• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1928-1934
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• 2006

The rubradirin biosynthetic gene cluster harbors 58 ORFs within a 105.6-kb sequence, which includes all of the genes responsible for the synthesis of rubradirin, as well as the primary genes relevant to regulatory, resistance, and transport functions. This gene cluster also harbors a resistance-mediating ABC transporter, RubT1, which is located at the most upstream position in the cluster. In the present study, RubT1 was expressed heterologously in E. coli, and the resistance affinity of RubT1 was determined by an antibacterial activity test, as well as by HPLC and ESI-MS analyses. Evidence clearly demonstrates that RubTl mediates rubradirin resistance as an ABC transporter.

• The Korean Journal of Community Living Science
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• v.27 no.1
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• pp.95-108
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• 2016

The objectives of this study are to analyze the characteristics of multi-cultural families in rural areas and to classify their types by applying multi-variate techniques and cluster analyses. Data for the study were obtained by a surveying 120 married migrant females in rural areas of Chungchengnam-do, South Korea. By utilizing the factor analysis to analyze the characteristics of multi-cultural families, 16 basic variables related to these female subjects were categorized into 6 factors: 'marriage length and age', 'language skill of migrant female', 'language skill of husband', 'family satisfaction', 'income and education', and 'general living satisfaction in Korea with remittance'. By appling the cluster analysis, multi-cultural families in rural areas were divided into the following 5 types: 'stable settlement', 'average but stagnant', 'below average yet positive', 'high- income with little communication', and 'young low-income'. In all types, it is strongly recommended to develop various programs regarding vocational education for the migrant females in order to increase their economic opportunities as well as social status.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.8-16
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• 2008

Aurofusarin is a polyketide pigment produced by some Fusarium species. The PKS12 and GIP1 genes, which encode a putative type I polyketide synthase (PKS) and a fungal laccase, respectively, are known to be required for aurofusarin biosynthesis in Gibberella zeae (anamorph: Fusarium graminearum). The ten additional genes, which are located within a 30 kb region of PKS12 and GIP1 and regulated by a putative transcription factor (GIP2), organize the aurofusarin biosynthetic cluster. To determine if they are essential for aurofusarin production in G. zeae, we have employed targeted gene deletion, complementation, and chemical analyses. GIP7, which encodes O-methyltransferase, is confirmed to be required for the conversion of norrubrofusarin to rubrofusarin, an intermediate of aurofusarin. GIP1-, GIP3-, and GIP8-deleted strains accumulated rubrofusarin, indicating those gene products are essential enzymes for the conversion of rubrofusarin to aurofusarin. Based on the phenotypic changes in the gene deletion strains examined, we propose a possible pathway for aurofusarin biosynthesis in G. zeae. Our results would provide important information for better understanding of naphthoquinone biosynthesis in other fdarnentous fungi as well as the aurofusarin biosynthesis in G. zeae.

• The Korean Journal of Ecology
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• v.23 no.6
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• pp.453-460
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• 2000

The forest vegetation of Mt. seondal was classified into eight communities and one afforestation by the phytosocialogical method (Z-M method). In general, Quercus mongolica trees occupied most of the area, while afforestation forest was distributed on the lower slope, cultivated land, and at the vicinity of village. The vegetation on the top part of Mt. Seondal was comparatively well preserved, but that in the lower areas has been disturbed heavily by human activity and some had mixed forests composed of pine trees, oaks, ashes, and Rhododendron micrantum shrub. By cluster analysis method. nine groups were identified as follows : Quercus mongolica group, Q. mongolica - Pinus densiflora group, Q. mongolica - Rhododendron schlipen - bachii group, Q. mongolica - Symplocos chinensis for. pilosa group, P. densiflora group, Juglans mandshurica group, Fraxinus mandshurica group, Betula costata group and Larix leptolepis group. These groups showed differences in species composition, but Quercus mongolica, Q. mongolica - P. densiflora, Q. mongolica - R. schlippenbachii and Q. mongolica - S. chinensis for. pilosa groups among them showed very similar floristic composition to each other. In the relationship between polar ordination axes and environmental variables, altitude was the environmental factor determining variation in species composition along axis X and soil moisture was the environmental along axis Y. They were the main factors in determining forest vegetation. The result of cluster analysis and polar ordination for the forest vegetation were corresponded to those of phytosocialogical classification in classifying vegetation.

