• Journal of Aquaculture
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• v.17 no.1
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• pp.69-80
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• 2004

The objective of the present study was to analyze genetic distances, variation and characteristics of individuals in rainbow trout, Oncorhynchus mykis using amplified fragment length polymorphism (AFLP) method as molecular genetic technique, to detect AFLP band patterns as genetic markers, and to compare the efficiency of agarosegel electrophoresis (AGE) and polyacrylamide gel electrophoresis (PAGE), respectively. Using 9 primer combinations, a total of 141 AFLP bands were produced, 108 bands (82.4%) of which were polymorphic in AGE. In PAGE, a total of 288 bands were detected, and 220 bands (76.4%) were polymorphic. The AFLP fingerprints of AGE were different from those of PAGE. Separation of the fragments with low molecular weight and genetic polymorphisms revealed a distinct pattern in the two gel systems. In the present study, the average bandsharing values of the individuals between two populations apart from the geographic sites in Kangwon-do ranged from 0.084 to 0.738 of AGE and PAGE. The bandsharing values between individuals No.9 and No. 10 showed the highest level within population, whereas the bandsharing values between individuals No.5 and No.7 showed the lowest level. As calculated by bandsharing analysis, an average of genetic difference (mean$\pm$SD) of individuals was approximately 0.590$\pm$0.125 in this population. In AGE, the single linkage dendrogram resulted from two primers (M11+H11 and M13+H11), indicating six genetic groupings composed of group 1 (No.9 and 10), group 2 (No. 1, 4, 5, 7, 10, 11, 16 and 17), group 3 (No. 2, 3, 6, 8, 12, 15 and 16), group 4 (No.9, 14 and 17), group 5 (No. 13, 19, 20 and 21) and group 6 (No. 23). In AGE, the genetic distances among individuals of between-population ranged from 0.108 to 0.392. In AGE, the shortest genetic distance (0.108) displaying significant molecular differences was between individuals No.9 and No. 10. Especially, the genetic distance between individuals No. 23 and the remnants among individuals within population was highest (0.392). Additionally, in the cluster analysis using the PAGE data, the single linkage dendrogram resulted from two primers (M12+H13 and M11+H13), indicating seven genetic groupings composed of group 1 (No. 15), group 2 (No. 14), group 3 (No. 11 and 12), group 4 (No.5, 6, 7, 8, 10 and 13), group 5 (No.1, 2, 3 and 4), group 6 (No.9) and group 7 (No. 16). By comparison with the individuals in PAGE, genetic distance between No. 10 and No. 7 showed the shortest value (0.071), also between No. 16 and No. 14 showed the highest value (0.242). As with the PAGE analysis, genetic differences were certainly apparent with 13 of 16 individuals showing greater than 80% AFLP-based similarity to their closest neighbor. The three individuals (No. 14, No. 15 and No. 16) of rainbow trout between two populations apart from the geographic sites in Kangwon-do formed distinct genetic distances as compared with other individuals. These results indicated that AFLP markers of this fish could be used as genetic information such as species identification, genetic relationship or analysis of genome structure, and selection aids for genetic improvement of economically important traits in fish species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.727-743
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• 1996

To know the floristic composition, vortical distribution and community structure of marine benthic algae inhabiting in the intertidal and subtidal zones of Yongil Bay, east coast of Korea, the study has performed using the quadrat method along a transect line from July, 1995 to June, 1996. In this area, a total of 144 species including 2 new red algae to Korea was found: 5 blue-green, 18 green, 20 brown and 101 red algae. The representative species throughout the year were Ulva pertusa, Gelidium amansii and Symphyocladia latiuscula. Dominant species were Sargassum thunbergii in spring, U. pertusa in summer and autumn. In winter, Chondrus ocellatus and Monostroma grevillei occurred dominantly. The standing crop exhibited mean value as $185.8g/m^2$ dry weight. Maximum value was recorded in spring $(267.3g/m^2)$ and minimum was observed in winter $(93.7g/m^2)$. Shannon's species diversity (H') and evenness (J') as maximum value were recorded in spring, whereas minimum values were shown in winter. Vertical distribution, rerognized by cluster analysis based on relative coverage of the species, could be divided into two or three algal groups except spring. In general, green algae (M. grevillei, Capsosiphon fulvescens, U. pefusa, Enteromorpha compressa) and brown algae (Sargassum fulvellum, S. thunbergii) were represented in the upper and middle zone and red algae (G. amansii, C. ocellatus, S. latiuscula, Crateloupia okamurae, Pachymeniopsis eilliptica) in the lower zone. The algal community varied according to season and environmental conditions. Particularly, seasonal variation of vortical distribution seemed to be affected primarily by water temperature. Also seasonal tidal level and tolerance of algal species to desiccation appeared to be associated with it in this area.

