• Title/Summary/Keyword: cluster method

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Analysis of Genetic Variation in Botrytis cinerea Isolates Using Random Amplified Polymorphic DNA Markers

  • Choi, In-Sil;Kim, Dae-Hyuk;Lee, Chang-Won;Kim, Jae-Won;Chung, Young-Ryun
    • Journal of Microbiology and Biotechnology
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    • v.8 no.5
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    • pp.490-496
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    • 1998
  • Random amplified polymorphic DNA (RAPD) markers were used to survey genetic variability among 34 Botrytis cinerea isolates from nine different host plants in Korea. For RAPD analysis, 115 arbitrary decamer primers were initially screened for polymorphic major DNA bands with 11 representative B. cinerea isolates. Eleven primers that initially detected polymorphisms were tested a second time with additional 23 isolates of B. cinerea as well as one isolate of Botrytis squamosa as an outgroup. The RAPD analyses revealed that all isolates except one showed different molecular phenotypes. Dendrograms obtained from dissimilarity matrices using the unweighted paired group method of arithmetic means (UPGMA) showed the 36.4% to 90.0% similarity among all B. cinerea isolates. The B. squamosa isolate showed the least similarity to all B. cinerea isolates. The cluster analyses indicated no correlation among all the characteristics examined including molecular phenotypes, host and geographic origins, year of isolation, or pathogenicity. The RAPD data suggest that a high level of genetic variation exists among Korean populations of B. cinerea and it seems to be caused by heterokaryosis among preexisting molecular phenotypes.

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Health Status, Reproductive Health Problems, and the Degree of Prenatal Management in Married Working Women (기혼 취업여성의 건강상태, 생식건강상태 및 산전관리상태)

  • Kim, Jeung-Im;Han, Seung-Hyun
    • Women's Health Nursing
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    • v.10 no.3
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    • pp.226-234
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    • 2004
  • Purpose: This study was to examine health status, reproductive health problems and the degree of prenatal management in working women. Method: The subjects were 902 married working women among 2,000 women selected by cluster sampling. The work area was classified to product factory, school, office, etc. After an Informed consent was obtained, participants were asked to fill out a self-administered questionnaire. The instruments included a questionnaire, parity check list, menstrual problems and gynecologic problem check list. Result: Many women have experienced menstrual cycle change during the past year. There was a significant difference in general health, menstrual regularity, reproductive health and prenatal management by occupation type. 40.6 percent of the subjects have gynecological problems such as menstrual cycle change, perineal inflammation, irregular vaginal bleeding, amenorrhea, ovarian/uterine disease, infertility, or abortion. Prenatal care was received in only 28.5% of the total subjects. In addition, 16.3% answered they had experienced low birth weight babies. Conclusion: We can conclude that working woman have changes in menstruation cycle and in reproductive health status. Therefore, we suggest that some occupational characteristics may affect subject's reproductive health, these should be clear and avoided as much as possible.

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THE MORPHOLOGY OF CHROMIUM AND LIF MEASUREMENT OF ATOMIC ARSENIC IN LAMINAR DIFFUSION FLAMES

  • Yoon, Young-Bin
    • 한국연소학회:학술대회논문집
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    • 1997.06a
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    • pp.61-68
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    • 1997
  • The morphology and size distribution of chromium oxides and the concentration measurement of atomic arsenic have been studied in laminar diffusion flames. Nitrogen was added to vary flame temperatures in hydrogen flames. Ethene flames were used in order to investigate the potential for interaction between the soot aerosol that is formed in these flames and the chromium aerosol. Two sources of chromium compounds were introduced: chromium nitrate and chromium hexacarbonyl. A detailed investigation of the morphology was carried out by scanning electron microscopy (SEM). The amounts of Cr(VI) and total Cr were determined by a spectrophotometric method and by X-ray fluorescence spectrometry, respectively. Also, LIF was used for the measurement of atomic arsenic, which was excited at 197.2 nm and was detected at 249.6 nm. Results showed that the morphology of the particles varied with the flame temperature and with the chromium source. The particles were characterized by porous structures, cenospheres and agglomerated dense particles when chromium nitrate solution was added to the flames. At low to moderate temperatures, porous sintered cenospheric structures were formed, in some cases with a blow hole. At higher temperatures, an agglomerated cluster which was composed of loosely sintered submicron particles was observed. It was also found that the emission of Cr(VI) from the undiluted $H_2$ flame was more than 10 times larger than in the 50% $H_2$ / 50% $N_2$ flame on a mass basis. Single point LIF measurement of atomic arsenic indicated that arsenic exist only in the low temperature, fuel rich region.

