• Journal of Life Science
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• v.22 no.6
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• pp.837-843
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• 2012

This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the $1^{st}$ cultivation experiment, and selected six strains from one of 58 strains through the $2^{nd}$ cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of ${\alpha}$-amylase at the highest as 73.9~102.2 unit/mg protein, and chitinase is lower than ${\alpha}$-amylase at 8.1~13.1 unit/mg protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of ${\alpha}$-amylase is the highest at 5,292~1,184 unit/mg protein, and CMCase and xylanase were 1,140~245 unit/mg protein, 94~575 unit/mg protein, compared to each other. However, the enzyme activity of ${\beta}$-glucosidase and chitinase is low.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.4
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• pp.216-222
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• 2002

Field experiments were conducted to investigate the effect of various cultural practices on methane($CH_4$) emission in tillage and no-tillage practice in a clayey paddy soil from 1998 to 2000. The factors evaluated in tillage and no-tillage methods were types of nitrogen fertilizers, application method of chemical fertilizers, rice straw application and cultivation method. Of the nitrogen fertilizers, the amount of $CH_4$ emission in ammonium sulfate plot was the lowest, regardless of tillage and the application method. 26.4~41.1% of reduction by ammonium sulfate compared with urea. But in no-tillage which have problem of poor rice yield than tillage, coated urea was more effective nitrogen fertilizer because that showed similar $CH_4$ emission and highest rice yield at 80% of dosage of nitrogen. No-tillage plot emitted lower $CH_4$ than tillage plot where the fertilizers were incorporated. On the contrary, no-tillage plot showed a little higher $CH_4$ emission compared with tillage plot for the surface application. When rice straw was applied, no-tillage practice reduced methane emission by 26.6% compared with tillage practice, but showing a little difference of 10.7% in no application. With cultivation method, no-tillage practice reduced methane emission 26.6% compared with tillage for the 30-d-old seedling transplanting. But for the dry direct seeding practice, no-tillage was a less effective because considerable amounts of rice straw incorporated by tillage were more decomposed aerobically in the soil and emitted as $CO_2$ to the atmosphere with flooding in no-tillage soil.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.429-440
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• 2021

During the growing period, the integrated solar radiation inside the greenhouse was 12.7MJ·m^-2d^-1, and which was 90% of the average daily global radiation outside the greenhouse, 14.1MJ·m^-2d^-1. The 24-hour average temperature inside the greenhouse from July to August, which has the highest temperature of the year, was 3.04℃ lower than the outside temperature, and 4.07℃ lower after the rainy season. Before the operation of fog cooling system, the average daily RH (%) was lowered to a minimum of 40% (20% for daytime), making it inappropriate for paprika cultivation, but after the operation of fog system, the daily RH during the daytime increased to 70 to 85%. The average humidity deficit increased to a maximum of 12.7g/m³ before fog supply, but decreased to 3.7g/m³ between July and August after fog supply, and increased again after October. The daytime residual CO₂ concentration inside the greenhouse was 707 ppm on average during the whole growing period. The marketable yield of paprika harvested from July 27th to November 23rd, 2020 was higher in 'DSP-7054' and 'Allrounder' with 14,255kg/10a and 14,161kg/10a, respectively, followed by 'K-Gloria orange', 'Volante' and 'Nagono'. There were significant differences between paprika cultivars in fruit length, fruit diameter, soluble solids (°Brix), and flash thickness (mm) of paprika produced in summer season at large single-span plastic greenhouse. The soluble solids content was higher in the orange cultivars 'DSP-7054' and 'Naarangi' and the flesh thickness was higher in the yellow and orange cultivars, with 'K-Gloria orange' and 'Allrounder' being the thickest. The marketable yield of paprika, which was treated with cooling and heating treatments in the root zone, increased by 16.1% in the entire cultivars compared to the untreated ones, increased by 16.5% in 'Nagano', 10.3% in the 'Allrounder', 20.2% in the 'Naarangi', and 17.3% in 'Raon red'.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.217-227
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• 2006