• Journal of radiological science and technology
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• v.27 no.3
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• pp.31-41
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• 2004

Attention deficit hyperactivity disorder(ADHD)is one of the most common psychiatric disorders in childhood, especially school age children and persisting into adult. ADHD is affected 7.6% in our children, Korea. and persisting into $15{\sim}20%$ in adult. And it is characterized by hyperactivity, inattention and impulsivity. Brain imaging is one of way to diagnosis for ADHD. Brain imaging studies may be provide information two types - structural and functional imaging. Structural and functional images of the brain play an important role in management of neurologic and psyciatric disorders. Brain SPECT, with perfusion imaging radiopharmaceuticals is one of the appropriate test to diagnosis of neurologic and psychiatric diseases. Ther are a few studies about separated analysis between boys and girls ADHD SPECT brain images. Selection of Probability level(P-value) is very important to determind the abnormalities when analysis a data by SPM. SPM is a statistical method used for image analysis and determine statistical different between two groups-normal and ADHD. Commonly used P-value is P<0.05 in statistical analysis. The purpose of this study is to evaluation of blood flow clusters distribution, between boys and girls ADHD. The number of normal boys are 8(6-7y, average : $9.6{\pm}3.9y$) and 51(4-11y, average : $9.0{\pm}2.4$) ADHD patients, and normal girls are 4(6-12y, average : $9{\pm}2.4y$) and 13(2-13y, average $10{\pm}3.5y$) ADHD patiens. Blood flow tracer $^{99m}Tc-ethylcysteinate$ dimer(ECD) injected as rCBF agent and take blood flow images after 30 min. during sleeping by SPECT camera. The anatomical region of hyperperfusion of rCBF in boys ADHD group is posterior cingulate gyrus and hyperperfusion rate is 15.39-15.77% according to p-value. And girls ADHD group appears at posterior cerebellum, Lt. cerbral limbic lobe and Lt. Rt. cerebral temporal lobe. These areas hyperperfusion rate are 24.68-31.25%. Hypoperfusion areas in boys ADHD,s brain are Lt. cerebral insular gyrus, Lt. Rt. frontal lobe and mid-prefrontal lobe, these areas decresed blood flow as 15.21-15.64%. Girls ADHD decreased blood flow regions are Lt. cerebral insular gyrus, Lt. cerebral frontal and temporal lobe, Lt. Rt. lentiform nucleus and Lt. parietal lobe. And hypoperfusion rate is 30.57-30.85% in girls ADHD. The girls ADHD group's perfusion rate is more variable than boys. The studies about rCBF in ADHD, should be separate with boys and girls.

• Korean Journal of Environment and Ecology
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• v.25 no.2
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• pp.156-165
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• 2011

It is selected seven sites for marine algal flora and community structure and investigated seasonally from December 2008 to November 2009 in Gogunsan Islands, the west coast of Korea. A total of 58 species including 10 green, 16 brown and 32 red algae were collected and identified. Among these species, 11 species were found throughout the year. Annual mean biomass in dry weight were 213.8 g $m^{-2}$ at Sinsido 1, 143.1 g $m^{-2}$ at Sinsido 2, 133.3 g $m^{-2}$ at Sinsido 3, 164.0 g $m^{-2}$ at Munyeodo, 116.9 g $m^{-2}$ at Seonyudo, and 145.1 g $m^{-2}$ at Jangjado. Maximum biomass was recorded in Sinsido 1, and minimum mean biomass was Sinsido 4. The dominant species based on biomass were Sargassum thunbergii, Ulva pertusa, Sargassum fusiforme and Corallina pilulifera. S. thunbergii was the representative alga occurred at all seasons. The flora investigated could be classified into six functional groups such as coarsely branched form (46.6%), filamentous form (27.6%), sheet form (17.2%), thick leathery form (3.4%), jointed calcareous form (3.4%) and crustose form (1.7%). The R/P, C/P and (R+C)/P value reflecting flora characteristics were 0.33~0.75, 1.11~2.50, 1.47~3.25, respectively. Diversity index (H') and dominance index (DI) indicated that stability of seaweed community of Gogunsan Islands was unstable and environmental conditions were bad. According to multidimensional scaling (MDS) and cluster analysis, Gogunsan Islands were divided into three distinct groups. The first group was Sinsido 2, Sinsido 3, Seonyudo and Muyeodo and the second group comprised Sinsido 1 and Jangjado and the other was Sinsido 4 due to meaningful difference in similarity.

