystalline structure to colony structure of fine granular crystallites with increasing current density in the range of 20-100 $A/dm^2$. The surface morphology of Zn-Cr-Co alloy deposited from low Co concentration bath(2.5-10 g/$\ell$) was similar to that of Zn-Cr alloy, while that of Zn-Cr-Co alloy deposited from high cobalt concentration bath was fine granular crystalline structure in the same range of current density. The glossiness of Zn-Cr and Zn-Cr-Mn alloy increased noticeably with increasing current density, while that of Zn-Cr-Mn alloy decreased with increasing Mn concentration of bath in high current density region. The glossiness of Zn-Cr-Co alloy deposited from low Co concentration bath increased with current density while that of the alloy from high Co concentration bath decreased with increasing current density. The hardness of Zn-Cr and Zn-Cr-X alloy increased noticeably with current density.

• 한국동물학회지
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• 제34권2호
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• pp.142-147
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• 1991

드렁허리의 혈장에서 얻은 angiotensin I-converting enzyme(ACE)의 활성도를 포유동물과 비교하였다. 드렁허리의 혈장내 ACE는 pH 10에서 가장 높은 활성도를 나타냈으며 포유동물에서 보다 알칼리성이었다. 또한 최적활성도를 나타내는 데 필요한 C1 이온의 요구성은 종에 따라 다르게 나타났다. 드렁허리의 ACE 활성도는 ACE의 활성억제제인 EDTA, teprotide 및 captopril에 의해서 이들의 농도에 따라 억제 되었으며, 이 억제현상은 포유동물의 혈장 ACE와 매우 비슷하였다. 드렁허리의 ACE 활성도는 cobalt에 의하여 증가되었으며, 포유동물에 비해서 열에 불안정하였다. 또한 여러 조직중에서느 뇌에서 가장 높은 ACE 활성도가 측정되었다.

• Bulletin of the Korean Chemical Society
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• 제9권1호
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• pp.1-4
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• 1988

Rates of solvolysis of trans-[Co$(N-eten)_2Cl_2$]$^+$ have been investigated using spectrophotometric method at various pressures and temperatures in the mixtures of water with the t-butyl alcohol which possesses a high structure inducing capacity in water. The values of ${\Delta}V^{\neq}$ obtained from pressure effect on the rate constants were 2.55∼ 5.83 $cm^3mol^{-1}$. These values were discussed in terms of dissociative mechanism. Extrema found in the variation of ${\Delta}H^{\neq}$ and ${\Delta}S^{\neq}$ with solvent composition correlated with extrema in the variation of the physical properties of the mixtures. The logarithms of rate constants correlated linearly with both Grunwald-Winstein parameter and the reciprocal of dielectric constant ($Ds^{-1}$). The gradient, m of Grunwald-Winstein plot for the trans-[Co$(N-eten)_2Cl_2$]$^+$ was 0.09, which is significantly lower than those for the other cobalt (Ⅲ)-dichloro complexes. It was suggested that the reaction is an Id mechanism with long extension of Co-Cl bond in the transition state, as found for the C-Cl bond in the transition state for the solvolysis of t-butyl chloride.

• Bulletin of the Korean Chemical Society
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• 제11권4호
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• pp.309-313
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• 1990

The rates of solvolysis of trans-$[Co(N-eten)_2Cl_2)$+ (N-eten; N-ethylethylenediamine) have been investigated using spectrophotometric method in binary aqueous mixtures containing methyl alcohol, isopropyl alcohol, t-butyl alcohol, ethylene glycol and glycerol. The values of ${\Delta}H^{\neq}$ and ${\Delta}S^{\neq}$ obtained from temperature effect on the rate constants were $80{\sim}84 kJmol^{-1}$ and $- 28{\sim} - 45 JK^{-1}mol^{-1}.$ Extrema found in the variation of the enthalpy and entropy of activation with solvent composition correlated very well with extrema in the variation of the physical properties of mixture which relate to sharp change in the solvent structure. The reaction mechanism was discussed in terms of correlation diagrams involving the exess molar Gibbs function of mixing for the binary mixtures. The behavior of this cobalt(Ⅲ) complex was compared with that of t-butyl chloride. The application of free energy cycle to the process initial state to transition state in water and in the mixture showed that the solvation of transition state had dominant effect on the rates in the mixtures. It was found that $S_N1$ character was increased with increasing the content of co-solvent in the mixture.