Micronutrient status in soils and crops of plastic film house and their relationship were investigated. Total 203 plastic film houses were selected (red pepper, 66; cucumber, 63; tomato, 74) in Yeongnam region and soil and leaf samples were collected. Hot-water extractable B and 0.1 N HCl extractable Cu, Zn, Fe, and Mn in soil samples and total micronutrients in leaf samples were analyzed. Contents Zn, Fe, and Mn in most of the investigated soils were higher than the upper limits of optimum level for general crop cultivation. Contents of Cu in most soils of cucumber and tomato cultivation were higher than the upper limit of optimum level, but Cu contents in about 30% of red pepper cultivation soils were below the sufficient level. Contents of B in most soils of cucumber and tomato were above the sufficient level but in 48% of red pepper cultivation soils B were found to be deficient. Micronutrient contents in leaf of investigated crops were much variable. Contents of B, Fe, and Mn were mostly within the sufficient levels, while in 71% of red pepper samples Cu was under deficient level and in 44% of cucumber samples Cu contents were higher than the upper limit of sufficient level. Contents of Zn in red pepper and cucumber samples were mostly within the sufficient level but in 62% of tomato samples Zn contents were under deficient condition. However, any visible deficiency or toxicity symptoms of micronutrients were not found in the crops. No consistent relationships were found between micronutrient contents in soil and leaf, and this indicates that growth and absorption activity of root and interactions among the nutrients in soil might be important factors in overall micronutrient uptake of crops. For best management of micronutrients in plastic film house, much attention should be focused on the management of soil and plant characteristics which control the micronutrient uptake of crops.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.318-329
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• 2014

In order to gain insight into the physiological responses of plants to high temperature stress, the effects of temperature on Chinese cabbage (Brassica campestris subsp. napus var. pekinensis cv. Detong) were investigated through analyses of photosynthesis and chlorophyll fluorescence under 3 different temperatures in the temperature gradient tunnel. Growth (leaf length and number of leaves) during the rosette stage was greater at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures than at ambient temperature. Photosynthetic $CO_2$ fixation rates of Chinese cabbage grown under the different temperatures did not differ significantly. However, dark respiration rate was significantly higher in the cabbage that developed under ambient temperature relative to elevated temperature. Furthermore, elevated growth temperature increased transpiration rate and stomatal conductance resulting in an overall decrease of water use efficiency. The chlorophyll a fluorescence transient was also considerably affected by high temperature stress; the fluorescence yield $F_J$, $F_I$, and $F_P$ decreased considerably at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, with induction of $F_K$ and decrease of $F_V/F_O$. The values of RC/CS, ABS/CS, TRo/CS, and ETo/CS decreased considerably, while DIo/CS increased with increased growth temperature. The symptoms of soft-rot disease were observed in the inner part of the cabbage heads after 7, 9, and/or 10 weeks of cultivation at ambient $+4^{\circ}C$ and ambient $+7^{\circ}C$ temperatures, but not in the cabbage heads growing at ambient temperature. These results show that Chinese cabbage could be negatively affected by high temperature under a future climate change scenario. Therefore, to maintain the high productivity and quality of Chinese cabbage, it may be necessary to develop new high temperature tolerant cultivars or to markedly improve cropping systems. In addition, it would be possible to use the non-invasive fluorescence parameters $F_O$, $F_V/F_M$, and $F_V/F_O$, as well as $F_K$, $M_O$, $S_M$, RC/CS, ETo/CS, $PI_{abs}$, and $SFI_{abs}$ (which were selected in this study), to quantitatively determine the physiological status of plants in response to high temperature stresses.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.56-63
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• 2014

Using commercial radish varieties for processing, about 30% of radish was discarded due to the root shape and low purity. To raise the processing ability, we tried to develop a new variety producing H-shaped root. As another characteristic required in variety for processing is high purity, we tried to raise purity using simple sequence repeats (SSR) markers for testing seed purity in every segregating generation. To develop Male-sterile (MS) seeding parent, we crossed commercial variety of 'Gwan dong spring' and 'Gyeo ryong spring'. One elite inbred was selected as recurrent parent for the MS plant. The major horticultural traits of selected inbred line were disease resistance, late bolting, heat resistance and bright green root top color. To develop pollen parent, we crossed commercial variety of 'Tae sang king' and 'Seoul spring'. We used individual selection method to develop H-shaped hard root and disease resistant inbred. In each segregating generation, we selected one plant based on phenotype and the uniformity of selected plant was tested by SSR markers using self-pollinated seeds. In the first segregating generation, 64.6% of sib plants shared the same band in PCR amplification using ACMP-490 primer and 66.7% using cnu-316 primer. The uniformity of segregating generations using ACMP-490 and cnu-316 raised in second generation to 68.8%, 70.8%, respectively; in third generation to 93.8%, 100%; in fourth generation to 93.8%, 100%; in fifth generation to 95.8%, 100%; in sixth generation to 100%, 100%. A novel cross was made using selected MS parent and pollen parent. When we checker the horticultural traits using autumn cultivation, the novel cross variety produced H-shaped root comparing other commercial varieties and produced highly uniform radish. Thus we registered this novel cross variety as 'YR ORE' at 2013 (Registration No. 4550).