• Korean Journal of Poultry Science
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• v.45 no.3
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• pp.229-236
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• 2018

The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (${\Delta}K=7.96$). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.

• Journal of Bio-Environment Control
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• v.20 no.3
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• pp.211-215
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• 2011

This study aimed to determine optimum leaves per shoot in 'Campbell Early' grape through investigating changes of fruit quality after veraison 70~75 berries of first cluster and 35~40 berries of second cluster at 30 days after full bloom. Soluble solids content (SSC) increased dramatically during 28 days after veraison and afterwards it was stabilized. 15 leaves per shoot treatment showed fast increase of SSC from early veraison and reached to over 16.0oBx at 28 days after veraison. No considerable increase of SSC were occurred on either 5 or 0 leaves per shoot treatments after veraison. Titratable acidity (TA) was decreased continuously during 28 days of berry ripening in the all treatments. In 15 leaves per shoot treatment, TA decreased fast during first 7 days after veraison and continuously decreased to 0.5% by harvest time. While, 5 and 0 leaves per shoot treatments showed TA decrease slightly only at the early veraison. Hunter L and b values of 15 leaves per shoot treatment decreased fast during first 7 days of veraison, and gradually decreased by 14 days, whereas Hunter a value increased fast during the first 7 days from veraison. Total anthocyanin content in 15 leaves per shoot treatment increased fast from 7 days of veraison and the increase became slow after 28 days of veraison. Other treatments showed no significant difference from veraison to harvest time.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.345-354
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• 2013

To understand the community structures of the vegetation of the beaches and coastal dunes of Seocheon-gun, Boryeong-si, Anmyeon-do, Taean-gun in Chungcheongnam-do and Buan-gun in Jeollabuk-do at the west coast of South Korea, a plant sociological vegetation survey was carried out to make a classification of correlation vegetation communities, a cluster analysis and an inorganic component analysis on the soil and plant body. Regarding the vegetation of the coastal dunes, plant communities were classified into 31 communities by correlation dominant species, and as a result of a cluster analysis on the vegetation communities, they were classified into five vegetation types such as Calystegia soldanella community, Rosa rugosa community, Imperata cylindrica community, Vitex tend community and Elymus mollis community. The soil pH was highest in Seocheon area ($7.54{\pm}1.18$) while lowest in Buan area ($6.29{\pm}2.15$), and regarding inorganic components, the nitrogen content was highest in Robinia while lowest in Imperata cylindrica. The soil PH of vegetation I was highest (8.23) among the five vegetation types, and the organic matter was lowest (71.58). As a result of CCA ordination analysis to examine the correlation among environmental factors, Calystegia soldanella community, Rosa rugosa community and Imperata cylindrica community among the five vegetation types were distributed while they were not affected by soil environmental elements, but Vitex tend community and Elymus mollis community were distributed mainly at the places with high $K^+$ content in the soil.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.525-538
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• 2019