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Children's Wear Purchasing Behavior by Retired Women and the Missy Group with a View to their Shopping Orientation (의복쇼핑성향에 따른 집단별 유아동복 구매행동의 세대 간 차이 -뉴실버세대와 신세대 주부를 중심으로-)

  • Chung, You-Jin;Hwang, Choon-Sup
    • Journal of the Korean Society of Clothing and Textiles
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    • v.36 no.1
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    • pp.84-98
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    • 2012
  • This study examines the children's wear purchasing behavior of younger generation women (the missy group) and in the new-silver generation (retired). The data collected in the study were examined with a view to establish effective marketing strategies within the children's wear market, a market where the age and characteristics of the actual purchasers of the products have become more diverse. A descriptive survey method using a self-administered questionnaire was employed. The sample consisted of 398 females between the ages of 28 and 64 residing in Seoul and the Gyeonggi-do area. Data collected were analyzed by frequency, t-test, ANOVA, factor analysis, cluster analysis, and Cronbach's alpha coefficients. Respondents were classified into 4 groups by their clothes shopping orientation: conformable/brand conscious purchasing type, planned purchasing/enjoy shopping type, store/brand loyal impulse-oriented type, and dependent/low shopping interest type. There were differences between the missy and the new- silver generations under consideration. It is clear from the study that, even though some individuals of the missy group and of new-silver group belonged to the same shopping orientation type, individuals still showed differences with regard to children's wear purchasing behavior.

Comparison of Cardiovascular Risk Profile Clusters Among Industrial Workers

  • Hwang, Seon-Young;Lee, Ji-Hyun
    • Journal of Korean Academy of Nursing
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    • v.35 no.8
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    • pp.1500-1507
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    • 2005
  • Purpose. The purpose of this study was to identify subgroups of the physical and behavioral risk profiles for cardiovascular disease among industrial workers, and to examine predicting factors for the subgroups. Sample and Method. Health records of 2,616 male and female workers aged 19-56 years who were employed in an airplane manufacturing industry were analyzed. Data were analyzed using the Latent class cluster analysis. Results. Four different clusters (two high-risk groups, one low-risk group, and one normal group) were found and these clusters were significantly different by age, gender, and work type (p < .05 ). The two high-risk groups had higher chances of drinking alcohol, elevated BMI, FBS, total cholesterol, having hypertension, and were significantly older, and had relatively high chances of being day workers rather than other groups. The low-risk group had higher chances of drinking alcohol, higher BMI and total cholesterols compared to normal group, and highest portions of current smokers and shift workers in the four clusters and their mean BP was within prehypertension criteria. Conclusion. Industrial nurses should guide the lifestyle behaviors and risk factors of the high risk groups for CVD and need to intervene early for behavioral change for the low-risk group who are young and shift workers. Age, and work environment should be considered in planning for targeted preventive interventions for industrial workers.

Factors Influencing Coping Strategies of Elders According to Types of Pain Belief (노인의 통증신념 유형에 따른 통증대처 전략 영향요인)

  • Kim, Geun-Myun;Chang, Sung-Ok;Lee, Yong-Mi
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.15 no.3
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    • pp.360-370
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    • 2008
  • Purpose: This study was done to identify factors (pain stress, perceived stress, pain disability, fatigue, depression) strategies used by elders to cope with pain based on their type of pain belief. Method: Data were collected from 314 elders in community settings in Seoul from September to December of 2007. Cluster analysis, t-test, and ANOVA were used to analyze data. Result: The types of pain belief were classified as the following groups: Self-blame, Enduring & Mysterious, and Short-term & Understandable. Perceived stress (t=2.36, p=.02), social support (t=2.24, p=.03), extent of pain relief (t=2.39, p=.02), and duration of pain relief (t=2.09, p=.04) were important factors for active and passive coping in the Self-blame group. Pain stress (t=2.39, p=.01) and depression (t=-3.99, p=.00) were significantly related to the active coping in the Enduring & Mysterious group. Perceived stress (t=2.55, p=.01) was an important factor in the passive coping in the Short-term & Understandable group. Conclusion: Considering different types of pain belief in elders and factors that are significantly related to different coping strategies, future nursing interventions should be population specific to encourage active coping strategies and to decrease passive coping strategies.

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Anger, Problem Behaviors, and Health Status in Adolescent Women (청소년기 여성의 분노와 문제행동 및 건강상태)

  • Park Young-Joo;Han Keum Sun;Shin Hyun Jeong;Kang Hyun-Chul;Moon So-Hyun
    • Journal of Korean Academy of Nursing
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    • v.34 no.7
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    • pp.1234-1242
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    • 2004
  • Purpose: This cross-sectional study was designed to identify anger-expression types of adolescent women and investigate the relation between the identified anger-expression types and their problem behaviors and health status. Method: One hundred ninety nine high school freshmen were recruited from September to November, 2003. Data was analyzed using descriptive statistics, cluster analysis, 2-test, ANOVA, and Duncan's multiple comparison test. Result: Three anger-expression types in adolescent women were found; Anger-out/in, Anger-control/in, and Anger-control type. Adolescent women with frequently using the anger-out/in type and with higher state anger reported more delinquent behaviors, more health risk behaviors, and higher psychosomatic symptoms. However, adolescent women with lower state anger and frequently using the anger-control type reported more depression scores. Conclusion: There is a need to further clarify the relationship between anger-expression type sand depression in adolescent women. The findings suggest the necessity of a development of the program for lowering the anger level and controlling the unfavorable anger expression types such as the anger-out in.