• Journal of the Korean Home Economics Association
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• v.48 no.8
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• pp.1-12
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• 2010

The purpose of this study was to explore and examine the relationship between mother-child emotional availability and preschoolers' story stem narrative response. Eighty two 4-year-old preschoolers and their mothers participated in this study. This study used the following translated evaluation tools: Emotional Availability Scales(EA, 3rd edition) designed by Biringen and colleagues(1998) to examine the quality of the emotional relationship between mother and her child, and the MacArthur Story Stem Battery(MSSB) developed by Bretherton and colleagues with the MacArthur Narrative Group(1990) to measure preschoolers' narrative responses. The following statistical analyses were preformed descriptive, cluster analysis, and One-way ANOVA. The findings were as follows: First, 4 clusters of the narrative responses of 4-year-old preschoolers were prosocial story tellers, avoidant/dysregulated story tellers, constrained story tellers, and anxious story tellers. Second, the preschoolers in the prosocial cluster showed a high level of mother-child emotional availability and the preschoolers in the avoidant/dysregulated cluster showed a low level of mother-child emotional availability.

• Korean Journal of Microbiology
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• v.43 no.2
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• pp.137-141
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• 2007

The similarities among five strains of Streptomyces were measured by the serological methods. Antigens were prepared by dissolving cell envelope fractions in Tween 20 buffer and antisera were produced from the immunized rabbits. Immunodiffusion studies and ELISA results showed that the degree of antigen-antibody reaction was not exactly matched to the taxonomic distance; i. e. the strains of the cluster group A exhibited low level of cross-reactions each other, while relatively strong cross-reactions were observed between Streptomyces lavendulae of cluster group F and Streptomyces viridochromogenes of cluster group A.

• Journal of Korean Society for Atmospheric Environment
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• v.30 no.5
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• pp.504-509
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• 2014

Twenty-five stations of the urban monitoring network in Seoul were assessed with a focus on surveillance function of the exceedances of 8-hour ozone and 24-hour $PM_{10}$ standards. The two standards were selected because their attainment rates were particularly low. Two hierarchical cluster analyses were performed to group stations with similar atmospheric environments-one using daily highest 8-hour [$O_3+NO_2$], 8-hour $O_3$ concentrations plus corresponding 8-hour $NO_2$ concentrations considering the interconversion of $O_3$ and $NO_2$, and the other using 24-hour $PM_{10}$ concentrations. An index to measure higher concentration and exceedances of the standards was introduced. Within a cluster, sufficiently high score was assigned to the trends station or the station with higher index. Scores for $O_3+NO_2$ and $PM_{10}$ of a given station were added and ranked in the descending order to determine the relative importance.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.4
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• pp.405-410
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• 1996

Phylogenetic analyses were carried out using four Indonesian native chicken breeds; Kampung, Bangkok, Pelung and Kedu. Gene frequencies of four blood group (A, B, D and E) and eight electrophoretic loci (akp, Akp-2, Es-1, Amy-1, Alb, Tf, Pas and Pa-1) were examined. Geographical and breed specific trends in the gene frequencies were not found in the local population of Kampung breed or in four native breeds. The values of average heterozygosity were estimated as 0.35-0.45. Genetic distances among the local populations of Kampung breed and other native breeds were comparatively small. In a cluster analysis, the Bangkok breed and Kampung E population showed distance from another cluster. The coefficient of gene differentiation for local populations of Kampung breed was estimated as 0.099.

• Journal of Environmental Science International
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• v.8 no.3
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• pp.331-336
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• 1999

The monthly water quality data measured at 14 stations located in the Geum-River watershed were clustered into 2 to 7 clusters. Furthermore, factor analyses were conducted on Gabcheon and Yugucheon to characterize the water qualtiy, based on the information obtained from the results of culster analysis. The results of cluster analysis show that the water quality charactersitic of main stream of the Geum-River is somewhat different from that of substream of the Geum-River. Furthermore, the water quality characteristic of Gabcheon which is expected to have the most serious water quality problems in the Geum-River watershed shows the most different water quality characteristic from Yugucheon. Based ont he factor loadings in each factor, Gabcheon and Yugucheon have their own water quality characteristics. This is mainly because of composite factors such as different population density, industrial activities, and land use conditions in Gabcheon and Yugucheon subwatersheds.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.382-387
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• 2011

The chemical composition of essential oils obtained from aerial parts in spearmint, apple mint and chocolate mint, was investigated by gas chromatography/mass spectrometry analyses. (-)-Carvone (33.0%) was quantitatively major compound in spearmint, followed by R-(+)-limonene (11.7%) and ${\beta}$-phellandrene (9.7%); (-)-carvone (37.4%) and germacrene D (11.9%) in apple mint; and (-)-menthol (34.3%), p-menthone (18.4%) and menthofuran (9.8%) in chocolate mint. Hierarchical cluster analysis and principle components analysis showed the clear difference in chemical composition of the three mint oils.