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.151-157
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• 2003

Obtaining a sufficient amount of healthy keratinocytes from a small tissue is difficult. However, ORS cells can be a good source of epithelium since they are easily obtainable and patients do not have to suffer from scar formation at donor sites. Accordingly, the current study modified the conventional primary culture technique to overcome the low propagation and easy aging of epithelial cells during culturing. In a conventional primary culture, the average yield of human ORS tells is 2.↑ $\times$ 10$^3$cells/follicle based on direct incubation in a trypsin (0.1%)/EDTA(0.02%) solution for 15 min at 37$^{\circ}C$, however, our modified method was able to obtain about 6.9 $\times$ 10$^3$cel1s/follicle using a two-step enzyme digestion method involving dispase (1.2 U/mL) and a trypsin (0.1%)/EDTA (0.02%) solution. Thus, the yield of primary cultured ORS cells could be increasd three times higher. Furthermore, a total of 2.0 $\times$ 10$^{7}$ cells was obtained in a serum-free medium. while a modified E-medium with mitomycin C-treated feeder tells produced a total of 6.3 $\times$ 10$^{7}$ Cel1s over 17 days When Starting With 7.5 $\times$ 10$^4$cells. Finally, We Confirmed the effectiveness of our ORS tell isolation method by presenting their ability for reconstructing the bioartificial skin epithelium in vitro

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.193-198
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• 2006

Effects of acetosyringone and on Agrobacterium-mediated transformation of orchardgrass were investigated. Embryogenic calli induced from 3 varieties, Frontier, Potomac and Roughrider, were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase II(NPTII) and intron-containing ${\beta}-glucuronidase$ (intron-GUS) genes in the T-DNA region. The effects of varieties and acetosyringone(AS) concentrations on transformation and the expression of the GUS gene were investigated. Inclusion of $200{\mu}M$ AS in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of orchardgrass.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.12
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• pp.2082-2087
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• 2013

The present study was performed to quantitatively analyze eleutherosides (B and E) and ${\beta}$-glucan in different plant parts of three cultivars (Chungnam, Gangwon, and Jeju) of Acanthopanax senticosus and Acanthopanax koreanum using HPLC and a commercial enzyme kit. Our results showed high linearity in the calibration curves as the coefficients of correlation ($R^2$) were 0.998 (eleutheroside B) and 0.999 (eleutheroside E), respectively. Eleutheroside B and E were found in stem extracts of A. koreanum cultivated in Jeju (1,122 ${\mu}g/g$, eleutheroside B) and A. senticosus cultivated in Chungnam (2,536 ${\mu}g/g$, eleutheroside E), respectively. However, eleutheroside B was not detected in any part of A. senticosus cultivated in Chungnam. For ${\beta}$-glucan contents, stems of A. senticosus and A. koreanum showed higher than other parts. Furthermore, the ${\beta}$-glucan content in stems of A. koreanum cultivated in Gangwon was significantly higher than in those of other cultivars. These results show that the contents of eleutheroside B, E, and ${\beta}$-glucan were higher in stem extracts of A. senticosus and A. koreanum than other parts. Moreover, our results suggest that the contents of eleutheroside B, E, and ${\beta}$-glucan in A. senticosus and A. koreanum are influenced by cultivation area and the selected part.

• Korean Journal of Food Science and Technology
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• v.5 no.2
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• pp.101-107
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• 1973

1) For the purpose of preparation of food and fodder yeasts from nonedible seaweed, two suitable Candida yeasts have been isolated from seaweed compost. 2) They had the ability of fermenting galactose, sucrose and glucose, and could not ferment maltose and mannit, but could assimilate mannit. 3) NaCl concentration from 1 to 2% had no remarkable effect on growth of yeast and the optimum pH was $4{\sim}5$. 4) In the acid hydrolyzate of brown seaweed (Ecklonia cava Kjellman, Sargassum fulvellum AGARDH) an amorphous deposit was produced during storage after neutralization of media and its removal always delayed yeast growth, but addition of $(NH_4)_2SO_4$ and $NaH_2PO_4$ to media could increase the assimilation of reducing sugar and yeast yield. 5) $Co^{60}$ gamma ray irradiation (dose rate : 1 Mrad/hr, BNL shipboard irradiator) of seaweed had not so much effect on the hydrolysis of carbohydrates and nitrogen compounds in seaweed but could increase the yeast production from seaweed hydrolyzate. 6) The yeast yield was $7{\sim}8$ g of dry yeast per 100 g of seaweed by cultivation with jar fermentor.