The purpose of this study was to analyze the habitat status and species diversity of benthic diatoms and estimate the applicability of TDI (Trophic Diatom Index) to obtain the basic data for the identification and management of created wetlands in the Nakdong River. We observed a total of 38 families and 173 species of benthic diatom during the survey period, and spring and autumn showed a similar number of species of 156 and 154, respectively. The result of the SOM (Self-Organizing Map) analysis showed that the distribution of benthic diatom was sensitive to environmental factors such as nutrient concentration and rainfall in each wetland. The cluster 1 was characterized by the survey sites of autumn mostly and consisted of points of high TDI, although the nutrients such as total phosphorus and total nitrogen were low, and the species number and abundance of diatoms were low. Conversely, cluster 4 was characterized by the survey sites of spring mostly and consisted of points of low TDI, even though total nitrogen was high. Considering that most of the created wetlands had the reduced inflow and outflow, the increased flow rate in the summer lowers nutrient values in autumn, and the species number and abundance of benthic diatom decreases due to the increase of turbidity, which reduces the light penetrations to the substrates. On the contrary, the TDI value is low in spring because the low water level causes insufficient substrate surface to the benthic diatoms, and it is too early for the establishment and development of saprophilous species. Although various studies have used TDI as an indicator for evaluating the habitat environment and water quality, it is not a good evaluation indicator in this study since the nutrient concentration in the wetlands mostly high as they have a low flow rate and are close to the stagnant area. Nevertheless, additional periodic surveys that comprehensively reflect the fact that the summer rainfall and inflow/outflow regulating function might affect the species diversity and distribution of benthic diatoms are necessary.

• Journal of agriculture & life science
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• v.50 no.1
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• pp.105-115
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• 2016

This study was carried out to establish the optimum forest management plan for the Cryptomeria japonica plantations in southern inland and Jeju island in Korea. Sixty seven circular sample plots of 0.04ha were established and we surveyed vegetation structure and growth characteristics from three layers(upper, middle, and lower). As a result of cluster analysis obtained by importance values of each tree species, the community type of C. japonica stands were classified into C. japonica group(C1) and C. japonica-C obtusa group. C. obtusa community were also sbudivided into P. thunbergii-Q. serrata group(C2) and Q. serrata-C obtusa group(C3). In tree layers importance value(IV) of C. japonica were 97.2% in C1, 80.7% in C2, and 47.6% in C3 and in sub-tree layers IV were 8.9% in C1, 15.2% in C2, and 5.7% in C3. Especially in C3 there are bamboo species (Smilacina japonica var. lutecarpa and Pseudosasa japonica) it is necessary for us to control them. In shrub layers C. japonica were found in C1(9.2%) and C2(7.0%), but except for C3. In tree layer species diversity indices of each community ranged from the lowest 0.059 in C1 to the highest 0.548 in C3. Dominance ranged from 0.958 in C1 to 0.393 in C3 which may caused by interspecific competition. Current annual increment of diameter growth ranged from 7.01mm/yr to 8.04mm/yr. As a result of our study we recommend the application of proper thinning and pruning for C1 and C2.

• Journal of Environmental Science International
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• v.23 no.2
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• pp.291-302
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• 2014

We have studied characteristics of community structure on the benthic macro-invertebrates at twelve selected sites in upstream, midstream and downstream of Paemsagol, Piagol, Chilseongol and Daeseonggol, major streams of Jirisan Mountain, for three years from April 2008 to September 2010. As a result, 2 phyla, 3 classes, 11 orders, 41 families, 119 species and 4,449 individuals of benthic macro-invertebrates have been collected and classified in this study. Among them EPT group (Ephemeroptera, Plecoptera, Trichoptera), commonly appearing taxa in clean stream ecosystem, was found most frequently. The highest species number of benthic macro-invertebrates were collected in 2008, the year with lower climatic influence including heavy rain. In almost all of survey sites 80% or over of EPT group were counted, and St. 1 scored highest rate of EPT group at 92.59%. Most dominant species was Ecdyonurus kibumensis at 12.83% of dominance index, and subdominant species was Goerodes KUb at 6.81% of dominance index. The investigation of the feeding function group indicated that gathering-collectors were dominant with 26 species and 1,334 individuals, while the number of filtering-collectors was lowest with 11 species and 230 individuals. This result represents that the feeding function group of Jirisan Mountain is mountainous stream-specific one. Both diversity index and richness index were lowest at the midstream of Chilseongol (St. 5), while the midstreams of Daeseonggol (St. 11) and Piagol (St. 8) were highest in diversity and richness indices respectively. The analyses of linear regression and correlation were performed in order to investigate and to predict the appearance aspect of EPT group by altitude. The results showed that the ratio of Plecopteran species number has increased by the elevation of the altitude at 0.05 significance level. Cluster analysis was also carried out for evaluating environmental similarities among survey sites. As a result, upper regions of Paemsagol (St. 1) and Piagol have clustered as most similar sites each other, while the midstream of Piagol (St. 8) has separated with lower similarity value than other sites in species composition.