Towards Conservation of Threatened Ceropegia Species Endemic to a Biodiversity Hotspot: In Vitro Microtuber Production and Proliferation, a Novel Strategy

  • Pandit, Sagar Subhash;Nair, Aneeshkumar;Naik, Dhiraj Dilip
    • Journal of Forest and Environmental Science
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    • v.24 no.2
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    • pp.79-88
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    • 2008
  • Twenty-eight of 44 Indian Ceropegia species are endemic and their survival is threatened. As a step towards conservation, we implied in vitro methods for the sustainable propagule production in C. hirsuta, C. lawii, C. maccannii, C. oculata and C. sahyadrica. Effects of explant, growth regulators, sucrose and photoperiod were studied. High frequency microtuber production was achieved with the seedling-apical buds, grown on MS medium containing 4-6 mg $1^{-1}$ BAP, 3-8% (w/v) sucrose, under continuous illumination. Each microtuber, when subcultured proliferated to form a cluster of secondary microtubers. Every primary and secondary microtuber bore at least one shoot-bud and a root primordium. Each tuber (formed with any of the significantly effective treatments) weighed more than 500 mg, enough to plant directly in non-sterilized soils. Microtubers could be produced and proliferated round the year. Proliferation could be solely attributed to in vitro procedures as these plants bear solitary tubers in vivo. Microtubers could be sprouted in vitro to prepare ready to pot plantlets. As, this novel method succeeded for all five species, though they belong to different eco-physiological backgrounds, we recommend its implementation in the conservation programs for a broader range of Ceropegia species, supported by other integrated strategies.

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Artificial neural network algorithm comparison for exchange rate prediction

  • Shin, Noo Ri;Yun, Dai Yeol;Hwang, Chi-gon
    • International Journal of Internet, Broadcasting and Communication
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    • v.12 no.3
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    • pp.125-130
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    • 2020
  • At the end of 1997, the volatility of the exchange rate intensified as the nation's exchange rate system was converted into a free-floating exchange rate system. As a result, managing the exchange rate is becoming a very important task, and the need for forecasting the exchange rate is growing. The exchange rate prediction model using the existing exchange rate prediction method, statistical technique, cannot find a nonlinear pattern of the time series variable, and it is difficult to analyze the time series with the variability cluster phenomenon. And as the number of variables to be analyzed increases, the number of parameters to be estimated increases, and it is not easy to interpret the meaning of the estimated coefficients. Accordingly, the exchange rate prediction model using artificial neural network, rather than statistical technique, is presented. Using DNN, which is the basis of deep learning among artificial neural networks, and LSTM, a recurrent neural network model, the number of hidden layers, neurons, and activation function changes of each model found the optimal exchange rate prediction model. The study found that although there were model differences, LSTM models performed better than DNN models and performed best when the activation function was Tanh.

Effect of Long Term Fertilization on Microbial Biomass, Enzyme Activities, and Community Structure in Rice Paddy Soil

  • Lee, Chang Hoon;Kang, Seong Soo;Jung, Ki Youl;Kim, Pil Joo
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.6
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    • pp.487-493
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    • 2013
  • The effects of long-term fertilization on soil biological properties and microbial community structure in the plough layer in a rice paddy soil in southern Korea were investigated in relation to the continuous application of chemical fertilizers (NPK), straw based compost (Compost), combination these two (NPK + Compost) for last 40 years. No fertilization plot (Control) was installed for comparison. Though fertilization significantly improved rice productivity over control, the long-term fertilization of NPK and compost combination was more effective on increasing rice productivity and soil nutrient status than single application of compost or chemical fertilizer. All fertilization treatments had shown significant improvement in soil microbial properties, however, continuous compost fertilization markedly increased soil enzyme and microbial activities as compared to sole chemical fertilization. Results of microbial community structure, evaluated by EL-FAME (ester-linked fatty acid methyl esters) method, revealed big difference among Control, NPK, and Compost. However, both Compost and Compost+NPK treatments belonged to the same cluster after statistical analysis. The combined application of chemical fertilizer and organic amendments could be more rational strategy to improve soil nutrient status and promote soil microbial communities than the single chemical fertilizer or compost application.