• 생명과학회지
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• 제20권12호
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• pp.1820-1828
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• 2010

PC12 세포에서 $CoCl_2$에 의한 hypoxia 유도는 HIF1 alpha의 상승 발현으로 확인하였다. 이때 apoptosis의 유도는 genomic DNA의 fragmentation과 apoptotic body는 Hoechst 염색으로 확인되었고, ER luminal chaperone의 발현 및 ER stress signal에 관여하는 ER membrane kinase인 IRE1, PERK, ATF6의 발현도 확인되었다. 이들이 apoptosis로 연결되는 고리 역할을 하는 IRE1-XBP1 mRNA splicing, PERK-eIF2 alpha, ATF6 protein cleavage도 반응하는 것으로 확인되었다. 위의 결과는 신경세포의 hypoxia상태는 ER stress signal pathway를 거쳐서 apoptosis가 된다는 것을 증명한 것으로 신경세포의 hypoxia치료를 위한 기초 자료가 될 것으로 생각한다.

• 자원리싸이클링
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• 제32권1호
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• pp.21-32
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• 2023

리튬이온 배터리(LIB) 제조를 위한 리튬의 사용이 점차 증가함에 따라 그에 따라 발생되는 리튬이온배터리 폐기가 증가될 것으로 사료된다. 이에 따라 폐배터리를 재활용을 하기위한 용매 추출을 통한 재활용에 대한 활발한 연구가 니켈, 코발트 및 망간과 같은 유가금속을 제거한 후 얻은 폐 용액에서 리튬의 회수가 중요하다. 본 연구에서는 폐이차전지 재활용공정 후 발생되는 폐액에서 리튬을 회수하기위해 추출제 Di-(2-ethylhexyl) hosphoricacid(D2EHPA)와 등유의 개질제 Tri-n-butyphosphate(TBP)를 선택적으로 혼합하여 추출조건을 최적화하였다. 폐액에는 리튬과 고농도의 나트륨(Li⁺ = 0.5% ~ 1%, Na⁺ = 3 ~ 6.5%)을 함유하고 있었으며, 리튬의 추출은 유기용매의 다른 구성에서 최종적으로 20% D2EHPA + 20% TBP + 60% 등유로 구성된 유기용매에서 효과적인 추출을 조건을 확립하였다. NaOH의 비누화를 이용한 SX 시스템에서는 평형 pH 4~4.5에서 유기 대 수성(O/A)이 5일 때 약 95% 이상의 리튬이 선택적으로 추출되는 것을 확인하였다. 적은 양의 나트륨으로 염화리튬에서 탄산리튬 분말을 얻기 위해 고순도 중탄산암모늄을 처리하였다. 최종적으로 처리된 탄산리튬에 여러번 세수를 통하여 미량의 나트륨을 제거하고 고순도 탄산리튬 분말(순도 99.2%)을 제조하였다. 따라서 본 연구를 통하여 폐이차전지 재활용공정에서 발생되는 폐액을 활용하여 탄산리튬의 효율적인 제조방법을 확인하였다.

• Journal of Nutrition and Health
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• 제48권5호
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• pp.451-456
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• 2015

Purpose: Neuronal apoptotic events induced by aging and hypoxic/ischemic conditions is an important risk factor in neurodegenerative diseases such as ischemia stroke and Alzheimer's disease. The peel of Citrus sunki Hort. ex Tanaka has long been used as a traditional medicine, based on multiple biological activities including anti-oxidant, anti-inflammation, and anti-obesity. In the current study, we examined the actions of fermented C. sunki peel extract against cobalt chloride ($CoCl_2$)-mediated hypoxic death in human neuroblastoma SH-SY5Y cells. Methods: Cell viability was measured by trypan blue exclusion. Expression of apoptosis related proteins and release of cytochrome c were detected by western blot. Production of intracellular reactive oxygen species (ROS) and apoptotic morphology were examined using 2',7'-dichlorofluorescin diacetate (DCF-DA) and 4',6-diamidino-2-phenylindole (DAPI) staining. Results: Exposure to $CoCl_2$, a well-known mimetic agent of hypoxic/ischemic condition, resulted in neuronal cell death via caspase-3 dependent pathway. Extract of fermented C. sunki peel significantly rescued the $CoCl_2$-induced neuronal toxicity with the cell viability and appearance of apoptotic morphology. Cytoprotection with fermented C. sunki peel extract was associated with a decrease in activities of caspase-3 and cleavage of poly (ADP ribose) polymerase (PARP). In addition, increase in the intracellular ROS and release of cytochrome c from mitochondria to the cytosol were inhibited by treatment with extract of fermented C. sunki peel. Conclusion: Based on these data, fermented C. sunki peel extract might have a protective effect against $CoCl_2$-induced neuronal injury partly through generation of ROS and effectors involved in mitochondrial mediated apoptosis.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.43-47
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• 2008

The nickel and cobalt resistance of Cupriavidus metallidurans CH34 is mediated by the CnrCBA efflux pump encoded by the cnrYHXCBAT metal resistance determinant. The products of the three genes cnrYXH transcriptionally regulate expression of cnr. CnrY and CnrX are membrane-bound proteins, probably functioning as anti-sigma factors, whereas CnrH is a cnr-specific extracytoplasmic functions (ECF) sigma factor. The periplasmic domain of CnrX (residues 29-148) was cloned as a N-terminal His-tagged protein, expressed in Escherichia coli, and purified using affinity chromatography and gel filtration. The molecular mass was estimated to be about 13.6kDa by size exclusion chromatography, corresponding to a monomer. The tetragonal bipyramid crystals were obtained by mixing an equal volume of protein in 50mM Tris-HCl, pH 7.5, 1% glycerol, 100mM NaCl, 1mM DTT, and the reservoir solution of 15% w/v PEG 2000, 100mM lithium chloride at 277K in 2-4 days using hanging drop vapor diffusion. The protein concentration was 24mg/ml. The crystal that diffracted to $2.42{\AA}$ resolution belongs to space group $P4_1\;or\;P4_3$ with unit cell parameters of $a=b=32.14{\AA},\;c=195.31{\AA},\;{\alpha}={\beta}={\gamma}=90^{\circ}$, with one molecule of CnrX in the asymmetric unit.

• International Journal of Oral Biology
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• 제38권3호
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• pp.111-119
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• 2013

Objective. To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation-mimicking agent cobalt chloride ($CoCl_2$) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying molecular mechanisms. Study design. The dose and exposure periods for $CoCl_2$ in hMSCs were optimized by cell viability assays. After confirmation of $CoCl_2$-induced HIF-$1{\alpha}$ and vascular endothelial growth factor expression in these cells by RT-PCR, the effects of temporary preconditioning with $CoCl_2$ on hMSC osteogenic differentiation were evaluated by RT-PCR analysis of osteogenic gene expression, an alkaline phosphatase (ALP) activity assay and by alizarin red S staining. Results. Variable $CoCl_2$ dosages (up to $500{\mu}M$) and exposure times (up to 7 days) on hMSC had little effect on hMSC survival. After $CoCl_2$ treatment of hMSCs at $100{\mu}M$ for 24 or 48 hours, followed by culture in osteogenic differentiating media, several osteogenic markers such as Runx-2, osteocalcin and osteopontin, bone sialoprotein mRNA expression level were found to be up-regulated. Moreover, ALP activity was increased in these treated cells in which an accelerated osteogenic capacity was also verified by alizarin red S staining. Conclusions. The osteogenic differentiation potential of hMSCs could be preserved and even enhanced by $CoCl_2$ treatment.

• 한국환경보건학회지
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• 제10권2호
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• pp.41-51
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• 1984

This study was performed using samples collected at Myungryundong and at Reservoirs. The purpose of this study was to investigate the differences of water quality between tap and raw water, and to analyse drinking water quality by Fe, Zn from corroded galvanized steel pipe. Results were as follows 1. The older the pipe was, the higher the concentration of Ferrum and Zinc was (t-test : p<0.05). Ferrum and Zinc also exceeded the limits in the older galvanized steel pipe. I think that this comes from the corrosion of pipe. 2. Mercury, Arsenic, Cadmium, Lead, Chomium, Argentum and Aurum not detected in raw water were not detected in tap water. Cobalt, Bismuth and Molybudenum detected in raw water were not detected in tap water. I think that this comes from the quality of raw water, the result of water treatment and the improbability of detection of above metals in water delivery system. 3. Silicon measured 2.4698ppm in raw water, but it ranged from 0.4769ppm to 1.982 ppm in tap water. Manganese measured 0.0638ppm in raw water, but it ranged from 0.0026ppm to 0.0198ppm in 17cases(31%) out of 55samples in tap water. I think that this comes from the water treatment. 4. Aluminium not detected in raw water was found in 17 cases (31%) out of the samples (55cases). It may be considered as the use of coagulants $Al_2(SO_4)_3$. $18H_2O$ and PAC (Poly Aluminium Chloride). The concentration of copper in tap water was much higher in 2 cases(3.6%) out of the samples(55) than that of copper in raw water. I think that this may come from the use of ${CuSO}_4$, the preventive of algae growth, and the result of chlorination, but further study must be necoessary to support the proof.